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OfflineTweexican
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Mycobag Query
    #2097019 - 11/12/03 02:08 PM (18 years, 2 months ago)

This is my pet rabbit's cat's first attempt at growing shrooms. A week ago, his bag was innoculated with B+ spores from a syringe. Both were purchased from sporebank.com. They have been incubated at around 80-90 degrees since innoculation. So far, no results.

My question is, however, does it make any difference how closely the grains inside the bag are compacted? The contents inside seem to be held together loosely.

There IS moisture in the bag as well as bits of what look and feel like metallic covered pieces of styrofoam, no idea what these are. Anyways, when I feel the growing substrate through the bag, it is sponge-like. Can anyone give me some insite? Thanks.


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Offlineangryjslice
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Re: Mycobag Query [Re: Tweexican]
    #2097041 - 11/12/03 02:12 PM (18 years, 2 months ago)

break up all the shit in the bag. it'll help speed up the process.
90 degrees is probly too hot.

~JSlice~


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OfflineBoobylip
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Re: Mycobag Query [Re: Tweexican]
    #2097046 - 11/12/03 02:14 PM (18 years, 2 months ago)

My friend has had some experience with mycobags.
1) Myco bags take a while to colonize, my friends took almost 4 weeks so don't worry if you don't see any growth for another week.
2) Once you do see growth shake up the contents of the bag, this will allow for faster colonization.
3) Moisture in the bag is fine, its a good thing.
4) Keep the bag in the dark, and try to get the temperature as close to 86'F as possible. Thats the optimal growing temp.
5) Patience is the most important thing in growing shoorms. So have some patience. Give it a week and you should see the first signs of growth.


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OfflineRoseM
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Re: Mycobag Query [Re: Tweexican]
    #2097056 - 11/12/03 02:16 PM (18 years, 2 months ago)

It can take several weeks to see results in a bag. I've seen trouble with the sporebank b+ strain recently. I've heard complaints about their recent b+ batches... and my friend, Don Quixote, watched one b+ bag take 2 months to colonize and 2 weeks to go from pin to shroom.

I would take the bags out of the incubator (they will retain more moisture... if you need to reinoculate) and watch them for another couple of weeks. If you see no growth after two more weeks, reinoculate.


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Fiddlesticks.



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OfflineBoobylip
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Re: Mycobag Query [Re: Rose]
    #2097073 - 11/12/03 02:19 PM (18 years, 2 months ago)

Quote:

Cervantes said:
I would take the bags out of the incubator (they will retain more moisture... if you need to reinoculate) and watch them for another couple of weeks. If you see no growth after two more weeks, reinoculate.




The mycobags come with all the moisture you will need from colonization to fruiting. Just try to keep your temp as close to 86'F as possible. You will see growth in a week.


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OfflineRoseM
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Re: Mycobag Query [Re: Boobylip]
    #2097106 - 11/12/03 02:27 PM (18 years, 2 months ago)

Booby, normally you would be right, but if the bag has stalled and the bags are incubating at 80-90 degrees, they WILL lose misture over the course of a month. If he hasn't seen any growth in two weeks, I'd be a little concerned. Also, it sounds like he only has one bag. I'd hate to see him need to buy another.

Tweex, did your friend inject the middle of the substrate or did your friend inject near the walls of the bag? If the middle was injected, you may have a dud. Spores like to start on the plastic itself. Otherwise, the spores may not germinate. If your incubator EVER got over 100 degrees, you may have killed your spores. Check your incubator, slow colonization can be a sign of a hot incubator.

Never let it get over 85 degrees. Too cold, and your myc will grow slowly. Too hot and it WILL die.

The best thing you can do, is get some more bags from www.mycobag.com abd a new strain from www.ralphstersspores.com

These guys are MUCH cheaper than Sporebank. Also, they seem to be more ethical (look how sporebank is trying to steal Ralphster's site www.ralphsterspores.com). I'm done dealing with Sporebank.


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Fiddlesticks.



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OfflineTweexican
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Re: Mycobag Query [Re: Boobylip]
    #2097108 - 11/12/03 02:27 PM (18 years, 2 months ago)

By the way, I forgot to mention there is a slight odor. It smells "grainy", almost like a wet dog. There doesn't seem to be any contam, I just thought I'd note this. Also, to answer your question, I followed the directions and innoculated NEAR the sides of the bags as illustrated at sporebank. However, I also shook up the contents after reading the FAQ. Does this put me in a bad spot?

Anyways, thanks for the input, Cervantes, what other kinds of trouble have you seen from sporebank? So far, all I've read is bad rap from these guys. Anyone with good results from these guys?

Also, can anyone tell me if they know anything about the substrate in the grow bags supplied by sporebank? When I inspected the bag one day, I found a sunflower seed....funny. Anyways, thanks for the input, I'll keep all updated. You guys rule.


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Edited by Tweexican (11/12/03 02:35 PM)


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OfflineRoseM
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Re: Mycobag Query [Re: Tweexican]
    #2097121 - 11/12/03 02:33 PM (18 years, 2 months ago)

My friend is growing sporebank b+ in a sporebank bag. It is the slowest growth he's ever wittnessed. And, it looks like he's getting a ton of aborts in his first bag. Lemme post some pics of his bag.


First shot


over a week later


first pins


Over a week later, same flush still growing. Cake was moved back into the incubator to speed growth.

I've also, heard of others having trouble with Sporeworks (sporebank's supplier) B+ spores. Mutants, slow growth... etc. Let me find a link.

http://www.shroomery.org/forums/showflat...rue#Post1877529
This isn't exactly the link I was looking for. There is a thread that deals with Sporebank's b+.


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Fiddlesticks.



Edited by Rose (11/12/03 03:01 PM)


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OfflineBoobylip
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Re: Mycobag Query [Re: Rose]
    #2097134 - 11/12/03 02:36 PM (18 years, 2 months ago)

My friend innoculated all his bags in the middle. I think thats why it takes so long for the bags to colonize. However they always do. I'm telling you it took two weeks for my friends bag from Sporebank to show signs of growth. After that another 1 1/2 week to colonize completely. Don't worry just yet. The less you play with it the better it will be.

Another thing it might be is that the middle of the bag already has growth you just can't see it yet.


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OfflineBoobylip
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Re: Mycobag Query [Re: Boobylip]
    #2097139 - 11/12/03 02:37 PM (18 years, 2 months ago)

Cervantes hehe next time try casing.
Fruiting in the bag is a waste. My friend cased his with coir/peat/verm/oyster shells. 300grams of first flush.



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OfflineRoseM
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Re: Mycobag Query [Re: Boobylip]
    #2097219 - 11/12/03 02:54 PM (18 years, 2 months ago)

Booby, have you tried the new (3 months old or newer) Sporebank b+ syringes? I am not talking about mycobag cultivation, I am talking about a potentially bad batch of spores from a vendor.

Yes, normally you would be right, but I have wittnessed a lot of duds with this sporebank strain. And they took a LOT longer than 1 and a half weeks to colonize completely.

Hell, the bag has taken a two weeks to grow its pins... and they're STILL not mature (13 days after pinning began and counting... no aborts yet). Have you ever heard of cubensis shrooms taking this long to form? I appreciate the input Booby but, I know someone who is had EXACTLY the same problems as Tweex... same strain, same vendor, same problem... and my friend only got growth in half his bags!

As for casing, and fruiting in bags being a waste, my friend doesn't need THAT many shrooms (he will be dunking this cake and fruiting as a cake... but that is more to try and get the cake to behave). Some of us just do this for the love of the hobby... not for MASSIVE flushes. Besides, would YOU want to case this SLOW brick of mycelia? It is growing at least three times slower than it should.


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Fiddlesticks.



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OfflineRoseM
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Re: Mycobag Query [Re: Rose]
    #2097246 - 11/12/03 02:59 PM (18 years, 2 months ago)

*By the way, I forgot to mention there is a slight odor. It smells "grainy", almost like a wet dog.

That sounds like uncolonized mycobag smell (good). If you have mixed your substrate more than once, and you still see no growth, you won't hurt yourself to reinoculate... this time, mix the bag after inoculation and, don't mix the bag again, till you see growth.

As for the substrate used, it is a mystery, you can see the verm, and grain, but the exact ingredients are a secret. They grow good, potent stuff.


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Fiddlesticks.



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OfflineTweexican
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Re: Mycobag Query [Re: Rose]
    #2107543 - 11/14/03 09:34 PM (18 years, 2 months ago)

It's been 2 weeks... still nothing. Not one sign of colonization. I'm guessing sporebank sent me a shitty syringe. I asked for a new one and they said its on its way.

Anyone else order from sporebank lately? If so I'm curious as to how its coming along?


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OfflineRoseM
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Re: Mycobag Query [Re: Tweexican]
    #2108207 - 11/15/03 02:47 AM (18 years, 2 months ago)

Well done... At least your bags didn't contaminate.


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Fiddlesticks.



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OfflineTweexican
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Re: Mycobag Query [Re: Rose]
    #2116034 - 11/17/03 01:03 PM (18 years, 2 months ago)

Finally after almost 3 weeks, this rabbit's cat's bag is showing some colonization. This took forever... just BARELY showing signs.


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OfflineRoseM
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Re: Mycobag Query [Re: Tweexican]
    #2116056 - 11/17/03 01:07 PM (18 years, 2 months ago)

Congradulastions... you're doiing fine.. and it sounds like Sporebank treated you well.


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Fiddlesticks.



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OfflineTweexican
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Re: Mycobag Query [Re: Rose]
    #2116085 - 11/17/03 01:17 PM (18 years, 2 months ago)

Yeah, not too shabby guys. Wouldn't have been able to get through it w/o your help. Kudos.

Btw, I have one more quick question, if a syringe wasn't stored in a refrigerator, would it still be viable lets say.... 3 weeks at room temp? I was also wondering, what if the spores in the syringe look a dark color? Is that good or bad?


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InvisibleGonzoCool

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Re: Mycobag Query [Re: Rose]
    #2116098 - 11/17/03 01:21 PM (18 years, 2 months ago)

when it does start to colonize in patches, crumble up the mycelium and mix it around evenly amongst the uncolonized substrate, make sure the substrate is loose and airy, crumble the mycelium up and mix it around.
the temp in the bag is normally about 3 or 4 degrees higher than the temp in the room. Good Luck guy


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OfflineRoseM
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Re: Mycobag Query [Re: GonzoCool]
    #2116112 - 11/17/03 01:25 PM (18 years, 2 months ago)

I think the syringe should be fine... these last three weeks havent been the hottest of the year.


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Fiddlesticks.



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Anonymous

Re: Mycobag Query [Re: Rose]
    #2116169 - 11/17/03 01:36 PM (18 years, 2 months ago)

i think b+ are just slow,a friend got them from earthtongue and they took over a month to colonize on brf/verm.next time he will do it on wbs and see if there is a differnce.


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OfflineTweexican
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Re: Mycobag Query [Re: ]
    #2116191 - 11/17/03 01:40 PM (18 years, 2 months ago)

Dirty man sex...

I think I just found some contam after I mixed the bag around. There seem to be tiny green patches on some of the grains inside the bag. Egh... Oh well, if at first you don't succeed...

I'm up for anything right now, don't exactly know which tek to try. I really can do any of them. Any suggestions?


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Edited by Tweexican (11/17/03 01:44 PM)


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OfflineRoseM
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Re: Mycobag Query [Re: Tweexican]
    #2116207 - 11/17/03 01:43 PM (18 years, 2 months ago)

Be patient... b+ ARE slow. EQ's are much faster. Don't toss the bag untl the green wins the battle... you never know, the white may win.


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Fiddlesticks.



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InvisibleGonzoCool

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Re: Mycobag Query [Re: Rose]
    #2116212 - 11/17/03 01:44 PM (18 years, 2 months ago)

if its a yellowish green kinda liquid its not a contam its mycelium piss, not a contam, lets hope eh


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Anonymous

Re: Mycobag Query [Re: Tweexican]
    #2116214 - 11/17/03 01:44 PM (18 years, 2 months ago)

thats sux man
but don't give up, it could be some bruising from the mixup,you'll know soon enough,can you smell from those bags or are they sealed?


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OfflineTweexican
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Re: Mycobag Query [Re: ]
    #2116228 - 11/17/03 01:47 PM (18 years, 2 months ago)

uh... they're sealed, unless sporebank sent me a bad bag...

you're right though, it won't hurt to at least hold onto it. And the smell? It smells the same, grainy, like a wet dog. I'll keep my fingers crossed.


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OfflineRoseM
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Re: Mycobag Query [Re: Tweexican]
    #2116245 - 11/17/03 01:50 PM (18 years, 2 months ago)

They ARE sealed, but if you squeeze the air filled bag lightly and sniff the patch... you can smell any contams. They don't smell like wet dog dirt... that is mycobag smell.


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Fiddlesticks.



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Anonymous

Re: Mycobag Query [Re: Tweexican]
    #2116246 - 11/17/03 01:50 PM (18 years, 2 months ago)

ive never seen or used those ready made bags,i just wonder how they get air-exchange if they're sealed?


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OfflineRoseM
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Re: Mycobag Query [Re: ]
    #2116256 - 11/17/03 01:52 PM (18 years, 2 months ago)

They don't get much... but they don't need much. You can always birth them as cakes.


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Fiddlesticks.



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Anonymous

Re: Mycobag Query [Re: Rose]
    #2116295 - 11/17/03 01:58 PM (18 years, 2 months ago)

looking at your shrooms in that pic that are over a week old(in the bag)id say thats why they are so slow,they usually shoot up real quick(less than a week) once they have formed.


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OfflineRoseM
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Re: Mycobag Query [Re: ]
    #2116305 - 11/17/03 02:01 PM (18 years, 2 months ago)

Yeah, I have no clue... all I know is b+ grew about 3x slower than the TEKs say cubes grow. Other strains haven't suffered the same fate in bags.


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Fiddlesticks.



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Offlinebeanchild
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Re: Mycobag Query [Re: Rose]
    #2116466 - 11/17/03 02:40 PM (18 years, 2 months ago)

My friend too also has got a bag from sporebank, and innoculated it with a puerto rican strain spore syringe about a week ago!! No signs of anything yet except for tinywater droplets in the bag {bottom area}, my friend is keeping the bag in a dresser drawer and the room is like 75 ish, what could he do to speed this up??


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OfflineRoseM
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Re: Mycobag Query [Re: beanchild]
    #2116480 - 11/17/03 02:43 PM (18 years, 2 months ago)

Raise the temperature 5-10 degrees... that could speed growth. Did you inject the middle of the bag, or the part between the substrate and the walls of the bag?

One week isn't long... Tweex waited 3. PR's are known to be fast colonizers though.


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Fiddlesticks.



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InvisibleGonzoCool

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Re: Mycobag Query [Re: Rose]
    #2116517 - 11/17/03 02:49 PM (18 years, 2 months ago)

with mycobags you need to inoculate at two seperate points towards the walls of the bag, not the center, and keep the temp at around 80-85 if possible, mycobags will test your patience. Good Luck


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Offlinebeanchild
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Re: Mycobag Query [Re: Rose]
    #2116522 - 11/17/03 02:50 PM (18 years, 2 months ago)

he injected the bag in small spurts in different areas of the bag i guess more in the substrae as opposed to the total edge because he was nervous about puncturing a hole in the bag,, should he put a heating blanket in the draw to raise temp??


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OfflineRoseM
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Re: Mycobag Query [Re: beanchild]
    #2116550 - 11/17/03 02:54 PM (18 years, 2 months ago)

Only if he can be sure about his temp.

Too cool is always better than too hot.

Wait more. Make sure your bags are unfolded, so the filter patch can do its job.


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Fiddlesticks.



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Offlinebeanchild
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Re: Mycobag Query [Re: Rose]
    #2116582 - 11/17/03 03:00 PM (18 years, 2 months ago)

He didn't use the whole syringe and still has a 1/3 of a syringe left, should he save this and use later if there is no signs of growth? or should he go and try to innoculate a few jars on his first pf tek attempt? By the way puerto's any good??


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OfflineRoseM
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Re: Mycobag Query [Re: beanchild]
    #2116896 - 11/17/03 04:29 PM (18 years, 2 months ago)

Wait for a couple more weeks. It'd be a shame to use the spores you didn't need.

Did you smash your bags after inoculation to mush the substrate?

If not, go ahead. You may see growth.

Otherwise... let 'em sit. If you don't have growth in three weeks, re-inoculate.


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Fiddlesticks.



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OfflineDeeBo
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Re: Mycobag Query [Re: ]
    #2116970 - 11/17/03 04:45 PM (18 years, 2 months ago)

2+ months for full colonization 1/2 pint BRF cakes with B+ syringe from sporeworks.com. Skinny mushies too  :frown: .


--------------------

Lost before found and now the world is upside down....Looked for the Earth and only found the MOON...Keep telling myself the trip will be over soon


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Re: Mycobag Query [Re: DeeBo]
    #2117059 - 11/17/03 05:07 PM (18 years, 2 months ago)

PR should be faster but, a bag is much bigger than a half pint jar.


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Fiddlesticks.



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Offlinebeanchild
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Re: Mycobag Query [Re: Rose]
    #2117402 - 11/17/03 06:32 PM (18 years, 2 months ago)

i did mush around after innoculation, what do the yeilds average on these bags?? {dry weight}


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* damn sporebank and university scientifc selfinfliction 1,431 8 02/23/05 07:52 PM
by selfinfliction

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