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icemanx
biochemist


Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: mushpunx]
#21011535 - 12/22/14 09:58 AM (9 years, 4 months ago) |
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when do i know when its time to transfer? like im starting to get white spots in the clear parts, when the spots are all white? or like now when they are starting to turn white? what do you think? they look healthy right?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#21011538 - 12/22/14 09:59 AM (9 years, 4 months ago) |
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How many drops did you put on that plate?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist


Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#21011560 - 12/22/14 10:06 AM (9 years, 4 months ago) |
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they are all different plates, but it was 2, i did 1 on each side.... im actually a little confused why it seems to be more spread out like multiple drops in an area but it was only 2 drops
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#21011572 - 12/22/14 10:10 AM (9 years, 4 months ago) |
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'Cause it slides around or your drops explode when they hit agar depending on the height.
Looking at the third pic I see mic growth in those small patches around 6 and 7 o'clock. Can you take a better picture and put it up? What strain?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist


Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#21011585 - 12/22/14 10:14 AM (9 years, 4 months ago) |
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ill try to get some better pics, its a little hard right now with everyone home, but i have blue meanies and pink buffalos
they look healthy so far though right? nothing to worry about? when should i transfer them? can i use pieces from these plates to transfer to WBS?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#21011596 - 12/22/14 10:17 AM (9 years, 4 months ago) |
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I would do another transfer before knocking up grains.
Yeah, transfer the fastest grower. Maybe I haven't used clear agar but it just looks real whispy to me.

-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist


Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#21011623 - 12/22/14 10:28 AM (9 years, 4 months ago) |
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yea im going to need to buy more agar, i have more plates just need more agar while im out of it ill pick up some food dye too. for the food dye is it added once the system is sterilized or after? im guessing before, but then how much do i add if i have 500 ml of water and agar powder?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#21011630 - 12/22/14 10:31 AM (9 years, 4 months ago) |
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Throw it in your 500 ml of water. Use 2 drops of red and 3 drops of blue for a dark purple that's perfect for seeing growth versus contams.
No matter how much food coloring you add as long as the water, agar and your nutrients are good then it'll grow. Food coloring doesn't affect myc growth.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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