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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
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My attempts at isolating a wild ganoderma
#20988412 - 12/17/14 09:24 AM (9 years, 4 months ago) |
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I found a wild ganoderma recently, not sure what type, I was going to try to identify it after growing it out, but I think I've isolated the mycilium. My guess is that's it's a G. Tsugae, but I don't want to say so for sure.
 This is the original clone I've made on some MEA on 12/5. You can see where I took the wedges from, as well as a few contams. This picture was taken probably 2-3 days after taking the wedges, the contaminants were barely visible at the time and some may have occurred during or afterwards even (I think the parafilm cracked at some point).
 Here is the first transfer from clone grown out, it was taken on 12/9 and this picture was taken yesterday, 12/16. I did not spot any contaminants in the process, though I'm worried this may not be the ganoderma and I've just isolated another fungus of some kind. It doesn't look unlike what I'd expect, I just haven't grown it or any wood lovers before so I don't know what differences are there, it definitely looks different than cube mycilium to me. This is on MEA mixed with a wood chip tea btw. I've started a couple of grain jars from this dish as well as making two more transfers just in case there are hidden contaminants.
Sorry if the pics are kind of blurry, all I have for a camera is my phone. Let me know if anything looks off, I'll try to keep you guys updated if there's interest.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
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Re: My attempts at isolating a wild ganoderma [Re: krypto2000]
#20989562 - 12/17/14 01:51 PM (9 years, 4 months ago) |
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Here's a pic of the original mushroom I took the clone from. I broke it open and took a small piece from the inside of each half. The other piece I took became covered in cobweb so I went with the piece above.
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sooperdooper
lazy fuck



Registered: 09/06/12
Posts: 1,251
Loc: This one place
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Re: My attempts at isolating a wild ganoderma [Re: krypto2000]
#20992672 - 12/18/14 02:40 AM (9 years, 4 months ago) |
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nicely done
-------------------- -------------------------------------------------------------------- 99.9% of what i say is a lie...the other, gibberish.
    
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
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Re: My attempts at isolating a wild ganoderma [Re: sooperdooper]
#20993281 - 12/18/14 09:30 AM (9 years, 4 months ago) |
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Thanks man. I just hope it actually is the Ganoderma and not some random mold. It grows a little faster than cube mycilium, but it also seems like a fairly aggressive and rhizomorphic strain too, and in the same vein it's not alarmingly fast, definitely not trich, cobweb, or a common mold I'm familiar with. I guess the next pics/update will be once I have some visible growth in the two grain jars I've started from that dish.
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