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OfflineFreeWorldOrder
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Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake.
    #21081854 - 01/07/15 10:40 AM (9 years, 4 months ago)

Made some PE6 jars since I had a bunch of extra spores.

Someone gave me a few 1 pint widemouth jars they didn't want, so I wanted to see how a BRF cake would colonize in a larger jar...

So far it seems to be going great with lots of rhizo and is not far behind the 1/2pt jars.

I should have made more this size so I could get a comparison between 1pt jars, but it looks as these could work out pretty decent.




--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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PastyWhyte's Easy Agar TEK
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Invisibleblackdust
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: FreeWorldOrder] * 1
    #21082000 - 01/07/15 11:20 AM (9 years, 4 months ago)


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OfflineFreeWorldOrder
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: blackdust]
    #21082054 - 01/07/15 11:39 AM (9 years, 4 months ago)

Right on... seems like they would fruit pretty good!


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK

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OfflinecronicrFacebook
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: FreeWorldOrder]
    #21082285 - 01/07/15 12:55 PM (9 years, 4 months ago)

2 half pints will out perform a pint in both colonizing times and performance 90% of the time IME. helps to take a little water out of the recipe as well but gl!


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Invisibleblackdust
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: cronicr]
    #21082298 - 01/07/15 01:01 PM (9 years, 4 months ago)

Quote:

cronicr said:
2 half pints will out perform a pint in both colonizing times and performance 90% of the time IME. helps to take a little water out of the recipe as well but gl!




This is true. Thats why I posted the qt cake. :facepalm:

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OfflinecronicrFacebook
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: blackdust]
    #21082322 - 01/07/15 01:10 PM (9 years, 4 months ago)

i did a couple of qt cakes a few years ago that looked stellar,took forever and had went anaerobic in the center but put out a flush from hell:lol:


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InvisibleGuardian187
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: cronicr]
    #21082327 - 01/07/15 01:12 PM (9 years, 4 months ago)

haha at the sequence of images :smile:

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Invisibleblackdust
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: cronicr]
    #21082331 - 01/07/15 01:13 PM (9 years, 4 months ago)

I guess you were smart enough to not use a glass jar. :facepalm:

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OfflinecronicrFacebook
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: blackdust]
    #21082342 - 01/07/15 01:17 PM (9 years, 4 months ago)

Quote:

blackdust said:
I guess you were smart enough to not use a glass jar. :facepalm:



no i used glass but i have a couple cases of tapered qt's i use for truffles:thumbup:


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InvisibleGuardian187
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: cronicr]
    #21082348 - 01/07/15 01:18 PM (9 years, 4 months ago)

Quote:

cronicr said:
Quote:

blackdust said:
I guess you were smart enough to not use a glass jar. :facepalm:



no i used glass but i have a couple cases of tapered qt's i use for truffles:thumbup:




Will quart wide mouth jars not work as well for truffles? I'm about to start some and was planning to use these jars.

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OfflinecronicrFacebook
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: Guardian187]
    #21082361 - 01/07/15 01:22 PM (9 years, 4 months ago)

no they work great for them i just like the look of sliding it out as a cake:lol:


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Invisibleblackdust
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: cronicr]
    #21082373 - 01/07/15 01:25 PM (9 years, 4 months ago)

Quote:

cronicr said:
no they work great for them i just like the look of sliding it out as a cake:lol:





This is true. I have made over 100 using the qt jars. Yummmm :thumbup:

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InvisibleGuardian187
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: blackdust]
    #21082391 - 01/07/15 01:29 PM (9 years, 4 months ago)

haha nice. I can't wait! Man I am such a noob compared to you guys heh. Enjoying the hell outta all this tho :smile:

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InvisibleGuardian187
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: Guardian187]
    #21082402 - 01/07/15 01:32 PM (9 years, 4 months ago)

And your signature Blackdust

:greatjob: x10

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OfflineKizzle
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: cronicr]
    #21082909 - 01/07/15 03:39 PM (9 years, 4 months ago)

If you can get them to colonize go for it. I would enjoy seeing a comparison. A lot of people have run into problems with stalling or just slow colonization particularly on the bottoms.


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Invisibleblackdust
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: Kizzle]
    #21083090 - 01/07/15 04:13 PM (9 years, 4 months ago)

Quote:

Kizzle said:
If you can get them to colonize go for it. I would enjoy seeing a comparison. A lot of people have run into problems with stalling or just slow colonization particularly on the bottoms.






Yup. Last part to colonize.




http://www.shroomery.org/forums/showflat.php/Number/11671929

The cake took 40 days to full colonize with consolidating for two week. It may have performed better if I gave it better conditions as Cron was talking about how he has done some of these with quote "results from hell". :lol:

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OfflineKizzle
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: blackdust]
    #21083127 - 01/07/15 04:20 PM (9 years, 4 months ago)

Quote:

blackdust said:
Quote:

Kizzle said:
If you can get them to colonize go for it. I would enjoy seeing a comparison. A lot of people have run into problems with stalling or just slow colonization particularly on the bottoms.






Yup. Last part to colonize.




http://www.shroomery.org/forums/showflat.php/Number/11671929

The cake took 40 days to full colonize with consolidating for two week. It may have performed better if I gave it better conditions as Cron was talking about how he has done some of these with quote "results from hell". :lol:



Was that with spores or LC?


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Invisibleblackdust
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: Kizzle]
    #21083166 - 01/07/15 04:30 PM (9 years, 4 months ago)

I was testing out Agar's LC tek for the first time. As you can see in my earlier post where I was dunking the cakes that their are 12 half-pint, regular mouth PF cakes + the qt cake. So I was able to get 100% success rate with this method on my first try. May not be as fast as other methods but better than MS IMO. Slurry seems to be my new favorite method now though for expanding large amounts of grain spawn in breath taking speeds. I would like to add that I did Agar's tek in open air, taking advantage of the SHIP in the jars. I went on to use his method for at least a hundred other jars with no contams. In open air for any new member who may be reading this. The SHIP's provide an easy way for beginners to inoculate jars and create LC with ease. I know many other members may disagree but I have to question if they have even used SHIP's before and are just trying to promote their own methods and discrediting others without trying them.

Quote:

agar said:

Pint jar on right is multispore inoculated (SA).
Allowed to colonize near 100%.
Syringe is 60 ML w/18 gauge needle.
Quart jar on left is sterilized water.

Aspirated 60 ML sterile water into syringe.

Injected 60 ML sterile water into colonized grain jar.

Shook grain jar to liberate mycelium from grain.

Injected total of 180 ML sterile water into grain jar.
(shaking mildly between each injection)

Aspirated out mycelium laden water from grain jar.
(all I could aspirate out of the grain jar was 150 ML)
Then, injected mycelium laden water back into sterile water jar.

Approximately 500 ML of Mycelium laden water. :rockon:
(enough LC for approximately 50....10 ML syringes)

The advantage of this procedure is that you can be assured.
The culture in clean.:grin:
Because you can VISUALLY inspect colonization in the grain jar.
(if there is FUNK, you can see it :rolleyes:)

That is not the case, with sugar type LC's.
(multispore syringe inoculated)
They can be contaminated, from day 1.
And, you NEVER know it. :frown:
Until you inoculate spawn jars with that LC.
Then, have every single spawn jar GO SOUTH. :eek: :mad2:



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OfflineKizzle
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: blackdust]
    #21083362 - 01/07/15 05:25 PM (9 years, 4 months ago)

So is slurry just another name for a GLC? Or by slurry you mean it came from PF cake instead of grains?


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Invisibleblackdust
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Re: Tried 1 pint wide mouth jar to see how MS would colonize a BRF cake. [Re: Kizzle] * 1
    #21083545 - 01/07/15 05:58 PM (9 years, 4 months ago)

Kizzle, where have you been? :lol: Eatyualive is getting whole 66qt monot tubs colonized in 3 days with two qts of WBS grain slurry. This is big! Granted, yields may be affected with the low grain ratio but I think their may be around this to still yield the 9 - 12 oz on first flush as if using 7 - 10 qts of spawn. I still have much testing to do for the workaround before I start telling people to do this for the same yields but things look promising. I should know for sure with a couple months but the speed is breath taking.

Look:

Quote:

eatyualive said:
just to show something that will throw many on a loop. this is a 2 quart wbs grain slurry to 9 quarts of verm. this is day 10. 3rd try on the slurries. subs were oven pasteurized. this is uncased. the sub colonized in 3 days and was put into fruiting on day 4.

Substrate is 9 quarts volume.

1 small brick coir
2 quarts verm
a cup of gypsum
5 quarts water

its usually 10 quarts volume for me. what i did was take about a quart of that volume out and use it as casing layers for other tubs.








I'm sure you have seen his slurry thread. Viewers, here it is.

SLURRY

Quote:

eatyualive said:
Liquid Spawning or Inoculant 2014:

First of all, TV Casualty is the man who invented this. This has revolutionized mushroom cultivation and everyone needs to know that you can successfully have fully colonized quart jars in 3 days.

TV Casualty's Slurry In a Hurry Tek

Results: 3 Days to colonize quart jars. Tv Casualty's results



My results: Day 1, Day 2, 77 hours, 82 Hours, 89 hours, 101 hours, later with a very weaker than normal slurry.



This is a comparison shot of the 10 day old P2G(PF to grain transfer) method side by side with the 4 day old slurry jars. The p2g has the red mag lite next to it.




Spawn Bag made with the same F+ slurry batch after 82 hours(2pics), 89 hours, 101 hours.



1 PF half pint jar used to inoculate 30 Quarts





tombosley8 results:




Friend's Spawn Bag Results; Day 3-5



Bag Spawn after 3 days:




WARNING FOR USING THIS TEK: NOT FOR NEWBIES

This tek is more of a step 4 process after you have learned some of the basic principles of mushroom cultivation. I suggest using it after you have mastered some of the principles below first.

1. learn basic sterile/clean principles
2. get a few tub grows in or have a grasp on growing and the entire mushroom cycle even if in pf jars or bag grows.
3. learn the basics of properly preparing grains. be able to successfully use grain spawn and grow out substrates successfully.have a grasp on bulk substrate and moisture content.
4. try liquid inoculants/ slurries when you have the first few principles down first.

PROCEDURE FOR JAR INOCULANT


Materials:
  • small mouth pint jar
  • band for the small mouth jar
  • foil
  • oster blender base and blade assembly
  • pressure cooked butter knife wrapped in foil
  • black plastic base for the blender attachment
  • one colonized 1/2 pint pf jar
  • as many colonized grain jars as you want up to 100: wbs, rye berry, popcorn
  • optional: you can also use this on grain prepared in filter patch bags
  • pressure cooked foil large enough to set a pf cake on. this is wrapped in foil

Reasoning:

Usually this method works well when you have about 20-100 quart jars you want to colonize in 3 days.  If you are only doing 10 quart jars then you wouldn't need to use about 90% of the slurry because you only need a small amount per jar. I will then use another method like P2G for fewer jars. However, if your using a smaller quart jar number you can easily add less water to the mix and make the slurry more muddy. This will reduce the risk of adding too much water to each jar. You only need a small amount per jar. I would compare this to the equivalent of about a tablespoon of slurry per jar. You also need to be careful that if you add too little of water, the cake won't properly blend in the jar. I would say 1/3 to one half of the pint jar is a good ratio. 1/3 on the muddy side, 1/2 on the more liquid side with more jars.

Procedure:

1. Prepare your utensils in the pressure cooker. I wrap a piece of foil large enough for me to flip a pf cake upside down on and cut it in half. I then wrap that piece of foil with foil and pressure cook it with other utensils.

2. As you can see the small mouth pint fits snugly to the bottom of the blade assembly. Fill the pint jar halfway with distilled water and place the blade assembly and ring on top of the assembly. Twist the ring so it is tighter but not too tight. Then place foil over that. Now it is ready for the pressure cooker. Some people have issues with the plastic grey gasket that hangs on the blade assembly. Mine is so old that it pretty much sticks to the blade assembly and i don't have to worry. However, you can also RTV silicone this directly to the lid in order to make things easier. You want to make sure that the bottom part that is touching the jar is not siliconed. Only silicone the top  to the blade assembly.




wide mouth jar att achment from tom!

Quote:

taGyo said:
So...
A blender for the wide mouths?



Quote:

tombosley8 said:
THIS JUST IN FOLKs:!!!!

Studies have found that there should be a mass recall on all blender attachment conversions on widemouth plastic lids (what i posted earlier:facepalm:) IT is apparent that there is a slow drip that could be detrimental to the health of our babies across the world. The Solution was revealed earlier today by a team of top researchers. They said not to be in fear as the metal lids do in fact create a safe seal and that any further commencement involving altering lids in this way should be done in a metal fashion. That concludes that our pesky and threatening drip is no longer at large and we may all return safely to our residences and be assured it will all be ok.




optional pour lid idea

Quote:

cArcace-x said:
ill probably use one of these PP5 lids, i just gonna make the hole a bit bigger, 1/2" like it's said in the thread 






3. Place your butter knife wrapped in foil along with your other utensils(pint jar with assembly) in the pressure cooker. Pressure cook for 30 minutes at 15psi. Let it cool. Then you can begin doing the clean work to do the liquid transfer.

4. Now that you have all your utensils sterilized and your grain jars prepared. It is now time to setup your clean work area. In my case I use a
Shmuvbox or you can use a floowhood or SAB. I setup all my jars to the far right out of the way so I have room to place all my utensils up front in the shmuv. This is so I can easily reach the utensils. I wiped all the jars down with rubbing alcohol prior to sticking them inside the clean work area. You can see my foil wrapped foil in the middle, my foil wrapped butter knife in the middle and the fully colonized pf half pint on the right. You will also notice the black assembly that attaches to the blade assembly for the base of the oster blender. I wiped this down with a bleach wipe prior to putting it in the shmuv.



5. You also want to setup your blender close by. I wipe the whole thing down from top to bottom with bleach wipes and even spray it with lysol.



6. Now you can prepare everything to ensure a quick transfer. This process involves taking off the foil and band lids to the pint jar or jars. You will also take the lid and foil off of your colonized pf jar. I place the items to the side out of my working area. But you can also take them out of the shmuv completely. Although if you remove your hands, always clean them thoroughly before placing them back in.



7. Now I am about to dump the pf cake upside down on my sterile foil.



8. Unwrap your butter knife and cut the pf cake in half. Now I am doing this because im trying a less slurry or muddy mix to see if it still works. You can simply use one pf cake and dump it upside down into the pint jar. This is a technique view of the 1/2 pint jar flipped upside down on the pint jar so that the cake slides right into the jar without touching anything. If you have to move the blade assembly be sure to set it face up not touching anything on the blade. You can hold it in your hand and flip the jar at the same time but you need to be careful not to touch it on anything. You don't want to introduce any contaminants into the jar.



9. Now if your using one pf cake for less jars. Simply flip the jar upside down shown in the previous step. Or if your making multiple jars for liquid spawn or liquid inoculant you can use your sterile knife to cut the pf cake in half. You have placed this pf cake on the sterile foil which you just unfolded. Work clean and fast. You then spear the side of the cake with the knife, open up the blade assembly touching only the outside and place the cake into the jar. You can position the cake so that you can simply use the cake edge against the inside edge of the jar to use as leverage to pull the knife out and the cake will drop right in. This takes about 3 seconds max.



10. Now I place my black blender attachment assembly on the top of the metal blade assembly. I clean the area so that when I am about to do the transfers, there is clear room to work clean and fast.



11. I now take the jar and assembly out of the shmuvbox but still in my cleaned work area and use the blender to blend the cake into slurry.



12. Now the slurry is ready to pour into each Jar or bag.

13. You simply bring your jar back into your work area. You have all your quart jars and bags with loose lids ready for transfer.

14. With one hand slightly lift up the blade assembly lid with your fore finger and you will then open the quart jar lid with your right hand. You pour about 1 tablespoon of slurry into each jar. The key is to quickly close both lids simultaneously after you do the quick transfer. This minimizes contamination.

15. After you have inoculated all of your jars and bags. You simply shake the jars for 20 seconds each. You want to distribute that extra water you poured into your grain jars as much as possible so that you don't have any issues after this. At first you will get a little scared because your jars will look like there is pooling water at the bottom once they have sat on the shelf a few hours. Be patient, wait it out. The idea is to have fully colonized jars in 3 days without a shake and it works.

FOR THE SLURRY IN A HURRY TEK DO NOT SHAKE THE JARS. THE KEY HERE IS TO HAVE FULLY COLONIZED JARS/BAG SPAWN IN 3 DAYS WITHOUT SHAKING THE JARS AT ALL.!!!!


BELOW ARE A FEW EXAMPLES OF MULTISPORE PF AMAZONIAN GROWS FROM GRAIN INOCULATED WITH SLURRY. THE GRAIN SPAWN WAS THEN SPAWNED TO A BULK SUBSTRATE. THE SUBSTRATE WAS NOT CONSOLIDATED AT ALL.


PROCEDURE FOR LIQUID SPAWNING BULK SUBSTRATES

Reasoning: So the idea here is to skip the grain spawn all together. and go straight from pf cake blended into liquid and use anywhere from 2 pints to 2 quarts of liquid spawn to mix into your substrate. 2 pints is quite a bit of liquid spawn. and you don't want to overdo it. so it might be ideal to do this with pints. if you have a few tubs to spawn. maybe quarts are not a bad idea for multiple tubs.

Moisture Addition and not overdoing your moisture content
you will have to prep your substrate as you normally do prior to this liquid spawning. however, there is one catch here. you will have to account for moisture addition from the slurry.

so you will want to make your substrate mix slightly drier than normal. you can do this by adding some dry verm at the end after you have mixed your substrate to field capacity. or some dry horse poo can soak up more of that moisture. so right now this is just an experiment. the ratios may change. but im going to mix my normal 2 turkey tins volume of substrate per tub. im going to try 1pf cake to spawn one tub of substrate. one tub is 2 turkey tins by volume of substrate.


Recipe of Substrate by volume for: 1 66 quart tub or similar size tub

2 turkey tins volume of pasteurized substrate  per 66 quart tub(16 quarts substrate total)

TAKE NOTE HERE: OUR SPAWN RATIO IS AMAZINGLY LOW HERE. IT IS 1/2 PINT SPAWN TO 16 QUARTS OF SUBSTRATE VOLUME. SO USE A GOOD COMPARISON IF YOU PLAN TO USE MORE LIQUID INOCULANT


The Slurry Per Tub is

1 half pint colonized PF  cake
for ease using small mouth half pint jars make dropping the cake into the pint jar easier. if you have a widemouth 1/2 pint you will have to cut the cake in half to fit it in your small mouth pint.

1 small mouth quart jar filled with 1 pint full of distilled water
(pced for 20 mins at 15psi prior)
(if you don't have a small mouth quart jar. and you only have 2 pint jars you can use these as well. you will have to cut the cake in half then blend it in each separate pint jar. this will be the same ratio just split in half. this will require 2 blade assemblies.  im finding that less water equals more sludge consistency of the cake. so lowering your water will make it a thicker mixture. the substrate catches this better.)

illustration of small mouth pint jars and quart jars with blade assembly ready for Pressure Cooker . 20 mins at 15psi.



picture of a turkey tin below




Substrate volume per tub:
1 small brick coir

6 cups of strawnet (it is boiled in coffee and water to add nitrogen. this isn't necessary just a trial for now. it is then strained out. no extra water is added just the water present in the strawnet)

2 quarts dry horse nugs

2 quarts dry vermiculite

1/4th cup supercake formula is placed in this as well

this should fill two turkey tins by volume of substrate so you can oven pasteurize. if you don't oven pasteurize then figure out a way to squeeze out the excess moisture from your substrate or add more dry vermiculite prior to spawning to account for adding the slurry liquid. or in the end after spawning you can just squeeze out the excess moisture once you mix in the slurry.

but you have to take into account. i mix my sub to over field capacity then oven pasteurize. this loses moisture due to evaporation. so being pasteurized over field capacity allows the subs to be at field capacity once pasteurization is complete. this becomes a science at times. its easy to misjudge. if you liquid or steam pasteurize and add water after or use water to pasteurize. you have to take into account the moisture content you need has to be a little dry to account for additional liquid.

also any substrate should work using this method. im just using the ratios i prefer. just to demonstrate here. just set your sub depth and mix your substrate volume accordingly. wouldn't suggest trying this with substrates such as straw or coffee for the time being until it has more trials.

Discussion
this is a trial experiment now. so the ratios are going to change. i will be using 2 pints of slurry liquid to my substrate for this trial run. the more volume of water you have blended the faster im assuming your spawn run will be. as you will have more blended mycelia touching more areas on the substrate.

the question is will the lack of nutrients from grain spawn decrease yield?


Procedure:
1. setup your clean area. i use just a plain and simple trash bag. the top is taped to a lamp with tape. and everything clean is placed in it. you then use disinfectant spray to clean everything. close the bag. i then cut holes for my hands to do clean work.  you can see below all the PF  jars and prepped sterile water in each small mouth pint and quart respectively. this also illustrates the plastic bag for semi clean work. since this is essentially like open air spawning. just be clean. not sterile.

   

2.loosen up your pf cake in your pf jar by lightly shaking the jar or tapping the sides of the jar. this will make it easy when you open up the pf cake and plop it into your quart. it should slide right out.

very simply open up your PF  jar and scrape off the dry verm with a sterile fork  or you can leave it on with no problems . then just open up your small mouth quart jar of water and plop the cake right in. try to align the tops of the jars so that the cake falls smoothly into the quart jar.



here is what it looks like after the PF  cake has been flipped and dropped into the quart jar. the black blender assembly is placed over the blade assembly.



remember im using small mouth half pints and small mouth pints and quarts. and you never touch anything on the outside of the jar. close the lid with the blade assembly. then place the black fitting for your oster and blend this jar up.

3. i have two blade assemblies. so its easy to do this with two jars. you may have to get another blade assembly from oster. or if you have some old blenders that happen to be oster that broke. detach the black part and the blade assembly. you blend the jars until the cake is liquefied. it may look slightly gooey. but this is ok. it will be a mycelia sludge. the sludgier the better. because when spawned it sticks to the substrate. it should look like this when your done. see where im heading with this. liquid inoculate, but ghetto version!



more illustrations of how a less water slurry will look

 

4. once your substrate is ready. place the plastic bag in your tub. then dump your pasteurized and cooled substrate into your bin and close the lid.

       
5. unscrew the black assembly from your jars. then just pour the jars into your substrate evenly all around.

 

6. thoroughly mix your slurry into your substrate. just like spawning you want to let the material touch every part of the substrate you can. and remember the substrate is actually drier than normal so the addition of this extra moisture should factor in perfect to your moisture content. if its slightly too moist. just squeeze it out in your clean area. you may have to mix up the substrate a bit to get the moisture to distribute more evenly but it shouldn't be that bad.

   

the idea is speed. if it works like it does in quart jars. then the substrate should colonize in a few days. this is perfect for if your having spawn issues from contamination. it skips the grain step all together.

and this is all done open air. just clean. you can blend and prep your slurry in a shmuvbox or trashbag, or flowhood. up to you. just have your clean area setup prior to doing all of this. i skipped details about clean work thats on you.


Notes:



Spawn Day 7-10-09
ok i found a bit of trouble upon doing the spawning. the 1 quart total of liquid i used i felt was far too little to the amount of substrate . i mixed it in as thoroughly as possible but i guess thats what the experiment is for. id say double it. 2 quarts of liquid would be perfect. and you will have to make your sub even drier. but i found my sub absorbed the moisture easily and was still adequete for field capacity.

basically the PF  jar was cut in half inside a plastic trash bag. then placed in each pint. the pints were blended. then the box was opened and the slurry was dumped in and spread evenly. then mixed together with gloves on.

spraying the sludge would probably work better. i think its better to even have less water and more sludge if that makes sense. more of a muddier consistency. so maybe even adding less water may work. the water will jolt down to the bottom while the muddy sludge stays at the top of the substrate.

but for large subs like this it might be ideal to try 2 PF  cakes worth of mycelia  to get this right. normally im using 4 to 5 quarts of grain spawn to this size sub.

i just feel it deep down that the slurry wasn't touching the entire sub and im gonna have a half fuzzy white sub with a half uncolonized sub lol. only experimentation will tell.


Day 2 Notes: 7-12-09
well growth is showing. it began the next day after spawning. now its looking like it was spawned with grain spawn  with small spots. ill get some pics up once it gets colonized more.

id say lowering your water content and making the slurry like mud with about 2 PF  cakes would work better. im going to attempt this next go round.. may try it with less substrate  as well. this last batch was probably about 3 turkey tins full just stuffed and compacted into 2 turkey tins. pics up soon...

but imagine the potential this has if one pf cake can slurry your substrate per tub. that would mean. no more quart mason jars necessary.

also remember this is one pf cake.



Day 3 Notes: 7-13-09

So if this tub does well and colonizes evenly in a decent amount of time. 2 more trials will be tested.

1. lower amount of water per PF  cake with less substrate  material

2. higher amount of water content per PF  cake with less substrate  material.

i feel the lower amount of water will make the PF  slurry like a sludge or very muddy. this seems to catch onto the substrate  better and not drop directly to the bottom. even when you do slurry to grain jars. you have that dripping effect. so to minimize this it might be better to try lower moisture in the slurry your adding. this might make it easier to keep your substrate  closer to field capacity  when this happens.

Day 4 Notes: 7-14-09
update. 4 days in. looking like a normal speed 4 to 5 quart sub spawning. interesting! it was looking a little fuzzy yesterday and i was worried it was cobweb . but apparently the mycelia  was growing from underneath the surface. i may merge some of these posts to let you see the progression side by side.
one observation. not nearly as much condensation on the sides of the chamber during spawn  run.
not to fear. the green color is strawnet.

Day 5 Notes: 7-15-09 
from day 4 til day 5 there is a dramatic difference in growth.

Day 6 Notes: 7-16-09
the substrate is almost done. will be birthed on day 7. one pf cake of spawn used. took a little longer than expected. but for 1 pf cake not bad. ideally we want 3 day spawn runs using this tek. so a few more experiments will have to be done to figure out what the perfect ratio is as far as the volume of slurry goes.

Day 7 Notes: 7-17-09
Birthday! The substrate was completely done about mid way through day 6. it was then birthed at 3am on 17th. the mycelia fluffed up really nice after growth in only one day. seems like everyrhing done this trial worked out perfectly. the rhizos are everywhere. for this trial run. not bad. however, we want a 3 day spawn run like the jars. so since there was about 1 pf 1/2 pint cake worth of mycelia to about 3 turkey tins of substrate. this in turn ended up taking a bit longer. i think if it is truly 2 turkey tins volume of substrate the spawn run should be half the time it took for this trial run.

Day 11 Notes: 7-21-09
Pins! If you can't tell top middle right of the picture. missed some of the more mature pins.

Day 18 Notes: 7-28-09
harvest! fruits got a bit stringy probably due to higher co2 levels. bins had to be taken out of ideal conditions when visitors arrived. last 3 days were in the dark with little to no airflow.

Conclusion and Notes:
so far in two tests the average colonization time for 1 pf jar of slurry is 6 days. the 2nd tub is on schedule to be colonized and birthed by day 7.

from spawn till harvest was 18 days. pinning took 4 days with a strain that was well over a year old. off 3rd generation clone material 2nd flush.

the fruits were not as thick as usual. the yield so far appears to be a little less than normal. seems to be about 4z dry from eye judgement. but for 1 pf cake of spawn material. thats nice!

the fruits were just as dense as the regular fruits of this strain comparatively. more experiments will be done. life interrupted this experiment a bit and the chamber had to be taken out of ideal conditions for the last 3 days of fruiting.


Side by Side Comparison

       
       
             


Day 2 
 

Day 4 


Day 5[/u
       

Day 6


Day 7
                     
   
Day 7




Day 11 
   
                     
Day 12 

                         
Day 13 
                   

Day 16                   


Day 17                         


Day 17


Day 18                       
       
       
         



Day 24: 2nd Flush
   



anyway pvacant has tested it with pans. and said he had a flush. im still waiting on pics for it. but here is what he said.

Quote:

ok eats, or anyone else who cares,

i got mature fruits on sunday (08/02), off of the pan cyan slurry in a hurry tek (i think it was 2-3 days in the fruiting  chamber). nothing great, about 1/4 of it flushed... BUT, this morning, i couldn't even count the amount of pins, (and Hyph knots) comming up......i have a pic, but i'm having hella computer problems, and it's taking about 5 minutes to do ANYTHING!, so if i can get this fixed i'll upload the pix. however the goal was to see if this tek will work with pans, and the answer is YES. i would however add, that because the myc is rather thin, compared to cubies, the water ratio should be ......perhaps (IMO)......1/2 pint BRF  - 1/2 pint H2O , or a 1:1 ratio of slurry. none the less Eats, you created a very easy way to extend pan myc, and obviously cubie myc, without grains! My vote is a YES! thanx








This method can be used in a wide range of applications. Look, I can expand 1 PF cake to a bag holding over 20 PF cakes worth of material in 6 days and I'm sure I could have spread that slurry to more spawn if I desired.



expanding the cake can be used to create more spawn material. More PF spawn material can be used to spawn to bulk and get tubs like this. All without a PC.  :super:


This is a bulk tray with 2 cakes:



I think some people without a PC may see the potential here.



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