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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Rye Soak Water Liquid Culture! [Re: Violet]
#21087114 - 01/08/15 12:29 PM (9 years, 4 months ago) |
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Indeed.
Quote:
hamloaf said: Researched with WBS, high quality Red Milo, and rye. Rye being the choice of grains to run spawn through for this cultivator. So many advantages to using rye. Never ran millet before. Almost did one year, but found rye instead. Seeing as how many commercial mushroom cultivators use millet, millet definitely peaks my interests. Just been fortunate enough to always have a source of rye readily available.
In fact, to stretch the rye supply, the Red Milo is primarily what's used in grainmaster jars. Said grainmasters containing colonized Red Milo are then transferred using grain to grain transfers into receiving bags of sterilized rye. Rye does best at at fruiting for two major reasons. 1. Size of grain. The larger the grain the more moisture it can hold and release at fruiting time. 2. Nutrition level of grain. Rye is the most nutritious of all grain choices we can use in this hobby. The more nutritious the medium is, the slightly slower the mycelium will take to colonize it which is almost always made up for at fruiting time.
Quote:
Violet said: Why do you think #2
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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13shrooms
Lightning Shaman


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Rye Soak Water Liquid Culture! [Re: Violet]
#21087142 - 01/08/15 12:35 PM (9 years, 4 months ago) |
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ever notice when blackdust, violet and azure are in a thread its never on topic. you all need to UTSF! 
Im done with the 3 of you 
the very next thread I see derailed by either of you youre getting banned from mushcult for a month.. test me 
now back on topic with Hams thread...
I believe this was the last on topic discussion
Quote:
hamloaf said: Researched with WBS, high quality Red Milo, and rye. Rye being the choice of grains to run spawn through for this cultivator. So many advantages to using rye. Never ran millet before. Almost did one year, but found rye instead. Seeing as how many commercial mushroom cultivators use millet, millet definitely peaks my interests. Just been fortunate enough to always have a source of rye readily available.
In fact, to stretch the rye supply, the Red Milo is primarily what's used in grainmaster jars. Said grainmasters containing colonized Red Milo are then transferred using grain to grain transfers into receiving bags of sterilized rye. Rye does best at at fruiting for two major reasons. 1. Size of grain. The larger the grain the more moisture it can hold and release at fruiting time. 2. Nutrition level of grain. Rye is the most nutritious of all grain choices we can use in this hobby. The more nutritious the medium is, the slightly slower the mycelium will take to colonize it which is almost always made up for at fruiting time.
Quote:
Violet said: Why do you think #2
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Rye Soak Water Liquid Culture! [Re: 13shrooms]
#21087179 - 01/08/15 12:40 PM (9 years, 4 months ago) |
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I didn't derail this thread. In case you haven't noticed, it hurts other members more than it hurts me when you guys ban me.
I agree with the moisture content of rye mentioned above and have said so many times. Rye ftw
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13shrooms
Lightning Shaman


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Rye Soak Water Liquid Culture! [Re: azur]
#21087211 - 01/08/15 12:45 PM (9 years, 4 months ago) |
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azur said: I didn't derail this thread. In case you haven't noticed, it hurts other members more than it hurts me when you guys ban me.
I agree with the moisture content of rye mentioned above and have said so many times. Rye ftw
Quote:
azur said:

you sure dont help it stay on topic and at Quote:
In case you haven't noticed, it hurts other members more than it hurts me when you guys ban me.

now quit talking about it and be about it.. stay on topic
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Rye Soak Water Liquid Culture! [Re: 13shrooms]
#21087320 - 01/08/15 01:03 PM (9 years, 4 months ago) |
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Log in to view attachment
Quote:
13shrooms said: ever notice when blackdust, violet and azure are in a thread its never on topic. you all need to UTSF! 
Im done with the 3 of you 
the very next thread I see derailed by either of you youre getting banned from mushcult for a month.. test me 
---- Anyway, Here is "Hamloaf's Mushroom Cultivation Archive" download. If you want me to add more teks to the pdf then just message me Ham. That is, if I dont get banned by 13shrooms.
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: Rye Soak Water Liquid Culture! [Re: blackdust] 1
#21090175 - 01/08/15 10:21 PM (9 years, 4 months ago) |
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Quote:
Violet said: Why do you think #2
You said: "#2" 
Nah, for real though. For one, the more food that's available to the mycelium during culturing (the colonization of culturing media such as agar, liquid media, and master culture slants), colonization (grain spawn running), and spawn run (colonization of the bulk substrate) causes the mycelium to NOT have to reach out so fast in search of food, hence the slightly slower colonization rate of highly nutritious media.
Second, a mushroom's food source is the nutrient laden moisture that evaporates from the substrate. The higher the nutritional content of the substrate, the more nutrients are in the evaporated moisture coming from the substrate, the more nutrients that laden the moisture evaporating from the substrate, the more food is made available for the fruit bodies to absorb resulting in stronger, healthier the fruits.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21090359 - 01/08/15 11:13 PM (9 years, 4 months ago) |
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hell yeah man. LC's Rock the shit!
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13shrooms
Lightning Shaman


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21090508 - 01/09/15 12:01 AM (9 years, 4 months ago) |
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Quote:
hamloaf said: the more food that's available to the mycelium during culturing (the colonization of culturing media such as agar, liquid media, and master culture slants), colonization (grain spawn running), and spawn run (colonization of the bulk substrate) causes the mycelium to NOT have to reach out so fast in search of food, hence the slightly slower colonization rate of highly nutritious media.
Second, a mushroom's food source is the nutrient laden moisture that evaporates from the substrate. The higher the nutritional content of the substrate, the more nutrients are in the evaporated moisture coming from the substrate, the more nutrients that laden the moisture evaporating from the substrate, the more food is made available for the fruit bodies to absorb resulting in stronger, healthier the fruits.
--------------------
A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: Rye Soak Water Liquid Culture! [Re: 13shrooms]
#21101984 - 01/11/15 01:41 PM (9 years, 4 months ago) |
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Full colonization of the grain substrates has been reached as of about yesterday.

Figured since the idea of this particular grain water liquid culture was spawned by PastyWhyte, and I that it was deemed appropriate to spawn this grain spawn into 27 quart, minimono tubs, Pasty's favorite style of fruiting chamber. This is a great opportunity to develop research of these tubs, simultaneously.
The minimonos are purchased and modified into fruiting chambers.

Haven't decided yet how much spawn and bulk that's going to be used per tub, or if there will be various substrate depths experimented with. Most likely the varying substrate depth option. The substrate will be comprised of horse manure/coir/vermiculite/gypsum. Maybe some spent coffee grounds. We'll see.
The diluted high quality Red Milo liquid medium is colonizing right away. Barely see through, colonization can be observed, as well as being able to see clearly through the murky medium, so long as you focus.

Can't even BEGIN to see through the non diluted culturing media, so not sure if any thing is going on in there, but hope isn't abandoned on this culture yet..

There is a lot of LC projects going on at once.

LC MANIA!
More to come.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,867
Loc: Canada
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf] 1
#21102073 - 01/11/15 01:59 PM (9 years, 4 months ago) |
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Beautiful ham, those grains look great 
Ya got some really nice projects going on, thanks for sharing
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21102280 - 01/11/15 02:42 PM (9 years, 4 months ago) |
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Quote:
hamloaf said:
Quote:
Violet said: Why do you think #2
You said: "#2" 
Nah, for real though. For one, the more food that's available to the mycelium during culturing (the colonization of culturing media such as agar, liquid media, and master culture slants), colonization (grain spawn running), and spawn run (colonization of the bulk substrate) causes the mycelium to NOT have to reach out so fast in search of food, hence the slightly slower colonization rate of highly nutritious media.
Second, a mushroom's food source is the nutrient laden moisture that evaporates from the substrate. The higher the nutritional content of the substrate, the more nutrients are in the evaporated moisture coming from the substrate, the more nutrients that laden the moisture evaporating from the substrate, the more food is made available for the fruit bodies to absorb resulting in stronger, healthier the fruits.
Duty 
My question was specifically referring to this:
Quote:
hamloaf said: 2. Nutrition level of grain. Rye is the most nutritious of all grain choices we can use in this hobby.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Guardian187
Neophyte


Registered: 11/29/14
Posts: 716
Loc: Center of the Universe
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Re: Rye Soak Water Liquid Culture! [Re: Violet]
#21103686 - 01/11/15 07:50 PM (9 years, 4 months ago) |
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Sweet writeup! LOL at the comments
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Rye Soak Water Liquid Culture! [Re: Violet]
#21124684 - 01/15/15 11:33 AM (9 years, 4 months ago) |
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Also Hamloaf I did this the other day, thought you might find it revealing as to the potential nutritive content of grainwater http://www.shroomery.org/forums/showflat.php/Number/21119913#21119913
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Rye Soak Water Liquid Culture! [Re: Violet]
#21124814 - 01/15/15 12:15 PM (9 years, 4 months ago) |
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
#21125218 - 01/15/15 02:12 PM (9 years, 4 months ago) |
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Quote:
d0urd3n said:

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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Guardian187
Neophyte


Registered: 11/29/14
Posts: 716
Loc: Center of the Universe
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Re: Rye Soak Water Liquid Culture! [Re: Grey]
#21125276 - 01/15/15 02:26 PM (9 years, 4 months ago) |
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Is bacillus common with grain grows? How would you even identify that versus colonizing mycelium?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 7 months
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Re: Rye Soak Water Liquid Culture! [Re: Guardian187]
#21125332 - 01/15/15 02:46 PM (9 years, 4 months ago) |
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Quote:
Guardian187 said: Is bacillus common with grain grows? How would you even identify that versus colonizing mycelium?
More common then most people know.
Normally a wet spot against the grain. It's also called Wet Spot contam. If you search you'll find plenty of pictures or look in my log. You'll see fully colonized grains with a bunch of uncolonized spots where the grains are pressed against the glass.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
#21125919 - 01/15/15 05:05 PM (9 years, 4 months ago) |
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Ham, What should I be looking for in the LC? I have something growing in the bottom of my jar. Looks white and fluffy. I'm sure they are contamed. I knocked up with MS 10 days ago.
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: Rye Soak Water Liquid Culture! [Re: blackdust]
#21126446 - 01/15/15 07:07 PM (9 years, 4 months ago) |
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White and fluffy is good. Let's see it (pics?). More of your questions may be gone over here as The Visual Signs Of A Contaminated Liquid Culture are discussed in further detail.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21126473 - 01/15/15 07:13 PM (9 years, 4 months ago) |
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I will get some up. I also did not dilute the grain water. Something is growing for sure.
Here is a pic after a few days. Do you see those white flakes on top? weird.

The flakes have not grown but their is something growing on the bottom of the jar.
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