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OfflinePinPornProducer
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Making an isolate question
    #21031435 - 12/27/14 11:09 AM (9 years, 4 months ago)

Okay, so let's say I want to try and create an isolate. I've done some research and think I have the process down. Clone tissue to agar, transfer best growth to other plates, transfer healthiest rhizo to new plates. Now what next? Cut a single stran of myc to transfer? Is that an isolate? Don't know when I'll get around to doing this. Might be a month from now or 2 years. Just want to have the knowledge under my belt so I can just go at it when I feel the urge. Thanks

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OfflinetaGyo
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Re: Making an isolate question [Re: PinPornProducer]
    #21031498 - 12/27/14 11:26 AM (9 years, 4 months ago)

Rhizo doesn't indicate fruiting potential.

IMO, clones are better then isolates. Clones have known fruiting potential. If you isolate you could be sectoring away from potency or fruiting capabilities. A good way to test this is to get the biggest shroom in the biggest cluster, rip it open, put a piece of tissue from the inside onto agar and let that grow. Then transfer a piece away, let it grow and fruit that to make sure you're on the right path.


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OfflinePinPornProducer
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Re: Making an isolate question [Re: taGyo]
    #21031514 - 12/27/14 11:31 AM (9 years, 4 months ago)

So with clones, what traits are carried on? Will pinsets be similar or just speed, potency and size?

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OfflinetaGyo
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Re: Making an isolate question [Re: PinPornProducer]
    #21031523 - 12/27/14 11:34 AM (9 years, 4 months ago)

Pinset, speed, potency and size will still vary slightly but generally a clone will produce more of the same. If you clone a big fruit, you will probably get larger then average fruits.

Azur, a member who grew a 200 gram penis envy shroom, cloned it and sent it to someone else who grew a 150-190 (Can't remember) gram shroom as well.


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OfflinePinPornProducer
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Re: Making an isolate question [Re: taGyo]
    #21031531 - 12/27/14 11:38 AM (9 years, 4 months ago)

That's really all I'm looking for is larger consistent fruits but try and keep a similar pinset.

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OfflinetaGyo
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Re: Making an isolate question [Re: PinPornProducer]
    #21031546 - 12/27/14 11:44 AM (9 years, 4 months ago)

Sounds like you're looking for a clone. Pick your biggest, most clustered fruit and clone it. Fruit that and repeat until your pinset and fruit size are what you want it to be.

Then save that culture forever,
After sending some to me :cool:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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OfflinePinPornProducer
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Re: Making an isolate question [Re: taGyo]
    #21031567 - 12/27/14 11:49 AM (9 years, 4 months ago)

I have the stuff for slants so I should just go ahead and do it and stop fkn around lol. Off topic ? What size hole in grain lids are best for sfds? I've been using micropore tape but finally got some sfds.

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InvisibleMunchauzen
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Re: Making an isolate question [Re: taGyo]
    #21031577 - 12/27/14 11:51 AM (9 years, 4 months ago)

Quote:

IMO, clones are better then isolates. Clones have known fruiting potential. If you isolate you could be sectoring away from potency or fruiting capabilities.




the idea is you are trying to sector away bad potency genes and bad fruiting capabilities, isolating the best genes from all the present strains. when you do find these good strains, they will indeed perform more predictably than a clone grow. its no doubt a lot more work. many more transfers and many more grows to test the strains. but in the end, good isolate>clone.

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OfflinePinPornProducer
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Re: Making an isolate question [Re: Munchauzen]
    #21031647 - 12/27/14 12:06 PM (9 years, 4 months ago)

3/8" holes work? I'm loading up some jars for pcing in about 20 min.

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OfflinetaGyo
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Re: Making an isolate question [Re: Munchauzen]
    #21031799 - 12/27/14 12:56 PM (9 years, 4 months ago)

Quote:

Munchauzen said:
Quote:

IMO, clones are better then isolates. Clones have known fruiting potential. If you isolate you could be sectoring away from potency or fruiting capabilities.



ts no doubt a lot more work. many more transfers and many more grows to test the strains. but in the end, good isolate>clone.




That's why I was suggesting he fruit as he transfer to make sure he's on the right path :thumbup:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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InvisibleTheChief
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Re: Making an isolate question [Re: PinPornProducer]
    #21031874 - 12/27/14 01:23 PM (9 years, 4 months ago)

Just toss a pin you like from a cluster on grain in the SAB.  :thumbup:

Don't worry about contams, if you do it in a SAB any contams on the pin will be outrun by the growth off of it; do it just like g2g, shake the jar at 20% colonization after dropping the pin.

If you want to work isolates you have to test each of them first before going large scale.  Like someone earlier said, there is a good chance you can isolate away from fruiting genetics trying to find a mono culture.

Edited by TheChief (12/27/14 01:34 PM)

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Invisiblemrbart4444
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Re: Making an isolate question [Re: taGyo]
    #21031878 - 12/27/14 01:24 PM (9 years, 4 months ago)

:whathesaid:
you'll hear a differnt answer from everybody but IMHO

clones > isolation


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OfflinePinPornProducer
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Re: Making an isolate question [Re: taGyo]
    #21031906 - 12/27/14 01:33 PM (9 years, 4 months ago)

Think I'll just try a clone first and see if I get the results I want. Never had any potency issues or anything. These things recover very fast. The only thing I would like to change is the fruit size on first flush, fruits from later flushes are perfect


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OfflinePinPornProducer
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Re: Making an isolate question [Re: PinPornProducer]
    #21031993 - 12/27/14 02:10 PM (9 years, 4 months ago)

A clone from a larger fruit would increase fruit size on first flush shouldn't it?

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InvisibleTheChief
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Re: Making an isolate question [Re: PinPornProducer]
    #21032001 - 12/27/14 02:12 PM (9 years, 4 months ago)

Look for a large and tight cluster, take the biggest pin from that cluster you like.  It is always better to clone pins because the genetics are younger and more aggressive, resulting in faster colonization down the line.  It is always best to clone the first pins from MS as well, the result of this is longevity because of young genetics and less cell division; most times people want to g2g their clones a bit and then fruit them out, the young genetics help a lot in that regard.

You should get some nice results by doing this.


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jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."

Edited by TheChief (12/27/14 02:43 PM)

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OfflinePinPornProducer
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Re: Making an isolate question [Re: TheChief]
    #21032025 - 12/27/14 02:21 PM (9 years, 4 months ago)

Drop it on agar or straight to grains?

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InvisibleTheChief
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Re: Making an isolate question [Re: PinPornProducer]
    #21032034 - 12/27/14 02:23 PM (9 years, 4 months ago)

Drop it right onto grain buddy, less time to wait that way.

A lot of people are so paranoid about contams it is unreal, I was too at one point, there are loop holes around contams if you know how they work though; this is one of them.  Pins grow so fast that as long as you transfer them onto clean grains in a still air environment they will outrun anything that landed on them while fruiting.


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."

Edited by TheChief (12/27/14 02:42 PM)

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OfflinePinPornProducer
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Re: Making an isolate question [Re: TheChief]
    #21032089 - 12/27/14 02:40 PM (9 years, 4 months ago)

Sounds good, I'll give this a shot tomorrow for sure

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OfflinetaGyo
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Re: Making an isolate question [Re: TheChief]
    #21032198 - 12/27/14 03:08 PM (9 years, 4 months ago)

Quote:

TheChief said:
If you want to work isolates you have to test each of them first before going large scale.  Like someone earlier said, there is a good chance you can isolate away from fruiting genetics trying to find a mono culture.



That was me.

:hahyeahwoo:


--------------------
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TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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OfflinePinPornProducer
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Re: Making an isolate question [Re: taGyo]
    #21032288 - 12/27/14 03:37 PM (9 years, 4 months ago)

If I had the space I would try to isolate but I'm stuck with room for just 3 monos.

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