Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblemary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
Trusted Cultivator
New culture from year old dried shrooms?
    #21083754 - 01/07/15 06:36 PM (9 years, 4 months ago)

I have some year old dried shrooms stored under vacuum in my freezer. I'm curious, can I take a cutting from one of these and sterilize it & hydrate it; would it grow if I put it on an agar plate?

Sorry if this question has been asked before- I'm new to the site and this is my first post.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: New culture from year old dried shrooms? [Re: mary fairchild]
    #21083901 - 01/07/15 07:02 PM (9 years, 4 months ago)

if you sterilize it you'll kill it but they're already dead and that won't work

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: New culture from year old dried shrooms? [Re: bodhisatta]
    #21083991 - 01/07/15 07:22 PM (9 years, 4 months ago)

You can grow from a dried mushroom, but I doubt a year old frozen mushroom kept in anaerobic conditions would still be viable.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: New culture from year old dried shrooms? [Re: krypto2000]
    #21084052 - 01/07/15 07:35 PM (9 years, 4 months ago)

if that mushroom was dried in a dehydrator it's dead 140F kills mycelium dead but 165F is about where the dehydrators operate. 165F is far from too hot to destroy actives though hence why a food dehydrator is the best way to dry shrooms unless you have 4K to spare on a freeze dryer

Extras: Filter Print Post Top
OfflineThe Librarian
The Accountant
Male


Registered: 01/03/15
Posts: 393
Loc: Hiding under a Mushroom
Last seen: 8 years, 10 months
Re: New culture from year old dried shrooms? [Re: mary fairchild]
    #21084060 - 01/07/15 07:36 PM (9 years, 4 months ago)

Just get some spores and start fresh.


--------------------
Spore prints or it didn't happen.           Consolidate is a fancy word for: "If you touch it some more any time soon you'll kill it all."                              Great Scorpions Song
       

Extras: Filter Print Post Top
OfflineNecNomen
Stranger
 User Gallery
Registered: 10/15/13
Posts: 12
Last seen: 8 years, 10 months
Re: New culture from year old dried shrooms? [Re: mary fairchild]
    #21084148 - 01/07/15 07:58 PM (9 years, 4 months ago)

Yes, absolutely worth trying! I'll share my experience.

I had a tissue that I got from a friend after we weren't able to get any more. We wanted to be able to keep it around for a few of us close friends. The sample was a couple years old and as such was not sealed. It was just a random bit from the bottom of a large bag that had been in and out of the freezer several times. It was dry and I'm sure that's important.

I took the sample containing a couple of stems and did a LOT of reading on culturing and fungi. In the mean time I took two small jars of distilled water and put two tissues samples in them. The samples were taken under as clean of conditions as I could manage, from the center of the stems. My idea was to rehydrate the cells without providing nutrients for the growth of other organisms. You will need to be very good at aseptic technique. Also think through ahead of time how you will avoid contamination from the outside of your sample or your tools.

It took a few weeks if I remember correctly but I eventually saw what looked like a small amount of growth. I'm talking very small, remember there was no nutrients. It was kind of a fuzz or halo. I transferred very small pieces to several agar plates, about 2-3 dozen if I remember correctly. Actually I filled several of those plates with pieces of the dry sample as well. Everything very clean and careful. Many failed I think the only ones that were at all promising were from the hydrated tissue. All were contaminated in some way, but that's to be expected.

From there you'll need to do several transfers to isolate your mycelium from contaminates. It took a long time before the strain was capable of vigorous growth (weeks) and I didn't get good rizomorphic growth for several months after it had been grown on a few different types of medium. Once it's growing though, no matter how slow or contaminated your bound to succeed in time.

I got most the information from reading the forums. I'm sure mostly from Roger Rabbit. It was fun, thankfully, as it was also slow and expensive, lol.

PS. I had another fungus that seemed to run symbiotically with it for some time and wasn't easy to differentiate except by the ...texture?... of the mycelium. It just looked like there was two types of mycelium and it was very subtle. Never figured out how that happened, but I have theories so I warn that you may see the same.

Good luck!

Extras: Filter Print Post Top
OfflineNecNomen
Stranger
 User Gallery
Registered: 10/15/13
Posts: 12
Last seen: 8 years, 10 months
Re: New culture from year old dried shrooms? [Re: NecNomen]
    #21084153 - 01/07/15 08:00 PM (9 years, 4 months ago)

It's worth it to avoid spores, since you know the sample you have is something you want. And to repeat the above advice, don't sterilize!

Extras: Filter Print Post Top
Offline99.99
Stranger
 User Gallery

Registered: 12/22/14
Posts: 2,022
Last seen: 2 hours, 36 minutes
Re: New culture from year old dried shrooms? [Re: NecNomen]
    #21084247 - 01/07/15 08:20 PM (9 years, 4 months ago)

Grab spore from an unopened cap.... I've got them to grow easy

Extras: Filter Print Post Top
InvisibleGrey
⇜ ✯ ⇝
 User Gallery


Registered: 11/06/14
Posts: 6,223
Trusted Cultivator
Re: New culture from year old dried shrooms? [Re: 99.99]
    #21084258 - 01/07/15 08:23 PM (9 years, 4 months ago)

I would still get some spores, just in case.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.

Extras: Filter Print Post Top
Invisiblemary fairchild
Pantheist


Registered: 12/06/14
Posts: 777
Trusted Cultivator
Re: New culture from year old dried shrooms? [Re: 99.99]
    #21084306 - 01/07/15 08:33 PM (9 years, 4 months ago)

Thanks for your responses!

The shrooms were dried over several days at room temperature using desiccant. I'm waiting on some spores right now, but while I wait,

I think I'll give it a try. If it doesn't work there is no great loss. I was thinking of dipping a cut piece of shroom in some 3% peroxide, then (in my glove box) trying to hydrate it in sterile water- then just plop it on to an agar plate and see what happens.

Thanks NEC NOMEN!
I'll consider your experience with this, I'll probably get some contaminants along the way. I'll do it with and without peroxide treatment.

mf

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* Re: Question about dried shrooms Vorpal 938 2 06/10/00 10:24 AM
by kg
* Re: Drying shrooms GoaTrip 4,563 6 04/22/01 10:51 AM
by slither
* best way to dry shrooms philistine4life 9,693 4 10/16/01 01:16 AM
by philistine4life
* smell of dried shrooms? wind_song 4,304 5 09/15/01 07:44 PM
by Abudiwa
* Color of dried shrooms? SlimDanny 7,785 2 04/03/02 02:33 PM
by Canisdormit
* newbie question- viable spores from dried shrooms? mr crisper 2,673 5 05/14/02 05:18 AM
by ChromeCrow
* Over Dried Shroom? TaliesinIII 3,647 5 08/03/01 08:00 PM
by wind_wraith
* drying shrooms - too dry? farmer_maggot 1,999 3 10/16/01 05:07 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
698 topic views. 23 members, 125 guests and 50 web crawlers are browsing this forum.
Calendar Event: 01/07/55
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.