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Morionhyanda
myconaut


Registered: 10/03/14
Posts: 19
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First agar attempt
#21003232 - 12/20/14 01:38 PM (9 years, 4 months ago) |
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Here are the first results using Helltick's Grocery Store Agar Tek . I found this tek to be extremely easy to use (once I constructed my SAB) and so far am contam free with happy myc fuzzing up the jars. Here's hoping the rest of the process goes as smooth!


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filthyknees
no coincidence



Registered: 03/08/13
Posts: 6,283
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great hope you have more dishes poured those look like they'll be ready for transfers in a day or so
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 8 months
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that's a LOT of satelite germination. better take some transfers quick! that 3rd one has grown out a bit too much for my liking for a 1st plate, never know what germinated in there..
I think its easier/better to just have the spores centered in 1 spot in the jar so you know where the growth is supposed to come from!
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Morionhyanda
myconaut


Registered: 10/03/14
Posts: 19
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Im still a complete noob to agar so could you please explain why transferring to different agar is important? As far as the satellite germination, these guys were inoculated using spore syringes so I don't know if that would explain it....my original plan was to let the agar colonize then create a living inoculate with sterilized water in a syringe. From there knock up some was pints before going into a mono-tub
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 8 months
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you want to transfer healthy growth to new plates to make sure you're only growing healthy cube myc.
if you just inoc and use that first plate you'll let everything that was on the print/in the syringe start to grow and that's what we're trying to evade with agar!
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filthyknees
no coincidence



Registered: 03/08/13
Posts: 6,283
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-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Morionhyanda
myconaut


Registered: 10/03/14
Posts: 19
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Hey hey thanks guys, I'll whip up some agar tonight and transfer tomorrow sometime!
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filthyknees
no coincidence



Registered: 03/08/13
Posts: 6,283
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my pleasure agar is a lot of fun I think just had a species germinate from an almost engulfing bacteria I would have wasted weeks finding out the syringe was contaminated but with agar I'm now looking at two isolated strong looking growths with a few more transfers I can be ready to inoculate my grains feels great, good luck with your project
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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