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thisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 18 years, 10 months
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h202 in g2g
#2085560 - 11/08/03 11:00 PM (20 years, 3 months ago) |
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thanks to whoever posted the idea of keeping a 1/2 pint jar filled with h202 in the glove box and dipping the spoon in that before each scoop. It was just the right amount and allowed for a 60% success rate which was a big leap from the 0% success had as of late.
-------------------- Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.
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stara
addict

Registered: 05/14/03
Posts: 473
Last seen: 8 years, 2 months
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Re: h202 in g2g [Re: thisone]
#2086058 - 11/09/03 05:48 AM (20 years, 3 months ago) |
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I even don't use a glow box,only a high place from bottom:)
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Hanky
wiffle bat.

Registered: 08/30/03
Posts: 56,993
Loc: Great Southern Land.
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Re: h202 in g2g [Re: stara]
#2088268 - 11/10/03 12:43 AM (20 years, 3 months ago) |
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^^^^^what does that mean in english?
-------------------- Coaster is an idiot... [quote]Coaster said: but i thnk everything thats pure is white? [/quote]
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thisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 18 years, 10 months
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Re: h202 in g2g [Re: Hanky]
#2088271 - 11/10/03 12:44 AM (20 years, 3 months ago) |
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I was trying to figure the same thing out.
-------------------- Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.
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stara
addict

Registered: 05/14/03
Posts: 473
Last seen: 8 years, 2 months
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Re: h202 in g2g [Re: thisone]
#2089001 - 11/10/03 10:09 AM (20 years, 3 months ago) |
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I think if you want to do something sterile and you don't have a glowbox you better work in a high table or shelve to avoid flying contaminaters.(english to strict teachers volume :2)That's my method ,anyway,both dealing with sterile methods and english.
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micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: h202 in g2g [Re: stara]
#2089052 - 11/10/03 10:31 AM (20 years, 3 months ago) |
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Damn flying contaminaters.... hehe
No, honestly -- if you don't want to fuck with gloveboxes, etc., why not use bags and make inoculum with a syringe inside of the bag? A place isn't sterile just because it's high up -- especially not for something like g2g.
I didn't like the idea of g2g transfers in my apartment, so I worked around it 
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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stara
addict

Registered: 05/14/03
Posts: 473
Last seen: 8 years, 2 months
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Re: h202 in g2g [Re: micro]
#2089409 - 11/10/03 12:56 PM (20 years, 3 months ago) |
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better than mine I select yours if with a syringe from now on.Thanx.
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stoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 20 years, 1 month
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Re: h202 in g2g [Re: stara]
#2089669 - 11/10/03 02:31 PM (20 years, 3 months ago) |
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If the spoon is your problem, why not just cut out the spoon all together??? Beat your fully colonized cake against a tire until its broken up into small kernels. You could then simply dump some spawn into your virgin jars. And since youd have a bunch of little kernels youd have a lot of innoculation points.
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stara
addict

Registered: 05/14/03
Posts: 473
Last seen: 8 years, 2 months
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Because you use a fully colonized grain jar for g2g it's less sensitive to contamination problems isn't it?I think the problem may occur because of the sterilized grain jar,am I right?Thanks for this thread ,anyway.
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