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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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No problem! I learned it in this thread way back on like page 60 or something.
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DredgeMyEyes
Stranger


Registered: 11/05/14
Posts: 273
Last seen: 1 month, 23 days
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Quote:
Munchauzen said: No problem! I learned it in this thread way back on like page 60 or something.
Just checked out all the vids, thanks for those, seeing it in motion really helps alot. it will help to clear up all the doubt I'll have regarding procedures and technique, the same doubt I have every time I do something invasive in the SAB lol
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Quote:
PussyFart said:
Quote:
taGyo said: What contam is this:


Thats just a trippy picture of planet earth
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Edmunter]
#21103413 - 01/11/15 06:50 PM (9 years, 1 month ago) |
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What the heck is this? This was a transfer from a clone and long after it had colonized the whole plate, it started showing these dots? Mold?

I hate my phone for zooming in on this stuff. Sorry for the blur.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,404
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Quote:
stareatclouds said: What the heck is this? This was a transfer from a clone and long after it had colonized the whole plate, it started showing these dots? Mold?

I hate my phone for zooming in on this stuff. Sorry for the blur.
That looks like when I get bruising on a plate. How old is it? You said this showed up 'long after' it had colonized.
Could be drying out.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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I first made the transfer on 12/20, so about 3 weeks old. The little dots hadn't moved or spread or grown in the past week since I'd noticed it so I doubt it's actually mold since mold and bacteria grow pretty fast, right?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Looks bruised to me.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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How does myc like that become bruised without a contaminant falling onto it? I haven't touched it or even opened it since I inoculated.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,404
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Bruising isn't only caused by touching. Like I said, it could be drying out.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Oh, I must've missed that when I first read it. Right on. Thanks. Interesting little formation. I tossed it, not wanting to risk a contamination.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Quote:
PussyFart said:
Quote:
taGyo said: What contam is this:


Was never able to get a clear answer.
Never saw it before.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: taGyo]
#21103937 - 01/11/15 08:34 PM (9 years, 1 month ago) |
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Let's hope you never see it again.
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Pastywhyte's Easy Agar Tek [Re: taGyo]
#21105904 - 01/12/15 04:38 AM (9 years, 1 month ago) |
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Quote:
taGyo said: I love divided plates?
Not sure what's NOT to like about them? Pouring is just as easy as normal plates and I blew through a 100 pack of those and now am working on a 500 of tri. I can take three different samples and label them and use that instead of three petris. It's great for limited MS grows.
You are sure definately on top of things Gyo, for most of us , thats just hurry the fuck up!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Quote:
Imperfect Iam said:
Quote:
taGyo said: I love divided plates?
Not sure what's NOT to like about them? Pouring is just as easy as normal plates and I blew through a 100 pack of those and now am working on a 500 of tri. I can take three different samples and label them and use that instead of three petris. It's great for limited MS grows.
You are sure definately on top of things Gyo, for most of us , thats just hurry the fuck up!
I have a lot of shit planned. Just got my blender for slurry because A2G fucking sucks.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Pastywhyte's Easy Agar Tek [Re: taGyo]
#21106225 - 01/12/15 08:11 AM (9 years, 1 month ago) |
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u are the man! I should not speak of everyone, to ME, thats hurry the fuck up!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Quote:
Imperfect Iam said: u are the man! I should not speak of everyone, to ME, thats hurry the fuck up!
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pastywhyte's Easy Agar Tek [Re: taGyo]
#21106264 - 01/12/15 08:25 AM (9 years, 1 month ago) |
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I did A2G twice just to see if I could do it and man, it's sooo slow compared to LI. I'm glad I started with LI's instead of agar chunks.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Quote:
stareatclouds said: I did A2G twice just to see if I could do it and man, it's sooo slow compared to LI. I'm glad I started with LI's instead of agar chunks.
I dropped a wedge in some grains about......... 10 days ago.
It's got a small chunk colonized. I look over at Eat's fuckin' slurry thread and homeboy is boasting 6 day full colonization speeds.
Tf am I doing?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: taGyo]
#21106416 - 01/12/15 09:10 AM (9 years, 1 month ago) |
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A2G can be fast if you know how. Cut a big wedge to a pint of millet or WBS. Shake well, this allows for lots of spots for the myc to rub off onto the grain. In 4-5 days it should be at least 30-40% colonized. Shake it and its done 3 days later. G2G that pint to 6 quarts and then a week or two later its ready to spawn. Not quite as fast as LI but safer if your not confident with your sterile tek.
Also G2G might seem slow off the bat but if you have the time it can be expanded exponentially.
1 quart to 10 10 to 100 100 to 1000 1000 to 10000
If it takes a week for each G2G expansion and two to start the inital master thats 10000 quarts in 5 weeks. You would need a lot tubs to warrant that much spawn.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#21106444 - 01/12/15 09:17 AM (9 years, 1 month ago) |
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I must have been doing it wrong. For me A2G took longer than spores. By the time my A2G jars were colonised I had lost interest in the grow and started something else.
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