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OfflineDirtysouthhippy
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the right malt extract for agar?
    #20948892 - 12/08/14 06:42 PM (9 years, 5 months ago)

this is the agar I got it says it is Amber I think it is in between light and dark


Is this ok to mix with dextrose and agar to make MEA?

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InvisibleMunchauzen
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Re: the right malt extract for agar? [Re: Dirtysouthhippy]
    #20948922 - 12/08/14 06:49 PM (9 years, 5 months ago)

13 shrooms has a huge list of agar recipes in his journal: http://www.shroomery.org/forums/showflat.php/Number/20868609

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OfflineDirtysouthhippy
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Re: the right malt extract for agar? [Re: Munchauzen]
    #20949005 - 12/08/14 07:04 PM (9 years, 5 months ago)

Recipes are always great thanks. Will this Malt shown in picture above work for the Malt Extract Agar? I would think so, I just want to make sure before I open it.

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InvisibleNumeroEno
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Re: the right malt extract for agar? [Re: Dirtysouthhippy]
    #20949028 - 12/08/14 07:09 PM (9 years, 5 months ago)

Agar is pretty forgiving. I use PDA because I can get all the ingredients from my work. As long as your agar is sterile you will be able to grow mycelium on it :smile:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlineblojo02184
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Re: the right malt extract for agar? [Re: NumeroEno]
    #20949204 - 12/08/14 07:40 PM (9 years, 5 months ago)

yes, it will work.
I usually shoot for the lightest malt extract I can get.

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OfflinePsilicon
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Re: the right malt extract for agar? [Re: blojo02184]
    #20949713 - 12/08/14 09:24 PM (9 years, 5 months ago)

For agar, what you want is a simple sugar, like maltose (malt), glucose (corn sugar) or fructose (corn syrup), in a small quantity--typically less than 2% but often as little as a single drop in 200mL.  You also may want a starch as well, in a quantity between 0% and 8% by weight, although at higher concentrations starch will make your agar stiff.  Potential starch sources are tapioca, potato, corn starch, ground whole grains and ground rice.  Finally you want agar agar in a concentration between 1.5% and 3%, with 1.5% making a very soft agar and 3% making a very stiff agar.

Got it?  More than no simple sugars but less than 2%.  Less than 8% starch.  More than 1.5% agar, less than 3% agar.  Follow any recipe and rest assured that you are not fucking this up.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Invisiblemicelio
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Re: the right malt extract for agar? [Re: Dirtysouthhippy]
    #20949760 - 12/08/14 09:33 PM (9 years, 5 months ago)

Quote:

Dirtysouthhippy said:
this is the agar I got it says it is Amber I think it is in between light and dark


Is this ok to mix with dextrose and agar to make MEA?




I been using amber. It's works find and I would say it's on the light side of the scale.


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OfflinePsilicon
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Re: the right malt extract for agar? [Re: micelio]
    #20949775 - 12/08/14 09:36 PM (9 years, 5 months ago)

They'll eat what you put on their plates and they better be goddamned grateful.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflineDirtysouthhippy
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Re: the right malt extract for agar? [Re: NumeroEno]
    #20951385 - 12/09/14 08:31 AM (9 years, 5 months ago)

Thanks for the info, the sugar, starch, agar ratio is great info for anybody with that you can make 100s or formulations with out a single recipe, Thanks van der griegen and everyone else that contributed:yourock:

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