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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 14 hours
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How did you learn to isolate sectors?
#20934432 - 12/05/14 06:54 PM (9 years, 5 months ago) |
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I dont have much experience yet in isolation, just the agar basics... pouring, cleaning, and cloning.
Now that I have my flow hood built, and many different clean plates laying around its time to start filling up those Pitres and fruiting out strains.
I understand the core concepts, at least I think I do, but I do.
But I dont think I have the best eye for storing yet.
So Im looking for tips, like how big do you usually grow your plate out before you start cutting?
Share your secrets!!
Thx
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 Amateur Mycologists United AMU Q&A
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: How did you learn to isolate sectors? [Re: mushpunx]
#20934450 - 12/05/14 06:58 PM (9 years, 5 months ago) |
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I'm in the same boat. Check out this thread, its very helpful.
Stro's Cleaning and Isolating On Agar
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: How did you learn to isolate sectors? [Re: mushpunx]
#20934452 - 12/05/14 06:59 PM (9 years, 5 months ago) |
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 14 hours
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Re: How did you learn to isolate sectors? [Re: MudaFuka]
#20934468 - 12/05/14 07:01 PM (9 years, 5 months ago) |
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Ahh beautifull links folks much appreaciated
The photo where he highlights and circles the lines of se ctoring really helps!!
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (12/05/14 07:04 PM)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: How did you learn to isolate sectors? [Re: MudaFuka]
#20934497 - 12/05/14 07:09 PM (9 years, 5 months ago) |
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That's a good read. Those transfers are gorgeous. Makes me want to try pour agar and make pretty plates like that. Don't think I wanna fuck with that until I can build a flowhood though.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Re: How did you learn to isolate sectors? [Re: mushpunx]
#20934506 - 12/05/14 07:11 PM (9 years, 5 months ago) |
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Nice flow hood.. Congratulation, your on your way to success.
I'm not an authority on isolates so I'm going to keep an eye on this thread and see what I can learn...
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Jean-guy Masta
Railyard Ghost



Registered: 09/23/14
Posts: 1,827
Loc: MT-Hell
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Re: How did you learn to isolate sectors? [Re: micelio]
#20934516 - 12/05/14 07:14 PM (9 years, 5 months ago) |
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there a vid from lets grow mushroom on isolating sector . im no expert in growing but it looks well explained
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 14 hours
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Re: How did you learn to isolate sectors? [Re: NumeroEno]
#20934557 - 12/05/14 07:23 PM (9 years, 5 months ago) |
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Quote:
EnoRoxxx said: That's a good read. Those transfers are gorgeous. Makes me want to try pour agar and make pretty plates like that. Don't think I wanna fuck with that until I can build a flowhood though.
Nah dude go with the SAB and start learning!
I've seen RR s video and its really good, but I gotta say its pretty intimidating looking at a dish and trying to decipher sectoring at first. At least for me haha
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 Amateur Mycologists United AMU Q&A
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: How did you learn to isolate sectors? [Re: mushpunx]
#20934568 - 12/05/14 07:24 PM (9 years, 5 months ago) |
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Quote:
mushpunx said:
Quote:
EnoRoxxx said: That's a good read. Those transfers are gorgeous. Makes me want to try pour agar and make pretty plates like that. Don't think I wanna fuck with that until I can build a flowhood though.
Nah dude go with the SAB and start learning!
I've seen RR s video and its really good, but I gotta say its pretty intimidating looking at a dish and trying to decipher sectoring at first. At least for me haha
I have a SAB and use it a lot. I've done no pour agar and all that. My SAB is small but it works. A flowhood would be so nice though.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 14 hours
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Re: How did you learn to isolate sectors? [Re: NumeroEno]
#20934594 - 12/05/14 07:30 PM (9 years, 5 months ago) |
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Oh dude it fuckin rules haha. Actually since Ive built it every single plate has been clean, I haven't needed more than one transfer after germination..
I guess my SAB technique is pretty sloppy haha
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 Amateur Mycologists United AMU Q&A
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: How did you learn to isolate sectors? [Re: Jean-guy Masta]
#20934633 - 12/05/14 07:38 PM (9 years, 5 months ago) |
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RR's video and Stro's cleaning & isolating should be all you need.
Personally I never got too bonered up about isolates & monocultures...
Most of the time I got better results from clone cultures than isolates that I spent months working out the sectors... You just never know what you're going to isolate. Now I like to just start with good spores, make a couple transfers and let it rip. Take a clone, transfer a couple, then run it... Collect spores, and do it all over again
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: How did you learn to isolate sectors? [Re: PirateSwazey]
#20934667 - 12/05/14 07:43 PM (9 years, 5 months ago) |
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Yah I don't isolate on purpose. I prefer to clone. All my isolates came inadvertently from making transfers to expand my plates or clean up dirty cultures. It is quite easy to pour plates in a SAB. I do it all the time. The key is to make your SAB tall enough.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: How did you learn to isolate sectors? [Re: MudaFuka]
#20935048 - 12/05/14 09:00 PM (9 years, 5 months ago) |
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Glad Stro is already up here.
That guy's the man
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Enigma1
Positive



Registered: 08/15/13
Posts: 977
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Re: How did you learn to isolate sectors? [Re: mushpunx]
#20935181 - 12/05/14 09:29 PM (9 years, 5 months ago) |
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Quote:
mushpunx said: I dont have much experience yet in isolation, just the agar basics... pouring, cleaning, and cloning.
Now that I have my flow hood built, and many different clean plates laying around its time to start filling up those Pitres and fruiting out strains.
I understand the core concepts, at least I think I do, but I do.
But I dont think I have the best eye for storing yet.
So Im looking for tips, like how big do you usually grow your plate out before you start cutting?
Share your secrets!!
Thx
I personally like to do a MS grow on a dozen of cakes. I take clones from mushrooms that have the traits I want and isolate sectors from my clones. Once I isolate I test on cakes or small trays microwavable Ramon noodle trays to be exact. I grow my fruiting plates out to about the size of a quarter before I fruit because when I tried fruiting my pieces to small the agar dried out to fast before it had a chance to take hold on the seed. Just experiment have fun and be curious on a handful of methods.
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 14 hours
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Re: How did you learn to isolate sectors? [Re: Enigma1]
#20936697 - 12/06/14 08:33 AM (9 years, 5 months ago) |
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Hey thanks for all the tips guys!
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 Amateur Mycologists United AMU Q&A
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 14 hours
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Re: How did you learn to isolate sectors? [Re: MudaFuka]
#20936721 - 12/06/14 08:42 AM (9 years, 5 months ago) |
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Quote:
MudaFuka said: Yah I don't isolate on purpose. I prefer to clone. All my isolates came inadvertently from making transfers to expand my plates or clean up dirty cultures. It is quite easy to pour plates in a SAB. I do it all the time. The key is to make your SAB tall enough.
This is how I have the isolates I already have, right before I knock up my master I expand to a new plate to keep it going or if I have to transfer enough times it ends up pretty limited and sometimes it happens ha
Ive read alot of clone vs isolate threads and it seems to come down to preference.
I think Im just gunna start cutting and and see what I can come up with haha. I figure Ill get it if I play around long enoughAww
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 Amateur Mycologists United AMU Q&A
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: How did you learn to isolate sectors? [Re: mushpunx]
#20936742 - 12/06/14 08:50 AM (9 years, 5 months ago) |
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The "let's grow some dicks" journal in my sig has some neat agar work in it. I started out with 2 plates using tissue samples from the same mushroom. One plate came out clean, but the other one had mold on it so I transferred 2 pretty sectors to new plates. I just want to have plenty of proven fruiting myc on agar. I may isolate one of the plates further for fun, but I really just want to get some of this agar into grains and LC at this point. I think it's time for me to move up to bigger tubs.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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