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mycomattie


Registered: 11/15/12
Posts: 1,323
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Psi. azurescens outdoor project for fall 2015 - as it progresses 4
#21119350 - 01/14/15 07:39 AM (10 years, 4 days ago) |
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12/3/14
I've missed my window of opportunity in the past, creating/preparing an outdoor patch for the following season. I started this in my journal section, but figured I'd try to make a grow log as it progresses - so I can get feedback and suggestions along the way 
Prints were acquired via trade - both were obviously wild collected specimens from the PNW.
This year I was finally able to start early, or at least on schedule. I inoculated both Psi azurescens and Psi cyanescens on MEA plates, but ended up keeping only the Psi azurescens going; since I was able to isolate some pretty gnarly looking sectors, while the Psi cyanescens germination and growth was "EH" at best.
That, and the fact that I have limited petri's - so I use them rather sparingly.
Initial inoculation was on 11/11/14, with the first signs of germination on 11/14/14

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How first transfer grew out as of 11/30/14

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12/02/14

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Made second transfer(s) today, 12/03/14
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12/6/14
 
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12/9/14
 
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12/14/14
 
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12/17/14
Trying out rye berries and alder chip quarts to inoculate with the Psi azurescens agar wedges.

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12/20/14
Psi azurescens is close to being in need of either another transfer, or used as an inoculant. I also inoculated quarts of rye berries/alder chips.
 
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12/24/14
Mycelium is colonizing the rye berries/alder chips well.
 
This weekend, I'm going to 'noc up a few jars and make a couple transfers from this plate.

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12/26/14
Pressure cooked 8 cup, PP5 plastic containers with the rye berry/alder chip mix - didn't work out too well, the plastic seemed to get soft and deform a bit; then cooled and inoculated with agar wedges.
Rye berries/Alder chips/Gypsum
 
After 1:45:00 pressure cooking at 15PSI and cooled, inoculated with agar wedges.

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12/28/14
A couple shots of the mycelium reaching an alder chip.
 
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12/29/14
I see more colonization in four days between 55° - 65°F, than I do in 14 days using other Psilocybe parameter incubation temps (75° - 80°F)

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12/31/14
Just a pic from the PP5 container - mycelium reaching.

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1/7/15
Quarts are about 90% colonized and PP5 containers are starting to takeoff. I will spawn to straight alder chips, and keep building the spawn amount until Spring.
  
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1/12/15
I'm liking these 6 quart, shoe box totes to get the spawn going.
    
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1/17/15
Azurescens mycelium is so thick and brilliantly white. It looks like the wood chips are getting covered by white frosting.
 
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1/22/15
Coming along very nice, I think I may spawn to a larger tub with more wood chips.
 
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1/24/15
Spawned to ~ 3 lbs of alder chips and card board.

1/27/15
At this rate - I wont be able to make an outdoor azurescens bed until May!

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1/28/15
The tote looks good (in my opinion?)
 
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1/30/15
I'm really hopeful with this Psi azurescens spawn - it's really tearing through the wood chips and cardboard. MUCH faster than my past attempts, maybe the agar/iso work paid off?
  
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2/1/15
Just a cool myc pic

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2/3/15
I have to keep feeding it wood chips, mycelium growth is aggressive - I hope it doesn't contam/stall (as mentioned above) as it keeps getting thicker, considering it seems like this winter will never end.
  
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2/11/15
Mycelium is still tearing through the woodchips - this tub has to weigh close to ten pounds at this point; I might have to consider splitting it up? Winter has NO end in sight here
   
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2/11/15
Added another Alder bag to the Psi azurescens tote. It's getting heavy, but I still have't broken it up - the mycelium looks healthy and smells great.
  
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3/2/15
Tub weights 14.9 lbs at this point and looks like I'll need to add more chips soon.
  
3/2/15
Snow is finally melting - 18 lbs of spawn has long been ready to go outdoors.
 
4/13/15
Finally made a few outdoor patches under rhododendron bushes bushes. The soil around the bushes consists of old, broken down mulch/wood chips from the previous years.
Here is a pic of one of the beds.

Edited by mycomattie (04/13/15 02:59 PM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie]
#21119400 - 01/14/15 08:01 AM (10 years, 4 days ago) |
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Oh man. You got it going on. Awesome job!
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: azur]
#21119527 - 01/14/15 08:55 AM (10 years, 4 days ago) |
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Thanks man - my first try so I'm hoping it goes OK
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Mr.PhilCybin
Master Baiter


Registered: 06/13/11
Posts: 11,646
Loc: Gnarnia
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie]
#21119546 - 01/14/15 08:59 AM (10 years, 4 days ago) |
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lookin good man.
I'd love to start a patch of these one day as well. I have a print waiting, just need the right conditions!
until then,
-------------------- I'm stupid, Falcon91Wolvrn03 is smart.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: Mr.PhilCybin]
#21119551 - 01/14/15 09:03 AM (10 years, 4 days ago) |
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Hell yea
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: wowimflabbergasted]
#21126332 - 01/15/15 06:46 PM (10 years, 3 days ago) |
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I'm just hoping I have the patience to take this all the way to the fall
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie]
#21126341 - 01/15/15 06:47 PM (10 years, 3 days ago) |
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Hell fuckin' yeah,
Along for the ride,
-------------------- Gyo's Better Grows
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: taGyo]
#21126349 - 01/15/15 06:49 PM (10 years, 3 days ago) |
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Thanks man - I appreciate the company haha
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PNWMusicMaker
Hunter



Registered: 10/18/14
Posts: 634
Loc: PNW
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie]
#21126424 - 01/15/15 07:00 PM (10 years, 3 days ago) |
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Nice looking work for sure. I'm doing something similar with Ps.Cyan in order to start some new outdoor patches and spread things around a bit. I like how much of that work I can do indoor. Not sure how picky people are but I've been buying condiment cups at almost any grocery store for like 3 bucks for a pack of fifty. Not quite as nice as an actual petri but they seem to work pretty well as a cheap ass option.
-------------------- Live to change, change to live.
  
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: PNWMusicMaker]
#21126439 - 01/15/15 07:04 PM (10 years, 3 days ago) |
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Quote:
PNWMusicMaker said: Nice looking work for sure. I'm doing something similar with Ps.Cyan in order to start some new outdoor patches and spread things around a bit. I like how much of that work I can do indoor. Not sure how picky people are but I've been buying condiment cups at almost any grocery store for like 3 bucks for a pack of fifty. Not quite as nice as an actual petri but they seem to work pretty well as a cheap ass option.
Thanks man! I'm jealous of people who live in an area where they can go out and get wild mycelium and prints - not much of anything here.
Excellent, are the condiment cups pre-sterilized (manufacturing process), or do you PC them (PP5)?
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PNWMusicMaker
Hunter



Registered: 10/18/14
Posts: 634
Loc: PNW
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie]
#21126456 - 01/15/15 07:09 PM (10 years, 3 days ago) |
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I haven't bothered to sterilize them and all of my contams I traced back to my own mistakes in handling things, lack of using an SAB, lack of prior experience and you know just being a noob for a few weeks. I'm doing some things right and a lot of things wrong just to get my feet wet so to speak.
I love the PNW even more now that I've gotten into hunting. I only need to pick Azzies and I'll have personally collected four of the WA Psilocybes. Makes fall something to look forward to that's for sure.
-------------------- Live to change, change to live.
  
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: PNWMusicMaker]
#21126474 - 01/15/15 07:13 PM (10 years, 3 days ago) |
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Quote:
PNWMusicMaker said: I haven't bothered to sterilize them and all of my contams I traced back to my own mistakes in handling things, lack of using an SAB, lack of prior experience and you know just being a noob for a few weeks. I'm doing some things right and a lot of things wrong just to get my feet wet so to speak.
I love the PNW even more now that I've gotten into hunting. I only need to pick Azzies and I'll have personally collected four of the WA Psilocybes. Makes fall something to look forward to that's for sure.
Right on, I'll have to check them out!
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie] 1
#21161393 - 01/22/15 07:51 AM (9 years, 11 months ago) |
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1/22/15
Coming along very nice, I think I may spawn to a larger tub with more wood chips.

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Mr.PhilCybin
Master Baiter


Registered: 06/13/11
Posts: 11,646
Loc: Gnarnia
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie]
#21161841 - 01/22/15 09:55 AM (9 years, 11 months ago) |
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looks good man
-------------------- I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.
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Dekozn
Stranger than kindness


Registered: 04/12/06
Posts: 1,183
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: Mr.PhilCybin]
#21165514 - 01/22/15 11:23 PM (9 years, 11 months ago) |
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 Love the OP, very clear and nice pics. Did you use any antibiotics for germinating the wild spores and if not were there any signs of bacteria or other contams? Also at which temp did you let the spores germinate on the plate? I can't grow cube now cause it's way to cold but my fingers are itching so maybe I could get away with psi. cyan.  Got a psi. cyan. print that I like to put on agar but thinking that maybe I should get some anti-biotic agar first for germinating the spores or would that be over-kill...
-------------------- Organized people are just to lazy to search for their stuff...
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: Dekozn]
#21165542 - 01/22/15 11:32 PM (9 years, 11 months ago) |
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AMU Q&A
If you don't have a plan of your own, you'll become a part of somebody else's.
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: Grey]
#21165613 - 01/22/15 11:57 PM (9 years, 11 months ago) |
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: d0urd3n]
#21166677 - 01/23/15 09:41 AM (9 years, 11 months ago) |
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Quote:
Dekozn said:
 Love the OP, very clear and nice pics. Did you use any antibiotics for germinating the wild spores and if not were there any signs of bacteria or other contams? Also at which temp did you let the spores germinate on the plate? I can't grow cube now cause it's way to cold but my fingers are itching so maybe I could get away with psi. cyan.  Got a psi. cyan. print that I like to put on agar but thinking that maybe I should get some anti-biotic agar first for germinating the spores or would that be over-kill...
thanks!
Nope, just MEA - just quickly transferred clean mycelium from the initial plate. The temps I germinated the spore on the agar was ~ 75°F, though I've noticed that the mycelium definitely grew faster at colder temps (65°F during the day and dropping down to 55°F at night).
Yeah, I just fruited cubensis (AA+) for the first time in the dead of winter, and I definitely need a casing layer in my climate during the winter - the heat blasting constantly dried out the substrate and I have ALL side pins, I'm really disappointed in how it turned out
I personally wouldn't bother with antibiotic agar, just let the spores grow out a bit when germinated and transfer any clean mycelium.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 71,179
Loc: The Inexpressible...
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: mycomattie]
#21166806 - 01/23/15 10:15 AM (9 years, 11 months ago) |
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Cant wait to see how this goes. A rare grow indeed!
-------------------- "The beginnings of unlimited energy will likely emerge around 2024 and will become a worldwide manifestation by 2040. Once unlimited energy is established, the possibilities will be endless - your progressive movement will accelerate to astronomical heights." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Psi. azurescens outdoor project for fall 2015 - as it progresses [Re: LogicaL Chaos]
#21171508 - 01/24/15 03:02 PM (9 years, 11 months ago) |
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1/24/15
Spawned to ~ 3 lbs of alder chips and card board.
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