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InvisibleJvF
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Cloning to BRF
    #21052406 - 01/01/15 01:48 PM (9 years, 4 months ago)

So about 4 days ago I inoculated a very small amount of BRF in a jar using polyfill as a filter. Its about a 1/4th inch tall. I used the tissue of a 2 day old shroom that I had sitting in the fridge in a paper bag.

1 of my jars has the tissue sitting on top of the brf and I managed to shake the tissue on a second jar under the brf.

The tissue was pretty beat up so it was almost black from bruising by time I was done.

Now its entirely black, a bit shriveled looking, and has absolutely no myc growth from it. its been 3 days.

Anyoje know why this happened?


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InvisiblebodhisattaMDiscordReddit
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Re: Cloning to BRF [Re: JvF]
    #21052409 - 01/01/15 01:49 PM (9 years, 4 months ago)

because that's a horrible way to clone, who said that was a good idea? link to your cloning TEK you used?

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InvisibleJvF
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Re: Cloning to BRF [Re: bodhisatta]
    #21052419 - 01/01/15 01:53 PM (9 years, 4 months ago)

No tek used, just made a bit of sense when I thought of it. It was more of a practice run in sterile technique on a shroom I had laying around. Just so id be a bit more familiar when my agar came in.


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OfflineTheMustardTiger
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Re: Cloning to BRF [Re: JvF]
    #21052499 - 01/01/15 02:12 PM (9 years, 4 months ago)

Quote:

jfischer218 said:
It was more of a practice run in sterile technique on a shroom I had laying around.




There's nothing sterile about using clone tissue from a dirty fridge mushroom to inoculate a jar of substrate.


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InvisibleJvF
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Re: Cloning to BRF [Re: TheMustardTiger]
    #21052543 - 01/01/15 02:19 PM (9 years, 4 months ago)

I was attempting the general act of cloning tissue.

I wasnt expecting it to work, im just more curious as to why it didnt/if anyone has been successful doing it.


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OfflineTheMustardTiger
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Re: Cloning to BRF [Re: JvF]
    #21052564 - 01/01/15 02:24 PM (9 years, 4 months ago)

Quote:

jfischer218 said:
I was attempting the general act of cloning tissue.

I wasnt expecting it to work, im just more curious as to why it didnt/if anyone has been successful doing it.




Well you said the tissue was black, this should have been a red flag. The tissue was dead.


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InvisiblebodhisattaMDiscordReddit
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Re: Cloning to BRF [Re: JvF]
    #21052581 - 01/01/15 02:27 PM (9 years, 4 months ago)

clone at the freshest available moment. if you have to put in the fridge use a paper bag but try to use within 24 hours. inner stem tissue taken with a sharp scalpel shouldn't be so damaged as to turn into slime. you ideally want to clone to a 2d medium like agar to ensure you have a clean culture before transferring it to a substrate like a cake.

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InvisibleGuardian187
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Re: Cloning to BRF [Re: bodhisatta]
    #21052625 - 01/01/15 02:46 PM (9 years, 4 months ago)

:facepalm:

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OfflineLogicaL ChaosM
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Re: Cloning to BRF [Re: JvF]
    #21052873 - 01/01/15 03:46 PM (9 years, 4 months ago)

I thought u clone mushrooms on petri dishes with agar then transfer clean wedges to a LC then inject BRF cakes?

I am missing something.


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Edited by LogicaL Chaos (01/01/15 03:47 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Cloning to BRF [Re: LogicaL Chaos]
    #21052882 - 01/01/15 03:48 PM (9 years, 4 months ago)

leave the LC step out why add another vector for contamination and more time lag to inoculation just use the agar directly and skip the syringe too

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OfflineLogicaL ChaosM
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Re: Cloning to BRF [Re: bodhisatta]
    #21052895 - 01/01/15 03:52 PM (9 years, 4 months ago)

No syringe?

But how to you get the agar into the BRF cakes without a needle?

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InvisibleGuardian187
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Re: Cloning to BRF [Re: LogicaL Chaos]
    #21054163 - 01/01/15 08:43 PM (9 years, 4 months ago)

From what I've seen most people go from agar to grain for inoculation. Could you collect mycelium from agar to a syringe -- similar to creating a spore syringe?

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OfflinecronicrFacebook
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Re: Cloning to BRF [Re: Guardian187]
    #21054182 - 01/01/15 08:50 PM (9 years, 4 months ago)

I think he did a brf paste


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Offlinemushpunx
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Re: Cloning to BRF [Re: LogicaL Chaos] * 1
    #21054196 - 01/01/15 08:52 PM (9 years, 4 months ago)

Quote:

LogicaL Chaos said:
No syringe?

But how to you get the agar into the BRF cakes without a needle?




Usually we drop the agar into a jar of grains but I have heard of people using a grain filter on a BRF jar and dropping a wedge on top of the brf. I dont know if it works but if its anything like grains it should nom nom nom the BRF.

:shrug: to me if you have the means to do agar work you have the means to use grain


Oh or you could blend up the agar into a liquid innoculant and use a needle I guess


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InvisiblebodhisattaMDiscordReddit
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Re: Cloning to BRF [Re: LogicaL Chaos]
    #21056178 - 01/02/15 10:47 AM (9 years, 4 months ago)

Quote:

LogicaL Chaos said:
No syringe?

But how to you get the agar into the BRF cakes without a needle?




not that hard. skip the dry verm layer and use a grain jar lid...

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InvisibleGuardian187
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Re: Cloning to BRF [Re: bodhisatta]
    #21056212 - 01/02/15 10:56 AM (9 years, 4 months ago)

Quote:

bodhisatta said:
Quote:

LogicaL Chaos said:
No syringe?

But how to you get the agar into the BRF cakes without a needle?




not that hard. skip the dry verm layer and use a grain jar lid...




Seems logical :smile:

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