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Defqon1
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Registered: 09/18/12
Posts: 144
Last seen: 6 months, 12 days
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Tupperware tek questions (pics)
#21059688 - 01/03/15 07:52 AM (10 years, 16 days ago) |
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I'm trying out these plastic glad tupperware containers for a pf run. I think I messed up the moisture content when i steamed them for 90 min, i think i didn't wrap the foil tight enough- at least i know i could have made it tighter. I just put tiny holes for GE didn't use tyvek, pollyfill or a self healing port. I do have pollyfill though i just would rather use the four tiny holes instead. Does anyone have experience using these plastic containers with four holes for GE. I decided to innoc with a syringe i made anyways to see how it goes although i don't have high hopes.
one of the containers was innoced with a questionable syringe that had a large mass of spores in it clumped together, you can see it in the pic its Albino A+.. Anyways I have more tupperware ready to fix whatever may be wrong with this particular try.
I opened up two just to see how the moisture on the inside was and the dry verm layer seemed to be okay, but i noticed what more visible condensation on the sides and top so i'm a bit worried.
I really want to make this tupperware tek work so any advice or links would be greatly appreciatied.
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Defqon1
Ubermenschen



Registered: 09/18/12
Posts: 144
Last seen: 6 months, 12 days
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Re: Tupperware tek questions (pics) [Re: Defqon1]
#21064632 - 01/04/15 06:21 AM (10 years, 15 days ago) |
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BumP* Funny how there's always lots of feedback when people are asking really dumb questions but i'm surprised there's been no response yet. Whatever, time will tell...
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LeopardMan
Constantly changing



Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: Tupperware tek questions (pics) [Re: Defqon1]
#21064664 - 01/04/15 06:53 AM (10 years, 15 days ago) |
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Never open your jars. Bad idea. 
I don't see anything that worries me in those pics and I don't understand your problem. Those spores were probably already germinated inside the syringe, that's why they were clumped together. It's not a bad thing per se (you should have faster colonization), but it's a waste of good spores. You used like 1000x the amount needed to inoculate a jar.
Let those jars sit and stop messing around with them.
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You have to die a few times before you can really live.
-Charles Bukowski-
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Jean-guy Masta
Railyard Ghost



Registered: 09/23/14
Posts: 1,827
Loc: MT-Hell
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Re: Tupperware tek questions (pics) [Re: Defqon1]
#21064673 - 01/04/15 06:59 AM (10 years, 15 days ago) |
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never did the tuppaware tek but for agar (eazy no pour tek) . i know water can get pretty eazy in those . you need a ball of paper towel between the lid and the aliminum cover also I suggest to fully cover the tuppaware with aluminium foil not just the lid .
Opening jars before its 100% always is a no no . how much time since innoc ?
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Edited by Jean-guy Masta (01/04/15 07:00 AM)
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Defqon1
Ubermenschen



Registered: 09/18/12
Posts: 144
Last seen: 6 months, 12 days
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Re: Tupperware tek questions (pics) [Re: LeopardMan]
#21064683 - 01/04/15 07:09 AM (10 years, 15 days ago) |
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LOL it came out all at once it was trippy to watch. I'm not worried about wasting syringes bc I've got lots atm and a few prints. I'm glad that you aren't worried. I threw out the ones I opened, obviously wasn't going to take any chances.
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Defqon1
Ubermenschen



Registered: 09/18/12
Posts: 144
Last seen: 6 months, 12 days
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Paper towel is a good idea, I'm guessing that would absorb any moisture that got threw the foil? I did wrap the whole containers but didn't make it as tight as I could have I'm going to do another trial run with another batch and modify make wrapping, also will try the paper towel. The tupperwares were innoced 1-2 days ago.
Edited by Defqon1 (01/04/15 07:15 AM)
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LeopardMan
Constantly changing



Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: Tupperware tek questions (pics) [Re: Defqon1]
#21064708 - 01/04/15 07:19 AM (10 years, 15 days ago) |
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Some condensation is normal
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You have to die a few times before you can really live.
-Charles Bukowski-
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Defqon1
Ubermenschen



Registered: 09/18/12
Posts: 144
Last seen: 6 months, 12 days
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Re: Tupperware tek questions (pics) [Re: LeopardMan]
#21324135 - 02/24/15 06:06 PM (9 years, 10 months ago) |
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just an update, all of these ended up colonizing fine. although when i tried to re-use them they all contamed. But this was likely an error on my part somewhere along the line.
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