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OfflineGreekBagel
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Registered: 04/24/14
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Last seen: 8 years, 11 months
Few questions about Agar and liquid cultures
    #20952140 - 12/09/14 11:31 AM (9 years, 5 months ago)

Hi all!

Trying my first go at Agar using the Pasty Plate method! Ordered all my stuff, but I had a few questions:

1. How long will Agar be good for after it is colonized? How long can I keep it (not in a fridge or freezer) before using?

2. If I order Liquid Culture, can I inoculate with the colonized agar? And back to question 1, how long is liquid culture good for? In the jar and in the syringe?

3. I have a previous attempted grow where I accidentally caused Myc Death with a heater, some of it recovered but it did not take. The Myc is still there after a few weeks (not growing), can I take one of the colonized grains and put them in agar/liquid culture? Or has something bad most likely formed by now?

Thanks for all the help in advance!


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MY FIRST GROW
http://www.shroomery.org/forums/showflat.php/Number/20300064


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Offlinemushpunx
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Registered: 04/20/14
Posts: 13,394
Last seen: 5 days, 35 minutes
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Re: Few questions about Agar and liquid cultures [Re: GreekBagel]
    #20952238 - 12/09/14 11:55 AM (9 years, 5 months ago)

You can put a colonized grain on agar and try to seperate out healthy growth. I just did this with a pan cyan culture I couldnt get germination on straight agar with.

Im not sure how long a plate can last in a fridge but if you make a master slant with agar it can keep a culture for years, and into perpetuity with a little maitinence.
Out a the fridge your culture will keep growing out and eat up all the agar, dry out, or contam

You can inoculate LC with agar and many people do, I dont, I drop a wedge straight into my grains.

There is also a newer method which involves blending the agar in sterile water called liquid innoculant


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Amateur Mycologists United
AMU Q&A

Edited by mushpunx (12/09/14 11:57 AM)

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Offlineblackout
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Registered: 07/16/00
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Re: Few questions about Agar and liquid cultures [Re: mushpunx]
    #20954018 - 12/09/14 05:46 PM (9 years, 5 months ago)

Quote:

mushpunx said:There is also a newer method which involves blending the agar in sterile water called liquid innoculant



Nothing new about it, you can see one of the long term vendors Ryche Hawk talking about it in this thread from 14.5years ago
http://www.shroomery.org/forums/showflat.php/Number/22533#22533

another older one here trying to sell a blender and saying how good LI is vs normal agar.
http://www.shroomery.org/forums/showflat.php/Number/92301

The method (agar in blender with water) is clearly spelt out in the mushroom cultivator book which is 30 years old.

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Offlinemushpunx
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Registered: 04/20/14
Posts: 13,394
Last seen: 5 days, 35 minutes
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Re: Few questions about Agar and liquid cultures [Re: blackout]
    #20954058 - 12/09/14 05:54 PM (9 years, 5 months ago)

Yea? I didnt know that!

But what I meant was, and should've said, is a method which has recently increased in popularity

Heh


--------------------

Amateur Mycologists United
AMU Q&A

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