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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Mrbarts easy no pour agar
#21008861 - 12/21/14 06:30 PM (9 years, 4 months ago) |
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Now im going to start off by saying this is how i get shit done when making agar . This tek will you a nice nutritious firm agar dish .
>To start off you need wide mouth half pints. The 1/2 pint elites work great too. Use the plastic lids as well makes it mush easier when doing agar work .

>Now you will need two things .

>Agar which can be bought online or at stores. Mexican , health and oriental stores seem to carry agar . When buying agar get the agar with no sugar .
There are many agar recipes but im doing a potato dextrose agar . which will need to include Idahoan potato flakes .
(optionally yeast can be added to the mix)
>To prepair the mix you want

6 grams potato flakes.

9 grams agar powder

1 gram yeast (optionally)
>Mix your dry ingredients into a small pan and add 500ml cold water and mix well.


>Now put the mix on the stove and bring it to a boil while stiring constantly . now add 4 teaspoons (10ml) of light karo syrup or honey.

Let it boil for a few minutes then take it off the stove and let it cool for a minute. it should be thinker than it was . More of a gloopy consistency

>take the mix and fill your jars about a quarter inch full

>Once filled screw the lids on and put in pc and cook at 15psi for 35 mins .

>Once down turn the stove off and let pc cool overnite .
You are now ready to begin your career as a mad scientist
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21008875 - 12/21/14 06:34 PM (9 years, 4 months ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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Re: Mrbarts easy no pour agar [Re: cronicr]
#21008901 - 12/21/14 06:40 PM (9 years, 4 months ago) |
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Short and sweet. Nice write up!
-------------------- “Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.” ― George Orwell, 1984 "If you can't explain it simply, you don't understand it well enough." ― Albert Einstein
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jessepinkman
Stranger


Registered: 09/28/14
Posts: 308
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About how many plates, or jars did this make for you?
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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7 qt inch agar jars. Recipe can be used in ratio of course.
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21017630 - 12/23/14 03:23 PM (9 years, 4 months ago) |
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Thinking about this further. Shouldn't there be a vent hole in the lid to prevent boil over of the agar, and allow built-up pressure to escape while in the pressure cooker?
Edit: Not to mention that the mycelium needs the gas exchange while growing as well, wouldn't it stall out using a sealed lid?
-------------------- “Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.” ― George Orwell, 1984 "If you can't explain it simply, you don't understand it well enough." ― Albert Einstein
Edited by Fungus Mountain (12/23/14 03:29 PM)
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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It wont over boil .
Theres plenty of oxygen on the jars healty mycelum . Works perfect for me and everyone who uses this tek .
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Edited by mrbart4444 (12/23/14 03:33 PM)
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,483
Loc: illinois
Last seen: 7 hours, 9 minutes
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21017771 - 12/23/14 03:57 PM (9 years, 4 months ago) |
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nice
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Dilated
KB Cubensis Fanaticus



Registered: 03/28/14
Posts: 2,347
Loc: The Ether
Last seen: 3 years, 6 months
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Re: Mrbarts easy no pour agar [Re: tripdawg420]
#21017805 - 12/23/14 04:06 PM (9 years, 4 months ago) |
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Isn't it hard to see growth with those lids on there? You have to watch growth from the sides yes? I'm having a hard time figuring out how you're preventing boil over as well. You don't cover how you're stacking the jars inside the PC or how much water you're putting in there.
There's a reason why if you're sterilizing a whiskey bottle/flask of agar, you fill the water quite high to prevent boil over. Maybe you could shed some light on this. I really hate pouring agar but I do it weekly. I'm
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Mrbarts easy no pour agar [Re: Dilated]
#21017887 - 12/23/14 04:24 PM (9 years, 4 months ago) |
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Bringing the pressure up very slowly is the best way to mitigate boil over with no pour agar.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Dilated
KB Cubensis Fanaticus



Registered: 03/28/14
Posts: 2,347
Loc: The Ether
Last seen: 3 years, 6 months
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Re: Mrbarts easy no pour agar [Re: NumeroEno]
#21017900 - 12/23/14 04:27 PM (9 years, 4 months ago) |
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Quote:
EnoRoxxx said: Bringing the pressure up very slowly is the best way to mitigate boil over with no pour agar.
Ya I know that, you also want to bring it down slowly. I guess I'm just going to have to experiment.
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Dilated
KB Cubensis Fanaticus



Registered: 03/28/14
Posts: 2,347
Loc: The Ether
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Re: Mrbarts easy no pour agar [Re: Dilated]
#21017917 - 12/23/14 04:30 PM (9 years, 4 months ago) |
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If the top is screwed all the way on it won't boil over but don't you run the risk of it breaking? Otherwise what's the point of having a gas exchange on your whiskey bottle? Even MudaFuka leaves his jar unscrewed slightly when sterilizing his liquid inoculant jars in the PC.
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Mrbarts easy no pour agar [Re: Dilated]
#21017935 - 12/23/14 04:37 PM (9 years, 4 months ago) |
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the lids arent that air tight and i turn my stove on high and build to 15 psi you can see through it a litte . you can use a LMEA recipe to see through it
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Dilated
KB Cubensis Fanaticus



Registered: 03/28/14
Posts: 2,347
Loc: The Ether
Last seen: 3 years, 6 months
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21017948 - 12/23/14 04:40 PM (9 years, 4 months ago) |
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Quote:
mrbart4444 said: the lids arent that air tight and i turn my stove on high and build to 15 psi you can see through it a litte . you can use a LMEA recipe to see through it
If they aren't "that air tight" then aren't you inviting contams?
I'm not talking abut seeing through the agar.. im talking about seeing through the top of the jar to watch the myc grow and spot satelites. You have to look through the side of those jars to visually spot the top of the agar media.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,867
Loc: Canada
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21017951 - 12/23/14 04:40 PM (9 years, 4 months ago) |
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A grain jar lid would also work. I actually use those jars with some agar in them to test my grain jar lids and make sure they are up to snuff 
While I find the fancy jelly jars a bit heavy and harder to see through fact is that they will work fine for agar. ProfessorPinhead also has a no pour tek that calls for them. Good writeup OP
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 18,264
Loc: PNW
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21017960 - 12/23/14 04:43 PM (9 years, 4 months ago) |
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I do this same tek but with silicone injection ports and a silicone tyvek filter. Always has worked fine for me with no boil over. I raise my jars up a bit by using some jars with water in them and make a triangle or a square with them and set the riser plate that would normally be on the bottom of the pc ontop of the jars.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Mrbarts easy no pour agar [Re: Pastywhyte]
#21017998 - 12/23/14 04:56 PM (9 years, 4 months ago) |
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Thanks guys. I dont really know what make this work the way it does but ive had success and you might as well
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21018037 - 12/23/14 05:05 PM (9 years, 4 months ago) |
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I pour a few hundred plates a week, doing 20 at home isn't a big deal.
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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Re: Mrbarts easy no pour agar [Re: mrbart4444]
#21018056 - 12/23/14 05:10 PM (9 years, 4 months ago) |
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We need to start a "GoFundMe" campaign to create see-thru mason jar lids.
-------------------- “Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.” ― George Orwell, 1984 "If you can't explain it simply, you don't understand it well enough." ― Albert Einstein
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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I made some plates in half pint PF jars with grain jar lids yesterday. Eventually I'm gonna have to get into pour agar. It just seems like it would be a real pain to pour plates inside a SAB.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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