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OfflineHerbgrower12
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Gas exchange?
    #20979442 - 12/15/14 12:45 PM (9 years, 5 months ago)

I have just inoculated jars with gymnopilus spores,and was wondering if I should put holes in the lid. Is it needed?

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Offlinespacechildo
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Re: Gas exchange? [Re: Herbgrower12]
    #20979512 - 12/15/14 01:08 PM (9 years, 5 months ago)

:bigyesnod:

you should've done it before you PC'd the grains tho.

make a new lid and do a quick lid transfer in a SAB!

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OfflineNooner
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Re: Gas exchange? [Re: Herbgrower12]
    #20979526 - 12/15/14 01:15 PM (9 years, 5 months ago)

The holes would have been made before you innoc'd. Heres's a couple links that will show you how to make grain lids with things you probably already have. Don't forget about the kcikass search function for this site.

A world of difference from growing pot eh? :wink:


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OfflinePussyFart
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Re: Gas exchange? [Re: spacechildo]
    #20979528 - 12/15/14 01:16 PM (9 years, 5 months ago)

Quote:

spacechildo said:
:bigyesnod:

you should've done it before you PC'd the grains tho.

make a new lid and do a quick lid transfer in a SAB!



This will only work if the lids get and stay sterilized.

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Offlinespacechildo
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Re: Gas exchange? [Re: PussyFart]
    #20979538 - 12/15/14 01:18 PM (9 years, 5 months ago)

Quote:

PussyFart said:
Quote:

spacechildo said:
:bigyesnod:

you should've done it before you PC'd the grains tho.

make a new lid and do a quick lid transfer in a SAB!



This will only work if the lids get and stay sterilized.




true! pc some jars with proper lids and then do the switch.
best I can think up :shrug:

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OfflineHerbgrower12
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Re: Gas exchange? [Re: spacechildo]
    #20979719 - 12/15/14 01:55 PM (9 years, 5 months ago)

Should I put tape over them and stick a needle to create a blockage but allow oxygen?

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Offlinespacechildo
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Re: Gas exchange? [Re: Herbgrower12]
    #20979766 - 12/15/14 02:06 PM (9 years, 5 months ago)


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OfflineRosen_Rot
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Re: Gas exchange? [Re: Herbgrower12]
    #20979797 - 12/15/14 02:12 PM (9 years, 5 months ago)

I hammer two holes into the lid before filling them with substrate but then again I use these holes specifically for inoculating with a syringe. I cover with foil after.


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OfflinetaGyo
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Re: Gas exchange? [Re: Rosen_Rot]
    #20979945 - 12/15/14 02:44 PM (9 years, 5 months ago)

Don't cover your holes with foil after you inoculate please.


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OfflineRosen_Rot
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Re: Gas exchange? [Re: taGyo]
    #20982872 - 12/16/14 12:57 AM (9 years, 5 months ago)

Wouldn't that make the jars exposed to possible contams?

I don't use the EZ-Felt TEK. I found out about it after I inoculated the jars and I am just following the old methods.

The jars so far have been inoculated for 2 days now. Today is day 3 and are scheduled for a shake. Still in time to rectify.


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
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OfflinePinsetter
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Re: Gas exchange? [Re: Rosen_Rot]
    #20983273 - 12/16/14 05:58 AM (9 years, 5 months ago)

There is a reason some of the old methods are old. The guys that have been here forever tested them and found them wanting. So noobs like us don't have to make the same mistakes. In your shoes I would take off the foil and put micropore tape on the holes.

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OfflinePsyCLown89
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Re: Gas exchange? [Re: Rosen_Rot]
    #20983285 - 12/16/14 06:10 AM (9 years, 5 months ago)

Quote:

Rosen_Rot said:
Wouldn't that make the jars exposed to possible contams?

I don't use the EZ-Felt TEK. I found out about it after I inoculated the jars and I am just following the old methods.

The jars so far have been inoculated for 2 days now. Today is day 3 and are scheduled for a shake. Still in time to rectify.




If you are following the PF Tek then that is why you have the dry verm layer - to try and stop any contams from reaching the substrate.

If it is grains, then you put micropore tape over the holes or make some lids with Synthetic Filter Disks (SFD) and silicone to inoculate through.


Do not cover the holes either as taGyo said, I made that mistake at first and after I removed the foil lid layer to expose the holes my BRF cakes started to colonize a lot quicker.


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OfflinetaGyo
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Re: Gas exchange? [Re: PsyCLown89]
    #20983311 - 12/16/14 06:23 AM (9 years, 5 months ago)

Quote:

PsyCLown89 said:
If you are following the PF Tek then that is why you have the dry verm layer - to try and stop any contams from reaching the substrate.

If it is grains, then you put micropore tape over the holes you inoculated through Tyvek or make some lids with Synthetic Filter Disks (SFD) and silicone to inoculate through.

Do not cover the holes either as taGyo said, I made that mistake at first and after I removed the foil lid layer to expose the holes my BRF cakes started to colonize a lot quicker.



Fixed but :whathesaid:

You're limiting your gas exchange and thus slowing your colonization. Also, after sterilization some of the steam has crept underneath your foil. Ever picked it up and hand water drip from underneath or on top? That water is a chance of failure, a couple drops and you no longer have a dry verm layer.

Wet attracts contam.


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OfflineRosen_Rot
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Re: Gas exchange? [Re: PsyCLown89]
    #20983520 - 12/16/14 08:10 AM (9 years, 5 months ago)

I am using Grains so I'll go and get some micropore tape. This tape is sold at pharmacies right? I looked up a google image and it looks like something you would use to hold a bandage or something.


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
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B+ BONANZA

   

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OfflineRosen_Rot
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Re: Gas exchange? [Re: taGyo]
    #20983535 - 12/16/14 08:14 AM (9 years, 5 months ago)

Quote:

taGyo said:
Quote:

PsyCLown89 said:
If you are following the PF Tek then that is why you have the dry verm layer - to try and stop any contams from reaching the substrate.

If it is grains, then you put micropore tape over the holes you inoculated through Tyvek or make some lids with Synthetic Filter Disks (SFD) and silicone to inoculate through.

Do not cover the holes either as taGyo said, I made that mistake at first and after I removed the foil lid layer to expose the holes my BRF cakes started to colonize a lot quicker.



Fixed but :whathesaid:

You're limiting your gas exchange and thus slowing your colonization. Also, after sterilization some of the steam has crept underneath your foil. Ever picked it up and hand water drip from underneath or on top? That water is a chance of failure, a couple drops and you no longer have a dry verm layer.

Wet attracts contam.




When I removed the jars from the pressure there was no water dripping or condensation inside the foil. The only condensation I noticed was a little bit in the jars above the grains but I assumed that's water from the hydrated seeds.


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

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Invisiblewowimflabbergasted
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Re: Gas exchange? [Re: Rosen_Rot]
    #20983553 - 12/16/14 08:22 AM (9 years, 5 months ago)

IMO, tape doesn't have any place when doing grain jars. Here's a little reading.

Quote:

FrankHorrigan said:
Like I said, it can work but micropore tape is a crap filter even compared to polyfill or tyvek. Any moisture gets on micropore and the whole thing is compromised.
Also I never like to inoculate through something that will leave a hole that needs to be covered. Self-healing inoc ports are simple to make and much safer :thumbup:




and

http://www.shroomery.org/forums/showflat.php/Number/13642707#13642707


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Offlinespacechildo
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Re: Gas exchange? [Re: wowimflabbergasted]
    #20983760 - 12/16/14 09:34 AM (9 years, 5 months ago)

generally :whathesaid:
except I like to just crack the lid open and insert whatever inoculum thats being used.
pushing a needle through something that cant be sterilized...naaah.

micropore tape should be used double up if used, but is in general a crappy filter
and the paper like stuff doesnt like being PC'd.

you remove the foil to let the filter dry up/stay dry. moist filter = no filter.

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OfflinePsyCLown89
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Re: Gas exchange? [Re: wowimflabbergasted]
    #20983765 - 12/16/14 09:35 AM (9 years, 5 months ago)

Quote:

wowimflabbergasted said:
IMO, tape doesn't have any place when doing grain jars. Here's a little reading.

Quote:

FrankHorrigan said:
Like I said, it can work but micropore tape is a crap filter even compared to polyfill or tyvek. Any moisture gets on micropore and the whole thing is compromised.
Also I never like to inoculate through something that will leave a hole that needs to be covered. Self-healing inoc ports are simple to make and much safer :thumbup:




and

http://www.shroomery.org/forums/showflat.php/Number/13642707#13642707




Hmm, interesting.

Although I am sure it is better than nothing? I have some on my agar plates and so far so good. Although I have two layers of microspore tape actually, if it makes any difference.
Right now he just has foil covering the holes completely. Rather put some micropore tape over the holes for now - it has worked for many, may not be the best but it would be better than nothing.

I personally think SFD lids are the way to go. I just have not been able to find any SFD's locally so far :frown:

I think I need to change my avatar. :undecided:


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Invisiblewowimflabbergasted
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Re: Gas exchange? [Re: PsyCLown89]
    #20983784 - 12/16/14 09:42 AM (9 years, 5 months ago)

If there are no other options for you, then sure why not throw 2 layers of tape over the holes and see what happens. SFD's are the way to go, absolutely.

I like your avatar :shrug:


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OfflineRosen_Rot
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Re: Gas exchange? [Re: PsyCLown89]
    #20983797 - 12/16/14 09:45 AM (9 years, 5 months ago)

Well its kind of too late to change anything for now. The spore is already in the grain. I managed to find some adhesive micropore tape. Its rather thick and should work well.

The incubating chamber has a small heating element that is keeping temps stable so I shouldn't have any moisture problems.

I will apply the micro-tape tonight in an SAB and see what happens. Better some GE than none as you said.

Lol I should probably change it dude


--------------------
:sporedrop:"The internet has one rule; use or be used" - Bjeldiablo :sporedrop:
''there is no loneliness, only moments where contentment is fleeting''

SBJs "The Basics"
3iRiS9 "Cirque du freak"
B+ BONANZA

   

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