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OfflineSwing
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OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's
    #21004407 - 12/20/14 06:16 PM (9 years, 4 months ago)

So I've been letting my cakes grow inside my SGFC. I've experimented with dunking, not dunking, trying minibulks (ultimate failure for another topic), and just putting the jar in my chamber with WAAAAAAY better results. Anyways what I did was open a jar, emptied the water, cold shocked it by adding new cold water in the jar, and gently pushed it into my perlite layer. As you can see in the pics (I've already harvested a couple times). There's just way more shrooms growing out of the jar then just the plain dunk and roll cakes. And it was a mistake testing a cake out without coldshock, dunking and rolling because I've only got one mushy from that. Anyways here's what I got from doing something way more simpler than dunking and rolling.


You can see as far as mushroom numbers it goes jar>dunknroll>whitecake (at least for me).

Now I got a couple questions. Considering I only harvested one mushy and only have one small one growing on my white cake, should I just dunk n roll it right? And the same for my cake in the middle which was my first which has yielded a good harvest right? That's what I would call my "second flush"? Also should I just tear off the bases of the shrooms that I cut off that are still left on that cake?

Now my major question. I definitely let this buddy sit too long.


What should I do with him? Take out the cake and set it in my SGFC? just open the lid and let em grow up and out like my last buddy? I'm confused and could use some guidance for how I should move further.

Thanks for all the expertise in advance guys, I would have never had any mushrooms by christmas if it wasn't for you!:thumbup:

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OfflinetaGyo
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Swing]
    #21004519 - 12/20/14 06:37 PM (9 years, 4 months ago)

I'd just open the lid on that second one.

You don't always get those results from in-jar. Perhaps one of the reasons why they're pinning so well in the jar is because they've consolidated the substrate and hence have directed more energy towards fruiting.

Cold-shocking is obsolete information.


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OfflineSwing
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: taGyo]
    #21005050 - 12/20/14 08:35 PM (9 years, 4 months ago)

But I thought u dunked cakes sometimes 2-3 times to get the most out of them?

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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Swing]
    #21005066 - 12/20/14 08:39 PM (9 years, 4 months ago)

Dunk...yes but only roll the first time,just a contam vector after that


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OfflineSwing
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: cronicr]
    #21005178 - 12/20/14 08:57 PM (9 years, 4 months ago)

Ok and if I open this jar these mushrooms should hopefully pop up right side up?

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InvisiblebodhisattaMDiscordReddit
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Swing]
    #21005342 - 12/20/14 09:25 PM (9 years, 4 months ago)

the results are hardly conclusive when having such a small amount of jars. your results are from the noise not from what you're doing to them differently. you likely would have got the same varying results had you put the cakes in all the exact same way.

cold shocking is pointless for cubensis they're tropical

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InvisibleFuzz-nutter
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: bodhisatta]
    #21010943 - 12/22/14 04:22 AM (9 years, 4 months ago)

Your SGFC is the issue. Jars are doing better because the fruiting chamber is not optimal.

:havesomescience:

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InvisibleViolet
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Swing]
    #21011304 - 12/22/14 08:31 AM (9 years, 4 months ago)

Quote:

Swing said:




Lovely.  Personally I think pictures like this can speak volumes.
I do agree that maybe your SGFC needs checking out to make sure it's proper, but if it isn't it still vouches that fruiting from the jar is more hardy in a variety of conditions.

You pretty much have a cased substrate in there.  The cake fruited from the jar has not been handled and has still been given more water, then is put in fruiting conditions with no more substrate exposed to environs than necessary.  They keep their moisture better and probably have a better fruiting condition up top than the other cakes have at most any point.

I know for sure if I took my straight-grain cakes out of their containers and set them straight into a sgfc the results would be much more poor than I get leaving them in their containers.  Then again those straight-grains do kindof need a casing layer for a couple reasons, yet I still think the difference applies to other grows like this.

Are those quarter-pint jars?  If you were using the tall half-pint jars, you'd have twice as much substrate but the same amount of fruiting area.  Would look pretty much like these do.

Not sure if I have seen a direct comparison of birthed versus jar-fruited PF cakes.  Would like to.


--------------------
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OfflineSwing
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Violet]
    #21012459 - 12/22/14 02:37 PM (9 years, 4 months ago)

Well there's your comparison right there!

And I take offense to you sir about your discrediting remarks on my SGFC! lol Actually it's exactly upto date as per instruction 4-5 inches perlite, 66 qtz, 2-2.5 inch apart qtr inch holes. Actually the small mini SGFU I made to start them out with wasn't giving me great results until Tayo told me to go make my proper SGFC to EXACT specifications. Next day, I had many new mushrooms and my slow growing ones grew 3 inches in one night!

Anyways I do have a 1/2 pint jar I just put waaaaayyy too much verm, my syringe couldn't make it past verm layer and I used too much just so it would drip down the top, it's taken about a month for it to creep down it's about 2/3 colonized.

I do realize now that you need proper FAE and the reason for so much perlite was to keep a continuous evaporating humidity rising up.

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InvisibleGuardian187
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Swing]
    #21012542 - 12/22/14 03:02 PM (9 years, 4 months ago)

I had better results when I trimmed my foil to fit the cake and also placing a humidifier in my room. My ambient humidity was below 20% (winter). Super dry! And I also added more moist perlite to hit 6" -- that helped too!

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InvisibleFuzz-nutter
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Guardian187]
    #21013199 - 12/22/14 05:27 PM (9 years, 4 months ago)

Is that picture your old fruiting chamber then? because those holes are a good 4-6 inches apart. Might not be as big a deal above ground, but if you don't have the proper holes below the perlite it wont allow enough air to move through it.

Also, stack your cake one over the other to reduce some drying on the top, and move your cakes closer together to create an ideal micro-climate between each other.

C'mon swing get your shit in order. Look at my signature, does that look like a cake that would have done better in a jar? where would they have all grown from?

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OfflineSwing
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Fuzz-nutter]
    #21013350 - 12/22/14 05:50 PM (9 years, 4 months ago)

Jesus man my cakes grow on the bottoms and corners. How did that cake end up not getting verm/perlite all over it or end up smashing into other mushies or walls? Just how the hell you get that? Yeah my fruiting chamber is up to par all holes 2-2.5 inches apart same bin as the video everything.

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Offlineblackout
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Re: OMG in-vitro (or just growing out of jars) in SGFC is 100x better! (pics) Also some Q's [Re: Swing]
    #21014132 - 12/22/14 08:38 PM (9 years, 4 months ago)

Quote:

Swing said:
Ok and if I open this jar these mushrooms should hopefully pop up right side up?



Or you could leave it in the jar to grow. If doing this you should turn your jar upside down, for better air exchange.

Do you realise invitro growing is a "tek" popularised by a user called Hippie3. It sort of was frowned upon, here is an old thread where RR showed his ones.

http://www.shroomery.org/forums/showflat.php/Number/5711989/fpart/all/vc/1
Quote:

RogerRabbit said:
Here's a few of mine. A good, isolated invitro strain will begin pinning within a week of full colonization, and look like the jars below two to three weeks after that, even on straight rye berries.  With multispore inoculation, getting a good invitro performer is going to take a lot of misses before a good hit.  Be sure to clone the fruits when you find a good one.  You can't get more stealth than invitro and they're great for having freshies whenever you want.
RR





http://www.shroomery.org/forums/showflat.php/Number/6007312
Quote:

Hippie3 said:
pictures speak louder than words
so listen up-


now show me cake that gets 10x as much as those...



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