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Offlinenewtothis3
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Registered: 11/02/14
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self-healing ports and blunt tip syringes?
    #21020352 - 12/24/14 07:59 AM (9 years, 4 months ago)

Can you use self-healing ports on jars with blunt tipped syringes?  If not, what should I do?


Thanks!

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Invisiblewowimflabbergasted
supercalifragilistic
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Registered: 07/16/12
Posts: 18,919
Re: self-healing ports and blunt tip syringes? [Re: newtothis3]
    #21020428 - 12/24/14 08:37 AM (9 years, 4 months ago)

I would get the ones that aren't blunt....


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OfflineMonkeyJesusFresco
am i suspended in agar?
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Registered: 10/09/12
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Re: self-healing ports and blunt tip syringes? [Re: wowimflabbergasted]
    #21020463 - 12/24/14 08:50 AM (9 years, 4 months ago)

hmm... I wouldn't.

If you're going to be inoculating substrate cakes (like pf cakes) then you should be fine using a still air box and just being mindful that the dry verm layer remains intact.

If you're planning on inoculating grain jars, then just use a still air box and crack the lid a little, slide the needle in and drip drip drip or whatever...

However, if you are feeling REALLY uncomfortable about NOT using a self-healing injection port then what I do (mostly to put my mind at ease):

Unscrew the bands on the grain jars, making sure the lids stay 'sealed' and don't pop up for any reason;
blast the tops of the jars with lysol, and place however many jars you can comfortably work with inside your SAB.
Let the air settle in the box (wait 15-20mins) and then get to work.

The idea is that the lysol will kill/neutralize any bacterials on the jars themselves, use clean gloves and clean the needle tip (flame it outside of the SAB) and standard SAB procedure should neutralize air-borne contaminating spores....

also it should be noted, the lysol thing, it's very possible it's a placebo effect, but hey, it puts my mind at ease :super:

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