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OfflineThe_LosT_SouL
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Registered: 03/22/03
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Last seen: 17 years, 2 days
Mycelium Transfere from Jar to Spawnbag
    #2108228 - 11/15/03 03:00 AM (17 years, 5 months ago)

Hypothetical Situation:

One has a 5L bag 1/2 full of sterylized BRF(or other) substrate ready for innoculation.

One also has a 250ml full colonized jar of P.cubensis mycelium.

One then births the jar and slices of a slice of the colonized substrate, places this newly sliced slice into the bag of what one might call "SPAWN".

One then places this bag in a recently sterylized garbage bag and ties up, or in a chamber designed to have clean air in there basically all of the time.

Do you think this bag of substrate would colonize? Maybe a cold shock would be in order.

If this bag did colonize, would it be very good to use in a casing.

If anyone has an answere or idea's about this. Let me know


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


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InvisibleautomanM
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Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108271 - 11/15/03 03:24 AM (17 years, 5 months ago)

you can inoculat a bag with a jar. i recommend not using brf, though. rye or wbs would be better. a 1:8 spanw to bag ratio would be best. meaning that 250mL should knock up 2L in a bag. be sure to be sterile in the transfers. dont worry about slicing the cake up. once it is sealed in the bag, you can break it up.


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No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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OfflineThe_LosT_SouL
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Re: Mycelium Transfere from Jar to Spawnbag [Re: automan]
    #2108310 - 11/15/03 03:43 AM (17 years, 5 months ago)

Thanks for info automan.

So I should use 1 jar per 2L or 2 jars per 4L...

What is WBS

Can i use "rye" and vermiculite like i do with BRF
Anyspecial kind of rye?
Do you know what bags would work best?
Do you think we could sterilize alot of substrate in a big ball of tinfoil? could that work?


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


Edited by The_LosT_SouL (11/15/03 03:43 AM)


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InvisibleautomanM
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Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108332 - 11/15/03 03:58 AM (17 years, 5 months ago)

wbs = wild bird seed.

on the rest: http://www.fungifun.org/grain/


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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OfflineThe_LosT_SouL
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Re: Mycelium Transfere from Jar to Spawnbag [Re: automan]
    #2108339 - 11/15/03 04:00 AM (17 years, 5 months ago)

Thanks automan
Maybe i'll keep you posted on my results after I find a pressure cooker :smile:


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


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OfflineThe_LosT_SouL
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Registered: 03/22/03
Posts: 83
Last seen: 17 years, 2 days
Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108348 - 11/15/03 04:04 AM (17 years, 5 months ago)

Should I use the rye for the the jar that will innoc the bag? and should I use the rye in the big bag of substrate?


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


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InvisibleautomanM
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Registered: 09/18/03
Posts: 8,271
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Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108383 - 11/15/03 04:28 AM (17 years, 5 months ago)

i'd say yes to both of those


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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OfflineThe_LosT_SouL
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Registered: 03/22/03
Posts: 83
Last seen: 17 years, 2 days
Re: Mycelium Transfere from Jar to Spawnbag [Re: automan]
    #2118854 - 11/18/03 03:15 AM (17 years, 5 months ago)

Do I want my substrate to be loose or brickey?
Where might I find those bags used in vaccum packaging?


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds, Feminized Cannabis Seeds, High THC Strains, USA West Coast Strains   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Amazon ½ Pint Jars, Brown Rice Flour, Hot Plate, Pressure Cooker, Rye Grain, Vermiculite, Wild Bird Seed

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