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Sporulator


Registered: 03/08/11
Posts: 1,643
Loc: Europe
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Quote:
Alan Rockefeller said: Could P. hispanica be conspecific with P. semilanceata?
The ITS sequences are just one base pair different, and it's just that one A turned into two A's near the beginning of the sequence, which is likely not even a real difference.
Perhaps they found P. semilanceata on horse dung and thought it was something new because of the unusual substrate. But horse dung is really just chewed up grass, so it's not that much of a leap - and P. semilanceata is found in the area.
The LSU sequences of the two species are identical.
I am having trouble finding the original species description, which was published in "Docums Mycol. 29(no. 116): 42 (2000)". Docums Mycol. is short for Les Documents Mycologiques.
Here's a photo of the holotype: http://mushroomobserver.org/241329
I never saw Psilocybe semilanceata fruiting directly from cow patties or horse droppings.
The Psilocybe hispanica strain I am working with does not look like Psilocybe semilanceata.
It is easy to grow compared to Psilocybe semilanceata, a casing layer is not necessary. It is a cold weather species like Psilocybe semilanceata, fruiting between 40 and 60 F and the potency is definitely equal to Psilocybe semilanceata.
But is the species I am working with really Psilocybe hispanica?
And was the material used for DNA sequencing really Psilocybe hispanica? Where did the material come from? This is all very confusing.

Edited by Sporulator (01/03/18 10:32 AM)
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Sporulator


Registered: 03/08/11
Posts: 1,643
Loc: Europe
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Re: Psilocybe hispanica [Re: Mateo] 1
#24893131 - 01/05/18 11:04 PM (6 years, 23 days ago) |
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Quote:
Mateo said: I saw pictures of a whole cluster P.Semilanceata fruiting directly from some animal poo. It was a thread about it in a swedish forum back in 2012. And it was Psilocybe semilanceata, im 100% sure of it having picked thousands of them. We thought it was odd it fruiting directly on the poo like that.
In fact i have always thought P.Semilanceata has some connection to dung. Where they grow in suburb areas over here they tend to grow grass lawns who has been newly laid out or grass lawn sown previous year. Then they grow on these for a few years with declining numbers. Just if they use up the nutrients or fertilizers in the soil. And they always use animal poo of some sort as a fertilizer. So P.Semilanceata is probably a dung lover, at least partially.
I agree with you that Psilocybe semilanceata is a nitrophilous species.
But I've never found them fruiting directly from dung here in Central Europe.
I always find them growing on former and now abandoned pastures, which have turned into ordinary grassland.
I suspect that another active species from Northern Spain could be involved in this confusion.
Psilocybe gallaeciae described in 2003 for the first time by Guzman in this paper:
http://www.samorini.it/doc1/alt_aut/ek/guzman03.pdf
http://mushroomobserver.org/observer/show_observation/59103
Edited by Sporulator (01/07/18 09:22 AM)
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Sporulator


Registered: 03/08/11
Posts: 1,643
Loc: Europe
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Re: Psilocybe hispanica [Re: Mateo] 1
#24895451 - 01/06/18 11:13 PM (6 years, 22 days ago) |
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Quote:
Mateo said: How much must a mushroom change/evolve to be considered a new one?
Good question! But I'm a cultivator, not a taxonomist. You should ask Alan Rockefeller or Workman this question.
I think an interesting experiment would be to grow mycelium of Psilocybe semilanceata and Psilocybe hispanica on the same agar plate.
If they are the same species, no barrage formation should occur. Barrage formation means, that no nuclear exchange takes place, although hyphal fusions occur.
https://link.springer.com/chapter/10.1007/978-3-642-69299-4_17
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Sporulator


Registered: 03/08/11
Posts: 1,643
Loc: Europe
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Re: Psilocybe hispanica [Re: Mateo] 1
#24895921 - 01/07/18 08:34 AM (6 years, 22 days ago) |
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Quote:
Mateo said: So if for example a mycel of say Psilocybe Cubensis variation Maztapec were grown on the same agarplate as Psilocybe Cubensis variation Golden teacher, the two would not form a barrier when they meet and mix smoothly as both are Psilocybe Cubensis?
Yes.
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