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OfflineDaBrownOne91
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Condesation inside agar mini round?
    #21102643 - 01/11/15 03:48 PM (9 years, 4 months ago)

I just completed my first agar attempt using PastyWhites awesome tek.  When I took my rounds out of the PC there was condensation on the walls. Is this normal?

I brought the pressure up very slowly and let the PC cool overnight.

I dropped some spores from a MS syringe anyways, but just seeing if this will lead to problems.

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InvisiblePastywhyteMDiscord
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Re: Condesation inside agar mini round? [Re: DaBrownOne91]
    #21102725 - 01/11/15 04:02 PM (9 years, 4 months ago)

It is normal for some condensation to be present. Will usually clear up in a couple days. Is not a contam vector and as long as you don't introduce bacteria thro poor procedure it will not be any issue. Good job getting into the best part of the hobby :thumbup:

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OfflineDaBrownOne91
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Re: Condesation inside agar mini round? [Re: Pastywhyte]
    #21102839 - 01/11/15 04:38 PM (9 years, 4 months ago)

Thanks!  I don't know why it took me so long.  The string of contams pushed me into it. 

How long until I start seeing growth with agar?

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Re: Condesation inside agar mini round? [Re: DaBrownOne91]
    #21102891 - 01/11/15 04:48 PM (9 years, 4 months ago)

From spores? Anywhere from 3 days to never depending on age and viability. When I do spores to plates I usually nock up 3 or 4 plates then transfer from the strongest fastest plate.

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Invisiblebudmanman
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Re: Condesation inside agar mini round? [Re: Pastywhyte]
    #21103191 - 01/11/15 05:59 PM (9 years, 4 months ago)

I just put some spores from a print taken on 2-2-2013 on agar. If they germinate is the dna in the myc still as good as when the spores were produced?

Edited for re wording.


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

Edited by budmanman (01/11/15 06:12 PM)

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Re: Condesation inside agar mini round? [Re: budmanman]
    #21103294 - 01/11/15 06:21 PM (9 years, 4 months ago)

Yes but the numbers are not as in your favor. I find that with older spores it can be harder to find strong growth. Tho this is not a solid rule, your experience may vary.

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Invisiblebudmanman
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Re: Condesation inside agar mini round? [Re: Pastywhyte]
    #21103331 - 01/11/15 06:33 PM (9 years, 4 months ago)

Ahh, have you ever taken a spore print from a mushroom grown from a old print and then found strong growth at that point?


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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Re: Condesation inside agar mini round? [Re: budmanman]
    #21103369 - 01/11/15 06:41 PM (9 years, 4 months ago)

Yes but its not as likely. It also typically takes a lot longer for germination. The spores are harder to rehydrate. I have had to go to stupid lengths to get some really old prints to even germ.

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Invisiblebudmanman
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Re: Condesation inside agar mini round? [Re: Pastywhyte]
    #21103388 - 01/11/15 06:44 PM (9 years, 4 months ago)

If they germ in 5 days do you think that would be a indicator they are pretty healthy?


--------------------
Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.

And I am mentally unstable.

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InvisibleGuardian187
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Re: Condesation inside agar mini round? [Re: budmanman]
    #21103398 - 01/11/15 06:47 PM (9 years, 4 months ago)

Pasty, when you say you're selecting the faster colonizing plate, are you just scraping off some mycelium to a new plate from the fastest colonizing sector of the plate, or are you actually transferring directly to your grains?

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OfflinePsilicon
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Re: Condesation inside agar mini round? [Re: Guardian187]
    #21103530 - 01/11/15 07:18 PM (9 years, 4 months ago)

Quote:

Guardian187 said:
Pasty, when you say you're selecting the faster colonizing plate, are you just scraping off some mycelium to a new plate from the fastest colonizing sector of the plate, or are you actually transferring directly to your grains?




Transferring from the fastest colonizing sector means taking a small wedge from that area where the growth is fastest and then putting it on another agar plate to keep that growth isolated from the other genetics in the original plate.  If that culture ends up fruiting well, you want to have it saved, right?  So best practice is to transfer to another agar plate, let it grow out, and then inoculate with a wedge from the new plate and then stick that new plate in the fridge for safekeeping.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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InvisibleGuardian187
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Re: Condesation inside agar mini round? [Re: Psilicon]
    #21103550 - 01/11/15 07:21 PM (9 years, 4 months ago)

Quote:

van der griegen said:
Quote:

Guardian187 said:
Pasty, when you say you're selecting the faster colonizing plate, are you just scraping off some mycelium to a new plate from the fastest colonizing sector of the plate, or are you actually transferring directly to your grains?




Transferring from the fastest colonizing sector means taking a small wedge from that area where the growth is fastest and then putting it on another agar plate to keep that growth isolated from the other genetics in the original plate.  If that culture ends up fruiting well, you want to have it saved, right?  So best practice is to transfer to another agar plate, let it grow out, and then inoculate with a wedge from the new plate and then stick that new plate in the fridge for safekeeping.




Ah ok, that makes sense.

So if you do isolate a fast colonizing sector and it fruits well and they're potent, would collecting a spore print be mundane?

Obviously, you'll want to have your isolated colony saved on some agar, but what if I wanted to collect a print to share or something if I got an awesome grow out of that isolation?

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OfflinePsilicon
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Re: Condesation inside agar mini round? [Re: Guardian187]
    #21103611 - 01/11/15 07:35 PM (9 years, 4 months ago)

Quote:

Guardian187 said:

So if you do isolate a fast colonizing sector and it fruits well and they're potent, would collecting a spore print be mundane?

Obviously, you'll want to have your isolated colony saved on some agar, but what if I wanted to collect a print to share or something if I got an awesome grow out of that isolation?




If you collect a spore print of a fast-growing, potent, good-fruiting clone or isolate, the genetics will be marginally better, but there's so much genetic diversity within the species it's still kind of a crap shoot.  You should always print as many of the mushrooms as you can bear to, IMO.  There are a few reasons:

1. You really want a backup in case you have to suddenly relocate, like I did.
2. Spores are valuable trade goods.
3. Spore giveaways are a good way to gather goodwill in the community, and generally are a decent thing to do.
4. It's good practice for if you ever go hunting and find a really great specimen that you want to print.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflineDaBrownOne91
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Re: Condesation inside agar mini round? [Re: Psilicon]
    #21105127 - 01/12/15 12:02 AM (9 years, 4 months ago)

Thanks again for taking the time to do this, Pasty.

My original plan was to make a bunch of plates and transfer one plate to one jar, instead of splitting a plate into many jars. Which method is better?

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Re: Condesation inside agar mini round? [Re: DaBrownOne91]
    #21105903 - 01/12/15 04:36 AM (9 years, 4 months ago)

Both are fine. One plate to one jar is better if your new to sterile work and will colonize much faster :super:

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InvisibleGuardian187
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Re: Condesation inside agar mini round? [Re: Psilicon]
    #21106136 - 01/12/15 07:26 AM (9 years, 4 months ago)

Quote:

van der griegen said:
Quote:

Guardian187 said:

So if you do isolate a fast colonizing sector and it fruits well and they're potent, would collecting a spore print be mundane?

Obviously, you'll want to have your isolated colony saved on some agar, but what if I wanted to collect a print to share or something if I got an awesome grow out of that isolation?




If you collect a spore print of a fast-growing, potent, good-fruiting clone or isolate, the genetics will be marginally better, but there's so much genetic diversity within the species it's still kind of a crap shoot.  You should always print as many of the mushrooms as you can bear to, IMO.  There are a few reasons:

1. You really want a backup in case you have to suddenly relocate, like I did.
2. Spores are valuable trade goods.
3. Spore giveaways are a good way to gather goodwill in the community, and generally are a decent thing to do.
4. It's good practice for if you ever go hunting and find a really great specimen that you want to print.




Great points. Cheers man!

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