|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
|
Re: Pasty Agar Tek [Re: SuPaTriP]
#20910322 - 11/30/14 10:18 PM (9 years, 2 months ago) |
|
|
That recipe will do 8 - 10 rounds. A grain to agar is a great way to start isolating or just to get a nice culture with limited genetics
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: SuPaTriP]
#20910337 - 11/30/14 10:24 PM (9 years, 2 months ago) |
|
|
No need for expensive honey. any honey will work. I can make around 30 pasty plates with 500ml of water so you should be abled to get 10 out of 120mls. I would go with the colonised grain. It will get you going much faster. You should be abled to get transferable growth in just a couple of days. With spores it could take weeks just to see growth. Isolating from MS, it will take you a long time and a lot of testing to find a good set of genetics. I recommend starting with an MS grow and cloning some fruits from it. Look fore fruits growing in large clusters to take clones from.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20911039 - 12/01/14 05:30 AM (9 years, 2 months ago) |
|
|
If I'm just dropping spore solution into the agar, is it bad to use a bunch? I've done a single drop to a few and I don't think any viable spores hit because it's been a week and nothing has germinated. Can I just drop like 5-10 in there and then transfer the mess later or will that fuck something up?
I tried digging it in like someone mention before, but it plugs the syringe and makes me squirt WAAAY too much.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
|
Quote:
Pastywhyte said: Well folks, its been a year since I got up the courage to post this. In that time this thread has received over 34,000 views, 1650+ replies, had some great discussions, and I have made some good friends. I look through the boards and I see new growers not intimidated by agar, they are embracing it. Teks have been developed on how to use these rounds. I have received many from people thanking me, asking questions, and just to shoot the shit. I love it. Not to mention that despite my lack of encouragement, the name "pasty plates" seems to have stuck 
This tek would not be the success it has without all of those who come here to help with advice, and use the method themselves. I would like to thank all of you guys and you know who you are. Lets look forward to another year of clones, clean inoculate, and isolates.


never really used pasty plates much but this TEK is such an obvious shroomery cornerstone
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
Quote:
stareatclouds said: If I'm just dropping spore solution into the agar, is it bad to use a bunch? I've done a single drop to a few and I don't think any viable spores hit because it's been a week and nothing has germinated. Can I just drop like 5-10 in there and then transfer the mess later or will that fuck something up?
I tried digging it in like someone mention before, but it plugs the syringe and makes me squirt WAAAY too much.
You can do that but I prefer to use just a drop. It can take more than a week for spores to germinate. I've seen growth in as little as 3 days but I've also had some take 3 weeks.
|
taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
|
|
Quote:
stareatclouds said: If I'm just dropping spore solution into the agar, is it bad to use a bunch? I've done a single drop to a few and I don't think any viable spores hit because it's been a week and nothing has germinated. Can I just drop like 5-10 in there and then transfer the mess later or will that fuck something up?
I tried digging it in like someone mention before, but it plugs the syringe and makes me squirt WAAAY too much.
It'll look like this:

-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: taGyo]
#20911497 - 12/01/14 09:04 AM (9 years, 2 months ago) |
|
|
This is the JAS plate from my spore inoculation video. I used a small amount of spore in one spot on the plate. It took almost 3 weeks to show growth but it looks as it should.

|
Violet



Registered: 12/06/11
Posts: 4,205
|
|
Quote:
Pastywhyte said: Well folks, its been a year since I got up the courage to post this. In that time this thread has received over 34,000 views, 1650+ replies, had some great discussions, and I have made some good friends. I look through the boards and I see new growers not intimidated by agar, they are embracing it. Teks have been developed on how to use these rounds. I have received many from people thanking me, asking questions, and just to shoot the shit. I love it. Not to mention that despite my lack of encouragement, the name "pasty plates" seems to have stuck 
This tek would not be the success it has without all of those who come here to help with advice, and use the method themselves. I would like to thank all of you guys and you know who you are. Lets look forward to another year of clones, clean inoculate, and isolates.

Yeah very nice. Makes me wish I had posted my original no-pour tek (a month before you posted this one, no less), which NO OFFENSE I feel is much simpler and superior (entirely due to the container's use method and variety, no fault of yours of course), in its own thread. Instead I chose to keep everything culturing-related in one tek rather than go OP crazy, and the result is my culturing thread. Sometimes I wonder if separate threads would have drawn more attention total. I still think about breaking them up.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Violet]
#20911588 - 12/01/14 09:36 AM (9 years, 2 months ago) |
|
|
I have used both and I still do with slight variations to each one to make them suit me better. When it comes to which containers are better I Think it really comes down to personal preference. My favorite are the ziploc pop tops. They have much more surface area than the mini rounds but aren't as cumbersome as the screw tops.
|
Violet



Registered: 12/06/11
Posts: 4,205
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20911692 - 12/01/14 10:10 AM (9 years, 2 months ago) |
|
|
I have around 300 of these screw-top Ziplocs and I have never found them to be cumbersome... I have found them to be amazing, perfect, ideal in every way (except clear lids would be the clincher)
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Violet]
#20911739 - 12/01/14 10:34 AM (9 years, 2 months ago) |
|
|
I found one brand that has clear lids but they were a bit flimsy and didn't close as well as the Ziploc. I just find screw tops a bit big for storage and the tall walls make transfers a bit harder. The biggest draw back for me is the price though.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20911746 - 12/01/14 10:37 AM (9 years, 2 months ago) |
|
|
culturing techniques are way easier in the smaller pop tops then the twist offs. the twist offs are good for making pf cakes though PF cake of the same size in a PP5 cup beats out using cased grass seed in one any day.
|
Violet



Registered: 12/06/11
Posts: 4,205
|
|
Quote:
MudaFuka said: I just find screw tops a bit big for storage and the tall walls make transfers a bit harder. The biggest draw back for me is the price though.
They are a bit more pricey, but consider the real meaning of price. Which truly costs more? 65¢ each for a one-trick-pony? Or 90¢ each for EVERYTHING YOU NEED? Unlike most appear to, I consider price very eclectically, and thus I consider mini-rounds a waste. The ONLY thing they do better is occupy less space in refrigeration, and they're far from the best option for that anyway, so IMO it's a relatively moot point...
EDIT: I am reminded of the "tiger drop". For those that have troubles with sterile transfers, and for making Liquid Inoculant, this is the ONE true advantage I see in the mini-rounds.
Seriously don't know what the problem is with twist-tops for some of you peeps. It's said mini-rounds are easier to use... but look at all the extra materials and tricks that are required for their sterilization and use up to inoculation. I do nothing but sterilize with slightly loose lids; good to go. I've never seen anything easier in my life.
I'm glad that this thread is so effective at getting people in on agar, but in my experience and in my strong opinion, once peeps are in on teks there's a lot of dead wait to drop from most of them. Modifications and micropore tape to make up for a snap-top lid (which has essentially no real advantage)? Paper towel to protect the micropore tape? Foil to protect the towel? Come on...
Of course I use brown rice or grass seed grainwater agar so really all my work involves is putting the right measurement of agar-agar powder into the grainwater, loading 20-25mL into each container, applying lids loosely and sterilizing. Done.
Also. I've made unimaginably more transfers with the screw-tops, taller sides and all, than pretty much anybody. There are few cultivators active on these boards that I wouldn't claim to have made more transfers Period than... Including you, muda. This is no vie for superiority. It's only meant as a support for the following statement: I have absolutely no problem making transfers. It is only be slightly more tricky to learn than any transfer.
Quote:
bodhisatta said: PF cake of the same size in a PP5 cup beats out using cased grass seed in one any day.
Factually incorrect
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: Pasty Agar Tek [Re: Violet]
#20911987 - 12/01/14 11:44 AM (9 years, 2 months ago) |
|
|
can't be factually incorrect considering people have had varying results.
my best cased seed cup, and this was after culturing for set of genetics that performed well in this way of growing

 pails in comparison to the yield of a PF cake of the same size. 
not to mention the biggest benefit is not getting your yields spread out among 3+ flushes, a cake pops out most of your BE on the first 2 flushes
the smaller pop top cups are good for test cakes too, you can fruit many more test cultures in the same sized space.
Edited by Trusted cuItivator (12/01/14 11:46 AM)
|
Violet



Registered: 12/06/11
Posts: 4,205
|
|
Quote:
bodhisatta said: can't be factually incorrect considering people have had varying results.
my best cased seed cup

 pails in comparison to the yield of a PF cake of the same size. 
If nothing else, that you said brf/verm beats watered cased grains "any day" is factually incorrect, because results DO vary! Duh! I, for starters, experienced the reverse Every Day.
There is significantly more dry nutritious substrate in a half-full pint pp5 than in a brf/verm cake of the same size. There is more than DOUBLE the dry nutritious sub in a whole brown rice substrate (than brf/verm) of the same volume. Why are your results realized weaker than the potential? Ask yourself that before you post here.
When I can get 11-17 grams dry from grass seed containers, and 13-24 grams dry from cased brown rice, I cannot hear someone tell me that the quite outstanding (rather rare) accomplishment of more than 10 grams from a PF cake somehow "beats out" watered whole grain cakes. Especially considering the much more troublesome handling and ineffective fruiting environment PF cakes require.
So "varying results" is truly no different than the varying results from one grow to the next, and the varying results that come with improvement.
That's a nice cup you posted. I it looks nicer than it really is... I've seen you post it before. Somewhat short mushrooms, probably kindof hollow, for a not-as-impressive dry weight. THAT is were "results vary." You can do better, and I'm surprised you haven't by now. Keep that in mind before YOU are claiming superior teks, because I can't help but figure that you're using individual grower's brief experiences in one tek to attempt to undermine biological facts as a whole.
We can debate this (elsewhere) if you wish, but you'll lose. .... Why are we even talking about this? Because you took a snide shot at my tek, like you will NEVER pass on an opportunity to do? This is a culturing tek. Stick to that topic.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: Pasty Agar Tek [Re: Violet]
#20912042 - 12/01/14 11:57 AM (9 years, 2 months ago) |
|
|
you can fit nearly 2 cakes worth of cake substrate into a screw off pint and easily get 24 dry grams on the first two flushes. and for whatever reason they consistently produce better with MS genetics than the grain cups do with MS, why is beyond my scope. and that's in the same fruiting chamber
results vary. try both, I did. I stuck with what I like. now I'm trying the muda jars, and they're promising as well
Edited by Trusted cuItivator (12/01/14 11:58 AM)
|
Violet



Registered: 12/06/11
Posts: 4,205
|
|
Well you're not actually talking about
Quote:
bodhisatta said: PF cakes of the same size
then, are you?
Sorry your results didn't reach the potential. This is your problem, not the forum's. Your claims fall short of what I've shown is possible and easy. Other people here have seen it, and other people have gotten the results you failed to however many(read:few) times you may have tried. Don't refer to your experience as a proof of anything.
Quote:
cronicr said: BD has it easy, violet had teks posted with great results and went through hell and back
You're behind the times on this Bod. We'll never know why you keep stirring up the same tired old shit with me.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: Pasty Agar Tek [Re: Violet]
#20912087 - 12/01/14 12:06 PM (9 years, 2 months ago) |
|
|
well in that case then I would say everyone go look for the results of either way and then try them both ways yourself. Most of the people that I frequently talk with have shared similar results to me so I'm inclined one way and I give advice in the way I'm inclined. I'm not pulling anyone's leg here. At worst I'm telling them to try both ways. Hey I'm not the one saying I think my way is superior. How can one way be superior, that's for the individual to decide. I'm just sharing my experience. seems like you're pushing yours which blows my mind to why that's your agenda all the time.
Quote:
Violet said: which NO OFFENSE I feel is much simpler and superior (entirely due to the container's use method and variety, no fault of yours of course
PS. threads and forums are for debate. so complaining about debating over and over is your straw-man argument, it's not really what we're talking about, you came in here and said screw tops are superior you could have just said hey everyone check out this other way of doing things, see if you like it.
Edited by Trusted cuItivator (12/01/14 12:08 PM)
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Violet]
#20912096 - 12/01/14 12:08 PM (9 years, 2 months ago) |
|
|
I don't like the mini rounds that much either I like the mid sized Ziploc pop tops. They have the same surface area as a petri dish. The walls are high enough to use them for no pour but low enough that they are easily transferred to and from They have the best visibility of the three. I don't use paper towel. I find it really easy to poke a hole and cover it with a small ball of poly and some tape. Since I have been using the same containers for over 30 runs I only had to modify the lids once any way. Where I live the price difference is much greater. It costs me about $1.60 for 1 screw top, about $0.40 for 1 mini round or around $0.60 for a pop top Ziplocs. The extra cost just isn't worth it to me since I dislike using screw top for agar. I still use screw tops and mini rounds when that's all I have available and they all work. It's just a mater of preference. We are all different and like different things. To each there own. There is no wright or wrong answer here.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20912099 - 12/01/14 12:10 PM (9 years, 2 months ago) |
|
|
to be honest I personally like neither anyway. screw tops or pop tops. try jars and disposable polystyrene dishes. pour agar FTW
Edited by Trusted cuItivator (12/01/14 12:10 PM)
|
|