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Offlinejohnnytheswede
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Expanding cardboard spawn exponentially?
    #21134701 - 01/17/15 04:59 PM (9 years, 3 months ago)

Hi Forum,

I have been thinking of starting a wood-lover cultivation outside this spring. I have a spore syringe and a lot of cardboard (I have recently moved house).

My idea is the following: Cut a number of cardboard squares maybe a hundred with the side of 1/2'. Dilute the spores from the syringe and spread the spores onto the cardboard (cardboard has been hydrated first of course). Let the spores germinate and mycelium colonize the whole sides of two sheets, producing two sides between which a new square can be sandwiched. Then the following iterative process is initiated:

1. Take 1 new square and fit it between the two colonized sides, let full colonization take place. If I symbolize a colonized square with a "c" and uncolonized square with a "u" this step could be illustrated like this:
cuc ---> ccc

2. Take 2 new squares and fit between the four colonized square sides that are on the three squares produced in step 1. Await full colonization.
cucuc ---> ccccc

3. Take 4 new square and sandwich them in, just in analogy with step 2.
cucucucuc ---> ccccccccc

4. Take 8 new squares ...
cucucucucucucucuc ---> ccccccccccccccccc

5. Take 16 new squares ...
cucucucucucucucucucucucucucucucuc ---> ccccccccccccccccccccccccccccccccc

6. Take 32 new squares ...

and so on. This is exponential growth and if it works it could produce very much cardboard spawn in a short time. Do you think so? If so, could it be used to inoculate sawdust/chips directly thus obsoleting grain jars? I read somewhere that wood lovers grow slowly on grain but fast on cardboard.

I'd be very happy to hear about things that would be needed for this to work, I have a laminar flow hood that I could do the expansion (sandwiching procedure) in front of. One thing that inspired me to think up this is that I heard or read somewhere that cardboard is inherently resistant to mold.

Kind regards
Johnny


--------------------
Just learning to cultivate mushrooms, immensely impressed by Paul Stamets ("Pair up with Fungi!") and the knowledge and helpfulness at The Shroomery. Concentrating on Gourmet and Medicinal Mushrooms: Reishi, Shiitake, Garden Giant, Lion's Mane, Turkey Tail.

Acronyms (for noobs like me): http://www.shroomery.org/forums/showflat.php/Number/5875173#Post5875173
Have opted out of rating system because of personal reasons, read my bio if you are interested in why. (I opted out before receiving or giving any ratings, meaning no offense to anyone.)

Edited by johnnytheswede (01/17/15 05:02 PM)

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OfflineNos-hroom
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Re: Expanding cardboard spawn exponentially? [Re: johnnytheswede]
    #21134733 - 01/17/15 05:13 PM (9 years, 3 months ago)

I think that with my quick read-through of this I grasp the concept. However, you can inadvertently "Tire out" a single sample of mycelium stretched out over too much media.

Edit for clarification: If you were planning to do this indefinitely, it may be futile.

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OfflinecronicrFacebook
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Re: Expanding cardboard spawn exponentially? [Re: Nos-hroom]
    #21134741 - 01/17/15 05:16 PM (9 years, 3 months ago)

that is not a good way to go, check out pasty's easy agar tek first, wild spores harbour contams and spores won't germinate on cardboard,your best shot is agar to grain spawn to woodchips


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Offlinejohnnytheswede
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Re: Expanding cardboard spawn exponentially? [Re: cronicr]
    #21134785 - 01/17/15 05:34 PM (9 years, 3 months ago)

Thank you for your replies.

OK, well if spores wont germinate on cardboard and if the strain gets tired out from being stretched out, I guess that settles it.

I'll do it the conventional way.

Kind regards
Johnny


--------------------
Just learning to cultivate mushrooms, immensely impressed by Paul Stamets ("Pair up with Fungi!") and the knowledge and helpfulness at The Shroomery. Concentrating on Gourmet and Medicinal Mushrooms: Reishi, Shiitake, Garden Giant, Lion's Mane, Turkey Tail.

Acronyms (for noobs like me): http://www.shroomery.org/forums/showflat.php/Number/5875173#Post5875173
Have opted out of rating system because of personal reasons, read my bio if you are interested in why. (I opted out before receiving or giving any ratings, meaning no offense to anyone.)

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OfflineNos-hroom
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Re: Expanding cardboard spawn exponentially? [Re: johnnytheswede]
    #21136136 - 01/17/15 10:55 PM (9 years, 3 months ago)

There's no need to think about it like that once you've got things going, man.

Just stick to tried and true methods just for as many runs as you can stand. Honestly, I feel that anyone with slanted, trusted cultures and their own tried-and-true technique only does a service to our community by trying the unconventional or absurd (so long as they document it truthfully). Innovation and simply tweaking around methods just to see what happens is how some of the most unlikely and beautiful discoveries can be made.

Also, it's not necessarily the strain getting tired out, but more of the mycelium as a large organism "aging." You can keep a slanted culture around for years and, with the proper technique, stretch out a biologically "young" monoculture to hundreds of grows.

There are weeks worth of good material to read up on to answer seemingly countless questions.

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OfflineThe Librarian
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Re: Expanding cardboard spawn exponentially? [Re: johnnytheswede]
    #21136574 - 01/18/15 01:48 AM (9 years, 3 months ago)

All I know is that the sooner you get it on wood media the better.

:goodluck:


--------------------
Spore prints or it didn't happen.           Consolidate is a fancy word for: "If you touch it some more any time soon you'll kill it all."                              Great Scorpions Song
       

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Offlinejohnnytheswede
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Re: Expanding cardboard spawn exponentially? [Re: Nos-hroom]
    #21136685 - 01/18/15 03:50 AM (9 years, 3 months ago)

Thank you very much for your replies.

Quote:

Nos-hroom said:
Just stick to tried and true methods just for as many runs as you can stand. Honestly, I feel that anyone with slanted, trusted cultures and their own tried-and-true technique only does a service to our community by trying the unconventional or absurd (so long as they document it truthfully). Innovation and simply tweaking around methods just to see what happens is how some of the most unlikely and beautiful discoveries can be made.



Yes I agree completely, I believe that I will run tried and tested methods a lot but I also have the inclination to experiment, this first time I will probably do it conventionally, but later on I will not be able to resist the curiosity. I do make an effort to document what I do by being a bit comprehensive in my write ups. I trust that the posts that we create together will help many others.

Quote:

And The Librarian said:
All I know is the sooner I get it on wood the better.




This is what I thought that I'd use, Paul Stamets describes how the wood lover I have grows slow agar but on cardboard or wood it rhizomorphs strongly ... could one idea be to do sawdust/wood chip jars instead of grain jars?

I'll probably have grain jars in parallel too, but it can't hurt trying with a sawdust/wood chip jar, or is there some difficulty with handling wood in jars that I do not yet know of?

Kind regards
Johnny


--------------------
Just learning to cultivate mushrooms, immensely impressed by Paul Stamets ("Pair up with Fungi!") and the knowledge and helpfulness at The Shroomery. Concentrating on Gourmet and Medicinal Mushrooms: Reishi, Shiitake, Garden Giant, Lion's Mane, Turkey Tail.

Acronyms (for noobs like me): http://www.shroomery.org/forums/showflat.php/Number/5875173#Post5875173
Have opted out of rating system because of personal reasons, read my bio if you are interested in why. (I opted out before receiving or giving any ratings, meaning no offense to anyone.)

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