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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#21087001 - 01/08/15 12:07 PM (9 years, 1 month ago) |
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I use a mix of petris and no pour in various sizes of PP5s. I honestly find the PP5s easier to work with than proper plates. With petries my fingers get a bit to close to the rime for my liking.
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aykaye47
Shroomerys Gangster


Registered: 03/19/14
Posts: 1,667
Last seen: 1 year, 3 months
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
#21091218 - 01/09/15 06:42 AM (9 years, 1 month ago) |
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What kind of food coloring do you guys use? i was reading the ingredients of some different kinds at my local grocery store and the gel has sugars in it and the liquid has preservatives in it. Im not sure but wouldnt preservatives hinder growth? Or would the small amount being used not make a difference?
-------------------- "Don't mistake my kindness for weakness. I am kind to everyone, but when someone is unkind to me, weak is not what you are going to remember about me"
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pastywhyte's Easy Agar Tek [Re: aykaye47]
#21092131 - 01/09/15 11:51 AM (9 years, 1 month ago) |
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It's probably better to use food coloring without but the coloring I use surely has those things as well, doesn't seem to have a negative effect as long as you don't use a lot of it.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
#21092163 - 01/09/15 11:59 AM (9 years, 1 month ago) |
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I bought $10 all natural food coloring at a farmers' market. stuff was shit. took way too much to color the agar. I've been using the Krogers/Kings Sooper brand since and it works fine.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
#21092174 - 01/09/15 12:00 PM (9 years, 1 month ago) |
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Yeah one drop of food coloring with sugar and weird shit is probably better than 10 drops of a different variety that sucks.
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
#21092319 - 01/09/15 12:42 PM (9 years, 1 month ago) |
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noc, one batch I tried to make black plates with the all natural stuff, which resulted in using way too much food coloring. the moisture content went off, and my plates remained a sludge-like consistency after the PC cycle. and it ended up like maroon not even black.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
#21092494 - 01/09/15 01:25 PM (9 years, 1 month ago) |
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I love divided plates?
Not sure what's NOT to like about them? Pouring is just as easy as normal plates and I blew through a 100 pack of those and now am working on a 500 of tri. I can take three different samples and label them and use that instead of three petris. It's great for limited MS grows.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
#21092504 - 01/09/15 01:26 PM (9 years, 1 month ago) |
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The stuff I use contains sugar and it has never caused any problems for me. I made black agar once. I had to use way too much coloring and the consistency was a bit off. It was black as night though.
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DredgeMyEyes
Stranger


Registered: 11/05/14
Posts: 273
Last seen: 1 month, 23 days
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
#21093297 - 01/09/15 04:31 PM (9 years, 1 month ago) |
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Quote:
MudaFuka said: The stuff I use contains sugar and it has never caused any problems for me. I made black agar once. I had to use way too much coloring and the consistency was a bit off. It was black as night though.
I considered the black food coloring but figured it would take a lot to get it dark so I *think* I grabbed red, McCormick brand...should be a'ight right?
Also, I have some LME, do you think I should utilize that since I have it or just stick with the flakes and all?
Thanks Bro'shroomites!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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If you have LME use it.
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DredgeMyEyes
Stranger


Registered: 11/05/14
Posts: 273
Last seen: 1 month, 23 days
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
#21093378 - 01/09/15 04:50 PM (9 years, 1 month ago) |
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Quote:
MudaFuka said: If you have LME use it.


Any fav recipe? haven't researched it yet but I do recall a 10mg agar with 10mg LME and 500 ml h2o
Also, my agar is very noodle like, anyone ever used this type? i'm pretty sure it's fine
and these injection ports are tiny oops
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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http://www.shroomery.org/forums/showflat.php/Number/18676888#18676888 Here are a few agar recipes. I've never seen that type of agar but give it a shot.
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DredgeMyEyes
Stranger


Registered: 11/05/14
Posts: 273
Last seen: 1 month, 23 days
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
#21093441 - 01/09/15 05:05 PM (9 years, 1 month ago) |
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Quote:
MudaFuka said: http://www.shroomery.org/forums/showflat.php/Number/18676888#18676888 Here are a few agar recipes. I've never seen that type of agar but give it a shot.
Thanks for the link, awesome thread, he put alot of effort into that agar testing so we don't have to. This is a great community of people, nobody is stingy with their info and methods and actually do things to help further it, good stuff.
ground crickets!? lol
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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What contam is this:
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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DredgeMyEyes
Stranger


Registered: 11/05/14
Posts: 273
Last seen: 1 month, 23 days
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
#21100869 - 01/11/15 10:13 AM (9 years, 1 month ago) |
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Made some plates using LME 2.5g Agar 2.5g and a drop of Karo with 120ml of water. seemed to turn out alright, condensation is covering the inside even though I treated the temperature/pressure very gingerly through the whole process, tried to bring it up real slow AND bring it down slow by decreasing the flame a little at a time for the last 2 or 3 minutes. let sit for hours + overnight.
since I only have the stuff I wanna work with in syringes, I added those tiny injection ports lol
Annoying, the stack fell over a little in the pc and a few of them tilted so the agar is very misshaped, still gave it a 'noc anyway for the hell of it.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: Pasty Agar Tek [Re: taGyo]
#21100896 - 01/11/15 10:19 AM (9 years, 1 month ago) |
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Quote:
taGyo said: What contam is this:

--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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I've recently adapted a new agar recipe as well. My ratio was 1:1.5:2, glucose:agar:potato flakes. Its firm and sticky, which works well for all my upside-down techniques with the pasty plates.
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DredgeMyEyes
Stranger


Registered: 11/05/14
Posts: 273
Last seen: 1 month, 23 days
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Quote:
Munchauzen said: I've recently adapted a new agar recipe as well. My ratio was 1:1.5:2, glucose:agar:potato flakes. Its firm and sticky, which works well for all my upside-down techniques with the pasty plates.
Upside down? are you throwin tissue samples at it like a velcro ceiling?
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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DredgeMyEyes
Stranger


Registered: 11/05/14
Posts: 273
Last seen: 1 month, 23 days
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Quote:
Munchauzen said: yes http://www.shroomery.org/forums/showflat.php/Number/21032261
Good stuff! Makes sense, now that I think about it lol
Bookmarking this for later on tonite
Thanks Munch'
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