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OfflineWorkmanV
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Psilocybe hispanica * 2
    #2102193 - 11/13/03 02:45 PM (20 years, 6 months ago)

Outdoor cultivated Psilocybe hispanica. This species is relatively easy to grow on sterilized horse manure placed outside during cool fall weather (around 60F). A relatively newly described species from Spain. In Spain it grows directly on sheep dung in fields that also support fruitings of Psilocybe semilanceata








Very small specimens growing from a displaced piece of colonized horse dung. Rodents or birds dug into the patch and scattered small pieces of dung that later fruited. It is interesting to note the reduced size of the mushrooms when growing from very small amounts of substrate.



This is a larger specimen from the main patch. Still a relatively small mushroom. The odor is very similar to Psilocybe semilanceata and the appearance reminds me of Psilocybe mexicana


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OfflinePaid
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Re: Psilocybe hispanica [Re: Workman]
    #2102207 - 11/13/03 02:50 PM (20 years, 6 months ago)

:thumbup: very interessting shrooms :grin:


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OfflinePolecat
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Re: Psilocybe hispanica [Re: Paid]
    #2102343 - 11/13/03 03:46 PM (20 years, 6 months ago)

very nice Workman...

tell me something about the trip that our fellow Iberic gives to us...

:smile:



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Re: Psilocybe hispanica [Re: Workman]
    #2102367 - 11/13/03 03:51 PM (20 years, 6 months ago)

Very interesting indeed !  :grin: 


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OfflineWorkmanV
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Re: Psilocybe hispanica [Re: Polecat]
    #2106240 - 11/14/03 12:20 PM (20 years, 6 months ago)

I have no idea of the potency since I don't eat mushrooms.


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OfflineAnnoA
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Re: Psilocybe hispanica [Re: Workman]
    #2107949 - 11/14/03 10:20 PM (20 years, 6 months ago)

Send them over here :wink: 

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OfflineCaptainFuture
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Re: Psilocybe hispanica [Re: Workman]
    #2213478 - 01/01/04 03:54 PM (20 years, 4 months ago)

Hi Workman!
What about sending some prints over to germany for further investigation???

Would be VERY kind...
Greez
Cap

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OfflineErik006
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Re: Psilocybe hispanica [Re: Workman]
    #2221154 - 01/05/04 05:14 PM (20 years, 4 months ago)

Looking good, what about the indoor cultivation of this species, has it been done, and can it be done?

Erik006


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OfflineWorkmanV
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Re: Psilocybe hispanica [Re: Erik006]
    #2228167 - 01/08/04 12:04 PM (20 years, 4 months ago)

I have observed primordia form on refrigerated agar so I do believe indoor cultivation is possible.


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Re: Psilocybe hispanica [Re: Workman]
    #2228293 - 01/08/04 12:45 PM (20 years, 4 months ago)

wanna pass around a spore print? ;-)


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OfflineErik006
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Re: Psilocybe hispanica [Re: Yarry]
    #2228645 - 01/08/04 02:42 PM (20 years, 4 months ago)

Sponsor the works and buy one, thats what im gonna do after when I get my ps. cyans and tamps ordered.

Erik006


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Workman]
    #24205371 - 03/30/17 01:13 PM (7 years, 1 month ago)

Could P. hispanica be conspecific with P. semilanceata?

The ITS sequences are just one base pair different, and it's just that one A turned into two A's near the beginning of the sequence, which is likely not even a real difference.

Perhaps they found P. semilanceata on horse dung and thought it was something new because of the unusual substrate.  But horse dung is really just chewed up grass, so it's not that much of a leap - and P. semilanceata is found in the area.

The LSU sequences of the two species are identical.

I am having trouble finding the original species description, which was published in "Docums Mycol. 29(no. 116): 42 (2000)".  Docums Mycol. is short for Les Documents Mycologiques.

Here's a photo of the holotype:  http://mushroomobserver.org/241329

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OfflineWorkmanV
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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #24205813 - 03/30/17 03:53 PM (7 years, 1 month ago)

I need to revisit this species. It is similar to P. semilanceata but it is much more responsive to cultivation, for whatever that is worth.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Workman]
    #24206168 - 03/30/17 06:12 PM (7 years, 1 month ago)

Perhaps a variety that has evolved to be more tolerant of different substrates.  Whether it should be called a separate species is hard to say, I wonder what they would look like cultivated side by side.....and if there are any microscopic differences.  I wonder if the difference in ITS sequences between P. hispanica and P. semilanceata would continue to be seen if more collections were sequenced.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #24211116 - 04/01/17 02:49 PM (7 years, 1 month ago)

i grew p hispanica in monotubs in a cold room this winter..temps were about 50-60F.  one of the easiest and most prolific species i have grown..


not sure about potency yet, havent had the time to try em


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Re: Psilocybe hispanica [Re: funkymonk22]
    #24211120 - 04/01/17 02:50 PM (7 years, 1 month ago)

How was the potency?  One person grew P. hispanica and said that nothing happened when the fruits were eaten.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #24211132 - 04/01/17 02:54 PM (7 years, 1 month ago)

you know, ive got mixed feedback from trusted friends..one got a buzz off a couple grams but then another ate 5g and got nothing. I will be sure to report back as soon as i try them myself...im actually watching one of your mexico mushroom excursions on youtube right now Allen:grin:

so cool, youve got me very interested in zapotecorum..just gotta locate spores


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Re: Psilocybe hispanica [Re: funkymonk22]
    #24219118 - 04/04/17 09:34 PM (7 years, 1 month ago)

so just to add to the confusion, i had 3 friend split 5g of hispanica and all had a mild but definite experience..i will be trying them soon and will offer up my bioassay to the pursuit of science:wink:


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Re: Psilocybe hispanica [Re: funkymonk22]
    #24225659 - 04/07/17 02:24 PM (7 years, 1 month ago)

Popcorn eating smiley, i hope that is helpful info

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Re: Psilocybe hispanica [Re: Lennybernadino] * 1
    #24239750 - 04/13/17 09:02 AM (7 years, 1 month ago)



couple hispanica shots from this morning..still fruiting away, they seem to love the PNW..i wonder if we can naturalize them here


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Re: Psilocybe hispanica [Re: Alan Rockefeller] * 2
    #24886937 - 01/03/18 08:38 AM (6 years, 4 months ago)

Quote:

Alan Rockefeller said:
Could P. hispanica be conspecific with P. semilanceata?

The ITS sequences are just one base pair different, and it's just that one A turned into two A's near the beginning of the sequence, which is likely not even a real difference.

Perhaps they found P. semilanceata on horse dung and thought it was something new because of the unusual substrate.  But horse dung is really just chewed up grass, so it's not that much of a leap - and P. semilanceata is found in the area.

The LSU sequences of the two species are identical.

I am having trouble finding the original species description, which was published in "Docums Mycol. 29(no. 116): 42 (2000)".  Docums Mycol. is short for Les Documents Mycologiques.

Here's a photo of the holotype:  http://mushroomobserver.org/241329




      I never saw Psilocybe semilanceata fruiting directly from cow patties or horse droppings.

      The Psilocybe hispanica strain I am working with does not look like Psilocybe semilanceata.

      It is easy to grow compared to Psilocybe semilanceata, a casing layer is not necessary. It is a cold weather species like Psilocybe semilanceata,
      fruiting between 40 and 60 F and the potency is definitely equal to Psilocybe semilanceata.

      But is the species I am working with really Psilocybe hispanica?

      And was the material used for DNA sequencing really Psilocybe hispanica?  Where did the material come from?  This is all very confusing.








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Edited by Sporulator (01/03/18 10:32 AM)

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OfflineMateo
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Re: Psilocybe hispanica [Re: Sporulator] * 1
    #24891943 - 01/05/18 01:53 PM (6 years, 4 months ago)

I saw pictures of a whole cluster P.Semilanceata fruiting directly from some animal poo.
It was a thread about it in a swedish forum back in 2012.
And it was Psilocybe semilanceata, im 100% sure of it having picked thousands of them.
We thought it was odd it fruiting directly on the poo like that.

In fact i have always thought P.Semilanceata has some connection to dung.
Where they grow in suburb areas over here they tend to grow grass lawns who has been newly laid out or grass lawn sown previous year.
Then they grow on these for a few years with declining numbers.
Just if they use up the nutrients or fertilizers in the soil.
And they always use animal poo of some sort as a fertilizer.
So P.Semilanceata is probably a dung lover, at least partially.


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Edited by Mateo (01/05/18 02:07 PM)

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Re: Psilocybe hispanica [Re: Mateo] * 1
    #24893131 - 01/05/18 11:04 PM (6 years, 4 months ago)

Quote:

Mateo said:
I saw pictures of a whole cluster P.Semilanceata fruiting directly from some animal poo.
It was a thread about it in a swedish forum back in 2012.
And it was Psilocybe semilanceata, im 100% sure of it having picked thousands of them.
We thought it was odd it fruiting directly on the poo like that.

In fact i have always thought P.Semilanceata has some connection to dung.
Where they grow in suburb areas over here they tend to grow grass lawns who has been newly laid out or grass lawn sown previous year.
Then they grow on these for a few years with declining numbers.
Just if they use up the nutrients or fertilizers in the soil.
And they always use animal poo of some sort as a fertilizer.
So P.Semilanceata is probably a dung lover, at least partially.




I agree with you that Psilocybe semilanceata is a nitrophilous species.

But I've never found them fruiting directly from dung here in Central Europe.

I always find them growing on former and now abandoned pastures, which have turned into ordinary grassland.

I suspect that another active species from Northern Spain could be involved in this confusion.

Psilocybe gallaeciae  described in 2003 for the first time by Guzman in this paper: 

http://www.samorini.it/doc1/alt_aut/ek/guzman03.pdf

http://mushroomobserver.org/observer/show_observation/59103

Edited by Sporulator (01/07/18 09:22 AM)

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OfflineMateo
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Re: Psilocybe hispanica [Re: Sporulator] * 1
    #24894023 - 01/06/18 11:59 AM (6 years, 4 months ago)

Intresting.

It wouldn´t suprice me if P.semilanceata has a few different sub varietys and that P.semilanceata and similar psilocybes are just same base mushroom that evolved to do well in their growing habitat.
How much must a mushroom change/evolve to be considered a new one?


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Re: Psilocybe hispanica [Re: Mateo] * 1
    #24895451 - 01/06/18 11:13 PM (6 years, 4 months ago)

Quote:

Mateo said:
How much must a mushroom change/evolve to be considered a new one?




      Good question!  But I'm a cultivator, not a taxonomist. You should ask Alan Rockefeller or Workman this question.

      I think an interesting experiment would be to grow mycelium of Psilocybe semilanceata and Psilocybe hispanica on the same agar plate.

      If they are the same species, no barrage formation should occur. Barrage formation means, that no nuclear exchange takes place, although hyphal fusions occur.


      https://link.springer.com/chapter/10.1007/978-3-642-69299-4_17


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OfflineMateo
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Re: Psilocybe hispanica [Re: Sporulator] * 1
    #24895839 - 01/07/18 07:35 AM (6 years, 4 months ago)

So if for example a mycel of say Psilocybe Cubensis variation Maztapec were grown on the same agarplate as Psilocybe Cubensis variation Golden teacher, the two would not form a barrier when they meet and mix smoothly as both are Psilocybe Cubensis?
They have probably grown separatley for many years and had time to adapt/evolve a bit of ther own.
But this should not matter then and no barrier form, that is intresting as it can be used as a tool to differentiate 2 mushrooms, just as you say.

Too bad my myco refridgerator has broken down, maybe i try this when i get a new one.
I think i have some spores of both P.semilanceata and P.hispanica.


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Re: Psilocybe hispanica [Re: Mateo] * 1
    #24895921 - 01/07/18 08:34 AM (6 years, 4 months ago)

Quote:

Mateo said:
So if for example a mycel of say Psilocybe Cubensis variation Maztapec were grown on the same agarplate as Psilocybe Cubensis variation Golden teacher, the two would not form a barrier when they meet and mix smoothly as both are Psilocybe Cubensis?




      Yes.


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Re: Psilocybe hispanica [Re: Sporulator] * 1
    #24898113 - 01/08/18 08:29 AM (6 years, 4 months ago)

Quote:

Sporulator said:
Quote:

Mateo said:
So if for example a mycel of say Psilocybe Cubensis variation Maztapec were grown on the same agarplate as Psilocybe Cubensis variation Golden teacher, the two would not form a barrier when they meet and mix smoothly as both are Psilocybe Cubensis?




      Yes.



:whathesaid:
Spores have absolutely no idea a human wrote a name on their syringe.
If they're the same species that's all they care about. And mating type


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OfflineMateo
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Re: Psilocybe hispanica [Re: bodhisatta] * 1
    #24905424 - 01/11/18 12:38 PM (6 years, 4 months ago)

But mushrooms evolve, change, adapt with time and environment.
So at some point they must have changed so much a new mushroom is developed.
Or it has at least changed enough to make a barrier on the agarplate.
Is´t that how every mushroom has allways been created?


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Mateo] * 1
    #24911907 - 01/13/18 08:09 PM (6 years, 4 months ago)

Quote:

Mateo said:
How much must a mushroom change/evolve to be considered a new one?





There is a central authority on nomenclature, but no central authority on taxonomy.  Therefore it is up to each individual person to answer that question for themselves, as there is no set number of changes that triggers it being new species.

I like to follow Dr. Bas's species concept, which is that there should be three unrelated differences.  They could be macromorphological, microscopic, chemical or molecular (DNA).

The biological species concept is a good way to answer this question in animals, but does not really work in plants or fungi since they hybridize often.

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Re: Psilocybe hispanica [Re: Alan Rockefeller] * 1
    #24914245 - 01/14/18 05:59 PM (6 years, 4 months ago)

Just an unrelated anecdote for anyone interested
https://en.m.wikipedia.org/wiki/Brassica_oleracea

broccoli, cauliflower, kale, Romanesco, Rapini, Brussels sprouts, collard greens, savoy, kohlrabi, and gai lan.
Are all the exact same species.
And all so very different. There's some you probably love and some you hate on that list.

Canines are like this too. There's obvious and vast difference between breeds


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Re: Psilocybe hispanica [Re: bodhisatta] * 1
    #25278290 - 06/19/18 07:22 AM (5 years, 10 months ago)

Quote:

bodhisatta said:
Just an unrelated anecdote for anyone interested
https://en.m.wikipedia.org/wiki/Brassica_oleracea

broccoli, cauliflower, kale, Romanesco, Rapini, Brussels sprouts, collard greens, savoy, kohlrabi, and gai lan.
Are all the exact same species.
And all so very different. There's some you probably love and some you hate on that list.

Canines are like this too. There's obvious and vast difference between breeds




Sorry to revisit an old thread, but Kale is the spawn of the devil!


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Re: Psilocybe hispanica [Re: MushroomOfLife] * 1
    #25284095 - 06/21/18 07:54 PM (5 years, 10 months ago)

Quote:

MushroomOfLife said:
Sorry to revisit an old thread, but Kale is the spawn of the devil!





Why?

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Re: Psilocybe hispanica [Re: Workman] * 10
    #26498787 - 02/22/20 05:20 PM (4 years, 2 months ago)

Quote:

Workman said:
Outdoor cultivated Psilocybe hispanica.  This species is relatively easy to grow on sterilized horse manure placed outside during cool fall weather (around 60F).  A relatively newly described species from Spain.  In Spain it grows directly on sheep dung in fields that also support fruitings of Psilocybe semilanceata








Very small specimens growing from a displaced piece of colonized horse dung.  Rodents or birds dug into the patch and scattered small pieces of dung that later fruited.  It is interesting to note the reduced size of the mushrooms when growing from very small amounts of substrate.



This is a larger specimen from the main patch.  Still a relatively small mushroom.  The odor is very similar to Psilocybe semilanceata and the appearance reminds me of Psilocybe mexicana   





I hope this puts a smile on your face workman wherever you are  =)

P. hispanica as a fun little cake




your teachings live on:mushroom2:



always your student,

sara




ps.

hi everyone:sun:


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Re: Psilocybe hispanica [Re: saralove] * 1
    #26500401 - 02/23/20 07:14 PM (4 years, 2 months ago)

Nice job! Did you have to do anything special compared to growing a normal cube?

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Re: Psilocybe hispanica [Re: Pliny_the_Elder] * 2
    #26500936 - 02/24/20 05:18 AM (4 years, 2 months ago)

holy shit. I have tried to germinate the print i have from sporeworks a  dozen times.
Amazing work!
I wish i could give you 5 more shrooms!

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Re: Psilocybe hispanica [Re: Workman] * 1
    #27106754 - 12/25/20 12:19 AM (3 years, 4 months ago)

Beautiful, OP! :kenthumbup:


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Re: Psilocybe hispanica [Re: saralove] * 1
    #27110417 - 12/27/20 09:50 AM (3 years, 4 months ago)

Quote:

saralove said:
Quote:

Workman said:
Outdoor cultivated Psilocybe hispanica.  This species is relatively easy to grow on sterilized horse manure placed outside during cool fall weather (around 60F).  A relatively newly described species from Spain.  In Spain it grows directly on sheep dung in fields that also support fruitings of Psilocybe semilanceata








Very small specimens growing from a displaced piece of colonized horse dung.  Rodents or birds dug into the patch and scattered small pieces of dung that later fruited.  It is interesting to note the reduced size of the mushrooms when growing from very small amounts of substrate.



This is a larger specimen from the main patch.  Still a relatively small mushroom.  The odor is very similar to Psilocybe semilanceata and the appearance reminds me of Psilocybe mexicana   





I hope this puts a smile on your face workman wherever you are  =)

P. hispanica as a fun little cake




your teachings live on:mushroom2:



always your student,

sara




ps.

hi everyone:sun:




What an awesome bump! Those are stunning! I'm going to watch this thread with interest.


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Re: Psilocybe hispanica [Re: coversall] * 3
    #27726779 - 04/09/22 06:28 AM (2 years, 1 month ago)



They are small, for me too easier to grow than semilanceata (as I failed all attempts with semi indoor) phenotype remind me a bit tampanensis but prefer colder temperature.. thanks to CaptainFuture for the print it was a something I wanted to observe since years and will work more on it! Fingers crossed for printing in few days!

Edited by V.L (04/09/22 09:23 AM)

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Re: Psilocybe hispanica [Re: V.L] * 1
    #27731885 - 04/12/22 07:28 PM (2 years, 1 month ago)

I believe that Psilocybe hispanica is a synonym of P. semilanceata or P. fimetaria.    The fruits in this thread look like P. fimetaria.

Someone should do some DNA work on these!

I'd be happy to but I don't have any material.

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Re: Psilocybe hispanica [Re: Alan Rockefeller] * 2
    #27732067 - 04/12/22 10:21 PM (2 years, 1 month ago)

Does a spore print is enough material? feel free to pm..

Psilocybe hispanica



hispanica printing

Those are the last pic I could upload, normal account limit reached :frown:


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Edited by V.L (04/13/22 06:53 AM)

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Re: Psilocybe hispanica [Re: V.L]
    #27732687 - 04/13/22 11:08 AM (2 years, 1 month ago)

Yes a spore print is good.    I sent you a PM and gave you unlimited image uploads.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27732789 - 04/13/22 12:20 PM (2 years, 1 month ago)

Cool! Thanks for pictures space, deleted few old pics today to upload but that’s really better! Will send a print to your way, if it can help to have more knowledge on them..


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Re: Psilocybe hispanica [Re: V.L]
    #27752867 - 04/27/22 02:17 AM (2 years, 22 days ago)

Took 2 weeks for a little second flush:



Some spores under my very old microscope and phone photo:



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Re: Psilocybe hispanica [Re: V.L]
    #27753944 - 04/27/22 07:38 PM (2 years, 22 days ago)

Are those dry spores?

How are you preparing the slide?

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27755057 - 04/28/22 12:50 PM (2 years, 21 days ago)

It was dry spores on slide then just one drop of water to separate them a bit and leave it until slide was dry again to not put water on the microscope.. I’m not practicing microscopy very often & was my first time with this specie. Did you received the print yet?


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Edited by V.L (04/28/22 01:41 PM)

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Re: Psilocybe hispanica [Re: V.L] * 1
    #27755634 - 04/28/22 08:03 PM (2 years, 21 days ago)

It hasn't arrived yet unless it was in the little stack of mail that I brought to the lab the other day. 

Spores are generally studied when hydrated - yours look a bit collapsed, which is what they are like when they are dry.

I suggest rehydrating them with a half drop of 5% KOH, then putting down a coverslip, lifting it up slightly and putting it back down until air bubbles are gone.  Optionally paint a ring of nail polish around the edge of the coverslip.    Observe using the 100x oil immersion objective, with a drop of immersion oil on top of the coverslip.  If you don't have immersion oil you can use vegetable oil or even isopropanol in a pinch.

If you don't have KOH you can use 70% isopropanol, then let it half dry by blowing on it for 20 seconds or so, then add a half drop of water and put down the coverslip.

If you have a condenser under the stage, open and close it until the image looks best.  It generally should be more closed with the lower power objectives and more open at the higher magnifications.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27756033 - 04/29/22 02:32 AM (2 years, 20 days ago)

Thanks for all the info & advice about microscopic practice it’s an other world!! even I’m usually more into macroscopic observations and printing. I did a 2nd cake from more isolated culture and it’s pinning like crazy! Will share some pics if they mature well.. have a nice day


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Re: Psilocybe hispanica [Re: V.L] * 8
    #27762299 - 05/04/22 02:01 AM (2 years, 15 days ago)

2nd cake 1st flush


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Re: Psilocybe hispanica [Re: Alan Rockefeller] * 1
    #27762497 - 05/04/22 08:43 AM (2 years, 15 days ago)

I agree Alan, the ones in this thread do look similar to P.fimetaria. I'll attach some examples of hispanica-looking-fimetaria below.

If P.hispanica is actually P.fimetaria, it would mean we would be able to add sheep manure to the list of substrate from which P.fimetaria grows. Also, I have a suspicion that P.fimetaria might grow from Red Deer manure - at least in Scotland/UK.

I saw in another comment, from a few years ago, you said you had an ITS and LSU sequence of P.hispanica. Have you tried comparing the ITS to one of P.fimetaria?














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Re: Psilocybe hispanica [Re: DH42]
    #27762612 - 05/04/22 10:06 AM (2 years, 15 days ago)

Yes maybe they are conspecific with fimetaria (I don’t think with semilanceata) I hope Alan will receive the prints and could give the final answer with genome sequencing.. personally really love how they look! hispanica or even if it’s same with fimetaria.. do you or someone have a fimetaria prints? so I can work also on it to try to do a comparative grow. And does anyone know a bit more about hispanica/fimetaria usual potency?


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Re: Psilocybe hispanica [Re: V.L] * 5
    #27762754 - 05/04/22 12:15 PM (2 years, 15 days ago)

I think Psilocybe alutacea should be included in these comparisons.


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Re: Psilocybe hispanica [Re: V.L] * 1
    #27765544 - 05/06/22 07:07 AM (2 years, 13 days ago)

Quote:

V.L said:
Yes maybe they are conspecific with fimetaria (I don’t think with semilanceata) I hope Alan will receive the prints and could give the final answer with genome sequencing.. personally really love how they look! hispanica or even if it’s same with fimetaria.. do you or someone have a fimetaria prints? so I can work also on it to try to do a comparative grow. And does anyone know a bit more about hispanica/fimetaria usual potency?





Perhaps they are conspecific with P.fimetaria. You are right - they are definitely different to P.semilanceata. However, they are undoubtedly closely related.

I do have some P.fimetaria prints.

Potency for P.fimetaria (and perhaps, by extension, P.hispanica) is an unresolved topic. From what I have observed and been told, the potency varies considerably for P.fimetaria. Most often it is considerably less potent than, say, P.semilanceata. However, some people report effects on par with P.semilanceata gram-for-gram. The issue ties in with the (lack of) blue bruising found on most P.fimetaria specimens. I believe it might be something to do with differing substrate. I think cow manure produces weak specimens whereas horse manure can produce more moderate ones. However, the existence of P.liniformans confuses things considerably. It looks practically identical to P.fimetaria and the only differing macroscopic feature is the presence of a separable gelatinous gill edge. So, I believe some of the people who have reported strong trips off P.fimetaria *might* have been actually consuming P.liniformans. More work is needed!


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Re: Psilocybe hispanica [Re: inski] * 3
    #27765546 - 05/06/22 07:08 AM (2 years, 13 days ago)

Quote:

inski said:
I think Psilocybe alutacea should be included in these comparisons.






Another potential candidate for P.fimetaria!



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Re: Psilocybe hispanica [Re: DH42] * 1
    #27766754 - 05/06/22 11:47 PM (2 years, 12 days ago)

Thanks Insky & DH42 interesting! I only had few fruits on my alutacea attempt years ago and last tries it didn’t germinate (probably too old) Feel free to PM to exchange few prints ; ) to try to compare all of them.. have a beautiful weekend!


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Re: Psilocybe hispanica [Re: V.L] * 1
    #27776389 - 05/14/22 01:55 AM (2 years, 5 days ago)

Hey just popping by to post some photos of a few fruits from this years semilanceata grow.
I will send these away for sequencing soon and will post the result.


Edited by Baba Yaga (06/15/22 03:41 PM)

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Re: Psilocybe hispanica [Re: Baba Yaga]
    #27779682 - 05/16/22 12:40 AM (2 years, 3 days ago)

Quote:

Baba Yaga said:
Hey just popping by to post some photos of a few fruits from this years semilanceata grow.
I will send these away for sequencing soon and will post the result.




Very cool!  When you get data PM me if you want to do a quick zoom call - I can share my screen show you how I analyze sequence data using free tools and put it into Genbank.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27779822 - 05/16/22 05:01 AM (2 years, 3 days ago)

That would be great Alan.

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Re: Psilocybe hispanica [Re: Baba Yaga]
    #27780540 - 05/16/22 04:31 PM (2 years, 3 days ago)

very nice - on the assumption that they are growing from dung (which it looks like they are) they may well be P.fimetaria!


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Re: Psilocybe hispanica [Re: DH42]
    #27780714 - 05/16/22 07:25 PM (2 years, 3 days ago)

At this stage anything is possible. This is grain spawned to potting mix by the way,
no manure in there at least it didn't say anything about it on the bag.

Still hoping that these are semilanceata since none of the cultivated specimens
really look like what you can find in the wild.

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Re: Psilocybe hispanica [Re: Baba Yaga]
    #27783867 - 05/19/22 06:05 AM (2 years, 16 hours ago)

Joining this thread too, because of all the good info and the recent development.

Its going to be super exciting to follow hispanica/semilanceata/fimetaria complex.

Got a lot of small individual subs going outside, inside, etc

Im sure we'll have more answers by end of 2022

-

Quote:

funkymonk22 said:
i grew p hispanica in monotubs in a cold room this winter..temps were about 50-60F.  one of the easiest and most prolific species i have grown..


not sure about potency yet, havent had the time to try em





Also to me, the tub here is very alike to my sequenced p fimetaria


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Edited by smalltalk_canceled (05/24/22 03:14 AM)

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27786235 - 05/20/22 09:35 PM (1 year, 11 months ago)

Quote:

Alan Rockefeller said:
Quote:

Baba Yaga said:
Hey just popping by to post some photos of a few fruits from this years semilanceata grow.
I will send these away for sequencing soon and will post the result.




Very cool!  When you get data PM me if you want to do a quick zoom call - I can share my screen show you how I analyze sequence data using free tools and put it into Genbank.





So I'm going to tell VL that he should send you another sample, I told him how my tamp sample was misplaced as well.  I want you to do that for me man, I've been dying to know for over a year now~!  I know your in GA right now and have been busy doing conventions!

I'll private message you but I'm putting this part here so you have a responsibility :wink:


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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27786393 - 05/21/22 01:43 AM (1 year, 11 months ago)

Hey Alan. Did you ever find out what they were?

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Re: Psilocybe hispanica [Re: MycoCakes] * 4
    #27812106 - 06/09/22 11:27 AM (1 year, 11 months ago)










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Edited by smalltalk_canceled (06/09/22 04:55 PM)

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Re: Psilocybe hispanica [Re: smalltalk_canceled] * 5
    #27883715 - 07/31/22 04:52 AM (1 year, 9 months ago)

Ok, so I got the sequence results back and the mushrooms I grew are semilanceata from what I can see.

A few examples from the first Semilanceata Grow 2020:




and some fruits from second grow 2022 started with spores from above. Samples for sequencing were taken from this grow:

 


Because I banned myself for a few weeks I couldn’t get in touch with Alan but instead I watched his videos on youtube about how to work the
data and this is what I got out from alingning the sequence with BLAST and creating a phylogenetic tree with Geneious Prime.

Alan, I can send you the .ab1 file if you want to have a look at that.


Sequence:

ACCTGATTTGAGGNCAAATTGTCATTTGTATTGTCCAGTGAAGGACGGTTAGAAGCAGCGCAATCCCATTCATGCAAAGGTCCACGGCGTAGATAATTATCACACCAATAGACGGCTCTGCGCGGGGCACCGGCTAATACATTTAAGGGGAGCAGACCTCTTGACGAAGCCAGCAAAAGACCCCCACATCCAAGCCATTATCAGCAAAAGCTGGTAAGGTTGAGAATTTAATGACACTCAAACAGGCATGCTCCTCGGAATACCAAGGAGCGCAAGGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTGTATATAGTTTATAGGCACAAGGCCAATATAATACATTCTGTTACATTCTTTGGGGTATATGAAAACGTAGGCCTGGGTTAATTTGCAAGGAGAGCTTGTGAAAGCAATCCTCTTGACCGAGTTTCCTCGGAAAGTTAACTAATCCAGGTCTACAAAAGGTGCACAGGTGGAGAGATAAAGATGACACGGCGAGCACATGCCCCCGAGAGGACCAGCTACAACCGAGCCAAGTTCATTCAATAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAAACCAAGGAAA


Alignment with the top match in Blast showing only one difference between the two and a phylogenetic tree made with Snap-Gene:




This should be right, right? Since I am not a pro in this regard I would appreciate if you could confirm my findings Alan so I can re-post these
results in a few other places like the Hunting&ID Forum, the Misc Exotic Thread  and include it in the introduction of the Official Sem Thread as well.

Edited by Baba Yaga (08/02/22 04:38 PM)

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Re: Psilocybe hispanica [Re: Baba Yaga]
    #27886287 - 08/02/22 12:25 AM (1 year, 9 months ago)

Yes definitely semilanceata.  I replied to your PM.

It would be cool to put these photos on Mushroom Observer, upload the sequence data to Genbank and link the Genbank record to the Mushroom Observer record so there's a permanent record of this, it'd be good to be able to refer to it when looking at possible semilanceata photos.

A good way to share .ab1 files is to put it on a public Google drive link.

You can also contact me on gmail, Facebook messenger and IG, I use the same name on all 3.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27886306 - 08/02/22 01:09 AM (1 year, 9 months ago)

Thanks Alan, there is no reply in my inbox though. I have your gmail address and will get in touch soon.

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Re: Psilocybe hispanica [Re: Baba Yaga] * 1
    #27889702 - 08/04/22 12:07 PM (1 year, 9 months ago)



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Re: Psilocybe hispanica [Re: smalltalk_canceled]
    #27903560 - 08/14/22 07:07 PM (1 year, 8 months ago)



Does this mycelium look like agaricus blazeii or like hispanica


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Re: Psilocybe hispanica [Re: Baba Yaga]
    #27909455 - 08/19/22 11:11 AM (1 year, 8 months ago)

Nice One Baba Yaga :thumbup:


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Re: Psilocybe hispanica [Re: smalltalk_canceled]
    #27923995 - 08/29/22 12:09 PM (1 year, 8 months ago)

Quote:

smalltalk_canceled said:

Does this mycelium look like agaricus blazeii or like hispanica





It is hard to see what is going on here, but I would guess a mold.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27924010 - 08/29/22 12:23 PM (1 year, 8 months ago)

Alan did you received/sequenced the sample I sent you?


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Re: Psilocybe hispanica [Re: V.L]
    #27924013 - 08/29/22 12:24 PM (1 year, 8 months ago)

Quote:

V.L said:
Alan did you received/sequenced the sample I sent you?




What is the iNaturalist or Mushroom Observer observation number?

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Re: Psilocybe hispanica [Re: Alan Rockefeller] * 1
    #27924020 - 08/29/22 12:31 PM (1 year, 8 months ago)

It was from this grow:

Sent from France few months ago


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Re: Psilocybe hispanica [Re: V.L]
    #27951596 - 09/15/22 03:40 PM (1 year, 7 months ago)

I was on a hunt may/june in Pyrenees but no luck. Spoke to a locals and heard I can find them if I got luck. Gotta do a 2nd attempt. Where do you guys find yours ??

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Re: Psilocybe hispanica [Re: KevinEm]
    #27954082 - 09/17/22 07:14 AM (1 year, 7 months ago)

Got the prints from CaptainFuture


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Re: Psilocybe hispanica [Re: V.L] * 1
    #28011128 - 10/22/22 06:20 PM (1 year, 6 months ago)


Right now doing a little comparative experience inoculated both cakes same day one with semilanceata (from Netherlands) the other with hispanica, both also put in fruiting condition same day, hispanica already started pinning but not the semilanceata yet, still keep hope for it when temperatures will be fresher..


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Re: Psilocybe hispanica [Re: V.L] * 1
    #28495629 - 10/07/23 04:25 PM (7 months, 7 days ago)

Finally got back to this species after 19 years (WTF!). Still grows easy outdoors.



Quote:

saralove said:

I hope this puts a smile on your face workman wherever you are  =)

P. hispanica as a fun little cake



your teachings live on:mushroom2:

always your student,

sara

ps.

hi everyone:sun:




Yes, made me smile. Thank you saralove if you are still around.


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Edited by Workman (10/07/23 04:33 PM)

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Re: Psilocybe hispanica [Re: Workman]
    #28495640 - 10/07/23 04:42 PM (7 months, 7 days ago)

She pops in from time to time when the kids give her a break. :lol:

I think I have a print from that grow. Need to get back to them myself.


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Re: Psilocybe hispanica [Re: Workman]
    #28495700 - 10/07/23 05:46 PM (7 months, 7 days ago)

I have a couple of prints of Ps. Hispanica from FSE which I'm struggling to get any germination from.

Hope to join you all in growing this species soon.



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Edited by covertjoy (10/07/23 08:26 PM)

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Re: Psilocybe hispanica [Re: covertjoy]
    #28495845 - 10/07/23 08:06 PM (7 months, 7 days ago)

its all a mess


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Re: Psilocybe hispanica [Re: smalltalk_canceled] * 2
    #28495997 - 10/07/23 10:35 PM (7 months, 6 days ago)

A friend ITS sequenced Psilocybe hispanica that he got from someone (VR or something) and it was a 100% match for Psilocybe fimetaria.

I also saw a photo of P. hispanica that the person who discovered it took, and it looked just like P. fimetaria, so I think that's what these are.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #28496002 - 10/07/23 10:43 PM (7 months, 6 days ago)

Hmmm, it does look identical. I'll get my sample tested and report back. I also have some fimentaria and liniformans to grow out and compare.


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Re: Psilocybe hispanica [Re: Workman]
    #28496244 - 10/08/23 08:18 AM (7 months, 6 days ago)

without knowing it, just found some of them a couple of days ago....

https://www.shroomery.org/forums/showflat.php/Number/28494130/page/1

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Re: Psilocybe hispanica [Re: el gordo] * 1
    #28496427 - 10/08/23 11:28 AM (7 months, 6 days ago)

It is very likely that P.hispanica is really P.fimetaria. I am in contact with the finder of the holotype and will hopefully get a few sequences of 'P.hispanica; in a month or two. The pictures that Alan refers to above were taken by this same person.

However, I think it is not impossible that it may be a distinct taxon. I have the ITS and TEF1 sequences from the P.hispanica holotype; the TEF1 doesn't really match to anything and the ITS is too incomplete to get anything very useful from it. The holotype was collected in the 1990s, so a fresh 'P.hispanica' from the original collector should confirm whether or not the species is a synonym. P.fimetaria certainly grows throughout the Pyrenees, but perhaps there is another species hiding there? I will update later in the year once we (hopefully) know!


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Re: Psilocybe hispanica [Re: Workman]
    #28497382 - 10/09/23 08:45 AM (7 months, 5 days ago)

Quote:

Workman said:
Hmmm, it does look identical. I'll get my sample tested and report back. I also have some fimentaria and liniformans to grow out and compare.





I've put P. fimetaria and P. liniformans sequences in Genbank for comparison.

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Re: Psilocybe hispanica [Re: Workman] * 1
    #28499448 - 10/10/23 09:55 PM (7 months, 4 days ago)

From a print from a friend named HB from germany, Hispanica (?) growing outside in the bay area CA, will post updates

Edited by fireinyoface (10/10/23 10:40 PM)

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Re: Psilocybe hispanica [Re: fireinyoface] * 1
    #28499682 - 10/11/23 06:33 AM (7 months, 3 days ago)

Reviewing this thread and it's pictures, it seems very evident to me that the fimetaria and hispanica is either the same, or extremely closely related.

Hopefully this theory can now be used to actually check/find out

Fimetaria/Hispanica from today, surviving from 2022






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Edited by smalltalk_canceled (10/14/23 03:00 PM)

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Re: Psilocybe hispanica [Re: smalltalk_canceled] * 1
    #28499825 - 10/11/23 09:28 AM (7 months, 3 days ago)

:popcorn:

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Re: Psilocybe hispanica [Re: murderlabz] * 4
    #28504415 - 10/14/23 02:26 PM (7 months, 7 hours ago)

For posterity Im linking up a selection of the then cold fruiting hispanica fruiting "outside" as a flowerpot, fruiitng in a cold garage, and fruiting placed outside in fall, and fruiting from one year to another although very sparsely so

this, evidentily, and ofc a bit to my dismay, was psilocybe hispanica all along:








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Edited by smalltalk_canceled (10/14/23 02:31 PM)

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Re: Psilocybe hispanica [Re: smalltalk_canceled] * 3
    #28515555 - 10/23/23 05:26 PM (6 months, 22 days ago)

Hey guys!

I grew the suppossedly hispanica from the spores i got from the user Poison Drink, who got them from Captain future (and i think he got them from Workman?). I assumed the ID was done correctly by the original collector even though I have slight doubts as unlike the original description the stipe doesn’t bruise blue and the fruits also show some veil (the key in Noordeloos monography about Strophariaceae s.l says it lacks veil).




I also managed to find an article from 2006 in a spanish magazine where someone talks about this species being found apparently in Euskadi (a región near Pyrinees but not as high), but seeing the description, the spore pics and the macroscopical details (like the cap resembling more like P. semilanceata) i got a bit confused, cause it doesn’t look like the supossedly P. hispanica from most grows posted on Shroomery. But on the other hand it is pretty common that indoor or cultivated mushrooms end looking really different from wild ones, so…

The following pics and description are from the specimens that were sent by the author of the article to Guzman for confirmation and also the same ones from which the genbank sequence KC669289.1 came from (Fernandez-Sasia's voucher). The suppossedly hispanica imo shows more resemblance to P. semilanceata. Afaik P. semilanceata doesn't fruit from dung, but it is not rare that some fruits can be found fruiting through dung cause the mycelium being underneath the poo. Could that have lead to the false asumption that it was a different species (cause the collector thought it was not semilanceata cause of dung)?



If you search this hispanica sequence in genbank (KC669289.1), after running blast you see a 100% match with P. semilanceata sequences, so probably this article was written upon a misidentification made by the author of the article and later also (mistakenly?)ratified by Guzmán.

It also gets my attention the spore micrographs which show a highly truncated germ pore that i think i couldn’t see on the spores from my grow, but perhaps it is caused by the low quality of my microscope which causes some aberration?

All that said, i had samples from my grow sequenced and results in genbank apparently matched it with one of the P. fimetaria  sequence uploaded to genbank. So perhaps the "hispanica" i grew is just P. fimetaria and either there was a misidentification and the true hispanica keeps lost and waiting to be found again or it is just that both are synonyms? But which species could be synonyms? P. hispanica and P. fimetaria or P. hispanica and P. semilanceata?
:badtrip:

P. liniformans is easily distinguished by the gelatinous lamella edge, at least on fresh specimens, idk if dried samples when rehydrated show that elasticity (i have found P. liniformans in NW spain and in north portugal too and in same dung some fruits have elastic lamella edge and other bigger fruits from the same dung not having that separable edge when fresh). I wonder if that elasticity is lost when fruits mature or what, but it should be possible to check under microscope the gelatinous layer with cresyl blue i guess. I should check the samples i keep in my fungarium cause in one dung sample i found fruits that resembled to me more of fimetaria (showing convex cap with acute umbo and what seems like veil with spores) and others more like liniformans (i could send some samples to you Alan if you want to check them or sequence). The ones that were consistent with the liniformans description were sequenced and matched with the liniformans sequence that Alan found on Netherlands few years ago.


Guzmán said that P. hispanica is different from fimetaria cause hispanica lacks veil and has larger cheilocystidia, and different from semilanceata cause of the acute umbonate pileus... but seeing the grows from P. semilanceata that shows that unusual morphology on the cap, it seems like morphology can be a bit wobbly sometimes when it comes to name new species, as it can show some variation due to causes that can be overlooked (i.e. environmental conditions). It happened with Amanita porrinensis which was described as new species in 1980 and found in two subsequent years and never again... only once time more in italy apparently. After sequencing the samples, it was said to be a form of Amanita phalloides.


Edited by Inocybe (10/24/23 02:34 AM)

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Re: Psilocybe hispanica [Re: Inocybe] * 4
    #28516577 - 10/24/23 01:12 PM (6 months, 21 days ago)

Nice work. I've got some P. fimetaria from the Netherlands growing side by side with the hispanica outdoors and they do look different, for whatever that is worth. Hispanica on the left, fimetaria on the right. Hispanica fruited first so it seems to do better in warmer weather and is more robust. But the fimetaria was growing on a less than ideal substrate, so I need to start again to get better data. If they are both fimetaria, the hispanica seems to be the better variety for cultivation.



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Re: Psilocybe hispanica [Re: Workman]
    #28516763 - 10/24/23 03:48 PM (6 months, 21 days ago)

i never sent you a print workman, what out out what i did is still in interest, if anything?

I was able to print the 2023 fruit. i have one inky print of it

and i believe there could be 3 mre print from the original "fimetaria" now turned hispanica collection

at this point


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Edited by smalltalk_canceled (10/24/23 03:51 PM)

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Re: Psilocybe hispanica [Re: Inocybe]
    #28516873 - 10/24/23 05:05 PM (6 months, 21 days ago)

Inocybe, great write up, thank you for sharing that!

The genbank sequence KC669289.1 is, as it does appear, P.semilanceata. I have discussed this with Fernandez-Sasia. P.semilanceata fruiting underneath dung can produce some strange and confusing results, particularly in giving the illusion of it actually growing from dung in the same way that any obligately coprophilous fungus would.

Guzman's description of P.hispanica is faulty. I am in contact with the finder of the original holotype and hopefully something useful will be sequenced this year relating to it. P.hispanica's taxonomic position is certainly confusing, and we can't rush to synonymise it with P.fimetaria or P.liniformans prematurely.

Also, the species all display some level of polymorphism when cultivated artificially, which is interesting!


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Re: Psilocybe hispanica [Re: DH42] * 2
    #28519180 - 10/26/23 05:34 PM (6 months, 19 days ago)










Here's an update

Edited by fireinyoface (10/30/23 05:43 AM)

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Re: Psilocybe hispanica [Re: fireinyoface] * 1
    #28519756 - 10/27/23 03:45 AM (6 months, 18 days ago)

Lovely pics of...Psilocybe fimetaria


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Re: Psilocybe hispanica [Re: DH42]
    #28519921 - 10/27/23 09:15 AM (6 months, 18 days ago)

It was given to me as Hispanica but I will just refer to it as Fimetaria from now on! I might send it in for sequencing but I think its Fimetaria also.

Edited by fireinyoface (10/27/23 11:01 AM)

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Re: Psilocybe hispanica [Re: fireinyoface]
    #28520548 - 10/27/23 08:23 PM (6 months, 18 days ago)

My 2023 "hispanica" spore printed a decidely black or dark, over brown

So when Captain future was sending out spore prints named ps hispanica

to my experience faint brown or black, pitch in here

where did he get his classification from to call it hispanica, instead of fimetaria? at the point he did?

is the swedish artsdatabanken.se reporting ps hispanica or ps fimetaria? when it says there are 6 observations there? (gov)

what does the literature or the experts so gathered say about the following:

1: was ps hispanica a cold fruiter
2: was there ever a fimetaria _before_ hispanica, or was fimetaria identified at the same time or later than hispanica
3: what is the history of the spore colour of fimetaria and hispanica
  and is any concentration of these even wildly possible to be mistaken for semilanceta
4: if early accounts of fimetaria and hispanica had dissimiliar, nonconcucrrent, truly absolutely differentiable from each other spore colours, where is this accounted and what are the proposed spore colours of fimetaria, and hispanica?


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Edited by smalltalk_canceled (10/27/23 08:24 PM)

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Re: Psilocybe hispanica [Re: smalltalk_canceled]
    #28520637 - 10/27/23 09:33 PM (6 months, 18 days ago)

Okay I did a little digging and found out my friend from Germany got his print from FSE also, who got their spores from VL, who got his spores from captain future! So what I have in the above pictures is also the same as captain future and VL and anyone else who has spores sourced from there.

https://www.freespore.com/psilocybe-hispanica.html

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Re: Psilocybe hispanica [Re: Inocybe]
    #28521875 - 10/29/23 12:53 AM (6 months, 16 days ago)

Quote:

All that said, i had samples from my grow sequenced and results in genbank apparently matched it with one of the P. fimetaria  sequence uploaded to genbank. So perhaps the "hispanica" i grew is just P. fimetaria and either there was a misidentification and the true hispanica keeps lost and waiting to be found again or it is just that both are synonyms?




I think Psilocybe hispanica and Psilocybe fimetaria are the same thing, and Psilocybe fimetaria was published first so that is the name I use.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #28522387 - 10/29/23 01:44 PM (6 months, 16 days ago)

Who has noticed bruising on their fimetaria/hispanica ?? I picked some and got some great pics of bluing that happened within minutes of handling


Edited by fireinyoface (10/29/23 09:15 PM)

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Re: Psilocybe hispanica [Re: fireinyoface]
    #28523083 - 10/30/23 07:10 AM (6 months, 15 days ago)

Nice bluing! Am I right in thinking you also posted this to r/fimetaria?

Some people's cultivated P.fimetaria does blue strongly like yours do. Not all, however.


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Re: Psilocybe hispanica [Re: smalltalk_canceled] * 1
    #28523086 - 10/30/23 07:12 AM (6 months, 15 days ago)

I wouldn't focus too much on varying spore colours of closely related Psilocybes. The differences,if there are any, will likely be minute and in the subjective range. Remember a highly concentrated/dense spore deposit may appear darker than a spore deposit consisting of less spores.


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Re: Psilocybe hispanica [Re: fireinyoface]
    #28525557 - 11/01/23 01:56 PM (6 months, 13 days ago)

Quote:

fireinyoface said:
Who has noticed bruising on their fimetaria/hispanica ?? I picked some and got some great pics of bluing that happened within minutes of handling






Do you have a log? What was the substrate, what type of isolation was this?


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Re: Psilocybe hispanica [Re: DH42] * 1
    #28525561 - 11/01/23 02:07 PM (6 months, 13 days ago)

These from the plastic container and glass jar have no bruising




These from the basket all have bruising


Edited by fireinyoface (11/01/23 02:54 PM)

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Re: Psilocybe hispanica [Re: smalltalk_canceled]
    #28525565 - 11/01/23 02:10 PM (6 months, 13 days ago)

No log and it was just a different culture from a different plate but same original print. Going to be trying to determine if it was genetic or environmental, but I would assume there were would be more bruising on wild specimens if it was environmental. I think it's genetics personally but I'll keep you all posted

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Re: Psilocybe hispanica [Re: fireinyoface]
    #28525567 - 11/01/23 02:11 PM (6 months, 13 days ago)

If you think its genetics i will buy you a set of miraculix test. Or get one yourself!


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Re: Psilocybe hispanica [Re: smalltalk_canceled]
    #28525574 - 11/01/23 02:21 PM (6 months, 13 days ago)

Shoot me a link to something legit I'd like to have that resource!

I say I think it's genetic because they are all in similar substrates outside in similar conditions and these are so obviously different. Also I imagine their natural environment is the same if not better than what I have going right now.

I'm cloning the basket culture to see if I can replicate it in different settings. I'm hoping to get to the bottom of the environment vs genetics question

Edited by fireinyoface (11/01/23 08:16 PM)

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Re: Psilocybe hispanica [Re: fireinyoface] * 1
    #28527002 - 11/02/23 07:18 PM (6 months, 12 days ago)

Quote:

fireinyoface said:
Shoot me a link to something legit I'd like to have that resource!





The Miraculix tests are available at https://www.miraculix-lab.de/en; Note that there is a link to another site to order them in the US.  They are accurate to within about 10%, which is less accurate than HPLC but mushrooms differ from one mushroom to the next more than 10% so the accuracy is fine.

Best place to send them for DNA barcoding to confirm the species is https://docs.google.com/document/d/1nSKlouFy1Z3OXbCf2cWiKH0hkDnFKZvPn6_oCr3vEK0/edit

It's free, though he does accept donations.

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Re: Psilocybe hispanica [Re: Alan Rockefeller] * 1
    #28527032 - 11/02/23 07:38 PM (6 months, 12 days ago)

:takingnotes:

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #28528949 - 11/04/23 11:11 AM (6 months, 10 days ago)

Thanks Alan! I'm going to order some of those tests. I'd also love to send in samples for sequencing but I had always wondered if there was any legal risk with something like that? Is there anything to worry about when sending psilocybe through the mail, or is it safe because it's headed to a lab?

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Re: Psilocybe hispanica [Re: fireinyoface] * 1
    #28529774 - 11/05/23 12:14 AM (6 months, 9 days ago)

Quote:

fireinyoface said:
Thanks Alan! I'm going to order some of those tests. I'd also love to send in samples for sequencing but I had always wondered if there was any legal risk with something like that? Is there anything to worry about when sending psilocybe through the mail, or is it safe because it's headed to a lab?





There is legal risk but not much.  Mail cops only care about large amounts.  If you are sending ten pounds you should worry, but no one cares about a couple of grams.

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Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #28530572 - 11/05/23 12:38 PM (6 months, 9 days ago)



From the outdoor "Hispanica"

For another round


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Edited by smalltalk_canceled (11/05/23 12:38 PM)

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