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OfflineMarwhoa
Stranger

Registered: 12/04/14
Posts: 2
Last seen: 9 years, 2 months
First B+ Grow!
    #21108760 - 01/12/15 04:48 PM (9 years, 4 months ago)

Hey all,

I've been lurking on these forums since about the beginning of December, and after researching a little every day, I feel like I'm ready to start my first grow!

The idea is to prepare agar with Pastywhyte's Easy Agar Tek, do Agar-to-Agar transfers until I have a contaminant-free agar. I'll then inoculate with grains (WBS), do G2G transfers, and then eventually move onto a monotub. I have a spore syringe with B+ spores.

So, I prepared the agar and PCed it. The first round didn't turn out so well, I didn't know how to PC lol:



The second batch turned out better, although the Agar is kind of riding up the mini-rounds (is this ok?). So I went through sterile procedure (washed myself, cleaned room, etc) and put droplets of the spore syringe into the mini rounds in a SAB. I made sure to flame sterilize between each round.

Unfortunately, I don't have any parafilm, so I just placed the test rounds into a plastic baggie:

Any advice is much appreciated! :smile:

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OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 6 months
Re: First B+ Grow! [Re: Marwhoa]
    #21108775 - 01/12/15 04:52 PM (9 years, 4 months ago)

You don't need parafilm for the test rounds.

I just stack them on top of each other in my fridge if I'm not using them,

To prevent the agar from creeping up make sure you're starting off your PC run lower then hi. On my 10-heat stove I put it on 8 to get it to 15 PSI and then drop it to 7 once I get there.

If those are inoculated then take them out of the bag and set them wherever. They'll be fine,

I'll be watching,
:thumbup:

- Gyo


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum

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