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OfflineOppiumNitrates
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Registered: 12/29/14
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Possible contam need help to identify
    #21060652 - 01/03/15 12:19 PM (9 years, 4 months ago)

First time cultivator here looking for opinions of more experienced cultivators. These jars have been incubating in a dark box at 80o for 18 day and were about 100% colonized 3-4 days ago.  I noticed that one of them has some darker very wet looking area on the bottom here is a pic



These two pictures are the side view of that same jar


All my other jars have a bottom that looks like this


I'm thinking that maybe the substrate was packed too tight into that jar and it just pressed against the bottom but would like some more experienced cultivators opinions.

Should I be concerned with that dark area on the bottom?

Thanks in advance.

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OfflinetaGyo
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Re: Possible contam need help to identify [Re: OppiumNitrates]
    #21060881 - 01/03/15 01:04 PM (9 years, 4 months ago)

That's bacteria.

Toss it.

If all your jars look like that I suspect you didn't Pressure Cook correctly.

Did you wait till 15 PSI to start your timer for 1 hour?


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Edited by taGyo (01/03/15 01:05 PM)

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OfflineMdahmer
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Re: Possible contam need help to identify [Re: taGyo]
    #21060915 - 01/03/15 01:12 PM (9 years, 4 months ago)

it does look like bacteria, what kind of incubator are you using? you really dont need to incubate them, cube mycelium does great in the 70's temp range, above 80 it starts to slow growth and favor bacterial growth. the inside of the cakes is hotter than the air surrounding them too so they could be stressing in there.

and you dont need to keep them in the dark for colonization. just set them on a shelf and wait.

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OfflinetaGyo
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Re: Possible contam need help to identify [Re: Mdahmer]
    #21060919 - 01/03/15 01:14 PM (9 years, 4 months ago)

Quote:

Mdahmer said:
above 80 it starts to slow growth and favor bacterial growth.




83f to be exact,
:thumbup:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum

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OfflineOppiumNitrates
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Re: Possible contam need help to identify [Re: Mdahmer]
    #21061097 - 01/03/15 01:40 PM (9 years, 4 months ago)

Quote:

taGyo said:
That's bacteria.

Toss it.

If all your jars look like that I suspect you didn't Pressure Cook correctly.

Did you wait till 15 PSI to start your timer for 1 hour?




As I said the bottom of all my other jars look like that fourth picture.  Do you think that forth picture also has bacteria?

I did not use a pressure cooker I followed the RR video where the jars are steam sterilized for 90 minutes.

Edited by OppiumNitrates (01/03/15 01:49 PM)

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Offlinemoricz
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Re: Possible contam need help to identify [Re: OppiumNitrates]
    #21061205 - 01/03/15 01:57 PM (9 years, 4 months ago)

Is that a PF cake? If it is, just birth it when all the other sides are colonised, maybe wait 2-4 days more. If bacteria is present you will smell some strange odours.

Don't care with it, just dunk and roll and put in an SGFC Fan and mist 2-4 times a day and wait, you'll probably get shrooms.
My GT ate my bacteriated grains, spawned for a lil test and fruited.

I prefer lower temps I never move over 24C° in the entire growing process.

My friend just birthed a smelly cake and the smell went away about 3 days later. The cake fluffened up and now pinning.

If you have other projects, birth them separate, you have nothing to loose.

GL!

Peace

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OfflineOppiumNitrates
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Re: Possible contam need help to identify [Re: moricz]
    #21061396 - 01/03/15 02:33 PM (9 years, 4 months ago)

I only have one SGFC and I do have other cakes, so would it be a problem if I put a cake with bacteria in with healthy cakes? Seems like a bad idea to me.

Also trying to get confirmation if the bottom of this jar is healthy or has bacteria also.  Most of the bottoms of my jars look like this.



--------------------
Guides me safely in Worlds I've never been to. Heal me Heal me, My dear Brena.

-=< A Perfect Circle - Brena >=-

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OfflinetaGyo
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Re: Possible contam need help to identify [Re: OppiumNitrates]
    #21061398 - 01/03/15 02:34 PM (9 years, 4 months ago)

Someone will chip in,

:thumbup:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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OfflineOppiumNitrates
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Re: Possible contam need help to identify [Re: moricz]
    #21061898 - 01/03/15 04:06 PM (9 years, 4 months ago)

Quote:

moricz said:
Is that a PF cake? If it is, just birth it when all the other sides are colonised, maybe wait 2-4 days more. If bacteria is present you will smell some strange odours.

Don't care with it, just dunk and roll and put in an SGFC Fan and mist 2-4 times a day and wait, you'll probably get shrooms.
My GT ate my bacteriated grains, spawned for a lil test and fruited.

I prefer lower temps I never move over 24C° in the entire growing process.

My friend just birthed a smelly cake and the smell went away about 3 days later. The cake fluffened up and now pinning.

If you have other projects, birth them separate, you have nothing to loose.

GL!

Peace




It is a PF cake.

Not sure what you mean by "My GT eat bacteriated grains"

Whan you say "birth my other projects separate" do you mean in a different FC?


--------------------
Guides me safely in Worlds I've never been to. Heal me Heal me, My dear Brena.

-=< A Perfect Circle - Brena >=-

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OfflinetaGyo
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Re: Possible contam need help to identify [Re: taGyo]
    #21062009 - 01/03/15 04:22 PM (9 years, 4 months ago)

Quote:

taGyo said:
Someone will chip in,

:thumbup:




--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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Offlinemoricz
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Re: Possible contam need help to identify [Re: OppiumNitrates]
    #21064343 - 01/04/15 01:30 AM (9 years, 4 months ago)

Yes different FC or make a quick one from PET bottles :laugh:

I'm not convinced they are bacteria it might be too wet and the bottom particles sticked to the glass and the myc cannot run into.

Open one and sniff it, if the smell is healthy you can drop into your FC

I strongly recommend lower temps like 22C° Moulds are slower and bacterias eaten.

MY GT is a golden teacher variety, they are strong, they ate bacteriated grain kernels, and in CVG bulk they fruited. Also oyster can eat bacterias finely if they are not in big numbers and the substrate is not too wet.

Good Luck!

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Re: Possible contam need help to identify [Re: moricz]
    #21064900 - 01/04/15 08:46 AM (9 years, 4 months ago)

Those other cakes look alright I think. Something about pictures always makes mycelium look weird to me but I think if I had a cake like that I wouldn't be worried.

You'll get used to the smell of cube mycelium real quick and most bacteria you'll be able to tell by smell too if y have it.

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InvisibleGuardian187
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Re: Possible contam need help to identify [Re: Mdahmer]
    #21064911 - 01/04/15 08:48 AM (9 years, 4 months ago)

It looks like the sub didn't fully colonize on the bottom (maybe too much water?). I've had jars where the mycelium looked a little "thin" and you could see the sub through it.

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OfflineOppiumNitrates
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Re: Possible contam need help to identify [Re: OppiumNitrates]
    #21065030 - 01/04/15 09:30 AM (9 years, 4 months ago)

So I opened that jar from the first picture and it smelled AWESOME!  I always loved the earthy smell of mushrooms, even before starting this hobby.  Here is a pick of the open jar.



I'm guessing that thin white stuff is myc that was able to creep through the dry vermiculite layer, maybe someone can confirm?

Thanks for the help.


--------------------
Guides me safely in Worlds I've never been to. Heal me Heal me, My dear Brena.

-=< A Perfect Circle - Brena >=-

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Offlinemoricz
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Re: Possible contam need help to identify [Re: OppiumNitrates]
    #21065078 - 01/04/15 09:56 AM (9 years, 4 months ago)

Look OK.

The smell was like the old cellar dirty or fresh mushroomy?

But still think those are fine, Just dunk&roll and fruit it. :thumbup:

Nothing to loose :smile:

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OfflineOppiumNitrates
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Re: Possible contam need help to identify [Re: moricz]
    #21065104 - 01/04/15 10:07 AM (9 years, 4 months ago)

It smelled like fresh mushrooms.


--------------------
Guides me safely in Worlds I've never been to. Heal me Heal me, My dear Brena.

-=< A Perfect Circle - Brena >=-

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InvisibleGuardian187
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Re: Possible contam need help to identify [Re: OppiumNitrates]
    #21065129 - 01/04/15 10:16 AM (9 years, 4 months ago)

:hahyeahwoo:

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OfflinetaGyo
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Re: Possible contam need help to identify [Re: Guardian187]
    #21065131 - 01/04/15 10:16 AM (9 years, 4 months ago)

:rockon:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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