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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: NumeroEno]
    #21141131 - 01/18/15 08:02 PM (9 years, 1 month ago)

Quote:

EnoRoxxx said:
I've been running my mini rounds through the dishwasher because it makes the lids easier to remove. As for the hard water thing, it's all about a good detergent. My jars come out of the PC covered in shit that I regularly have to scrub off, but they come out spotless from the dishwasher. Even the city water here is really hard.




Why don't you put a tablespoon or three of white vinegar in your PC before you run it then, so that doesn't happen? :naughty:


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InvisibleNumeroEno
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #21141152 - 01/18/15 08:05 PM (9 years, 1 month ago)

Quote:

Inocuole said:
Quote:

EnoRoxxx said:
I've been running my mini rounds through the dishwasher because it makes the lids easier to remove. As for the hard water thing, it's all about a good detergent. My jars come out of the PC covered in shit that I regularly have to scrub off, but they come out spotless from the dishwasher. Even the city water here is really hard.




Why don't you put a tablespoon or three of white vinegar in your PC before you run it then, so that doesn't happen? :naughty:




I've thought about that, but haven't done it. I thought vinegar and aluminum were pretty reactive together. It won't oxidize the inside of my PC?


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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: NumeroEno]
    #21141154 - 01/18/15 08:06 PM (9 years, 1 month ago)

I thought that was specifically the point, to keep the foil particles from getting all over your jars?  My jars always come out clean so long as I remember to add the vinegar.


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InvisibleNumeroEno
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #21141165 - 01/18/15 08:09 PM (9 years, 1 month ago)

Interesting. Chemistry has always been my weakest subject in the science world.


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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: NumeroEno]
    #21141175 - 01/18/15 08:11 PM (9 years, 1 month ago)

I haven't noticed anything weird, though, other than maybe my foil's a little weaker than it should be after a PC run.  After 2-3 it's downright frail, and very dull looking, and the texture is highly unpleasant.  I think that happens without the vinegar too though, right?


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InvisibleNumeroEno
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #21141191 - 01/18/15 08:15 PM (9 years, 1 month ago)

I've never reused foil., but I do recycle it all. I guess diluted vinegar wouldn't have any effect on the inside of my PC. I'm gonna try some vinegar in my next PC run. If it has no effect on the inside of my PC and my jars aren't covered in calcium deposits I'll be really happy.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: NumeroEno]
    #21141215 - 01/18/15 08:20 PM (9 years, 1 month ago)

I think those are aluminum deposits.  If there was that much calcium in water I wouldn't ever have to drink milk.  Not that I have to drink milk.  In fact I probably shouldn't.  But I digress.


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OfflinetaGyo
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #21141271 - 01/18/15 08:35 PM (9 years, 1 month ago)

Quote:

taGyo said:
I wipe it with tap water on a paper towel just to get the extra agar out and make sure the bottom is completely clean.



Quote:

Pastywhyte said:
I don't even use iso, a tiger drop into the trash and a damp paper towel to get any residue off. Done.



Quote:

Inocuole said:
Yeah, completely forgot to chime in on that, I do the same, except I tiger drop them outside in a bush, before hitting em with the moist paper towel. :lol:



Quote:

MudaFuka said:
I just wipe mine off. they get PCed anyway.



:fuckinawesome:


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OfflineMushroom_J
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Re: Pastywhyte's Easy Agar Tek [Re: taGyo]
    #21141947 - 01/18/15 10:54 PM (9 years, 1 month ago)

A couple of my cups tipped in the PC... So i microwaved em' for 10 sec each, and they melted back into place.
We'll see how it goes lol with contams.


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Invisiblewowimflabbergasted
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Re: Pastywhyte's Easy Agar Tek [Re: Mushroom_J]
    #21141955 - 01/18/15 10:56 PM (9 years, 1 month ago)

Quote:

Mushroom_J said:
A couple of my cups tipped in the PC... So i microwaved em' for 10 sec each, and they melted back into place.
We'll see how it goes lol with contams.



Did the same thing and it worked fine.


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OfflineMushroom_J
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Re: Pastywhyte's Easy Agar Tek [Re: wowimflabbergasted]
    #21142136 - 01/19/15 12:03 AM (9 years, 1 month ago)

Cool beans.
In labs they make a bunch and leave what's unused in their flask... then microwave it when they need to pour. Think it lasts up to 3 months this way.

Figured it would work.. was concerned about heat drawing air in through the filter. My mind is at ease :thumbup:


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Invisibleelasticaltiger
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Re: Pastywhyte's Easy Agar Tek [Re: Mushroom_J]
    #21142163 - 01/19/15 12:12 AM (9 years, 1 month ago)

I have also heard of this.

Quote:

Mushroom_J said:
Cool beans.
In labs they make a bunch and leave what's unused in their flask... then microwave it when they need to pour. Think it lasts up to 3 months this way.

Figured it would work.. was concerned about heat drawing air in through the filter. My mind is at ease :thumbup:




But it makes me wonder how, once they open it to pour, isn't the agar exposed to contamination?

Then again I talked to a microbio-major and he said that 'contamination is always expected'

We always go for clean spawn but in a general-every-day bio lab some contamination is always expected on plates.


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Re: Pastywhyte's Easy Agar Tek [Re: elasticaltiger]
    #21142202 - 01/19/15 12:24 AM (9 years, 1 month ago)

Not sure either... antibiotic agar... good technique... speed.
Maybe mold contams aren't a problem in labs like bacterial contams
I've opened the same plate 5 times with no problem.... i've also opened them once and ruined everything lol.


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Re: Pastywhyte's Easy Agar Tek [Re: Mushroom_J]
    #21142940 - 01/19/15 08:48 AM (9 years, 1 month ago)

Contamination is expected?

What the fuck, that's bio-chem, they shouldn't be talking like that :lol:


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Re: Pastywhyte's Easy Agar Tek [Re: taGyo]
    #21143052 - 01/19/15 09:19 AM (9 years, 1 month ago)

Apparently I have a lower contam rate than said labs :shrug:


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Re: Pastywhyte's Easy Agar Tek [Re: wowimflabbergasted]
    #21143108 - 01/19/15 09:38 AM (9 years, 1 month ago)

Quote:

wowimflabbergasted said:
Quote:

Mushroom_J said:

A couple of my cups tipped in the PC... So i microwaved em' for 10 sec each, and they melted back into place.
We'll see how it goes lol with contams.



Did the same thing and it worked fine.




Damn, that's a good idea.  I have mycelium growing all around a strange blob of agar cause a few of mine tipped also


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Re: Pastywhyte's Easy Agar Tek [Re: DredgeMyEyes]
    #21143213 - 01/19/15 10:02 AM (9 years, 1 month ago)

Quote:

DredgeMyEyes said:
Quote:

wowimflabbergasted said:
Quote:

Mushroom_J said:

A couple of my cups tipped in the PC... So i microwaved em' for 10 sec each, and they melted back into place.
We'll see how it goes lol with contams.



Did the same thing and it worked fine.




Damn, that's a good idea.  I have mycelium growing all around a strange blob of agar cause a few of mine tipped also




Bloody hell, ive been growing culture on wonky agar for months......:dumblol:  Such a simple fix......D'oh


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Invisiblewowimflabbergasted
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Re: Pastywhyte's Easy Agar Tek [Re: Edmunter]
    #21143274 - 01/19/15 10:19 AM (9 years, 1 month ago)

It liquifies really quick so be careful not to do it too long. 10 seconds is usually good...I just stop the microwave once I see it melt down. :thumbup:


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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #21146675 - 01/19/15 09:16 PM (9 years, 1 month ago)

i just bought a PC... stupid question.... how much water do i need in it to sterilize my agar plates if stacked?


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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: qwobbler]
    #21146701 - 01/19/15 09:22 PM (9 years, 1 month ago)

Not much you just need a couple inches of water and elevate your plates above the water line with something like jar rings.


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