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OfflineJollyrodger
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Premade brf cake question
    #21008678 - 12/21/14 05:59 PM (9 years, 4 months ago)

So I recently got into this, and it's absolutely fascinating. I've been doing a lot of reading for the past few months, and decided I'd give it a try. I bought some premade half pint brf jars (I know lol) and of course the brf came pretty densely packed. I probably should have opened them up to make it fluffy again and re-sterilize it but I didn't know it would be a problem yet.

So anyways I only inoculated one jar because I have to be stealthy for about another month and I'm still figuring things out. I got my first signs of growth about 6 days after inoculation.

The growth was on the very top  where the spores landed and it was hard to see, so this was taken 2 days after that.

I was stoked.:grin:

2  days later


Then it seemed like it started to slow down. I'm sure it's because the cake is so dense.

So about 3 days later (about one week of actual colonization) I noticed a few things that might be nothing but also could be something. I'm not sure yet.

The mycelium looks a little thinner and stringier for some reason. And also in some places it almost looks like there's a few green dots but I could be mistaken.



I have some agar mix coming and ill more than likely watch the cake for a few weeks to see if it colonizes but if it stalls I think I might make a plate from this just so I'm not back at square one. I also started collecting more stuff like a pressure cooker, jars, and syringe filters to make some lids (seemed safer than holes) but I'll make my own brf jars from now on.

So I have a few questions.

Do you think the mycelium will be able to colonize completely since the cake is so dense?

I know opening the jar is the worst thing you can do, I've been reading Rodger rabbits posts. But I haven't seen much on jars like this. I also read about peroxide and how you can use it on agar plates since colonizing fungi can metabolize it. So I was wondering if I could  pull it out and crumble it up to make it fluffier and maybe put some peroxide on it to prevent contams? Probably not huh. In that case is there any hope for a dense cake to turn out?

Also does my mycelium look ok? There's a rogue fuzz spot in one pic and I hope its mycelium but I'm really not sure yet, probably too early to tell.

Sorry about the lighting!

Edited by Jollyrodger (12/21/14 06:05 PM)

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OfflinecronicrFacebook
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Re: Premade brf cake question [Re: Jollyrodger]
    #21008721 - 12/21/14 06:08 PM (9 years, 4 months ago)

That looks bacterial, no dont try remix it, if anything make your own jars and use the right ones.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisibleswatt_haze
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Re: Premade brf cake question [Re: cronicr]
    #21008727 - 12/21/14 06:09 PM (9 years, 4 months ago)

with myc growing in "spots" like in the last pic, usually means bacteria

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OfflineJollyrodger
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Re: Premade brf cake question [Re: swatt_haze]
    #21008831 - 12/21/14 06:25 PM (9 years, 4 months ago)

Wow you guys are fast. That would explain the slower growth. That little fluffy spot? I didn't know bacteria could look like that. It's in a bad spot on the jar so it's hard to get a shot but I took some better pics hopefully.
Also do those look like knots or early pinning? I know contamination can cause it to try to survive by replicating.




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OfflineJollyrodger
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Re: Premade brf cake question [Re: Jollyrodger]
    #21008837 - 12/21/14 06:26 PM (9 years, 4 months ago)

You mean the mycelium will colonized in spots where the contam isn't present and appear spotty like that?

Edited by Jollyrodger (12/21/14 06:27 PM)

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OfflinecronicrFacebook
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Re: Premade brf cake question [Re: Jollyrodger]
    #21008842 - 12/21/14 06:28 PM (9 years, 4 months ago)

U can see the myc reaching right over the pf mix...that is not good and i would say it is a loss


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OfflineJollyrodger
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Re: Premade brf cake question [Re: cronicr]
    #21008870 - 12/21/14 06:32 PM (9 years, 4 months ago)

I see. That sucks lol. I kinda figured it might be a loss though since it probably won't fully colonize. I'll probably watch it for a while and if I don't see mold I might try to take a piece and grow it on antibiotic agar lol. That way I can use live stuff to inoculate next time. Not sure yet.

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Re: Premade brf cake question [Re: Jollyrodger]
    #21008873 - 12/21/14 06:33 PM (9 years, 4 months ago)

If you still have spores put those to agar while you are at it:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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OfflineJollyrodger
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Re: Premade brf cake question [Re: cronicr]
    #21008888 - 12/21/14 06:36 PM (9 years, 4 months ago)

Will do. Thanks for your help. I'll update in a few days just  because I wanna see how it does on a dense cake.

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Invisibleswatt_haze
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Re: Premade brf cake question [Re: Jollyrodger]
    #21008903 - 12/21/14 06:40 PM (9 years, 4 months ago)

Goodluck :thumbup:

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OfflineJollyrodger
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Re: Premade brf cake question [Re: Jollyrodger]
    #21008920 - 12/21/14 06:43 PM (9 years, 4 months ago)

Unfortunately I used all my "GT" spores. I tried 4 grow bags, all of them contaminated, and one liquid culture. I recently did 2 more liquid cultures with the pc and everything so if they contam I'll be thinking the syringe might be contaminated? I nocced them in a clear bag that I sprayed with 90% alcohol, flame sterilized the needle and washed my hands and jars and bags with alcohol. They all had self healing ports. And they weren't done at the same time either so if I messed up once I don't see all of them getting contaminated. But I am a noob  and noobs make mistakes. So I guess I shouldn't be surprised.

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Invisibleswatt_haze
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Re: Premade brf cake question [Re: Jollyrodger]
    #21008988 - 12/21/14 06:58 PM (9 years, 4 months ago)

Live and learn Jollyroder.  I learn best from my mistakes. Keep at it man

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OfflineJollyrodger
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Re: Premade brf cake question [Re: swatt_haze]
    #21021854 - 12/24/14 03:13 PM (9 years, 4 months ago)

Ok so I checked on the jar today and saw that the mycelium was dying. It looked shriveled slightly. So it was pretty much a done deal. So I decided to experiment since it would be a loss anyways. I poured a little h2o2 into a clean ziplock bag and swished it around inside, put the cake into the bag and using the bag I crumbled it and mixed it up.

I was able to feel how dry they made it, and it was very very hard, so I bet the mycelium was reaching to moisture. It was definitely the reason it was dying. Also, There was only a mushroom smell in the jar, no weird smells.

So I poured more peroxide on it to remoisturize it and kill anything that might land on it during this process. I wiped the lid down with alcohol and put everything back in the jar. So we'll see what happens haha. I doubt it'll work but if it does that'd be cool.

I know peroxide kills bacteria and fungus spores but it doesn't completely kill growing fungi, and established mycelium is able to make enzymes that neutralize it. So it should be able to start to regrow after a few days as long as nothing bad survives the peroxide. And if I'm very lucky lol.

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OfflineJollyrodger
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Re: Premade brf cake question [Re: Jollyrodger]
    #21021892 - 12/24/14 03:24 PM (9 years, 4 months ago)


That's how it started looking before I decided to experiment. Figured it was dying.

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OfflineJollyrodger
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Re: Premade brf cake question [Re: Jollyrodger]
    #21039076 - 12/29/14 11:32 AM (9 years, 4 months ago)

Well I still have the bag I crumbled it in and the leftover crumbs ate growing like crazy. All I smell is mushroom. So I think it'll work. But the GE for the jars are syringe filters so It definitely doesn't have enough gas exchange hence the slow growth in the jars. So I'll get some filter discs for the future. Or maybe try 2 syringe filters on a lid. Learning.

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