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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21273251 - 02/14/15 01:12 PM (9 years, 10 months ago) |
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Which is why that's an unfair comparison. G2G should be done in sterile air.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: AGAR'S GRAIN LC [Re: Psilicon]
#21273258 - 02/14/15 01:14 PM (9 years, 10 months ago) |
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Quote:
van der griegen said: Which is why that's an unfair comparison. G2G should be done in sterile air.
still makes GLC safer imo. Dont you see all these people with SABS getting bacteria and mold in their tubs.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21273270 - 02/14/15 01:17 PM (9 years, 10 months ago) |
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No, not that many. Usually it's still air bathrooms.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: AGAR'S GRAIN LC [Re: Psilicon]
#21273276 - 02/14/15 01:18 PM (9 years, 10 months ago) |
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Do G2G in a SAB and watch your success rate improve. Its not the fault of the method if you choose to implement it incorrectly. Again I am not bashing GLC but there is a reason commercial operations do not expand with that method. Most use G2G or LI to expand. FrankHorrigan experienced his biggest contam meltdown with GLC. I'm sure most people who are familiar with him agree he knows his stuff. If he can lose 24 tubs with it anyone can. Understand the pitfalls. GLC will work great until it doesn't. The biggest risk with liquids especially GLC is that when it does let you down, it lets you down big.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Considering that 1 master jar of GLC can produce 100x as much as 1 G2G master jar and I can test out a GLC and know if its good or not within a week is the reason it has my love. Not to mention that I dont have to work in a little plastic box. Testing out a GLC before MASSIVE expansion is like following up with a MASSIVE institution ~ Gotta do it or deal with the risk of getten fucked.
Is this not what agar is for? Talk about it enough. lol
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21273339 - 02/14/15 01:38 PM (9 years, 10 months ago) |
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That is what agar is for. If your inoculating your master with agar and testing it with agar then you have little to worry about. But if I was using agar I would just inoculate LC and remove the extra stick of the SHIP or the risk of germinated endospores. Takes less time to colonize an LC with a nice agar wedge than it does to colonize a grain master, if speed is your concern.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21273341 - 02/14/15 01:39 PM (9 years, 10 months ago) |
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Quote:
blackdust said: I can test out a GLC and know if its good or not within a week is the reason it has my love.
Are you saying that you can take a glc, inoculate more jars, spawn those jars, and get fruits within a week?
Or are you saying you test it out on agar for contams first?
Considering that PCing doesn't kill everything on grains, I hope you test that GLC regularly if you store it.
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Edited by SpitballJedi (02/14/15 01:41 PM)
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blackdust


Registered: 02/28/09
Posts: 8,327
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Quote:
SpitballJedi said:
Or are you saying you test it out on agar for contams first?
Considering that PCing doesn't kill everything on grains, I hope you test that GLC regularly if you store it.
Yes. I actually tested my fist GLC on cakes as shown in the OP. I would recommend testing on agar before using to not repeat what happened to Frank. Or test on a small batch of cakes and then slurry the cakes to grain jars. 1 cake can make 50 grain jars. I want to try this GLC again. Now that I know Eat can fully colonize a PF cake in 4 days I want to try the same with a GLC instead of steam tissue.
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Yerow
Stranger



Registered: 09/22/14
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21273492 - 02/14/15 02:25 PM (9 years, 10 months ago) |
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Think it would help/do anything to let that water sit in the jar for a few hours, maybe shake a few times in that time, to really get that myc out into it?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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back from the dead. good to see you bd.
hey sbj been a long time man how ya been?
oh guess what i just made sbj?
i made all my tub hole magnets! colonizing 4 tubs now with the magnets on and then ill put the Dilated filters on next during fruiting. i did 4 tubs with 8 quarts of spawn to 11 quarts of substrate. sub depth was about 4". going to case in about 3 days when the subs finish colonizing. i haven't been able to beat my 3 day bulk colonization average yet. but im trying hard. i don't think it can get any faster. i did make some slight modifications. i would usually spawn my tubs with the polyfil in the holes and put the tub in a draft free area. now ive started using the magnets to colonize the tubs in my grow room with the fan moving air. then switch up to poly and dilated filters when fruiting. the issues i was having for the last several months i have worked out. which was directly related to how tight i was stuffing my bottom polyfil holes. i had them in, but not solid bulging tight. i made that minor change, built a new grow setup with a small fan and its working rock solid now. i also started using a 6500 light rather than natural daylight. the ceiling fan wasn't cooperating lol. the lighting isn't making much of a difference other than the fruits grow directly toward it rather than all over the tub with diffused light. yields still the same in cased and uncased bulks, natural diffused light or artificial light. doing about 6-9z dry average per tub. ill pm ya the magnet pics.
Quote:
SpitballJedi said:
Are you saying that you can take a glc, inoculate more jars, spawn those jars, and get fruits within a week?
GLC takes me about 7-9 days for full colonization in quart grain jars. for grain slurry to bulk=2 weeks from inoculation to harvest. 12-13 days on average for slurry. but a GLC takes me about 5 extra days to colonize quarts. so for GLC 18 days till fruit from inoculation of grain jars to harvest when your spawning to bulk tubs. if you fruit directly from bottles that would decrease the time by a few days up to a week.
i don't use a SAB for g2g or any of that and i get near 100% success without contamination. i don't think the method matters. if its a flowhoood, shmuv, sab, its your technique and following the principle using that method that ensures success. ive used all 3 and the contam rate is the same.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Hey eat, good to see you. It has been a while. Glad to see you're still kicking ass. 
The time scale you mention seems reasonable.
Like PW mentioned, why make GLC instead of Agar LC? We've talked enough to know this is an honest question. You've inspired me to question my methods in the past and I've actually changed some things to make it easier because of it.
-------------------- The Basics
A little civility goes a long way
The Noob Forum
The Hammock Hangers' Forum
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Quote:
SpitballJedi said: Hey eat, good to see you. It has been a while. Glad to see you're still kicking ass. 
The time scale you mention seems reasonable.
Like PW mentioned, why make GLC instead of Agar LC? We've talked enough to know this is an honest question. You've inspired me to question my methods in the past and I've actually changed some things to make it easier because of it.
your not really making a GLC. its almost already there before you. you simply blend up the grain in the water and you have a very large volume of LI for inoculation. i guess you could say for convenience. ill give an example, i g2ged 10 quart jars. thats all i have going(maybe you have a situation where your growth stalled or life got in the way). i want to spawn 2 tubs and need a quick turnaround to get more spawn going for another round of tub spawning and flushes. i could 1)g2g one last quart and make 10 more quarts for spawn. or i could keep that 1 grain jar and make enough inoculant for over 100 quart jars. its easy because you already have a large volume of culture that you can transfer. you could use a plate. but it all really depends on the situation. if your using an LI to inoculate a bunch of grain jars, use that last grain jar for a GLC. and then you spawn the other 9 jars. then that one GLC turned into enough spawn for 20 tubs or more.
1 its easy. and if you dont' have any agar plates or anything of that nature you can use your last remaining grain jar as a GLC to transfer and make a ton more jars.
2. that one grain jar can inoculate enough jars comparable to 10 or more plates of LI. you could also use a grain from that remaining quart and put it on agar. but then you would need to grow that out on a plate. when you already have a large volume of inoculum at your disposal. the way i look at it is, if you hav a quart volume of liquid inoculant. you have an unlimited amount of spawn that you can make in a moments notice, and its convenient and not that difficult.
Edited by eatyualive (02/14/15 09:28 PM)
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Neomorph



Registered: 12/10/14
Posts: 767
Loc: Europe
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21278349 - 02/15/15 03:17 PM (9 years, 10 months ago) |
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Nice work dude, the jar has only water or it has honey to ?
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: AGAR'S GRAIN LC [Re: Neomorph]
#21278352 - 02/15/15 03:18 PM (9 years, 10 months ago) |
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Quote:
kastriotr said: Nice work dude, the jar has only water or it has honey to ?
distilled water that has been pressure cooked along with the syringe.
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21313151 - 02/22/15 01:40 PM (9 years, 10 months ago) |
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After completing this do you toss the grain in the jar or will it recover? I am about to try this out.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: AGAR'S GRAIN LC [Re: blazedup]
#21313248 - 02/22/15 01:48 PM (9 years, 10 months ago) |
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let the jar recover.
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
Last seen: 4 months, 15 days
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Re: AGAR'S GRAIN LC [Re: blackdust]
#21313275 - 02/22/15 01:54 PM (9 years, 10 months ago) |
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Quote:
blackdust said: let the jar recover.
Thanks
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TrIpPyDuDe
FUCK


Registered: 12/07/14
Posts: 1,263
Loc: Kentucky
Last seen: 9 years, 9 months
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Re: AGAR'S GRAIN LC [Re: blazedup]
#21313311 - 02/22/15 02:03 PM (9 years, 10 months ago) |
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I just bought pre poured agar plates. Can't wait to use them
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