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hamloaf
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Registered: 12/23/09
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Rye Soak Water Liquid Culture! 3
#20970238 - 12/13/14 08:24 AM (10 years, 1 month ago) |
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Was having a conversation with a few other members about LC's and the nutritional content of them the other week ago. Don't remember which thread it was in. What I do remember is user "PastyWhyte" confessing that he uses is grain soak water to make LC's out of. I had recently had the same idea, but had never done it before so, I decided to try it yesterday while doing a grain run.

Even placed a few grains of rye in there.

Truth be told, I replaced the grain water in those LC's with other grain water on another burner that was further along in the boil, then sterilized the media for 45 minutes at 15-18 psi. The water that replaced the water featured in the OP was darker, and had some sediment in it.
The mediums are currently sitting safely in the vacuum of the AA x25 Steroclave. Have a clean culture on agar in a petri dish that's waiting to inoculate these things when I get into the lab next. More to come.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#20970265 - 12/13/14 08:31 AM (10 years, 1 month ago) |
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TheChief
Cube Collector



Registered: 07/09/14
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#20970269 - 12/13/14 08:35 AM (10 years, 1 month ago) |
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--------------------
jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."
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tripdawg420
low life with no life



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Re: Rye Soak Water Liquid Culture! [Re: TheChief]
#20970358 - 12/13/14 09:02 AM (10 years, 1 month ago) |
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: tripdawg420]
#20980659 - 12/15/14 04:48 PM (10 years, 1 month ago) |
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The mediums were inoculated yesterday with live, clean culture from a petri dish.

The wedges settled on the top of the mediums because the temperature reached above 80F in the lab. Body heat, the flowhood motor running, and lighting all create heat build up in the spawning laboratory. When doing multiple inoculations for hours the liquid mediums, containers, agar, and the petri dish cultures themselves become heated to ambient room temperature. That causes the wedges to float.

When the wedges are left to float, they very rapidly form a mat of mycelium that grows across the top of the culture. When said mat is allowed to colonize the top of the liquid medium, it suffocates the the culture below.
Solution: You have to go digging out the mat with a pair of forceps, or tweezers opening vectors for contamination before the mat can colonize the surface. Pevent the mat from forming all together by keeping the colonization temperature of your liquid broth mediums at 72-75F.

More to come.
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wildernessjunkie
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#20980710 - 12/15/14 04:58 PM (10 years, 1 month ago) |
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hamloaf
Q-dood ®©™√



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For anybody watching this thread. Here goes one of the mediums colonizing away.

Looking like it'll be ready to aspire into syringes early next week. We'll see.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#20994711 - 12/18/14 03:19 PM (10 years, 1 month ago) |
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Grain water from your soak/boil or grain water from dunking your grains?
Never got the difference.
-------------------- Gyo's Better Grows
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cronicr



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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
#20994992 - 12/18/14 04:19 PM (10 years, 1 month ago) |
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that's alot of lc, nice stuff hamloaf
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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ganjfather
uncle randy



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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
#20995036 - 12/18/14 04:29 PM (10 years, 1 month ago) |
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Quote:
taGyo said: Grain water from your soak/boil or grain water from dunking your grains?
Never got the difference.
I believe he means from the soak/boil.
It's the same idea as homebrewing beer. This should work 
You can either buy malt extract for making beer or you can buy grains and extract the malt with a mash tun.
I know with home brewing you basically pasteurize the grains in a mash tun, keep it at a certain temp for a certain amount of time. Doing that gets the most sugar out of your grains as possible. you would probably have a more nutritious LC if you extracted the malts with a small mash tun and followed a homebrewing guide for all-grain brewing.
Just a thought. Good luck.
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taGyo
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Re: Rye Soak Water Liquid Culture! [Re: ganjfather]
#20995042 - 12/18/14 04:31 PM (10 years, 1 month ago) |
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Thanks Ganj,
,
So it's the boil water + some nutes or just the straight boil water? Sorry, I'm not new to LCs but GLCs for sure.
-------------------- Gyo's Better Grows
TNF Q&A
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Dominus fortunae meae sum
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cronicr



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Re: Rye Soak Water Liquid Culture! [Re: taGyo] 1
#20995074 - 12/18/14 04:37 PM (10 years, 1 month ago) |
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ganj back around too...nice to see you guys around here
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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ganjfather
uncle randy



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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
#20995079 - 12/18/14 04:38 PM (10 years, 1 month ago) |
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I'm just going on a whim here and guessing, but I would think it should be just straight boil water, although I'd assume you can add nutes. You'd probably benefit from crushing whatever grains you are using, as well.
It's essentially the same idea as extracting malt from grains with a mash-tun Mashing converts the starches in the grain into sugar that the yeast eats and subsequently creates alcohol as a by product. I'm surprised more people who use LC's don't do this.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: Rye Soak Water Liquid Culture! [Re: ganjfather]
#20995090 - 12/18/14 04:40 PM (10 years, 1 month ago) |
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So am I,
That's why I'm so taken aback by this idea. I can prep grains and LCs at the same time.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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ganjfather
uncle randy



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Re: Rye Soak Water Liquid Culture! [Re: cronicr]
#20995092 - 12/18/14 04:40 PM (10 years, 1 month ago) |
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Quote:
cronicr said: ganj back around too...nice to see you guys around here
Good to see you too, my dude.
Congrats on those oh so nice moderator and trusted cultivator tags 
You deserve it!
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hamloaf
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Re: Rye Soak Water Liquid Culture! [Re: ganjfather]
#21007918 - 12/21/14 02:47 PM (10 years, 28 days ago) |
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Here goes the one of the mediums colonizing away as of this morning! Progress is visual.

There are 3 of these. All pretty much at the same point of colonization.
Have chosen to transfer this liquid mycelium straight to grains. The grain mediums are sterilized and loaded into the lab for inoculation of these LC's. Decided to go with 1 half gallon jar, and 1 quart jar of sterilized, high quality Red Milo as grainmasters for each LC. All grain mediums were soaked, boiled, dried, loaded into vessels, and sterilized for an hour and a half at 18 psi (AA 941).
The LC's will be aspired using recycled, sterilized syringes sterilized like this (click link below). http://www.shroomery.org/forums/showflat.php/Number/12098357#12098357 2 syringes per each LC will inoculate it's own separate grain medium, and be labeled accordingly (LC1, sample1, date, ect). LC's are scheduled to be aspired, and put to grains Tuesday.

For the purposes of increased GE into denser substrates, a few modifications to the way lids are usually designed and built have been made to the lids intended for use on the half gallon jars.
First, the hole size was increased from 3, 5/64th inch holes to 3, 1/8th inch holes in the same triangular cluster. Next, the filter disk size was increased from nickle-sized to quarter-sized disks.






The disk size increase allots for ample room for filtering and adhesive material to be applied to the lid the increased size in holes and cluster formation without issue. The mediums were sterilized at a higher psi for the same intents and purposes. More on the various sterilization times and why at the link below. http://www.shroomery.org/forums/showflat.php/Number/20813166#20813166
Thanks to anybody tuning into this thread. More to come as developments are made.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Re: Rye Soak Water Liquid Culture! [Re: cronicr]
#21013788 - 12/22/14 07:15 PM (10 years, 27 days ago) |
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Don't know how I missed this one That is rich for sure, did you dilute? I only didn't dilute once. It worked fine tho. Yours looks good too, can't wait to see how it turns out 
Quote:
cronicr said: that's alot of lc
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21013833 - 12/22/14 07:24 PM (10 years, 27 days ago) |
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I always wonder how an LC preforms when left alone compare to sitting on a stir plate as Statmets talks about.
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hamloaf
Q-dood ®©™√



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Re: Rye Soak Water Liquid Culture! [Re: Pastywhyte]
#21015662 - 12/23/14 06:56 AM (10 years, 27 days ago) |
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Quote:
Pastywhyte said: Don't know how I missed this one That is rich for sure, did you dilute? I only didn't dilute once. It worked fine tho. Yours looks good too, can't wait to see how it turns out 
Quote:
cronicr said: that's alot of lc

Crap! Forgot to dilute. Damnit! Think it's going to be ok though. Overly nutritious mediums are personally preferred, and, they look good, so...
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21015785 - 12/23/14 08:04 AM (10 years, 27 days ago) |
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"Overly nutritious" isn't a problem with starch nutrition from grains like it is with sweets. That's why we have absolutely no trouble colonizing on grains such as brown rice but too-sweet agar has problems. Nothing to fear. Grainwater can pack a huge nutrition punch without detriment. The way it works is that mycelium colonizes the material because nutrition is there, but it takes the enzymes to convert the more complex starch into the "sugars" it uses for energy. I believe a grainwater LC would be more ideal for the reasons that we say it's good to not let cultures consume sugars too long, in that they over time can lose their edge for more complex nutrition so-to-speak.
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