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InvisibleLanaV
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Registered: 10/28/99
Posts: 3,078
Loc: www.MycoSupply.com
Gentamicin Sulfate?
    #2101281 - 11/13/03 12:58 PM (13 years, 26 days ago)

So my cousins boyfriend sent me a link about gentamicin sulfate.

I read it over and I think I'm missing something....  Look here - http://www.kitchenculturekit.com/Mushroom/agarplates.htm scroll 3/4 of the way down.  Step 9 says "Allow media to cool until mixture is warm to touch, however, not solidified. Add 0.5 cc of Gentamicin Sulfate per 10 cc of solution (1.5 cc per cup)"

Now, maybe my math is off but, my other cousins boyfriend uses 0.100 grams of gentamicin sulfate per 1,000ml (one liter) of water.

500ml = 1/2 liter
500ml = 2 cups

You do the math?

Is the first link really off in their calculation?  It seem like they're using A LOT of gentamicin sulfate in da mix :smile:

Lana
 


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Invisiblemicro
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Re: Gentamicin Sulfate? [Re: Lana]
    #2101336 - 11/13/03 01:13 PM (13 years, 26 days ago)

Depends on how dilute the gentamicin solution is. Ususally it's waaay more concentrated, though, and you add something like 1 ml/L.

They might be selling a really dilute solution with the kit, but they still have their head stuck up their ass because you don't need the media to cool -- gentamicin is autoclavable. Just adds another contamination vector.

--
Micro


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Invisibleshroomophile
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Re: Gentamicin Sulfate? [Re: Lana]
    #2102624 - 11/13/03 07:11 PM (13 years, 26 days ago)

My gent.sulfate is 100 mg/ml. I use 1 ml per liter.
Micro is right,add it before you autoclave.


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Offlinebonsai
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Re: Gentamicin Sulfate? [Re: shroomophile]
    #2104187 - 11/14/03 01:37 AM (13 years, 25 days ago)

My gentamycin is in 1 ml ampoule and contains 40mg. I use one for one liter. Am I using too much dilution?
bonsai


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OfflineAnnoA
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Re: Gentamicin Sulfate? [Re: bonsai]
    #2104448 - 11/14/03 02:46 AM (13 years, 25 days ago)

Generally one uses 100 mg per liter
You use 40.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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