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Offlineicemanx
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agar contamination?
    #20995802 - 12/18/14 07:08 PM (9 years, 4 months ago)

Made some agar plates two days ago and starting to see some spots in places it was inoculated in. How can I tell if it's mycelium or bacteria? It's a little clear and small but it looks0 like it has branches coming out.

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Offlinesiko887
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Re: agar contamination? [Re: icemanx]
    #20995814 - 12/18/14 07:10 PM (9 years, 4 months ago)

Pics dude. Fuck I hate that.


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Offlinemushpunx
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Re: agar contamination? [Re: siko887]
    #20995836 - 12/18/14 07:16 PM (9 years, 4 months ago)

Two days is pretty fast for inoculation, at least for me

Post a pic!


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Offlineicemanx
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Re: agar contamination? [Re: mushpunx]
    #20995922 - 12/18/14 07:30 PM (9 years, 4 months ago)

Don't really have pics cuz it's so small... but yea I know 2 days is fast :/ thats y I'm concerned... it is clear but it looks like it's stringy and growing out like mycelium does. I'll try to get a pic but it's been looking fuzzy through the phone camera and I don't want to open the lid in fears of introducing contaminants.

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Offlinemushpunx
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Re: agar contamination? [Re: icemanx]
    #20995954 - 12/18/14 07:35 PM (9 years, 4 months ago)

Is it in the area you i nnoculated? Did you drop spores on or use a loop?

I usually see a clear spot before I see white myc, or when I make a transfer I can see a ring of like tiny clear threads in a circle where the myc is going to grow out. If thats what it is,you'll see myc fast

Keep an eye on it, as soon as you see myc growth transfer a piece to a new plate


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InvisiblebodhisattaMDiscordReddit
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Re: agar contamination? [Re: mushpunx]
    #20995987 - 12/18/14 07:41 PM (9 years, 4 months ago)

post pictures

Quote:

bodhisatta said:


this is cube mycelium from germinated spores on agar





This is cube mycelium after a single transfer


the second image being a close up of the distance between the top most right most cut and the leading edge of mycelium

this is cube mycelium after a few transfers and the two dishes on the far right are isolates



and if you click this link
http://www.shroomery.org/forums/usergallery.php/gallery/300678/imgpl/5/imgpp/0/folder/Hyphae
that's what the mycelium looks like at 25x magnification




or look for pictures

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Offlinemushpunx
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Re: agar contamination? [Re: bodhisatta]
    #20996031 - 12/18/14 07:49 PM (9 years, 4 months ago)

Nice pics!


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OfflinetaGyo
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Re: agar contamination? [Re: mushpunx]
    #20996138 - 12/18/14 08:10 PM (9 years, 4 months ago)

Sicko what the fuck did you do?

http://www.shroomery.org/forums/viewratings.php?type=1&usernum=242137

:derail:

Anyway back on topic:



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Offlineicemanx
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Re: agar contamination? [Re: taGyo]
    #20996745 - 12/18/14 10:16 PM (9 years, 4 months ago)

Don't really have pics to post hooefully.tomorrow morning, but I used a drop from a syringe it's actually on both plates i.made it's where I dropped the spoor solution. It is actually alot like mushpunx discribed :smile: it's clear about the size of the drop

Hopefully I'll have mycelium soon!

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Offlinesiko887
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Re: agar contamination? [Re: taGyo]
    #20996833 - 12/18/14 10:43 PM (9 years, 4 months ago)

Quote:

taGyo said:
Sicko what the fuck did you do?

http://www.shroomery.org/forums/viewratings.php?type=1&usernum=242137

:derail:

Anyway back on topic:





I may have made a slight joke about kicking my cat. But people take everything so serious. I had know idea shroomery and PETA were one and the same. I still have no regrets.

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Offlineicemanx
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Re: agar contamination? [Re: siko887]
    #20998345 - 12/19/14 10:36 AM (9 years, 4 months ago)

so i was looking at my agars and they all kinda look the same, they are developing clear dots around where i inoculated the agar. i dropped a drop from a spore syringe in 2 locations on the agar. today is day 3 and it looks like this.






i know 2-3 days time to germinate is very fast so im worried about contamination. what do you all think? i dont think the pics are very clear but i did my best.

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OfflinetaGyo
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Re: agar contamination? [Re: icemanx]
    #20998349 - 12/19/14 10:36 AM (9 years, 4 months ago)

Looks like myc,

:thumbup:


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Offlineicemanx
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Re: agar contamination? [Re: taGyo]
    #20998359 - 12/19/14 10:40 AM (9 years, 4 months ago)

:laugh: so happy my project has been stalled for about a month from non colonizing rye so glad i have something going. going to look into some wild bird seed for when these babys are grown :laugh:

i have not opened the dishes and have them sitting in my drawer wrapped in plastic thats ok right?

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OfflinetaGyo
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Re: agar contamination? [Re: icemanx]
    #20998366 - 12/19/14 10:40 AM (9 years, 4 months ago)

Yup.

If you're paying anything more then 10-20 dollars for 50 lbs you're getting ripped off in this hobby. Make sure to find a good deal!


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Offlineicemanx
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Re: agar contamination? [Re: taGyo]
    #20998370 - 12/19/14 10:42 AM (9 years, 4 months ago)

i fuckin love this forum lol you guys have been very helpful
:thumbup:

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OfflinetaGyo
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Re: agar contamination? [Re: icemanx]
    #20998374 - 12/19/14 10:42 AM (9 years, 4 months ago)

5 shrooms for you,

:super:


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Offlineicemanx
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Re: agar contamination? [Re: taGyo]
    #21002565 - 12/20/14 10:34 AM (9 years, 4 months ago)

so as an update the dish has a bunch of those little clear dots, however they arent so little anymore, but still clear.... about how long until i should have white mycelium? the dishes seem to be growing nicely, the clear indentations seem to be growing like fingers and stretching out :laugh: i's not going to need to transfer them am i?

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OfflinetaGyo
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Re: agar contamination? [Re: icemanx]
    #21002577 - 12/20/14 10:37 AM (9 years, 4 months ago)

Give it a day or two to thicken up until you can clearly see strands and then transfer.
I normally wait until I can see healthy and clear growth. You should also use food coloring in your agar. Makes it easier to distinguish contams from everything else:



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Offlineicemanx
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Re: agar contamination? [Re: taGyo]
    #21008554 - 12/21/14 05:38 PM (9 years, 4 months ago)

Starting to get little spots of puffy white mycelium :smile: pics will be posted tonight!

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Offlinemushpunx
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Re: agar contamination? [Re: icemanx]
    #21008899 - 12/21/14 06:39 PM (9 years, 4 months ago)

Quote:

icemanx said:
Starting to get little spots of puffy white mycelium :smile: pics will be posted tonight!




Nice! As soon as you see that growth make a transfer dude!


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