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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Guardian187]
    #21063046 - 01/03/15 07:28 PM (9 years, 4 months ago)

Quote:

Guardian187 said:
Aside from being able to isolate cultures and identify any contams early (and without wasting sub) is there an immediate benefit to doing agar versus continuing G2G transfers?




The best part is that you don't burn through spore syringes. You can get hundreds of agar plates from a single spore syringe instead of a few grain jars.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisibleGuardian187
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Registered: 11/29/14
Posts: 716
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: NumeroEno]
    #21063097 - 01/03/15 07:34 PM (9 years, 4 months ago)

Quote:

EnoRoxxx said:
Quote:

Guardian187 said:
Aside from being able to isolate cultures and identify any contams early (and without wasting sub) is there an immediate benefit to doing agar versus continuing G2G transfers?




The best part is that you don't burn through spore syringes. You can get hundreds of agar plates from a single spore syringe instead of a few grain jars.




Ahh, nice! I was planning to scrape some prints I collect onto agar and go from there, but getting more juice out of a syringe would be nice :smile:

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InvisibleNumeroEno
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Guardian187]
    #21063136 - 01/03/15 07:42 PM (9 years, 4 months ago)

Quote:

Guardian187 said:
Quote:

EnoRoxxx said:
Quote:

Guardian187 said:
Aside from being able to isolate cultures and identify any contams early (and without wasting sub) is there an immediate benefit to doing agar versus continuing G2G transfers?




The best part is that you don't burn through spore syringes. You can get hundreds of agar plates from a single spore syringe instead of a few grain jars.




Ahh, nice! I was planning to scrape some prints I collect onto agar and go from there, but getting more juice out of a syringe would be nice :smile:




Agar is totally worth it. You can get set up with everything you need to work with agar for under 50 bucks if you already have a PC. A few months ago I was a noob and totally intimidated by the idea of working with agar. All I can say is that it's cheap, easy, and totally worth it.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: NumeroEno]
    #21063729 - 01/03/15 09:37 PM (9 years, 4 months ago)

whats the best way to do a mini mono and area sufficent for bulk? cost efficient SAB? is the a for sure no fail gtg? gtg seems to be the most efficient way atm because no clue how to agar. I've read several teks on all of them just don't know which to go with. new to all this ratio stuff, only used brf cakes in shotgun tubs before. thanks for all the suggestions.

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Invisiblejboredone
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21063758 - 01/03/15 09:43 PM (9 years, 4 months ago)

http://www.shroomery.org/forums/showflat.php/Number/21032606 pasty's mini mono tek.....just a nice size tube with 2 holes for your arms for the sab.....:jah:


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In order to grow old and wise, you must once have been young and dumb!

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: jboredone]
    #21068461 - 01/04/15 09:32 PM (9 years, 4 months ago)

When doing gtg do you need any special lids for the jars you are transferring to? or can you just leave them with the plain jar lids...

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Invisiblejboredone
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Posts: 4,798
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21068466 - 01/04/15 09:34 PM (9 years, 4 months ago)

regular lids with some kind of ge filter will be fine....:awethumb:


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Peace Pot Micro-Dot God Loves You High or Not!!!
In order to grow old and wise, you must once have been young and dumb!

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Invisiblejboredone
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: jboredone]
    #21068474 - 01/04/15 09:35 PM (9 years, 4 months ago)

hard to tell from OP but what kind of lids are you using?....:justdontknow:


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Peace Pot Micro-Dot God Loves You High or Not!!!
In order to grow old and wise, you must once have been young and dumb!

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: jboredone]
    #21068572 - 01/04/15 10:00 PM (9 years, 4 months ago)

current lids are the basic lid with a 1/4" hole punched in the center, a tyvek envelope cut to a disk placed between the lid and the metal ring and a s.h.i.p. to top it off.

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InvisibleInocuole
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21068718 - 01/04/15 10:34 PM (9 years, 4 months ago)

Is... the SHIP covering the GE hole?  How many holes are in the lid itself, exactly?


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Inocuole]
    #21068820 - 01/04/15 10:58 PM (9 years, 4 months ago)

I followed this out dated tek step by step. So the kids are the same as what you see here...
https://www.shroomology.org/topic/337-str0bes-no-fail-wbs-tek/

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InvisibleInocuole
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21069065 - 01/04/15 11:54 PM (9 years, 4 months ago)

Whoa... whoa whoa whoa.... I don't go to other mushroom sites.  You almost tricked me there, nuh uh, not today.  :nono:

Your fucking avatar though.  :lol:


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you

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Offlinespacechildo
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21069113 - 01/05/15 12:01 AM (9 years, 4 months ago)

that tek doesnt use ship's...
if you covered your  only hole with silicone you fuked up :sad:

I have a couple lid "teks" in my signature, you should check them out and stop using tyvek!

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: spacechildo]
    #21069254 - 01/05/15 12:49 AM (9 years, 4 months ago)

Disregard that, Wrong url. And the ship is on the tyvek not the actual lid where they were knocked up. No silicone between the whole in the jar and the tyvek, just ontop of the tyvek. jars seem to be doing fine maybe another day or two before they reach full colonization. Just looking for what lids that are needed for gtg. Even if my unconventional lids are incorrectly used the wbs still colonized with no contam. The teks I've read on here show the gtg pint jars but no lid prep. Only got a couple days before the jars are ready so looking for a full proof plan(suggestions)

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Invisiblejboredone
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Registered: 01/19/12
Posts: 4,798
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21069263 - 01/05/15 12:53 AM (9 years, 4 months ago)

did you shake those jars after 30 percent or so?....:justdontknow:


--------------------
Peace Pot Micro-Dot God Loves You High or Not!!!
In order to grow old and wise, you must once have been young and dumb!

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21069270 - 01/05/15 12:57 AM (9 years, 4 months ago)

Yea I shook them around there... Why? Looking at the jars today there's maybe 10/15% left to go

Tyvek was easy to get locally, any advice where to find filter disks locally? Why's the SFD lid necessary for gtg?

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Offlineganjfather
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: Thoky.ykoht]
    #21069272 - 01/05/15 12:58 AM (9 years, 4 months ago)

You just need a lid with a hole for GE. I make mine a little smaller than a dime. You need a filter over the hole, too. I use EZ felt but tyvek CAN work if it's all you have; you usually want to double layer it though.


If your lid has a hole for gas exchange you are good to go:thumbup:


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Invisiblewowimflabbergasted
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Registered: 07/16/12
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: ganjfather]
    #21069304 - 01/05/15 01:11 AM (9 years, 4 months ago)



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Offlineganjfather
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Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: wowimflabbergasted]
    #21069330 - 01/05/15 01:18 AM (9 years, 4 months ago)

:thumbup:  :thumbup:


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: 4 qts WBS 80%/100% colonized, WHAT NEXT?!? [Re: ganjfather]
    #21071334 - 01/05/15 01:34 PM (9 years, 4 months ago)

say you spawn 3 to coir or horse poo and stick the last one in the fridge, will it last till first flush? N00b to the agar. will two 20qt tubs be ideal when using all 4 or should it be a single tub?:confused:

can't stop laughing at ganjfather's avi

Edited by Thoky.ykoht (01/05/15 01:34 PM)

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