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Offlineblackout
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Making the most of your apparently "empty" syringe, making a long lasting LC
    #20962227 - 12/11/14 02:16 PM (9 years, 5 months ago)

When you inject a spore syringe you usually do not get everything out. Spore solution still remains in the needle. You can easily test this if you have old empty syringes lying about, suck in some water and squirt as much out as you can, now suck back in and see that liquid is pulled back in (which was in the needle)

If you flame sterilize the needle you will kill all the spores and they will typically just vaporise out.

You could suck up sterile water to try and get it out, if doing this its probably better to just wait until the last 1-2CC and suck up water, as there will be minimal spores in the needle. This method of diluting spores to stretch them is certainly nothing new, but I cannot recall seeing people acknowledging the needle volume. People may already have syringes they recapped and discarded which could well have viable spores still in them.

I was sure I had some spore solution left and was going to make an LC. I then found my syringe was empty but thought about the needle volume, as I was doing tests on flaming needles recently and was well aware that a few drops are in a needle.

Instead of injecting into my prepared LC I poked the needle into the SHIP and drew in sterile air. When doing this I could see spore solution drawn back into the syringe body confirming it had not dried out. I only sucked in a tiny amount of LC. I believe the spores do better in a small amount as its more concentrated and there is more a greater surface area to liquid volume ratio so they should be aerated better. After sucking in some LC I drew a bit more sterile air into the syringe.

If the LC does take off I can then inject it back into the LC jar I planned to use.

My LC is very dilute, I was reading an old post by Workman how uses as low as 0.1-0.5% solution.

If you want to try long term storage of an LC you could suck up a tiny amount of very dilute LC and let it grow in the syringe. Now draw in some sterile distilled water. Let this settle for a day or so with the needle pointing upwards so the myc falls down against the plunger. Now you can expel the water over a sink or outside, you hold the syringe needle pointing upwards so the myc remains near the plunger and none is squirted out. What you have done is dilute any remaining nutrients down and squirted them out, so now you can suck up sterile water once again to further dilute. You could repeat this over and over if you really want to be sure to be rid of almost all nutrients. This could last a very long time, it could possibly last longer than the spore syringe otherwise would have.

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Invisibleblackdust
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: blackout]
    #20962270 - 12/11/14 02:27 PM (9 years, 5 months ago)

:billymaythumbup:

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OfflineKizzle
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: blackdust]
    #20963557 - 12/11/14 07:38 PM (9 years, 5 months ago)

IF you were planning to make an LC you can always make the most of your "empty" spawn jars as well. Assuming it's still sterile of course, like an empty g2g master should be.


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OfflinetaGyo
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: Kizzle]
    #20963571 - 12/11/14 07:43 PM (9 years, 5 months ago)

Quote:

Kizzle said:
IF you were planning to make an LC you can always make the most of your "empty" spawn jars as well. Assuming it's still sterile of course, like an empty g2g master should be.



That's a big ass LC.


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InvisibleMunchauzen
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: taGyo]
    #20963597 - 12/11/14 07:50 PM (9 years, 5 months ago)

Few years back, I was making gallon LCs of oysters to dump on tree stumps around town.

Edited by Munchauzen (12/11/14 07:50 PM)

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OfflinetaGyo
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: Munchauzen]
    #20963687 - 12/11/14 08:04 PM (9 years, 5 months ago)

How'd that work out for you?


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InvisibleMunchauzen
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: taGyo]
    #20963717 - 12/11/14 08:09 PM (9 years, 5 months ago)

Excellent. My tek was just to put a brick or cinderblock on the stump and pour the LC inside it. The blocks help keep the mycelium from drying out while it establishes itself. Then huge fruits would follow :omgawesome:

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Offlineblackout
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: Kizzle]
    #20964933 - 12/12/14 01:28 AM (9 years, 5 months ago)

Quote:

Kizzle said:
If you were planning to make an LC you can always make the most of your "empty" spawn jars as well.


Good idea, there is nearly always some myc clinging to the glass. People could get in the habit of properly sealing the "empty" jars in their sterile environment and just not bother washing them right away.

I forgot to mention this about the syringe, there is very often spores trapped/stuck on the plastic body and possibly on the rubber plunger. So if I was to suck up water and squirt it out I might not get use of these. I could see some trapped at the top end near the needle, where the plastic has a sharp "corner".

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OfflinetaGyo
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: Munchauzen]
    #20965962 - 12/12/14 09:46 AM (9 years, 5 months ago)

Quote:

Munchauzen said:
Excellent. My tek was just to put a brick or cinderblock on the stump and pour the LC inside it. The blocks help keep the mycelium from drying out while it establishes itself. Then huge fruits would follow :omgawesome:



Did it work?


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Invisiblewowimflabbergasted
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: blackout]
    #20966067 - 12/12/14 10:07 AM (9 years, 5 months ago)

Quote:

blackout said:
Quote:

Kizzle said:
If you were planning to make an LC you can always make the most of your "empty" spawn jars as well.


Good idea, there is nearly always some myc clinging to the glass. People could get in the habit of properly sealing the "empty" jars in their sterile environment and just not bother washing them right away.




That's what my avatar is.


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OfflinetaGyo
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: wowimflabbergasted]
    #20966071 - 12/12/14 10:08 AM (9 years, 5 months ago)

Quote:

wowimflabbergasted said:
Quote:

blackout said:
Quote:

Kizzle said:
If you were planning to make an LC you can always make the most of your "empty" spawn jars as well.


Good idea, there is nearly always some myc clinging to the glass. People could get in the habit of properly sealing the "empty" jars in their sterile environment and just not bother washing them right away.




That's what my avatar is.



I thought you ran a volt of electricity through that bitch.

That shit looks insane bro. What strain was that?


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InvisibleMunchauzen
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: wowimflabbergasted]
    #20966081 - 12/12/14 10:10 AM (9 years, 5 months ago)

that is some sexy shit right there

:wowz:

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Invisiblewowimflabbergasted
supercalifragilistic
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: taGyo]
    #20966089 - 12/12/14 10:12 AM (9 years, 5 months ago)

GT I think. I forgot about it and found it 2 months after a G2G. Was like:niggawhy:


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OfflineNoobiesnack
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: wowimflabbergasted]
    #20966452 - 12/12/14 11:33 AM (9 years, 5 months ago)

Quote:

wowimflabbergasted said:
Quote:

blackout said:
Quote:

Kizzle said:
If you were planning to make an LC you can always make the most of your "empty" spawn jars as well.


Good idea, there is nearly always some myc clinging to the glass. People could get in the habit of properly sealing the "empty" jars in their sterile environment and just not bother washing them right away.




That's what my avatar is.




Did you add anything after using your spawn jar or did this happen on it's own? I'm kinda curious what prompted this to happen! Awesome picture

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Invisiblewowimflabbergasted
supercalifragilistic
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: Noobiesnack]
    #20966497 - 12/12/14 11:44 AM (9 years, 5 months ago)

Nope. I just put the lid back on and let it sit under my desk for 2 months.


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Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: wowimflabbergasted]
    #20966530 - 12/12/14 11:52 AM (9 years, 5 months ago)

Quote:

wowimflabbergasted said:
Nope. I just put the lid back on and let it sit under my desk for 2 months.





I thought that was sclerotia???


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Invisiblewowimflabbergasted
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: d0urd3n]
    #20966561 - 12/12/14 11:59 AM (9 years, 5 months ago)

:shrug:


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Offlineblackout
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Re: Making the most of your apparently "empty" syringe, making a long lasting LC [Re: wowimflabbergasted]
    #21002011 - 12/20/14 05:49 AM (9 years, 4 months ago)

I did this technique with the syringe and it appears to have given growth.

The vendor has 09/13 on it which I presume is the date its made, it was stored at room temp all this time. I had tried about 1-1.5ml in 2 PF type jars I made, and got no growth at all probably at 5-6 weeks now. I put about 0.5-1ml in a large agar tray and this still has no growth.

These are pictures of the syringe from a few days ago.




It is hard to get a good photo, I had light shining into the syringe. There appeared to be fuzzy growth all over the plunger, this is why it is white looking, its not a trick of the light. In hindsight I should have got a clean syringe so they could be both photographed at once and directly compared under the same light conditions. The second pic is blurry, there was growth suspended in this one as I had swirled the syringe a little.

This has since gone into an LC jar and growth is taking off well. I sucked LC into the syringe and pressed very hard to eject all the myc. I could see the growth lifting off the plunger. I did this several times without taking the needle out of the SHIP.

I have always thought it best to start MS LCs off in smaller volumes of broth. This was a malt extract broth. I figure the spores will not get "lost" in small LC, and you can visually see growth better as its so concentrated. As the growth was all over the plunger I can only guess spores had adhered to it, I do not think it was growth settling on the plunger as when swirled it was still stuck well, and did appear to be growing upwards from it.

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