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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Pasty Agar Tek [Re: taGyo]
#20914354 - 12/01/14 08:16 PM (9 years, 2 months ago) |
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LMAO!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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At 6'3" 240 lbs I am the meanest Patsy ya will ever meet Probably the ugliest too
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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I was waiting impatiently for Pasty to reply to that, thought he might give me some albino shrooms for repayment for that, which I wanted from him at one time, long story, but it ties right back in with this thread, if he recalls, yup that Pasty, what a kidder he can be!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Quote:
Imperfect Iam said:
I was waiting impatiently for Pasty to reply to that
Patience, I had to transfer to some slants
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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A lot of conflicting information on this so what do you guys use to wrap your petris:
Saran wrap, parafilm, micropore?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: taGyo]
#20914602 - 12/01/14 08:58 PM (9 years, 2 months ago) |
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I use glad wrap but that's just because I haven't gotten around to ordering parafilm
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Re: Pasty Agar Tek [Re: MudaFuka]
#20914628 - 12/01/14 09:04 PM (9 years, 2 months ago) |
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Yeah that was my other option, I'll get some tomorrow for my first transfers.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: taGyo]
#20914658 - 12/01/14 09:11 PM (9 years, 2 months ago) |
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If you use glad wrap. Cut the roll into smaller 2" rolls. It makes life much easier.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Re: Pasty Agar Tek [Re: MudaFuka]
#20914686 - 12/01/14 09:18 PM (9 years, 2 months ago) |
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Read that already, Thanks again Muda,
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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twospirit
Facepalm master


Registered: 12/01/14
Posts: 4
Last seen: 8 years, 5 months
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Re: Pasty Agar Tek [Re: taGyo]
#20914986 - 12/01/14 10:39 PM (9 years, 2 months ago) |
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Gonna have to bookmark this one great read and very helpful pasty plates are on the to-do list
-------------------- Humankind has not woven the web of life. We are but one thread within it. Whatever we do to the web, we do to ourselves. All things are bound together. All things connect.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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OI! I made a Violet-Pasty Plate. I put your agar into a Screw Top ZipLoc container... I meant to have a huge amount of this great mycelium I grew, but I made the agar layer so thick, that it's like thicker than a pizza. So that's stupid. 

Normally what I do though is pull the wedges, lay them sideways, cut the excess off of the bottom of the wedge and lay it mycelium side down on the receiving plate. I'll probably be trimming the excess when I inoculate with this, or I'll make a LI with it
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pasty Agar Tek [Re: Pudgy]
#20916961 - 12/02/14 12:50 PM (9 years, 2 months ago) |
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You should not need to trim the excess. That's just more vectors. If it bothers you use less agar per round. Having excess agar is not an issue no matter what you're doing with it. The myc will colonize the excess quickly and the extra nutes will help it to leap off stronger once it does.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Quote:
Pastywhyte said: You should not need to trim the excess. That's just more vectors. If it bothers you use less agar per round. Having excess agar is not an issue no matter what you're doing with it. The myc will colonize the excess quickly and the extra nutes will help it to leap off stronger once it does.
I meant excess in terms of depth of the wedge. The mycelium just colonizes the surface if I'm not mistaken. I prefer to have deeper agar plates because of the storage capabilities. I'm not doing slants yet, nor can I afford to, so short term storage of my plates is necessary. You and others have pointed out thinner agar's downside is definitely the storage capabilities.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Pudgy]
#20917453 - 12/02/14 02:16 PM (9 years, 2 months ago) |
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It colonizes the surface until you cut it or it gets to an edge, then it'll colonize all of it. The thickness of said wedge will never really matter.
There really isn't a problem with thick agar, if you can get a piece of it loose then you've got what you need. Only downside is it can be difficult to get a wedge depending on exactly how thick.
I make thicker plates for cultures I plan on hanging onto for a while as well since I haven't figured out slants yet.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: Inocuole]
#20917470 - 12/02/14 02:19 PM (9 years, 2 months ago) |
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Quote:
Inocuole said: It colonizes the surface until you cut it or it gets to an edge, then it'll colonize all of it. The thickness of said wedge will never really matter.
There really isn't a problem with thick agar, if you can get a piece of it loose then you've got what you need. Only downside is it can be difficult to get a wedge depending on exactly how thick.
I make thicker plates for cultures I plan on hanging onto for a while as well since I haven't figured out slants yet.

I'm so anal retentive I despise tall wedges on my receiving plate surface. I trim them so they are very slim.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Re: Pasty Agar Tek [Re: Inocuole]
#20917557 - 12/02/14 02:31 PM (9 years, 2 months ago) |
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Quote:
Inocuole said: I make thicker plates for cultures I plan on hanging onto for a while as well since I haven't figured out slants yet.
Haven't figured out or haven't tried? What difficulties are you running into?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Pasty Agar Tek [Re: Pudgy]
#20917573 - 12/02/14 02:33 PM (9 years, 2 months ago) |
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Quote:
SuPaTriP said: I personally would much rather prefer disposable petris. But not having a flow hood, pouring a bunchof petris would be a massive pita. Imma roll with the pasty plates till I build my flowhood.
I poured 50 plates today without a flow hood, a flow hood is more of a pain in the ass due to the time it takes to set up and how long you have to leave it running to use it.
Quote:
Inocuole said: It also doesn't sit well with me, coming up in pasty's tek claiming different methods to be superior. "Oh it's not your fault or anything, my tek is just plainly better and I posted it before yours" You can like them better but to just claim them as superior... Just doesn't resonate right.
all based on preference.
I'm sure it doesn't resonate well with a lot of people. I suspect that's why some threads/teks/methods are more or less popular, because of the attitude that goes along with it.
I use saran wrap on my dishes but I've got a roll of parafilm on order now that I work in a lab
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Has anyone tried not snapping the lids on the dishes for the PC? That is, just setting the lid on the dish, then putting the foil over it. I've been doing it since my 2nd or 3rd batch, and its made dealing with the containers a lot easier. I just have to remove the foil, then I can just pick up the lid without any force.
Edited by Munchauzen (12/02/14 02:50 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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I tried that once with the larger Ziploc ones. It got sucked shut while PCing and got crushed. Never tried with the glad ones but they might indeed be better suited due to the smaller size.
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