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icemanx
biochemist

Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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agar contamination?
#20995802 - 12/18/14 07:08 PM (9 years, 4 months ago) |
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Made some agar plates two days ago and starting to see some spots in places it was inoculated in. How can I tell if it's mycelium or bacteria? It's a little clear and small but it looks0 like it has branches coming out.
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siko887
Broke


Registered: 01/05/11
Posts: 772
Loc: Ontario, Canada
Last seen: 3 years, 7 months
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Re: agar contamination? [Re: icemanx]
#20995814 - 12/18/14 07:10 PM (9 years, 4 months ago) |
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Pics dude. Fuck I hate that.
-------------------- I don't suffer from insanity, I actually rather enjoy it.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: agar contamination? [Re: siko887]
#20995836 - 12/18/14 07:16 PM (9 years, 4 months ago) |
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Two days is pretty fast for inoculation, at least for me
Post a pic!
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 Amateur Mycologists United AMU Q&A
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icemanx
biochemist

Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: mushpunx]
#20995922 - 12/18/14 07:30 PM (9 years, 4 months ago) |
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Don't really have pics cuz it's so small... but yea I know 2 days is fast :/ thats y I'm concerned... it is clear but it looks like it's stringy and growing out like mycelium does. I'll try to get a pic but it's been looking fuzzy through the phone camera and I don't want to open the lid in fears of introducing contaminants.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: agar contamination? [Re: icemanx]
#20995954 - 12/18/14 07:35 PM (9 years, 4 months ago) |
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Is it in the area you i nnoculated? Did you drop spores on or use a loop?
I usually see a clear spot before I see white myc, or when I make a transfer I can see a ring of like tiny clear threads in a circle where the myc is going to grow out. If thats what it is,you'll see myc fast
Keep an eye on it, as soon as you see myc growth transfer a piece to a new plate
--------------------
 Amateur Mycologists United AMU Q&A
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: agar contamination? [Re: mushpunx]
#20995987 - 12/18/14 07:41 PM (9 years, 4 months ago) |
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post pictures
Quote:
bodhisatta said:
this is cube mycelium from germinated spores on agar



This is cube mycelium after a single transfer

 the second image being a close up of the distance between the top most right most cut and the leading edge of mycelium
this is cube mycelium after a few transfers and the two dishes on the far right are isolates

and if you click this link http://www.shroomery.org/forums/usergallery.php/gallery/300678/imgpl/5/imgpp/0/folder/Hyphae that's what the mycelium looks like at 25x magnification
or look for pictures
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mushpunx
Fungus Punk



Registered: 04/20/14
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Nice pics!
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 Amateur Mycologists United AMU Q&A
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: mushpunx]
#20996138 - 12/18/14 08:10 PM (9 years, 4 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist

Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#20996745 - 12/18/14 10:16 PM (9 years, 4 months ago) |
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Don't really have pics to post hooefully.tomorrow morning, but I used a drop from a syringe it's actually on both plates i.made it's where I dropped the spoor solution. It is actually alot like mushpunx discribed it's clear about the size of the drop
Hopefully I'll have mycelium soon!
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siko887
Broke


Registered: 01/05/11
Posts: 772
Loc: Ontario, Canada
Last seen: 3 years, 7 months
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Re: agar contamination? [Re: taGyo]
#20996833 - 12/18/14 10:43 PM (9 years, 4 months ago) |
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Quote:
taGyo said: Sicko what the fuck did you do?
http://www.shroomery.org/forums/viewratings.php?type=1&usernum=242137

Anyway back on topic:
    
I may have made a slight joke about kicking my cat. But people take everything so serious. I had know idea shroomery and PETA were one and the same. I still have no regrets.
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icemanx
biochemist

Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: siko887]
#20998345 - 12/19/14 10:36 AM (9 years, 4 months ago) |
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so i was looking at my agars and they all kinda look the same, they are developing clear dots around where i inoculated the agar. i dropped a drop from a spore syringe in 2 locations on the agar. today is day 3 and it looks like this.


i know 2-3 days time to germinate is very fast so im worried about contamination. what do you all think? i dont think the pics are very clear but i did my best.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#20998349 - 12/19/14 10:36 AM (9 years, 4 months ago) |
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Looks like myc,
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist

Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#20998359 - 12/19/14 10:40 AM (9 years, 4 months ago) |
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so happy my project has been stalled for about a month from non colonizing rye so glad i have something going. going to look into some wild bird seed for when these babys are grown 
i have not opened the dishes and have them sitting in my drawer wrapped in plastic thats ok right?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#20998366 - 12/19/14 10:40 AM (9 years, 4 months ago) |
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Yup.
If you're paying anything more then 10-20 dollars for 50 lbs you're getting ripped off in this hobby. Make sure to find a good deal!
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist

Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#20998370 - 12/19/14 10:42 AM (9 years, 4 months ago) |
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i fuckin love this forum lol you guys have been very helpful
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#20998374 - 12/19/14 10:42 AM (9 years, 4 months ago) |
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5 shrooms for you,
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist


Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#21002565 - 12/20/14 10:34 AM (9 years, 4 months ago) |
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so as an update the dish has a bunch of those little clear dots, however they arent so little anymore, but still clear.... about how long until i should have white mycelium? the dishes seem to be growing nicely, the clear indentations seem to be growing like fingers and stretching out i's not going to need to transfer them am i?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: agar contamination? [Re: icemanx]
#21002577 - 12/20/14 10:37 AM (9 years, 4 months ago) |
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Give it a day or two to thicken up until you can clearly see strands and then transfer. I normally wait until I can see healthy and clear growth. You should also use food coloring in your agar. Makes it easier to distinguish contams from everything else:
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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icemanx
biochemist


Registered: 11/22/14
Posts: 127
Last seen: 7 years, 2 months
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Re: agar contamination? [Re: taGyo]
#21008554 - 12/21/14 05:38 PM (9 years, 4 months ago) |
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Starting to get little spots of puffy white mycelium pics will be posted tonight!
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 17 hours
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Re: agar contamination? [Re: icemanx]
#21008899 - 12/21/14 06:39 PM (9 years, 4 months ago) |
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Quote:
icemanx said: Starting to get little spots of puffy white mycelium pics will be posted tonight!
Nice! As soon as you see that growth make a transfer dude!
--------------------
 Amateur Mycologists United AMU Q&A
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