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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Workman]
    #24205371 - 03/30/17 01:13 PM (6 years, 9 months ago)

Could P. hispanica be conspecific with P. semilanceata?

The ITS sequences are just one base pair different, and it's just that one A turned into two A's near the beginning of the sequence, which is likely not even a real difference.

Perhaps they found P. semilanceata on horse dung and thought it was something new because of the unusual substrate.  But horse dung is really just chewed up grass, so it's not that much of a leap - and P. semilanceata is found in the area.

The LSU sequences of the two species are identical.

I am having trouble finding the original species description, which was published in "Docums Mycol. 29(no. 116): 42 (2000)".  Docums Mycol. is short for Les Documents Mycologiques.

Here's a photo of the holotype:  http://mushroomobserver.org/241329


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Workman]
    #24206168 - 03/30/17 06:12 PM (6 years, 9 months ago)

Perhaps a variety that has evolved to be more tolerant of different substrates.  Whether it should be called a separate species is hard to say, I wonder what they would look like cultivated side by side.....and if there are any microscopic differences.  I wonder if the difference in ITS sequences between P. hispanica and P. semilanceata would continue to be seen if more collections were sequenced.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: funkymonk22]
    #24211120 - 04/01/17 02:50 PM (6 years, 9 months ago)

How was the potency?  One person grew P. hispanica and said that nothing happened when the fruits were eaten.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Mateo] * 1
    #24911907 - 01/13/18 08:09 PM (6 years, 15 days ago)

Quote:

Mateo said:
How much must a mushroom change/evolve to be considered a new one?





There is a central authority on nomenclature, but no central authority on taxonomy.  Therefore it is up to each individual person to answer that question for themselves, as there is no set number of changes that triggers it being new species.

I like to follow Dr. Bas's species concept, which is that there should be three unrelated differences.  They could be macromorphological, microscopic, chemical or molecular (DNA).

The biological species concept is a good way to answer this question in animals, but does not really work in plants or fungi since they hybridize often.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: MushroomOfLife] * 1
    #25284095 - 06/21/18 07:54 PM (5 years, 7 months ago)

Quote:

MushroomOfLife said:
Sorry to revisit an old thread, but Kale is the spawn of the devil!





Why?


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: V.L] * 1
    #27731885 - 04/12/22 07:28 PM (1 year, 9 months ago)

I believe that Psilocybe hispanica is a synonym of P. semilanceata or P. fimetaria.    The fruits in this thread look like P. fimetaria.

Someone should do some DNA work on these!

I'd be happy to but I don't have any material.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: V.L]
    #27732687 - 04/13/22 11:08 AM (1 year, 9 months ago)

Yes a spore print is good.    I sent you a PM and gave you unlimited image uploads.


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Re: Psilocybe hispanica [Re: V.L]
    #27753944 - 04/27/22 07:38 PM (1 year, 8 months ago)

Are those dry spores?

How are you preparing the slide?


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: V.L] * 1
    #27755634 - 04/28/22 08:03 PM (1 year, 8 months ago)

It hasn't arrived yet unless it was in the little stack of mail that I brought to the lab the other day. 

Spores are generally studied when hydrated - yours look a bit collapsed, which is what they are like when they are dry.

I suggest rehydrating them with a half drop of 5% KOH, then putting down a coverslip, lifting it up slightly and putting it back down until air bubbles are gone.  Optionally paint a ring of nail polish around the edge of the coverslip.    Observe using the 100x oil immersion objective, with a drop of immersion oil on top of the coverslip.  If you don't have immersion oil you can use vegetable oil or even isopropanol in a pinch.

If you don't have KOH you can use 70% isopropanol, then let it half dry by blowing on it for 20 seconds or so, then add a half drop of water and put down the coverslip.

If you have a condenser under the stage, open and close it until the image looks best.  It generally should be more closed with the lower power objectives and more open at the higher magnifications.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Baba Yaga]
    #27779682 - 05/16/22 12:40 AM (1 year, 8 months ago)

Quote:

Baba Yaga said:
Hey just popping by to post some photos of a few fruits from this years semilanceata grow.
I will send these away for sequencing soon and will post the result.




Very cool!  When you get data PM me if you want to do a quick zoom call - I can share my screen show you how I analyze sequence data using free tools and put it into Genbank.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Baba Yaga]
    #27886287 - 08/02/22 12:25 AM (1 year, 5 months ago)

Yes definitely semilanceata.  I replied to your PM.

It would be cool to put these photos on Mushroom Observer, upload the sequence data to Genbank and link the Genbank record to the Mushroom Observer record so there's a permanent record of this, it'd be good to be able to refer to it when looking at possible semilanceata photos.

A good way to share .ab1 files is to put it on a public Google drive link.

You can also contact me on gmail, Facebook messenger and IG, I use the same name on all 3.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: smalltalk_canceled]
    #27923995 - 08/29/22 12:09 PM (1 year, 4 months ago)

Quote:

smalltalk_canceled said:

Does this mycelium look like agaricus blazeii or like hispanica





It is hard to see what is going on here, but I would guess a mold.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: V.L]
    #27924013 - 08/29/22 12:24 PM (1 year, 4 months ago)

Quote:

V.L said:
Alan did you received/sequenced the sample I sent you?




What is the iNaturalist or Mushroom Observer observation number?


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: smalltalk_canceled] * 2
    #28495997 - 10/07/23 10:35 PM (3 months, 19 days ago)

A friend ITS sequenced Psilocybe hispanica that he got from someone (VR or something) and it was a 100% match for Psilocybe fimetaria.

I also saw a photo of P. hispanica that the person who discovered it took, and it looked just like P. fimetaria, so I think that's what these are.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Workman]
    #28497382 - 10/09/23 08:45 AM (3 months, 18 days ago)

Quote:

Workman said:
Hmmm, it does look identical. I'll get my sample tested and report back. I also have some fimentaria and liniformans to grow out and compare.





I've put P. fimetaria and P. liniformans sequences in Genbank for comparison.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: Inocybe]
    #28521875 - 10/29/23 12:53 AM (2 months, 29 days ago)

Quote:

All that said, i had samples from my grow sequenced and results in genbank apparently matched it with one of the P. fimetaria  sequence uploaded to genbank. So perhaps the "hispanica" i grew is just P. fimetaria and either there was a misidentification and the true hispanica keeps lost and waiting to be found again or it is just that both are synonyms?




I think Psilocybe hispanica and Psilocybe fimetaria are the same thing, and Psilocybe fimetaria was published first so that is the name I use.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: fireinyoface] * 1
    #28527002 - 11/02/23 07:18 PM (2 months, 24 days ago)

Quote:

fireinyoface said:
Shoot me a link to something legit I'd like to have that resource!





The Miraculix tests are available at https://www.miraculix-lab.de/en; Note that there is a link to another site to order them in the US.  They are accurate to within about 10%, which is less accurate than HPLC but mushrooms differ from one mushroom to the next more than 10% so the accuracy is fine.

Best place to send them for DNA barcoding to confirm the species is https://docs.google.com/document/d/1nSKlouFy1Z3OXbCf2cWiKH0hkDnFKZvPn6_oCr3vEK0/edit

It's free, though he does accept donations.


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OfflineAlan RockefellerM
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Re: Psilocybe hispanica [Re: fireinyoface] * 1
    #28529774 - 11/05/23 12:14 AM (2 months, 22 days ago)

Quote:

fireinyoface said:
Thanks Alan! I'm going to order some of those tests. I'd also love to send in samples for sequencing but I had always wondered if there was any legal risk with something like that? Is there anything to worry about when sending psilocybe through the mail, or is it safe because it's headed to a lab?





There is legal risk but not much.  Mail cops only care about large amounts.  If you are sending ten pounds you should worry, but no one cares about a couple of grams.


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