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Re: Psilocybe hispanica [Re: Alan Rockefeller] * 2
    #27732067 -

Does a spore print is enough material? feel free to pm..

Psilocybe hispanica



hispanica printing

Those are the last pic I could upload, normal account limit reached :frown:


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Edited by V.L (04/13/22 06:53 AM)

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L]
    #27732687 -

Yes a spore print is good.    I sent you a PM and gave you unlimited image uploads.

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27732789 -

Cool! Thanks for pictures space, deleted few old pics today to upload but that’s really better! Will send a print to your way, if it can help to have more knowledge on them..


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L]
    #27752867 -

Took 2 weeks for a little second flush:



Some spores under my very old microscope and phone photo:



--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L]
    #27753944 -

Are those dry spores?

How are you preparing the slide?

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27755057 -

It was dry spores on slide then just one drop of water to separate them a bit and leave it until slide was dry again to not put water on the microscope.. I’m not practicing microscopy very often & was my first time with this specie. Did you received the print yet?


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Edited by V.L (04/28/22 01:41 PM)

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L] * 1
    #27755634 -

It hasn't arrived yet unless it was in the little stack of mail that I brought to the lab the other day. 

Spores are generally studied when hydrated - yours look a bit collapsed, which is what they are like when they are dry.

I suggest rehydrating them with a half drop of 5% KOH, then putting down a coverslip, lifting it up slightly and putting it back down until air bubbles are gone.  Optionally paint a ring of nail polish around the edge of the coverslip.    Observe using the 100x oil immersion objective, with a drop of immersion oil on top of the coverslip.  If you don't have immersion oil you can use vegetable oil or even isopropanol in a pinch.

If you don't have KOH you can use 70% isopropanol, then let it half dry by blowing on it for 20 seconds or so, then add a half drop of water and put down the coverslip.

If you have a condenser under the stage, open and close it until the image looks best.  It generally should be more closed with the lower power objectives and more open at the higher magnifications.

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27756033 -

Thanks for all the info & advice about microscopic practice it’s an other world!! even I’m usually more into macroscopic observations and printing. I did a 2nd cake from more isolated culture and it’s pinning like crazy! Will share some pics if they mature well.. have a nice day


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L] * 10
    #27762299 -

2nd cake 1st flush


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: Alan Rockefeller] * 1
    #27762497 -

I agree Alan, the ones in this thread do look similar to P.fimetaria. I'll attach some examples of hispanica-looking-fimetaria below.

If P.hispanica is actually P.fimetaria, it would mean we would be able to add sheep manure to the list of substrate from which P.fimetaria grows. Also, I have a suspicion that P.fimetaria might grow from Red Deer manure - at least in Scotland/UK.

I saw in another comment, from a few years ago, you said you had an ITS and LSU sequence of P.hispanica. Have you tried comparing the ITS to one of P.fimetaria?














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Have a look at the subreddit r/fimetaria!

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: DH42]
    #27762612 -

Yes maybe they are conspecific with fimetaria (I don’t think with semilanceata) I hope Alan will receive the prints and could give the final answer with genome sequencing.. personally really love how they look! hispanica or even if it’s same with fimetaria.. do you or someone have a fimetaria prints? so I can work also on it to try to do a comparative grow. And does anyone know a bit more about hispanica/fimetaria usual potency?


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L] * 6
    #27762754 -

I think Psilocybe alutacea should be included in these comparisons.


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Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L] * 1
    #27765544 -

V.L said:
Yes maybe they are conspecific with fimetaria (I don’t think with semilanceata) I hope Alan will receive the prints and could give the final answer with genome sequencing.. personally really love how they look! hispanica or even if it’s same with fimetaria.. do you or someone have a fimetaria prints? so I can work also on it to try to do a comparative grow. And does anyone know a bit more about hispanica/fimetaria usual potency?



Perhaps they are conspecific with P.fimetaria. You are right - they are definitely different to P.semilanceata. However, they are undoubtedly closely related.

I do have some P.fimetaria prints.

Potency for P.fimetaria (and perhaps, by extension, P.hispanica) is an unresolved topic. From what I have observed and been told, the potency varies considerably for P.fimetaria. Most often it is considerably less potent than, say, P.semilanceata. However, some people report effects on par with P.semilanceata gram-for-gram. The issue ties in with the (lack of) blue bruising found on most P.fimetaria specimens. I believe it might be something to do with differing substrate. I think cow manure produces weak specimens whereas horse manure can produce more moderate ones. However, the existence of P.liniformans confuses things considerably. It looks practically identical to P.fimetaria and the only differing macroscopic feature is the presence of a separable gelatinous gill edge. So, I believe some of the people who have reported strong trips off P.fimetaria *might* have been actually consuming P.liniformans. More work is needed!


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Have a look at the subreddit r/fimetaria!

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: inski] * 3
    #27765546 -

inski said:
I think Psilocybe alutacea should be included in these comparisons.




Another potential candidate for P.fimetaria!



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Have a look at the subreddit r/fimetaria!

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: DH42] * 1
    #27766754 -

Thanks Insky & DH42 interesting! I only had few fruits on my alutacea attempt years ago and last tries it didn’t germinate (probably too old) Feel free to PM to exchange few prints ; ) to try to compare all of them.. have a beautiful weekend!


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: V.L] * 2
    #27776389 -

Hey just popping by to post some photos of a few fruits from this years semilanceata grow.
I will send these away for sequencing soon and will post the result.



--------------------
🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

Edited by Baba Yaga (06/15/22 03:41 PM)

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: Baba Yaga]
    #27779682 -

Baba Yaga said:
Hey just popping by to post some photos of a few fruits from this years semilanceata grow.
I will send these away for sequencing soon and will post the result.


Very cool!  When you get data PM me if you want to do a quick zoom call - I can share my screen show you how I analyze sequence data using free tools and put it into Genbank.

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: Alan Rockefeller]
    #27779822 -

That would be great Alan.


--------------------
🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: Baba Yaga]
    #27780540 -

very nice - on the assumption that they are growing from dung (which it looks like they are) they may well be P.fimetaria!


--------------------
Have a look at the subreddit r/fimetaria!

Extras: Filter Print Post Top
Re: Psilocybe hispanica [Re: DH42]
    #27780714 -

At this stage anything is possible. This is grain spawned to potting mix by the way,
no manure in there at least it didn't say anything about it on the bag.

Still hoping that these are semilanceata since none of the cultivated specimens
really look like what you can find in the wild.


--------------------
🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

Extras: Filter Print Post Top
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Shop: Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds

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