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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Rye Grain, Vermiculite, Wild Bird Seed

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OfflineThe_LosT_SouL
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Registered: 03/22/03
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Last seen: 13 years, 2 months
Mycelium Transfere from Jar to Spawnbag
    #2108228 - 11/15/03 03:00 AM (13 years, 8 months ago)

Hypothetical Situation:

One has a 5L bag 1/2 full of sterylized BRF(or other) substrate ready for innoculation.

One also has a 250ml full colonized jar of P.cubensis mycelium.

One then births the jar and slices of a slice of the colonized substrate, places this newly sliced slice into the bag of what one might call "SPAWN".

One then places this bag in a recently sterylized garbage bag and ties up, or in a chamber designed to have clean air in there basically all of the time.

Do you think this bag of substrate would colonize? Maybe a cold shock would be in order.

If this bag did colonize, would it be very good to use in a casing.

If anyone has an answere or idea's about this. Let me know


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


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InvisibleautomanM
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Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108271 - 11/15/03 03:24 AM (13 years, 8 months ago)

you can inoculat a bag with a jar. i recommend not using brf, though. rye or wbs would be better. a 1:8 spanw to bag ratio would be best. meaning that 250mL should knock up 2L in a bag. be sure to be sterile in the transfers. dont worry about slicing the cake up. once it is sealed in the bag, you can break it up.


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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OfflineThe_LosT_SouL
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Re: Mycelium Transfere from Jar to Spawnbag [Re: automan]
    #2108310 - 11/15/03 03:43 AM (13 years, 8 months ago)

Thanks for info automan.

So I should use 1 jar per 2L or 2 jars per 4L...

What is WBS

Can i use "rye" and vermiculite like i do with BRF
Anyspecial kind of rye?
Do you know what bags would work best?
Do you think we could sterilize alot of substrate in a big ball of tinfoil? could that work?


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


Edited by The_LosT_SouL (11/15/03 03:43 AM)


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InvisibleautomanM
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Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108332 - 11/15/03 03:58 AM (13 years, 8 months ago)

wbs = wild bird seed.

on the rest: http://www.fungifun.org/grain/


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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OfflineThe_LosT_SouL
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Re: Mycelium Transfere from Jar to Spawnbag [Re: automan]
    #2108339 - 11/15/03 04:00 AM (13 years, 8 months ago)

Thanks automan
Maybe i'll keep you posted on my results after I find a pressure cooker :smile:


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


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OfflineThe_LosT_SouL
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Posts: 83
Last seen: 13 years, 2 months
Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108348 - 11/15/03 04:04 AM (13 years, 8 months ago)

Should I use the rye for the the jar that will innoc the bag? and should I use the rye in the big bag of substrate?


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleautomanM
blasted chipmunk
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Registered: 09/18/03
Posts: 8,009
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Re: Mycelium Transfere from Jar to Spawnbag [Re: The_LosT_SouL]
    #2108383 - 11/15/03 04:28 AM (13 years, 8 months ago)

i'd say yes to both of those


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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OfflineThe_LosT_SouL
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Registered: 03/22/03
Posts: 83
Last seen: 13 years, 2 months
Re: Mycelium Transfere from Jar to Spawnbag [Re: automan]
    #2118854 - 11/18/03 03:15 AM (13 years, 8 months ago)

Do I want my substrate to be loose or brickey?
Where might I find those bags used in vaccum packaging?


--------------------
The light dimmed, as I further, helplessly staggered down the cold stoned ramp, into the stagnent depths of the Ananaki Catacombs. |The walls are covered in Mushrooms.. Gulp, chomp, glump! mmmmm goood!


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Rye Grain, Vermiculite, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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