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Offlinemaniec
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PF 2 Grain TEK ? Yapp! [updated with yields] * 1
    #20970502 - 12/13/14 09:53 AM (9 years, 5 months ago)

After a lot of experimentation and modification, finally made a PF2G method that works for me. I had to do this cause my grainjars gets contimated almost everytime, when I used MS syringe and agar is still far away (I have SAB and my petri dishes are poor).

I use this for GrainPrep and this for PF2G. I've made two modifications for PF2G. First, I don't cramble the cakes inside the SAB, I use cheese grater and do it outside, then load the glass with crumbs then cover with tinfoil. Secondly, I use 1cake to 1grain (the jar is half filled with grain), in my experiment this ratio is needed to earn fast&safely colonization. (it takes usually 3-4days to fullcol. without shaking anytime) Then we can G2G them just like the normal method, or spawn it to cococoir or something.

My first monotub is fruiting atm, the second is colonizing and I'm going to spawn the third tomorrow.

So I can grow a lot of mushies while doing experiment with agar. Then I'll skip this method and use agar2grain or GLC.


aaaaand some pictures :smile:

Right after PF2G


After 2-3days


After 4-6days


And the first monotub with 3jars:






Thanks for reading :smirk:


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Edited by maniec (12/21/14 04:33 PM)

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Invisiblewowimflabbergasted
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Re: PF 2 Grain TEK Success [Re: maniec]
    #20970515 - 12/13/14 09:55 AM (9 years, 5 months ago)

:firstladyofapproval:


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Offlinemaniec
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Re: PF 2 Grain TEK Success [Re: wowimflabbergasted]
    #20970876 - 12/13/14 11:34 AM (9 years, 5 months ago)

Im unrated, pls give me some mushies if u like it :smile:


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Invisiblewowimflabbergasted
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Re: PF 2 Grain TEK Success [Re: maniec]
    #20971430 - 12/13/14 02:07 PM (9 years, 5 months ago)

Right on. Try increasing FAE a tad. Those guys are looking a little lanky.


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Offlinemaniec
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Re: PF 2 Grain TEK Success [Re: wowimflabbergasted]
    #20971943 - 12/13/14 04:31 PM (9 years, 5 months ago)

Thanks for the advice, Im gonna loosen up the polyfill. Otherwise, is anybody there who tried pf2g?


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Edited by maniec (12/13/14 04:49 PM)

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OfflinetaGyo
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Re: PF 2 Grain TEK Success [Re: maniec]
    #20972125 - 12/13/14 05:18 PM (9 years, 5 months ago)

Yup,

I did it successfully but it came from contamed BRF jars and ended up contaming the whole project.
Uncommon for sure but not unheard of.


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Offlinemaniec
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Re: PF 2 Grain TEK Success [Re: taGyo]
    #20972155 - 12/13/14 05:27 PM (9 years, 5 months ago)

That was also happened with me, when I used low pf/grain ratio. The "original" tek says that you can make 2-3jars from one piece of cake. Since I increased the ratio, I barely get contaminated jars.


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Edited by maniec (12/13/14 05:30 PM)

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OfflinetaGyo
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Re: PF 2 Grain TEK Success [Re: maniec]
    #20972162 - 12/13/14 05:29 PM (9 years, 5 months ago)

I'll never do it again 'cause there are simpler ways to get more spawn but it was a fun experience :]


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Invisibleblindingleaf
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Re: PF 2 Grain TEK Success [Re: taGyo]
    #20972172 - 12/13/14 05:30 PM (9 years, 5 months ago)

thats nuts!
cheese grater outta left field


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Offlinemaniec
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Re: PF 2 Grain TEK Success [Re: taGyo]
    #20972213 - 12/13/14 05:40 PM (9 years, 5 months ago)

Cheese grater is the best :crazy2: Anyway, I hope to get some usable agar samples soon to begin isolating.


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OfflineBLINKfan420
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Re: PF 2 Grain TEK Success [Re: maniec]
    #20972276 - 12/13/14 05:55 PM (9 years, 5 months ago)

Im surprised they didnt solidify into one hunk of a cake inside the jar.  Good work!

BLiNK


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Invisibleeatyualive
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Re: PF 2 Grain TEK ? Yapp! [Re: maniec]
    #20972353 - 12/13/14 06:17 PM (9 years, 5 months ago)

great job i do the same thing!


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Offlinemaniec
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Re: PF 2 Grain TEK Success [Re: BLINKfan420]
    #20972442 - 12/13/14 06:43 PM (9 years, 5 months ago)

Quote:

BLINKfan420 said:
Im surprised they didnt solidify into one hunk of a cake inside the jar.  Good work!

BLiNK




I crumble the cakes right before spawning it to grain, so they have no time to solidify. But it happened to me once, when I crumbled the cake a day, and tried to spawn it on the following day it came out in one piece... I don't do this anymore :rolleyes:

aaand thanks for everybody. :tongue2:


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Re: PF 2 Grain TEK Success [Re: maniec]
    #20975093 - 12/14/14 11:59 AM (9 years, 5 months ago)

Quote:

maniec said:
That was also happened with me, when I used low pf/grain ratio. The "original" tek says that you can make 2-3jars from one piece of cake. Since I increased the ratio, I barely get contaminated jars.



i do about 10-12 jars per pf cake no issues. you will find that if you make your brf mix with about 1/2-1 cup less water, the pf cakes will break up easier with a fork. but only do that if you intend to use the pf cakes for p2g. i also don't pulverize the cakes before hand. i use a sterile fork to scrape them into each grain jar.


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Edited by eatyualive (12/14/14 12:00 PM)

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Re: PF 2 Grain TEK Success [Re: eatyualive]
    #20976459 - 12/14/14 06:00 PM (9 years, 5 months ago)

Yes I know that I had screwed up something when worked with low ratios. I tried with fork (cleaned with ethyl alcohol) inside the SAB and I used your pf recipe. Maybe I'll give it another try. I'll update this thread before harvesting tub1 and when tub2 begin to pinning (it has 2more jars compared to tub1).


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Edited by maniec (12/14/14 06:08 PM)

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Invisibleeatyualive
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Re: PF 2 Grain TEK Success [Re: maniec]
    #20976587 - 12/14/14 06:45 PM (9 years, 5 months ago)

were you only able to colonize 3 quart jars with 1 pf jar?

looking at your last picture you can definitely stretch that into more quarts. but if your happy with the speed then keep it up. i like to expand the pf jar as much as possible with grain so i can get more jars from 1. but ideally 10 is a perfect number. you can then g2g those 10 to 100 after that if you want. also i used wbs so seeing it in rye berry looks cool. the verm almost looks the same color. its kinda sandy looking.the modifications seem pretty good for SAB users as I usually use something a little different. were you pressure cooking your cheese grater?

i like the jars your using! making use of what you have available.

when you were using the fork to break up the pf cake did it crumble or was it muddy?

if it crumbled then you probably have a good moisture content for this. if it was a little muddy you might need to reduce the amount of water in the pf mix.

i try to evenly divide up the broken up mycelia into each jar. usually it does 12. ill then wait 3 days and shake on day 3. then have colonization usually day 6.


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Edited by eatyualive (12/14/14 06:52 PM)

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Offlinemushpunx
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Re: PF 2 Grain TEK Success [Re: eatyualive]
    #20976738 - 12/14/14 07:31 PM (9 years, 5 months ago)

When you touch the cake with the cheese grater, or when you take the cake outside of the still air box, arent you worried contaminants will settle onto them from the air and contam your grains?

I like your tek! But Id worry about doing that in open air


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Re: PF 2 Grain TEK Success [Re: eatyualive]
    #20978046 - 12/15/14 03:33 AM (9 years, 5 months ago)

I dont sterilize cheese grater in PC. I think I should use less water for cakes, cause its a little bit muddy.


mushpunx: I know a lot of moldspores stuck to cakes when crambling in open air, but colonization speed overcome theese contams. Once I had a jar that colonized about 3 days after pf2g. If I'll use low ratios like eatyualive, I would skip the hole openair-method, and do it inside a SAB.


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Re: PF 2 Grain TEK Success [Re: maniec]
    #20978454 - 12/15/14 08:14 AM (9 years, 5 months ago)

Yea that's thr only thing Id worry about man, when doing G2G or dropping agar into your jar, if you even let your gloved finger move over the top of an open jar it can be enough to ruin your day man. Anything that touch's anything that's going into a sterile jar needs to be sterile or the jar ceases to be sterile

G2G colonizes rapidly too but if I scooped out the grain with a spoon it'd all be over man hehe

Just food for thought!


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Re: PF 2 Grain TEK Success [Re: mushpunx]
    #20983281 - 12/16/14 06:04 AM (9 years, 5 months ago)

Looks like we have to harvest theese fruits soon :smile: I have to be very carefully, cause all of the secondflush pins developed. (and yeah I see they are a little thin and long, but I dont think FAE is the problem cause all of the tubs I made so far didnt have fae problem at all, and I use everytime Frank's Tek)



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