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Amazon Shop for: Agar, HEPA Filter, Laminar Flow Hood, Pressure Cooker

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OfflineMadadaM
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would this work as a flowhood?
    #2102209 - 11/13/03 04:50 PM (13 years, 3 months ago)

a hepa furnace filter ( 20 x 20 ) glued to a fan of the same size.
would the air current be strong enough. if so this would only cost around 40 bucks.


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Invisiblemicro
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Re: would this work as a flowhood? [Re: MadadaM]
    #2102233 - 11/13/03 05:03 PM (13 years, 3 months ago)

Where's the air going? If it's just blowing out of the fan it won't really help. It might help a bit to blow the *sterile* air into what you're doing, but it's not a flowhood.

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OfflineMadadaM
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Re: would this work as a flowhood? [Re: micro]
    #2102432 - 11/13/03 06:16 PM (13 years, 3 months ago)

whats the difference?the fan would push air through the filter there by making sterile air flow where your working


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Invisiblemicro
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Re: would this work as a flowhood? [Re: MadadaM]
    #2102735 - 11/13/03 07:46 PM (13 years, 3 months ago)

Yeah -- an airflow with convections from unsterile air, too. It's in a room, so if you look at the airflow it will be somewhat straight in the center but will spiral off as you hit the edges. An actual laminar flow hood has an isolated air flow which makes it sterile. A glovebox is a step down, but is certainly better than a room -- you could hook a hepa filter up to the glovebox and have the air push into the glovebox from a fan through the filter making a somewhat positive-pressure environment so everything will leave the isolated air in the glovebox and not come in.

If you're talking about pushing the air from where you work into the HEPA it won't do anything.

(pardon the run-on sentance)

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Edited by micro (11/13/03 07:47 PM)


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OfflineOfaRevolution
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Re: would this work as a flowhood? [Re: micro]
    #2102820 - 11/13/03 08:12 PM (13 years, 3 months ago)

I have a small xbox fan going 27/7 for fruiting after casing, and i was wondering, should i have my fan blowing inside the whole set up(i have this in the bottom drawer of my bureau, and its lined wiht plastic then there is saran wrap on the top with holes in it) so im wondering if i should have the fan blowing in or out, if it blows in then where is the CO2 going because the drawrer is closed,obviovuly its not air tight but is it sufficient spacing enough for CO2 to get out, also if i have the fan facing out of the set up will it just suck out air and co2 and bring it agianst the side of the wall and bounce back in?? or will it escape.... there is a large hole in the saran wrap for the fan and the fan is facing up to blow stuff out sum1 plz tell me what the best way would be thanks alot


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Offlinewhitesox
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Re: would this work as a flowhood? [Re: OfaRevolution]
    #2102945 - 11/13/03 08:40 PM (13 years, 3 months ago)

This Micro is a idiot.Don't listen to him.His post is absolutley ridiculous.I think he is on here to try to unsaturate the market.


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OfflineMadadaM
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Re: would this work as a flowhood? [Re: whitesox]
    #2103148 - 11/13/03 09:31 PM (13 years, 3 months ago)

I still dont understand, if the fan is the same size (or mabye smaller)than the filter, placed behind the fiter, and this is blowing directly on your work space, why would it not work?


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InvisibleWyrdless
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In one word. No [Re: whitesox]
    #2103164 - 11/13/03 09:36 PM (13 years, 3 months ago)

The reason why it won't work is because a Furncae HEPA filter is not a 0.2 micron lab grade filter and won't be as effective. However, if I am wrong in this you still must create enough "static" air preasure to create a signifigant flow of air through the filter. A Hepa filter would require a great deal more than what could be provided by a box fan
The air current is needed to create a sterile work space

I suggest you make a simple glove box. Get a large box and put two holes in it. one for viewing and one for your hands. Cover the top with plastic sheeting and duct tape. Then fashion an opening with a 2 vertical splits. Put on large rubber gloves, use plenty of lysol and your set to go!

You will have a very clean enviroment which is contained from the outside and as long as you work quickly and using all of the common sense precautions, you'll have no contamination.


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Invisiblemicro
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Re: In one word. No [Re: Wyrdless]
    #2103226 - 11/13/03 09:54 PM (13 years, 3 months ago)

OFR -- this has nothing to do with casing. A flowhood is used for sterile work.

Whitesox -- please back up your argument and do not retort with no explanation on your behalf.... Or do we have some conflict of interest? I don't even know who you are....

Take any advanced physics class, or look up anything on flight instruction and see how air convections work.... Take the propeller and the wing for example. Look at the air flow -- once air hits an object it curls around it and creates a convection -- this is why the airplane "glides" when it is close to the ground.

--
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Offlinedebianlinux
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Re: In one word. No [Re: micro]
    #2103305 - 11/13/03 10:10 PM (13 years, 3 months ago)

micro has proven himself anything but an idiot. i will back him up 100% on this issue. for shame, I own a flowhood, a hepa blower, a glove box, and an oven (on which I have a standing rebuttal that it is ineffective). I have also taken several higher maths including several physics in my engineering studies and I can assure you that any simple fan with a HEPA filtered intake cannot offer laminar flow and therefore cannot offer ~100% sterile airflow. Even a laminar flowhood has a relatively short range of laminar flow. You might considering offering some pretty hard facts before insulting someone with the term "idiot".


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OfflineOfaRevolution
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Re: In one word. No [Re: debianlinux]
    #2103363 - 11/13/03 10:24 PM (13 years, 3 months ago)

Shot Down White Sox, he got u by the balls


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Invisibledeanofmean
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Re: would this work as a flowhood? [Re: MadadaM]
    #2103468 - 11/13/03 10:54 PM (13 years, 3 months ago)

a hepa furnace filter might be a good for a pre-filter .

here's an idea for ya, which i think explains what micro is talking about .

edit; take a look at the 2ed pic . what does Molester have stuffed in his shorts ?
Whitesox maybe ?


Edited by deanofmean (11/13/03 11:07 PM)


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OfflineRogerRabbitM
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Re: would this work as a flowhood? [Re: deanofmean]
    #2105560 - 11/14/03 11:22 AM (13 years, 3 months ago)

You talking about 'ground effect' micro? You a pilot?


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OfflineMadadaM
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Re: would this work as a flowhood? [Re: RogerRabbit]
    #2105588 - 11/14/03 11:34 AM (13 years, 3 months ago)

cant really flame sterilize a syringe to well in front of a fan can ya


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Offlineoptigram
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Re: would this work as a flowhood? [Re: MadadaM]
    #2105798 - 11/14/03 12:36 PM (13 years, 3 months ago)

swik uses such a filter as a pre-filter in his laminar flow hood, along with a standard furnace filter to trap the extra large particulate.
It has been my experience that doing things ghetto is just a waste of time. just starting out, you think you can do things on the cheap. all I can say is prepare yourself for failure. If youre new, you probably lack the skill to use super ghetto methods. good equipment compensates for human error. your technique doesnt have to be perfect if you have a flow hood and a decent pressure cooker. if you try to go ghetto, there is ZERO room for error.
don't laugh about the air currents, either. you think its funny, open a an agar plate outside the sterile stream. yeah, look at all that shit that just grew. I suggest you get something called a smoke pencil. it puffs out talc in a stream so you can watch air currents. check your thing with the smoke pencil, enlose the filter end and the fan end in open ended boxes at least six inches deep, and pray. because there is a good chance you are wasting your time.
spend the money, save the aggravation.


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OfflineAnnoA
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Re: would this work as a flowhood? [Re: optigram]
    #2105927 - 11/14/03 01:02 PM (13 years, 3 months ago)

>I suggest you get something called a smoke pencil.

AKA cigarette :wink: 


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Offlinedebianlinux
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Re: would this work as a flowhood? [Re: optigram]
    #2106007 - 11/14/03 01:24 PM (13 years, 3 months ago)

Quote:

optigram said:
good equipment compensates for human error




I'd rephrase that to say that good equipment negates many factors except human error. In the case of a flowhood human error is practically the only road to contamination. You actually have to make very deliberate and logical hand movements in your airstream and be careful to keep your cleanest equipment upstream in descending order of "dirtiness". In effect it takes skill to properly utilise a flowhood. In the hands of a novice the flowhhod is just as likely a vector of contamination as open air. Don't let "good equipment" give you a false sense of security. There is no substitute for practised aseptic technique.


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Invisiblemicro
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Re: would this work as a flowhood? [Re: debianlinux]
    #2106032 - 11/14/03 01:30 PM (13 years, 3 months ago)

Yeah -- just vfr, though :wink:

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Offlinedebianlinux
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Re: would this work as a flowhood? [Re: micro]
    #2106325 - 11/14/03 02:39 PM (13 years, 3 months ago)

Quote:

micro said:
Yeah -- just vfr, though :wink:



I just hope your referring to Volumetric Flow Rate and not Virulent Foot Rot :-)


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Invisiblemicro
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Re: would this work as a flowhood? [Re: debianlinux]
    #2106526 - 11/14/03 03:39 PM (13 years, 3 months ago)

Sorry -- I was answering Roger Rabbit -- vfr = visual flight rules -- it's a rating for a pilot's liscence.  I have a bad habbit of replying to the wrong person :wink:

I agree with you completely, though, debian -- my old (fat) boss contaminated everything he touched -- even when working in a laminar flow hood.  He'd touch the insides of caps and stuff -- the guy was a complete moron.  Needless to say I don't work for him, anymore -- I told his boss I'd quit if I did....  End result is I have a better job now.

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Amazon Shop for: Agar, HEPA Filter, Laminar Flow Hood, Pressure Cooker

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