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OfflinetaGyo
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Open Air G2G/Flame Sterilization versus Alcohol Sanitization * 2
    #20969519 - 12/13/14 12:00 AM (10 years, 1 month ago)

I've been seeing a lot of threads and people talking about open-air g2g.

These are the problems you could encounter without knowing. I ran an open-air experiment by running an agar plate through the air.



The other one I breathed on. The breathing one got a shit ton of bacteria but nothing special. The open-air one, however, got Asperigilus.



I also alcohol sanitized my hands and touched an agar plate with my index finger:



Unidentified as of now.

Moral Of The Story:

Proper Sterile Technique Is Key!

Open-Air G2G is a bad idea, I live in a clean house and still got this. Alcohol does not replace flame sterilization.


Hopefully this thread will help someone from doing something stupid.


--------------------
Gyo's Better Grows
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Edited by taGyo (12/13/14 11:17 AM)

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Invisiblewowimflabbergasted
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20969553 - 12/13/14 12:09 AM (10 years, 1 month ago)

QFT brother  :mjk:


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InvisibleMikeBearPig
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20969588 - 12/13/14 12:18 AM (10 years, 1 month ago)

:threadmonitor:

Do you have indoor plants?

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: MikeBearPig]
    #20969591 - 12/13/14 12:19 AM (10 years, 1 month ago)

Shrooms, yup.

Nothing else though.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20970544 - 12/13/14 10:04 AM (10 years, 1 month ago)

gonna need to make a puppet to give tagyo 5 more shrooms

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: Munchauzen]
    #20970669 - 12/13/14 10:40 AM (10 years, 1 month ago)

:sunspots:,

Can't wait for my KSSS flush to compare to yours munch!


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InvisibleMunchauzen
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20970700 - 12/13/14 10:48 AM (10 years, 1 month ago)

your tub is ksss, right? its gonna destroy my cakes, lol.

but I do have some rye jars of ksss and im about to clone a pin in a few hours

Edited by Munchauzen (12/13/14 10:49 AM)

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: Munchauzen]
    #20970706 - 12/13/14 10:50 AM (10 years, 1 month ago)

Yeah but I made a lot of mistakes with it :lol:,

I'm seeing a lot of pins though so I guess I didn't make that big of a mistake. Still looking forward to your flush :super:


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InvisibleMunchauzen
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20970731 - 12/13/14 10:54 AM (10 years, 1 month ago)

well its all MS, so no real canopies. harvesting like 1-3 cakes a day. I had to stop posting on the grow thread because I ran out of space and the store is down for me to get supporter status for unlimited storage. I posted the good stuff from the first few cakes.

Edited by Munchauzen (12/13/14 10:54 AM)

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: Munchauzen]
    #20970735 - 12/13/14 10:55 AM (10 years, 1 month ago)

Why does they not want our money?


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20970749 - 12/13/14 10:59 AM (10 years, 1 month ago)

Maybe they need to get some better tech people or something.


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: wowimflabbergasted]
    #20971410 - 12/13/14 02:02 PM (10 years, 1 month ago)

How many tech people are needed to run this one website.
:smbfacepalm:

Just set up a paypal and have it accept orders, wtf.


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InvisibleMikeBearPig
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: Munchauzen]
    #20971561 - 12/13/14 02:35 PM (10 years, 1 month ago)

Quote:

Munchauzen said:
well its all MS, so no real canopies. harvesting like 1-3 cakes a day. I had to stop posting on the grow thread because I ran out of space and the store is down for me to get supporter status for unlimited storage. I posted the good stuff from the first few cakes.




There are quite a few of us who get full canopies from MS! 

Edited by MikeBearPig (12/13/14 02:36 PM)

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OfflineTheMustardTiger
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20971578 - 12/13/14 02:38 PM (10 years, 1 month ago)

Quote:

taGyo said:
How many tech people are needed to run this one website.
:smbfacepalm:

Just set up a paypal and have it accept orders, wtf.




I put a support ticket in asking about the store and someone replied with ' Sorry its complicated matter'


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: TheMustardTiger]
    #20971595 - 12/13/14 02:43 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
Quote:

taGyo said:
How many tech people are needed to run this one website.
:smbfacepalm:

Just set up a paypal and have it accept orders, wtf.




I put a support ticket in asking about the store and someone replied with ' Sorry its complicated matter'



:lmafo:,

"We can't decide who gets all the money."

Maybe they're holding it off so the day they re-open the store they'll get a massive wave of 5 dollar donations. What's that strategy called?


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OfflineTheMustardTiger
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20971607 - 12/13/14 02:49 PM (10 years, 1 month ago)

Quote:

taGyo said:
Quote:

MustardTiger66 said:
Quote:

taGyo said:
How many tech people are needed to run this one website.
:smbfacepalm:

Just set up a paypal and have it accept orders, wtf.




I put a support ticket in asking about the store and someone replied with ' Sorry its complicated matter'



:lmafo:,

"We can't decide who gets all the money."

Maybe they're holding it off so the day they re-open the store they'll get a massive wave of 5 dollar donations. What's that strategy called?




I think South Park Dubbed it the 'You can't come' technique.


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InvisibleMunchauzen
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: MikeBearPig]
    #20971610 - 12/13/14 02:52 PM (10 years, 1 month ago)

Quote:

MikeBearPig said:
Quote:

Munchauzen said:
well its all MS, so no real canopies. harvesting like 1-3 cakes a day. I had to stop posting on the grow thread because I ran out of space and the store is down for me to get supporter status for unlimited storage. I posted the good stuff from the first few cakes.




There are quite a few of us who get full canopies from MS! 




guess I didn't win the lottery

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: TheMustardTiger]
    #20971615 - 12/13/14 02:56 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:


I think South Park Dubbed it the 'You can't come' technique.



I was thinking of Cartman's amusement park :rofl2:


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20994648 - 12/18/14 03:07 PM (10 years, 1 month ago)

Still hearing about Open-Air G2G so bump.


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Offlinemushpunx
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20994834 - 12/18/14 03:47 PM (10 years, 1 month ago)

Hah dude my girlfriend was picking on me for washing up and putting on mask/sleeves/scrubs etc

I opened a pitre dish and asked her to breath on one. Then opened another for 10 seconds in open air after ousting


One had the same green/white circle and the other turned pink and banana yellow


Hehehe.  An SAB is so cheap and easy.


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: mushpunx]
    #20994855 - 12/18/14 03:51 PM (10 years, 1 month ago)

Bro,

That's what I'm saying :lol:. You really have to run an agar-plate through the air or do some experiments to realize that your environment is DIRTY but clean enough for YOU to live in, you know what I mean?

All this open-air stuff gives me a headache when you could buy a hole saw for 10-20 bucks or heat up a knife or a soup can for gods sake.


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InvisibleMrGiraffe

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20995136 - 12/18/14 04:51 PM (10 years, 1 month ago)

I used to open air G2G in a tiny ass closet with a wood floor.  I'd load it with my stuff, and spray the shit out of it with lysol with a towel stuffed under the door,  Then I'd shower, mask up, spray the shit out of again as I crept in.  It was like a human sized SAB.  I actually only ended up with one contam out of the times I did it, and that was from a bad syringe.  The original grain jars nocced with it all contaminated but one, and that last one eventually failed after the G2G. 
Now disclaimer before people jump on my shit, I eventually built a small flow hood and stopped playing the lottery and there's no way in hell I'd tell any one to attempt open air any thing.  I fucking love my flow hood lol.


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InvisibleSpitballJedi
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20995151 - 12/18/14 04:54 PM (10 years, 1 month ago)

Excellent post taGyo. 5 shrooms for you


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #20995247 - 12/18/14 05:17 PM (10 years, 1 month ago)

Quote:

SpitballJedi said:
Excellent post taGyo. 5 shrooms for you



:bow:

Means a lot SBJ. Thanks for stopping by,

:super:


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Invisibleblackdust
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20997914 - 12/19/14 07:26 AM (10 years, 1 month ago)

Quote:

taGyo said:
I've been seeing a lot of threads and people talking about open-air g2g.

These are the problems you could encounter without knowing. I ran an open-air experiment by running an agar plate through the air.



The other one I breathed on. The breathing one got a shit ton of bacteria but nothing special. The open-air one, however, got Asperigilus.



I also alcohol sanitized my hands and touched an agar plate with my index finger:



Unidentified as of now.

Moral Of The Story:

Proper Sterile Technique Is Key!

Open-Air G2G is a bad idea, I live in a clean house and still got this. Alcohol does not replace flame sterilization.


Hopefully this thread will help someone from doing something stupid.





make an agar plate and let it sit without opening. Let me know how long before it contams.

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #20997922 - 12/19/14 07:29 AM (10 years, 1 month ago)

I've actually done that,

It didn't :lol:, I used to use the same plate to cool my scalpel off because I was afraid of contams on my receiving dish. I'd literally use that dish over and over and over and over and over.

Then I realized I was being an idiot.


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Offlinetripdawg420
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20998009 - 12/19/14 08:14 AM (10 years, 1 month ago)

:thumbup:


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OfflinePsyCLown89
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tripdawg420]
    #20998586 - 12/19/14 11:43 AM (10 years, 1 month ago)

Only saw the thread now, awesome thread indeed! :laugh:


BTW noticed quite a bit of supporter talk going on - drop geokills or Asante a PM about supporter accounts.


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: PsyCLown89]
    #20998590 - 12/19/14 11:44 AM (10 years, 1 month ago)

:eek:,

I will right now.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20998608 - 12/19/14 11:50 AM (10 years, 1 month ago)

What's a supporter account?


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: mushpunx]
    #20998623 - 12/19/14 11:55 AM (10 years, 1 month ago)

unlimited storage, color gradient name, larger avatar, faster colonization

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Munchauzen]
    #20998633 - 12/19/14 11:57 AM (10 years, 1 month ago)

Quote:

Munchauzen said:
faster colonization



:solidnod:

:canthelpbutlaugh:


--------------------
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20999249 - 12/19/14 02:58 PM (10 years, 1 month ago)

:dawerp:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Munchauzen]
    #20999270 - 12/19/14 03:04 PM (10 years, 1 month ago)

Quote:

Munchauzen said:
unlimited storage, color gradient name, larger avatar, faster colonization





Ahahahahaha


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Munchauzen]
    #21208285 - 01/31/15 06:57 PM (9 years, 11 months ago)

Quote:

Munchauzen said:
unlimited storage, color gradient name, larger avatar, faster colonization




:havesomescience:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21250514 - 02/09/15 07:59 PM (9 years, 11 months ago)

Quote:

taGyo said:
Hopefully this thread will help someone from doing something stupid.





:lol:

If you can just save one....


Nice post dude.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21250523 - 02/09/15 08:00 PM (9 years, 11 months ago)

This was my last open-air experiment:


Thanks SBJ,
Figured I should put it in my sig :lol:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21250721 - 02/09/15 08:34 PM (9 years, 11 months ago)

Looks like pasta wheels and cream sauce. :lol:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21250877 - 02/09/15 09:08 PM (9 years, 11 months ago)

Can I give you 5 shrooms for the worst hand-writing I've ever seen?
:tongue2:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Fuzz-nutter]
    #21250988 - 02/09/15 09:37 PM (9 years, 11 months ago)

Quote:


Bro,

That's what I'm saying :lol:. You really have to run an agar-plate through the air or do some experiments to realize that your environment is DIRTY but clean enough for YOU to live in, you know what I mean?

All this open-air stuff gives me a headache when you could buy a hole saw for 10-20 bucks or heat up a knife or a soup can for gods sake.




Of course. The main reason the environment is so dirty in the first place is because you're in it. It's kind of funny the trouble some people have gone through to try to create a sterile room only to put the filthiest thing in the house inside it, a human being :lol:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Kizzle]
    #21251075 - 02/09/15 09:56 PM (9 years, 11 months ago)

I am going to get some dishes just so I can do some stuff with my kids.  That stuff is always so interesting to do. 

I will prob end up with pasty plates though.

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21255790 - 02/10/15 09:24 PM (9 years, 11 months ago)

lol Gyo we both use blue agar :awesome:

I do pretty much all of my oyster work in open air, but I haven't tried a g2g. I may. Oysters are fucking FAST!


--------------------

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
    #21298116 - 02/19/15 10:34 AM (9 years, 10 months ago)

is open air and still air rooms the same?

:lolsy:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
    #21298896 - 02/19/15 01:07 PM (9 years, 10 months ago)

Quote:

NumeroEno said:
lol Gyo we both use blue agar :awesome:

I do pretty much all of my oyster work in open air, but I haven't tried a g2g. I may. Oysters are fucking FAST!



How do you get away with this?

@Kizzle,

:lol:, can never win man.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Fuzz-nutter]
    #21298944 - 02/19/15 01:14 PM (9 years, 10 months ago)

Quote:

Fuzz-nutter said:
Can I give you 5 shrooms for the worst hand-writing I've ever seen?
:tongue2:



:lmafo:,

My girl is writing everything from now on :lol:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21298971 - 02/19/15 01:18 PM (9 years, 10 months ago)

Quote:

taGyo said:
Quote:

NumeroEno said:
lol Gyo we both use blue agar :awesome:

I do pretty much all of my oyster work in open air, but I haven't tried a g2g. I may. Oysters are fucking FAST!



How do you get away with this?

@Kizzle,

:lol:, can never win man.





1. Speed. Faster colonization methods can be used to overcome less then sterile media or procedures.

2. The cultivator is more dangerous then the air in most cases in my opinion. Our bodies are covered in bacteria and molds. we lose 30,000 to 40,000 dead skin cells every minute. Our breath is full of bacteria.

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21298973 - 02/19/15 01:19 PM (9 years, 10 months ago)

Shit man I need to work with some oysters.

Has anyone ever crossed psychs and edibles?


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21298980 - 02/19/15 01:20 PM (9 years, 10 months ago)

:thataintright:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: concrete_666]
    #21299060 - 02/19/15 01:38 PM (9 years, 10 months ago)

Quote:

concrete_666 said:
:thataintright:




I think lucy may have gotten him :rofl:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21299066 - 02/19/15 01:39 PM (9 years, 10 months ago)

Quote:

taGyo said:
This was my last open-air experiment:






Now THAT'S gross. Cracked it trying to take tape off.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21306926 - 02/21/15 05:49 AM (9 years, 10 months ago)


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: Newt]
    #21307069 - 02/21/15 07:28 AM (9 years, 10 months ago)

:wellthatshorrifying:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #21307076 - 02/21/15 07:31 AM (9 years, 10 months ago)

Kissing agar would result in massive contamination outbreak.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: hamloaf]
    #21307087 - 02/21/15 07:37 AM (9 years, 10 months ago)

All of your grows from that point forward would fail, whether you tossed the plate or not.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #21307346 - 02/21/15 09:17 AM (9 years, 10 months ago)

Not necessarily.  Great peril would be created in the spawn lab creating the vector for the peril of future generations, that's for sure, but, to save future generations, I would just remove the contaminated culture from the lab, discarding it outdoors as quick as possible. 

A 3 day intensive cleaning regimen would then begin consisting of the removing of all cultures from the spawning area, the scrubbing down of all surfaces of the spawning lab with a 1:10 bleach:water solution would be employed 3 times a day for three days.  Stock cultures would then be returned to to generate more subcultures, and eventually, grain spawn.


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Edited by liquidmyce (02/21/15 10:02 AM)

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: hamloaf]
    #21307356 - 02/21/15 09:20 AM (9 years, 10 months ago)

Gonna have to disagree with you on this one Ham,

Pretty sure the Mobil Contamination Unit would make sure nothing grew.

Jokes Hamloaf, jokes, I've had plenty of worse plates :lol:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21312387 - 02/22/15 10:53 AM (9 years, 10 months ago)

where the hell is the SAB plate?

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21312499 - 02/22/15 11:17 AM (9 years, 10 months ago)

hopefully used to knock up some grains that are now spawned and fruiting :rockon:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21312507 - 02/22/15 11:19 AM (9 years, 10 months ago)

SAB plate?


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21312553 - 02/22/15 11:26 AM (9 years, 10 months ago)

I think he means he would like to see you do the same thing in a SAB as a comparison.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21312572 - 02/22/15 11:29 AM (9 years, 10 months ago)

Oh,

No problem I'm setting my SAB up tonight actually (If I find the motivation 'cause lord knows that's a problem). I'll wipe it down real good and shit and do two things:

Drag a plate through the air and open a plate and set it off to the side while I do my work.

If both don't contam then shout out to my SAB but I'm gonna guess the one I drag may not contam but the one I leave to the side will.

I'll keep this thread updated.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21312614 - 02/22/15 11:39 AM (9 years, 10 months ago)

If I were still inside of an SAB today, I would plastic off a small section of an area, and set up my SAB inside of a sterile lab environment, using the same procedures to enter the lab as I do with my flowhood. 

There is such a thing as Still Air Laboratories & Still Air Laboratory Techniques and Procedures.  :thumbup:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21313265 - 02/22/15 01:52 PM (9 years, 10 months ago)

Considering when knocking up plates I do not wave them around in the air but lift up the lid for a few seconds for work I find this experiment a bit rubbish. A+ for effort. I look forward to your future projects. :cheers:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21313281 - 02/22/15 01:56 PM (9 years, 10 months ago)

:popcorn:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21314337 - 02/22/15 06:34 PM (9 years, 10 months ago)

Quote:

blackdust said:
Considering when knocking up plates I do not wave them around in the air but lift up the lid for a few seconds for work I find this experiment a bit rubbish. A+ for effort. I look forward to your future projects. :cheers:




I once had a poured plate pop up with a single spot of bacteria, since it was wasted I decided to take it into my basement greenhouse and wave that bitch around just to see what kind of stuff I'd pick up.  That plate sat around for like 5 days with only the first spot of bacteria still on it.  I opened it up again in my upstairs kitchen/living room and got mold within 3 days.  I know there's shit growing in my basement.  It would grow on the extension cords and stuff when I was running the coolmist 24/7.  Best I can figure is the coolmist running 24/7 keeping the air at 99% and the walls of the greenhouse saturated maybe collected the spores and kept them from floating around to much :shrug:.  Not the most scientific thing, but I was still surprised the plate didn't get covered until I opened it upstairs.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: MrGiraffe]
    #21314366 - 02/22/15 06:39 PM (9 years, 10 months ago)

I recconmend any doing work in a still air room or a SAB to spray either

A. water around the work area to catch the molds to be wiped away

or

B. disinfectant spray


I always used disinfectant spray


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21314623 - 02/22/15 07:35 PM (9 years, 10 months ago)

I spray my SAB with a mix of water, dish soap, rinse aid, and bleach. I was using lysol before, and now I get much fewer contams. The rinse aid just helps me see better. Lysol wipes are the shit though.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316145 - 02/23/15 06:27 AM (9 years, 10 months ago)

Quote:

NumeroEno said:
I spray my SAB with a mix of water, dish soap, rinse aid, and bleach. I was using lysol before, and now I get much fewer contams. The rinse aid just helps me see better. Lysol wipes are the shit though.



:sunspots:

Iso and water for me. Paper towel to white it down.

Quote:

blackdust said:
Considering when knocking up plates I do not wave them around in the air but lift up the lid for a few seconds for work I find this experiment a bit rubbish. A+ for effort. I look forward to your future projects. :cheers:




It's an experiment to show you you're not in control of everything around you. To be honest I did a very quick open-air agar syringe drop and I saw no contams lol.

I don't think anyone is ever saying it's impossible, but for consistent results people prefer an SAB.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21316272 - 02/23/15 07:22 AM (9 years, 10 months ago)

The reason I like the disinfectant spray is that the spray is finer and thus goes through the air better the catch more debris. I'm sure they have spray bottles that can do this to though. I wipe everything down with iso.


===
Gy0, you would be happy to know that my still air room has become overwhelmed with fruiting chambers and I was forced to pull out my SAB to do work in my bedroom. I didn't have any problems using the SAB but prefer the still air room. Had to clean it out good since I was fruiting in it. :rofl:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316277 - 02/23/15 07:24 AM (9 years, 10 months ago)

how do you keep the dirtiest thing in your house out of the SA-room and still be able to do work in it?
do you have a glory hole in your wall? :tongue2:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21316331 - 02/23/15 07:50 AM (9 years, 10 months ago)

1. shower / brush teeth
2. make sure all fans are off for an 1 hr before work
3. gloves / face mask
4. Spray room and wait 5 min
5. Wipe everything with iso / use torch lighter
6. make use of SHIPs and directly injecting bags
7. Know in advance what will be done and work fast
8. cause as little air movement as possible


**I have also always ran a air purifier in the bedroom. The still air room has always been a closet for me. Not sure if the air purifier has any effect. I seen still air rooms set up many different ways.

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316342 - 02/23/15 07:53 AM (9 years, 10 months ago)

I'm impartial to any method. Part of the whole philosopher thing, I do what works. I just know that you're doing things with prior experience which vastly outweighs noobiness.

Your methods aren't really out there so much as we just don't suggest them to new members,

:shrug:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316348 - 02/23/15 07:56 AM (9 years, 10 months ago)

ships only? so you dont do agar in the room then?
I still prefer a box so I can keep myself out of the SA environment,
no matter how many showers you take you are still the dirtiest thing in your house :tongue2:
not just you, I am as well, not meant as an insult!

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21316433 - 02/23/15 08:29 AM (9 years, 10 months ago)

I know space. I have done a few dozen agar plates in a still air room. Here is my old agar bottle. Never got to much into it as I diddnt really need it for cubes.




I did just make some pasty plates a few days ago in my SAB though. I like this new no-pour agar. Im getting my skills with agar and cubes so when I get some more exotic species I will have the confidence to do what I want. The main species I am working towards at the moment is

Psilocybe cyanofriscosa
Psilocybe Subaeruginosa
Panaeolus (Copelandia)

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21316589 - 02/23/15 09:09 AM (9 years, 10 months ago)

BD, I'll hook you up with some cyanofriscosa/allenii if I can get my print to germinate. I'll send you what's left of the print after I get it going. It is a wild print so you'll need either antibiotic agar or extreme luck to get it going. I can also send you 50mgs of gentamycin sulfate, which is enough for a liter of agar.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21317572 - 02/23/15 12:49 PM (9 years, 10 months ago)

Quote:

spacechildo said:
ships only? so you dont do agar in the room then?
I still prefer a box so I can keep myself out of the SA environment,
no matter how many showers you take you are still the dirtiest thing in your house :tongue2:
not just you, I am as well, not meant as an insult!



Your trash can is a FUCK ALL of a breeding ground for contamination.  After that, pets (if you have any).


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21317576 - 02/23/15 12:50 PM (9 years, 10 months ago)

Yeah but no one is cultivating in a trash can or around one. I think he meant dirtiest thing you SHOULD be introducing to a Still-Air environment lol.


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Edited by taGyo (02/23/15 12:51 PM)

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21317587 - 02/23/15 12:52 PM (9 years, 10 months ago)

Quote:

taGyo said:
Yeah but no one is cultivating in a trash can Ham.



:facepalm:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: cronicr]
    #21317590 - 02/23/15 12:52 PM (9 years, 10 months ago)

:laugh2:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: cronicr]
    #21317597 - 02/23/15 12:53 PM (9 years, 10 months ago)

:notamused:

Really Cron? :lmafo: Okay, try not taking out the trash for a few days, then removing the plastic liner and fruiting in that.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21317668 - 02/23/15 01:11 PM (9 years, 10 months ago)


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21317709 - 02/23/15 01:20 PM (9 years, 10 months ago)

:ugh:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tripdawg420]
    #21317729 - 02/23/15 01:25 PM (9 years, 10 months ago)

I wanna try growing oysters on pasteurized garbage.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
    #21317744 - 02/23/15 01:28 PM (9 years, 10 months ago)

:thisfuckinguy:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21317778 - 02/23/15 01:33 PM (9 years, 10 months ago)

Hey Stamets uses them to clean up oil slicks. I may do it one day for shits and giggles. I've already successfully grown them on cardboard from the dumpster at my apartment complex :lol:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
    #21317797 - 02/23/15 01:39 PM (9 years, 10 months ago)

Didn't even clean it?!?!

Yo Eno do you want PESA?

Message me if you do.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21318165 - 02/23/15 02:44 PM (9 years, 10 months ago)

Yeah I'll take some PESA. I love Amazonian cubes :awesomenod:

All I did was cut it up and pasteurize it. I got a box that wasn't covered in filth. When I said garbage I didn't mean pizza crusts and meat trimmings :lol:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
    #21318171 - 02/23/15 02:44 PM (9 years, 10 months ago)

:pm: addy.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21318175 - 02/23/15 02:45 PM (9 years, 10 months ago)

fuck ya pesa are nice :thumbup:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tripdawg420]
    #21318185 - 02/23/15 02:47 PM (9 years, 10 months ago)

I pulled over an ounce dry first flush,

About 2" tall, meaty bastards.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tripdawg420]
    #21318188 - 02/23/15 02:48 PM (9 years, 10 months ago)

I'll PM you my address after work. I'm just getting off a break.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
    #21318283 - 02/23/15 03:04 PM (9 years, 10 months ago)

:super:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21318479 - 02/23/15 03:41 PM (9 years, 10 months ago)

Quote:

taGyo said:
Yeah but no one is cultivating in a trash can or around one. I think he meant dirtiest thing you SHOULD be introducing to a Still-Air environment lol.




It' merely the fact that a trashcan is in your home with rotting garbage in it and our homes are were most of our both sterile work is completed, and where our mushrooms fruit.  Contaminates breed in there and are transferred into the lab, or cross-contaminated into your SAB, Still air environment, whatever by yourself, and, or mobile contamination units. 

Are you not familiar with Still Air Sterile Lab techniques and procedures??


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21318488 - 02/23/15 03:42 PM (9 years, 10 months ago)

Nope,

Care to send me a link? I actually just started three months ago so excuse me if I have no idea what I'm doing lol.

I wash up like RR does, put on gloves and a clean shirt before prep. I very, very rarely get contams in my SAB. 99% of the contams I get I put there.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21318520 - 02/23/15 03:49 PM (9 years, 10 months ago)

why stick your whole dirty self into a vast room when you can wash a pair of gloves and stick 'em in a SAB? :shrug:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21318530 - 02/23/15 03:51 PM (9 years, 10 months ago)

You don't need no link, man, you could search yourself and find your own link.  :cool:

If I didn't have a flowhood, I would plastic off a small area and create a makeshift poorman's sterile lab, and keep my SAB inside of the makeshift lab, following sterile lab techniques before EVER entering the lab.  The plasticed off area makesshift as a GREAT place to incubate your spawn inside of too.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21318542 - 02/23/15 03:53 PM (9 years, 10 months ago)

Nah,

I'm good. Like I said I don't plan on bringing my garbage into my SAB nor do I get contams.

:shrug:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21318575 - 02/23/15 04:01 PM (9 years, 10 months ago)

Quote:

THE_LOAF said:
You don't need no link, man, you could search yourself and find your own link.  :cool:

If I didn't have a flowhood, I would plastic off a small area and create a makeshift poorman's sterile lab, and keep my SAB inside of the makeshift lab, following sterile lab techniques before EVER entering the lab.  The plasticed off area makesshift as a GREAT place to incubate your spawn inside of too.




I know what your talking about.
Quote:

MudFuka does this

I have no pictures of this step so hears a pic of my sterile work area.:shrug:


this is my new sterile work area smaller but more effective.




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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21318661 - 02/23/15 04:23 PM (9 years, 10 months ago)

I spy an SAB.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21320684 - 02/23/15 11:59 PM (9 years, 10 months ago)

Quote:

taGyo said:
Nah,

I'm good. Like I said I don't plan on bringing my garbage into my SAB nor do I get contams.

:shrug:



Good for you.  I should hope you have more sense than to bring garbage into your SAB.  As far as contamination goes, you haven't been in this hobby long enough yet.  Give it some time.  Nobody never gets contamination.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf] * 1
    #21321215 - 02/24/15 05:25 AM (9 years, 10 months ago)

Quote:

THE_LOAF said:
Nobody never gets contamination.




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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: eatyualive]
    #21321358 - 02/24/15 06:43 AM (9 years, 10 months ago)

Quote:

eatyualive said:
Quote:

THE_LOAF said:
Nobody never gets contamination.








I spent about three months thinking I was a bad ass ....then got smacked so hard by the green monster I had to TOTALLY shut down....clean the hell out of everything ...ten times lol. That was last winter and I'm just now getting over it . Very humbling experience to say the least.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
    #21321385 - 02/24/15 06:53 AM (9 years, 10 months ago)

Indeed.  Getting bitch-slapped, and boot-worked by contamination is a kind of unwritten rite-of-passage into this hobby.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21321419 - 02/24/15 07:06 AM (9 years, 10 months ago)

Yep....I think it all started with one bad syringe....g2g ended up being about 50 jars and ten tubs all told....sucked bad! I went from hero to zero in about a week. lol.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
    #21321510 - 02/24/15 07:41 AM (9 years, 10 months ago)

Bacteria has been the worst for me. I can still remember losing at least 6 lbs of mushrooms b/c of it. Taught me to be super picky about spawn though.


:kingcrankey:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #21321571 - 02/24/15 08:04 AM (9 years, 10 months ago)

Quote:

blackdust said:
Bacteria has been the worst for me. I can still remember losing at least 6 lbs of mushrooms b/c of it. Taught me to be super picky about spawn though.


:kingcrankey:




Yep...been there done that as well...I started pc'ing my grains longer,quit trying to incubate with a heating pad/towel contraption I had and made sure I had no water pooling at the bottom of my jars (  let my grain dry out overnight) poof no more bacteria.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
    #21321592 - 02/24/15 08:11 AM (9 years, 10 months ago)

Force-incubation in this hobby is a no good.  Mycelium best incubates at the same temperature as humans, and is why "room-temperature" 70-75F) is prescribed to incubate mushroom mycelium @.  Room temperature is just a grossly simplified way of saying incubate mushroom mycelium @ 70-75F.  The same temperature range we incubate ourselves at.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
    #21321637 - 02/24/15 08:24 AM (9 years, 10 months ago)

Grain prep can be tricky. I have the most experience with rye but just started using WBS and popcorn. Had some really good popcorn made the other day with the grain haul intact the after the PC cycle the fuckers exploded on me a bit. Must of hydrated them to much before the cycle to cause the hauls to burst. Remember, grain hauls are contam resistant and thats one of the reasons that burst grains are bad.

:awetrippie:


Ham, Some people have colonization and fruiting rooms in basements, by windows, or live in an older house that is not insulated well. The mean temperature of my grow room right now is 65F. 65F is not a good temperature for Psilocybe cubensis. The cold tempeature slows down colonization and the culture is not as vigorous and is more prone to stalling out, weak fruits, drying out, etc. Even though my fruiting room has a mean temp of 65F the majority of the house is at 73F. The grow room is closed off from the air vents and thus does not get warmed up like the rest of the rooms of the house. I was thinking of having a space heater by the door to keep the temps up to 70 - 75F (and it was working) but I had to move the space heater b/c my wife wanted it. I tried to run a second space heater for the room but my breaker can't handle the load and blows.

I think Kizzle was talking about passive incubation a few months ago. Suggested having spawn jars and bags to be sub,merged in vermiculite to help insulate the spawn. Spawn generates heat, so if the verm can keep the heat in them a desired temp could be reached. The time difference can be as large as 15 days in colonization times because of temp. This is just my experience.

I used to live in the Southbay of Cali and never had temp or RH issues since the weahter was the same everyday. About 70F with a nice ocean breeze as I lived within 1 mile of the ocean. Now with me living North East again and am dealing with a harsh winter while living in a trailer my growing skills are being tested a bit as this is all new to me. Rh is easy to take care of but keep the room warm enough is my challenge at the moment.

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf] * 1
    #21321852 - 02/24/15 09:22 AM (9 years, 10 months ago)

Quote:

THE_LOAF said:
Force-incubation in this hobby is a no good.  Mycelium best incubates at the same temperature as humans, and is why "room-temperature" 70-75F) is prescribed to incubate mushroom mycelium @.  Room temperature is just a grossly simplified way of saying incubate mushroom mycelium @ 70-75F.  The same temperature range we incubate ourselves at.



This forum needs to be simplified. If we started using the term incubate for room temperature and forced-incubation for actually incubating inside of something, people would be confused as hell. You would see crazy TiT incubators everywhere. You're saying the same thing as everyone else but getting all butt hurt over terminology. Sure you can be technically correct and call a house an incubator, but jesus christ.



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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
    #21321884 - 02/24/15 09:32 AM (9 years, 10 months ago)

I'm not butthurt at all.  :confused: 

and don't appreciate being falsely accused of that.  Seems to me that the ones who are so quick to accuse others of being butthurt are the ones who are butthurt.  Thank you for your concern though.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21321893 - 02/24/15 09:34 AM (9 years, 10 months ago)

Aight dude sorry then. I'll knock it off. I'm just trying to keep future noobs from roasting their jars. :cheers:


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
    #21321945 - 02/24/15 09:50 AM (9 years, 10 months ago)

posts like these are confusing to me and probably a lot of newbs too:

Quote:

THE_LOAF said:
When will you kids learn, room temperature is INCUBATION.  :facepalm:




Quote:

THE_LOAF said:
NEVER INCUBATE IN THIS HOBBY :stonedjerk:




where do you keep your jars then? if inside is incubating and incubating is bad?
I might be nit picking now but I kinda agree with flabbergasted, it gets confusing :shrug:

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: spacechildo]
    #21321957 - 02/24/15 09:51 AM (9 years, 10 months ago)

I think he was being facetious space.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
    #21322085 - 02/24/15 10:27 AM (9 years, 10 months ago)

Correct.  My spawn INCUBATES at 70-75F, and 50% RH inside of my makesshift, poorman's sterile laboratory.

   

   

   


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: wowimflabbergasted]
    #21322134 - 02/24/15 10:40 AM (9 years, 10 months ago)

context. It's all about context.


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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21322210 - 02/24/15 11:01 AM (9 years, 10 months ago)

Wow :lol:,

You guys are really having a go.

Relax guys, my APE tub just triched out and I had other stuff trich out before.

Nobodies perfect lol. I don't think I'm a badass, I just think I can acquire and use information very well. Once again, for three months into this hobby and my first pin being less then 2, I'm doing GREAT. That's all that matters to me and that's why I can say my APE tub triched out (Because I used bacterial spawn on purpose from a dirty syringe) and take pictures and put it all over the internet because I don't believe in ego in failure.

I fucked up, learn from it, that's why I have a 50 page grow log of straight fuck ups, because people have read it and corrected themselves.

At the end of the day I feel like you older guys should stop trying to get all the praise and put the attention where it deserves, on new cultivators who are bringing this into the next generation. Work on your experiments, post your results with like-minds, write up teks and simplify the hobby for EVERYONE. Is that not the goal? Even you Blackdust, who seems hell bent on taking Ham's side because you're both part of the tribe. I understand cleanliness, I just don't need someone who's been doing this for YEARS to come down on me and go "BlahBlahBlah, proper lab technique, blahblahblah." when in all reality it's just a box with holes cut in it. I spend my time on the shroomery helping people, not arguing politics or semantics.

I grow drugs bro, and help other people grow them and I do a damn good job at both. Already 90+ 5 Shroom ratings?

:cheers:


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Edited by taGyo (02/24/15 11:08 AM)

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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21322266 - 02/24/15 11:13 AM (9 years, 10 months ago)

I've been doing this seriously since about August, so 6 months. I've had every contam in the book, but bacteria has been my biggest headache. I've only lost 2 tubs to trich and one to mucor. But I agree. Everyone will have to battle contams at some point.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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Offlinetourrat
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: NumeroEno]
    #21322482 - 02/24/15 12:11 PM (9 years, 10 months ago)

This arguement about incubating is just plain stupid. HAM I get where you're coming from but god damn dude you're doing nothing but confusing the hell out of people for the sake of some stupid terminology beef. Room temp is a whole different thing than incubation when used in the context were using it in.

The reason we don't incubate in an incubator is because if you use an incubator you end up heating the bottom of the jar slightly more than the rest of the jar which causes condensation....which in turn drips to the bottom of the jar where it becomes the perfect breeding ground for bacteria which favors the extra warmth your incubator is so conveniently creating.

This doesn't happen nearly as bad when just left at "room temp" because the jat is warm evenly from all directions.

I realize 90% of us know this but for the few that don't this small tied bit of info can save a lot of headache.


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Knee deep in the hotel tub.....

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OfflinehamloafM
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tourrat]
    #21322600 - 02/24/15 12:44 PM (9 years, 10 months ago)

Sorry for your confusion, but I am going to continue to use the term incubation correctly in hopes of clearing up any confusion.  It is this cultivators opinion that the falling into line of it's meaning will piggy-back on my efforts.  Thank you for your concern though.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Offlinetourrat
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf]
    #21322663 - 02/24/15 01:01 PM (9 years, 10 months ago)

This begs the question....my house isn't heated at all right now...its simply at ambient temperature... you're claiming that's incubating?...just to clarify.

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InvisibleTomandjerry58
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: hamloaf] * 1
    #21322743 - 02/24/15 01:25 PM (9 years, 10 months ago)

I think there is a difference with open air. Back when I first started I had this little closet with a vinyl floor. I could close the door in their and it was ok.Did g2g just fine with a mask and gloves. But as far as doing g2g in a kitchen or bedroom or any part of the house that's not closed in and clean. Mucking trich, penicillin, pink orange. Ive had them all

The sab you guys recommended works great. Taking this shit to new heights with great ease. Even had the gf doin tiger drops in the sab last week and there looking great and shes about as new to this shit as new gets. Probly gonna have her try some agar transfers next.

If I had any advice to give to noobs is to build one of these things. Almost a necessity in this hobby. YOu can find the drill bit at lowes. Hook it up and run it in reverse to make the holes. Apply a small amount of pressure while doing this. Then box it back up and return it!! Get your 40 bucks back!

Watch your temps while pasteurizing. Me and a buddy of mine got into a large grow a while back. And he was doing all the pasteurization and mixing. Pretty sure the guy was just getting high and not watching the stove. Probably lost a 100 lbs of sub. And at least 20 in spawn or more. He kept asking me if 200 f was ok and I thought its never been a problem before. But fuck we lost everything.

edit- nice grow loaf:eek:

Edited by Tomandjerry58 (02/24/15 01:27 PM)

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OnlinecronicrMFacebook
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Tomandjerry58]
    #21323851 - 02/24/15 05:27 PM (9 years, 10 months ago)

ENOUGH CRAP PEOPLE! STICK TO THE TOPIC AT HAND OR SUFFER THE WRATH!


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisiblebodhisattaMDiscordReddit
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: cronicr]
    #21323862 - 02/24/15 05:29 PM (9 years, 10 months ago)

:uhoh:

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: bodhisatta]
    #21323880 - 02/24/15 05:32 PM (9 years, 10 months ago)

:lmafo:


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Offlinekushroom
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #21325058 - 02/24/15 08:35 PM (9 years, 10 months ago)

Man im hear to tell ya, even with mold in the location, with sterile techniques you can have flawless, clean results nearly everytime!


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:/



All submitted posts are by Someone Who Isn't Me (SWIM) -  and in any event are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: kushroom]
    #21328292 - 02/25/15 01:57 PM (9 years, 10 months ago)

:super:


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Gyo's Better Grows
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