|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
I'd just bucket tek that shit right in the pool and just cover it with 8 mil poly.
|
MrGiraffe

Registered: 04/04/14
Posts: 3,149
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20890473 - 11/25/14 07:15 PM (9 years, 2 months ago) |
|
|
The biggest problem would be getting enough hot water to reach and hold pasteurization temps, with winter here the basement has dropped to the low to mid 60s and being in direct contact with the concrete floor would suck even more heat out of it. I guess with a high enough spawn ratio and a coir/verm/gypsum mix 140f out of the tap might be good enough to beat out any contams. Shit I've got one of those 2.5mil plastic painter drop cloths and a 25'x10' roll of 8 mil hanging out down there too. Haha stop egging me on, i just broke out the tape measure to get a better visualization of the foot print, that would be one sweet canopy.
--------------------
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
|
|
No worries guys, the thread already is at 33K views
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
That's awesome. I bet it beets the strain thread some day. My hot tap water comes out over 160. If I turn up my hot water heater I can probably get it over 175. If I mix that with a couple big pots of boiling water it should work fine.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20890507 - 11/25/14 07:25 PM (9 years, 2 months ago) |
|
|
Trying a cheaper and somewhat taller variety of containers right now with some black agar. Hadn't seen black yet and I bet that shows contams the absolute best. And maybe I'm a little biased.( )
Hopefully the lids don't melt and everything goes smoothly. I'll take some pics of those once they're done. Are higher walls of the container really such a bad thing? Seems like a contam shield to me.
Edited by Inocuole (11/25/14 07:30 PM)
|
Munchauzen


Registered: 06/22/11
Posts: 14,343
|
Re: Pasty Agar Tek [Re: Inocuole]
#20890526 - 11/25/14 07:29 PM (9 years, 2 months ago) |
|
|
well, petri dishes are ideal, and they have tiny walls
the taller the wall, the longer your instrument will have to be and the more risk you run of passing a hand over the plate. the walls of the normal ones already force me to tilt my xacto knife at an angle. much more of an angle and my hand would be over the agar, which would be bad.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Inocuole]
#20890531 - 11/25/14 07:30 PM (9 years, 2 months ago) |
|
|
I made black once. It stained the shit out of the mycelium. I just pick my pasty plates up and tilt them rather than getting my hands too close.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
Quote:
Munchauzen said: well, petri dishes are ideal, and they have tiny walls
the taller the wall, the longer your instrument will have to be and the more risk you run of passing a hand over the plate. the walls of the normal ones already force me to tilt my xacto knife at an angle. much more of an angle and my hand would be over the agar, which would be bad.
That is bad, but is tilting the container while working with it bad practice, and if not, wouldn't that be ideal?
Quote:
MudaFuka said: I just pick my pasty plates up and tilt them rather than getting my hands too close.
Ah, well, there's that. In fact, what if that were part of your procedure, to tilt it almost completely sideways and then extract it using a long tool so that nothing is ever above the agar? I thought that's how you interact with slants?
|
Munchauzen


Registered: 06/22/11
Posts: 14,343
|
Re: Pasty Agar Tek [Re: Inocuole]
#20890548 - 11/25/14 07:36 PM (9 years, 2 months ago) |
|
|
that is not a bad idea
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
|
|
That's how I've always taken my chunks of agar out. And when I photograph them, I hold them upside down or at a completely vertical angle to shield them from any still falling contaminants. I thought everyone tilted theirs to some degree when scooping/stabbing the wedges out?
The issue I'm having is that the depth of the substrate is so much that it's nearly impossible for me to get a good angle with my knife to take the full chunk out at once. Instead, it just rips it in half and I wind up needing 5 transfers to get it all in my LI jar. It sucks. I'm thinking I need to make my agar a bit thicker in each plate as well.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
|
|
I usually hold them at a pretty steep angle when using them.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
Well, I think I saw Muda's video and he kind of took the lid off of his donor plate and left it off to the side while the plate was sitting wide open during the transfer. I don't know if that's better or worse procedure than holding the lid and the plate together in one hand and being able to close them immediately after you take the sample but before you open the lid to the receiving plate.
I kind of took that order of steps as being like "Fuck the donor plate, it doesn't matter what happens to it" while focusing on the more swift and effective technique for the recipient plate.
In that sense I'm a little bit torn up about what I should be doing with lids with my left hand while the other hand has a tool with mycelium on it. In that moment it seems like you have to make a sacrifice somewhere in your technique to do the least harm, depending on what is most important, and I guess that's going to work differently for each of us.
Edited by Inocuole (11/25/14 07:59 PM)
|
taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
|
Re: Pasty Agar Tek [Re: Inocuole]
#20890634 - 11/25/14 07:56 PM (9 years, 2 months ago) |
|
|
My agar is dark purple so I beat you to it Inoc 
That is if they work.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Inocuole]
#20890645 - 11/25/14 07:58 PM (9 years, 2 months ago) |
|
|
Those videos were not a good example of sterile tek at all. I was half asleep and in a rush. I throw out my donor plates after I transfer from them so the lid doesn't even need to go back on.
Edited by MudaFuka (11/25/14 07:59 PM)
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20890676 - 11/25/14 08:03 PM (9 years, 2 months ago) |
|
|
Well, good. Because I've only done that with donor plates that I didn't give a shit about. I find that I'm having a hard time getting spores from a syringe to germinate on the agar though. I have some AA+ mycelium going strong and spiky already but the PF classic either won't germinate or gets that really fast bacteria that colors the whole top a cloudy translucent white.
I'm betting prints are 100x better for agar. The only germination I got was from where the syringe shot out a giant glob of spores right on the plate. 
On that note, anybody wanna make a video of their best attempt at a simple transfer between pasty plates? If nobody else will, I'll give it a shot, but I'm the one asking all these questions so it would be a little silly unless the idea was to critique it.
|
Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
|
Re: Pasty Agar Tek [Re: Inocuole]
#20890746 - 11/25/14 08:21 PM (9 years, 2 months ago) |
|
|
Quote:
Inocuole said: In that sense I'm a little bit torn up about what I should be doing with lids with my left hand while the other hand has a tool with mycelium on it. In that moment it seems like you have to make a sacrifice somewhere in your technique to do the least harm, depending on what is most important, and I guess that's going to work differently for each of us. 
For me at least, sacrificing the mycelium on the tool is the best approach. I hold the donor plate and its lid in my left hand, barely open the lid, remove some myc with the tool in my right hand, close the lid on the donor plate, sit the donor plate down, pick up the receiving plate and open it (already loosened) just far enough to get the tool in to place the transfer. My reasoning is if I'm taking multiple samples from the donor plate I want it to say clean through the whole process. The myc on the tool is usually just one of many transfers so if something happens to get on it in the time it's hovering in the air it's no big loss. Also a wedge doesn't have much surface area for contams to land on, but the plates, those have a lot of surface area and can pick up contams easier than a little wedge on the end of a blade can.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
i wanna use black agar, i think that would be ideal, or dark blue or something
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
Quote:
Elf_on_a_Log said:
Quote:
Inocuole said: In that sense I'm a little bit torn up about what I should be doing with lids with my left hand while the other hand has a tool with mycelium on it. In that moment it seems like you have to make a sacrifice somewhere in your technique to do the least harm, depending on what is most important, and I guess that's going to work differently for each of us. 
For me at least, sacrificing the mycelium on the tool is the best approach. I hold the donor plate and its lid in my left hand, barely open the lid, remove some myc with the tool in my right hand, close the lid on the donor plate, sit the donor plate down, pick up the receiving plate and open it (already loosened) just far enough to get the tool in to place the transfer. My reasoning is if I'm taking multiple samples from the donor plate I want it to say clean through the whole process. The myc on the tool is usually just one of many transfers so if something happens to get on it in the time it's hovering in the air it's no big loss. Also a wedge doesn't have much surface area for contams to land on, but the plates, those have a lot of surface area and can pick up contams easier than a little wedge on the end of a blade can.
I keep the lid in my hand for multiple transfers. The video inocuole mentioned was actually of making an LI not a transfer.
|
Munchauzen


Registered: 06/22/11
Posts: 14,343
|
Re: Pasty Agar Tek [Re: MudaFuka]
#20890778 - 11/25/14 08:30 PM (9 years, 2 months ago) |
|
|
I have made orange, yellow, red, and blue.
So far I recommend orange.
|
stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
|
Re: Pasty Agar Tek [Re: Inocuole]
#20890787 - 11/25/14 08:32 PM (9 years, 2 months ago) |
|
|
Quote:
On that note, anybody wanna make a video of their best attempt at a simple transfer between pasty plates? If nobody else will, I'll give it a shot, but I'm the one asking all these questions so it would be a little silly unless the idea was to critique it.
It will benefit everyone for sure. I'd appreciate if you recorded one. I would be down as well, although I think it should be in a separate thread. Despite the fact we use this as everything related to agar in Pasty plates, I don't think tons of newer members check it out because it's so big.
Also Muda's video (which was an LI, not a transfer) definitely showed him holding the plate up and a bit tilted when cutting it out.
|
|