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filamentous
Entheomycologist
Registered: 01/20/14
Posts: 422
Last seen: 8 years, 3 months
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Transplanting genes from fungi into bacteria (psilocin related)
#20883244 - 11/24/14 11:38 AM (9 years, 3 months ago) |
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Quote:
Replicating the production of psilocin in a suitable bacterium likely requires only to transplant one gene, that being the tryptamine-4-hydroxylase. It should be possible to produce massive quantities of baeocystin in a bioreactor in a very similar way to the production of tryptophan. The biosythesis of psilocybin proceeds by the following route: tryptophan --> tryptamines --> 4-hydroxy-tryptamines --> psilocin --> psilocybin.
See: http://actachemscand.org/pdf/acta_vol_22_p1210-1218.pdf http://libgen.org/scimag/get.php?doi=10.1002%2Fjobm.3620290608
I found this over at the vesp. Does anyone know that gene? Anybody know a suitable bacteria to use(bacillus)? Anyone know the genes responsible of the other fungal tryptamines like baeocystin, psilosamuicin, etc?
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drake89
Mushroom Magnate
Registered: 06/26/11
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: filamentous]
#20884319 - 11/24/14 04:18 PM (9 years, 3 months ago) |
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Sounds risky. You could grow shrooms without millions in R&D
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44teddyb
Psychonaut
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: drake89]
#20885860 - 11/24/14 08:53 PM (9 years, 3 months ago) |
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Bacteria mutate a lot because of their short life spans so I'm not sure how viable it would stay for the long term but for the first generations of bacteria it sounds like it would be a very promising way to get a supply of psilocybin for say medical research uses but I agree with the last guy who posted that it would probably be a lot of money to find the correct gene and find a way to splice it into the bacteria's genes when you could just grow a shit ton of shrooms. Although this would lead to almost instantaneous psilocybin so no more waiting for the shrooms to grow. Definitely would like to hear what people have to think and no sorry sad to say I don't know the answer to any of the original ?'s.
-------------------- I dont do drugs I AM drugs -Salvador Dali I loved when Bush came out and said, 'We are losing the war against drugs.' You know what that implies? There's a war being fought, and the people on drugs are winning it. - Bill Hicks
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Alan Rockefeller
Mycologist
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: filamentous]
#20887051 - 11/25/14 01:14 AM (9 years, 3 months ago) |
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As far as I know, the full genome of a psilocybin mushroom has not been sequenced, and the gene has not been identified.
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44teddyb
Psychonaut
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: Alan Rockefeller]
#20890569 - 11/25/14 07:42 PM (9 years, 3 months ago) |
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I know what I'm doin when I'm done with college
-------------------- I dont do drugs I AM drugs -Salvador Dali I loved when Bush came out and said, 'We are losing the war against drugs.' You know what that implies? There's a war being fought, and the people on drugs are winning it. - Bill Hicks
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Amphibolos
Le bourgeois gentilhomme
Registered: 05/22/09
Posts: 626
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: 44teddyb]
#20897824 - 11/27/14 05:38 PM (9 years, 3 months ago) |
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It wont be profitable to do this since we can get psilocybin readily from a source that is easily grown. Nobody will invest a couple of thousands for something like this imho.
-------------------- "Homo sum ; humani nihil a me alienum puto"
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filamentous
Entheomycologist
Registered: 01/20/14
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: Amphibolos]
#20899494 - 11/28/14 06:42 AM (9 years, 3 months ago) |
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Quote:
Amphibolos said: It wont be profitable to do this since we can get psilocybin readily from a source that is easily grown. Nobody will invest a couple of thousands for something like this imho.
It won't be profitable? It's not about profits. It's about having an easier route to get to psilocin, and getting it faster as to get more people turned on. Nobody will invest a couple of thousands? Monotubs done by different members on this site beg to differ.
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Amphibolos
Le bourgeois gentilhomme
Registered: 05/22/09
Posts: 626
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: filamentous] 1
#20900224 - 11/28/14 12:08 PM (9 years, 3 months ago) |
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Think what you want, thoughts are far from the acts.
These kind of threads get posted every 2 months, and so far nobody has done anything in this sense.
Like Alan also said in earlier posts, the genome of a psilocybe specie hasnt even been sequenced yet. So work on this first, then we can start to build something with the knowledge.
Also, doing a bunch of monotubs costing less than 10$ each to make is far different than setting a proper biotech lab and doing repeated (costly) experiments in it. Not that i want to ruin it for you guys, if you are motivated and the people want to pitch in, go for it. We managed to get a good bunch of money for the pestalotiopsis microspora so i guess we could try and crowd fund the sequencing of a psilocybe specie. After its getting people from the community to analyse and annote the sequence.
-------------------- "Homo sum ; humani nihil a me alienum puto"
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44teddyb
Psychonaut
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: Amphibolos]
#20902745 - 11/28/14 11:00 PM (9 years, 3 months ago) |
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well my major is biochemistry heavy on the bio side so ill get back to you in a few years when I graduate and have money to do something like this.
-------------------- I dont do drugs I AM drugs -Salvador Dali I loved when Bush came out and said, 'We are losing the war against drugs.' You know what that implies? There's a war being fought, and the people on drugs are winning it. - Bill Hicks
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nooneman
Registered: 04/24/09
Posts: 14,665
Loc: Utah
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: filamentous]
#20902769 - 11/28/14 11:06 PM (9 years, 3 months ago) |
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Easier route to psilocin? Easier than cubes? Cubes are easier than growing a plant. They require almost no effort at all.
Edited by nooneman (11/28/14 11:07 PM)
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filamentous
Entheomycologist
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: nooneman]
#20903285 - 11/29/14 03:25 AM (9 years, 3 months ago) |
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Bacteria would need even less attention than cubes. Faster biosynthesis too.
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nooneman
Registered: 04/24/09
Posts: 14,665
Loc: Utah
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: filamentous]
#20903400 - 11/29/14 05:34 AM (9 years, 3 months ago) |
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Bacteria would be harder to culture than cubensis.
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drake89
Mushroom Magnate
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: nooneman]
#20903609 - 11/29/14 08:20 AM (9 years, 3 months ago) |
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Have you looked at what just a bioreactor costs? Not cheap...Plus youd spend a mint searching for this gene or genes that code for this protein or enzyme. Got any heady millionaire friends?
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: drake89]
#20903810 - 11/29/14 09:42 AM (9 years, 3 months ago) |
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-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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44teddyb
Psychonaut
Registered: 01/25/14
Posts: 214
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: blindingleaf]
#20911607 - 12/01/14 09:41 AM (9 years, 3 months ago) |
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Lol I actually do. My mom works for this guy who is a millionaire many times over and he's gotten shrooms from me many times on the dl lmfao. I'm sure he'd be willing to fund a little research and he's getting older to so he doesn't have a lot to do with his $.
-------------------- I dont do drugs I AM drugs -Salvador Dali I loved when Bush came out and said, 'We are losing the war against drugs.' You know what that implies? There's a war being fought, and the people on drugs are winning it. - Bill Hicks
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hidyn
Noob
Registered: 07/14/13
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Loc: Canada
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: 44teddyb]
#20913872 - 12/01/14 06:53 PM (9 years, 3 months ago) |
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I did see a few videos not too long ago about replicating genes with Bacteria. The process was really simple, well within the realm of DIY.
Funny that this particular project didn't occur to me...
-------------------- My Trade List!
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tokeweed420
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: hidyn]
#20914981 - 12/01/14 10:37 PM (9 years, 3 months ago) |
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44teddyb
Psychonaut
Registered: 01/25/14
Posts: 214
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: hidyn]
#20915771 - 12/02/14 06:34 AM (9 years, 3 months ago) |
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That isn't the hard part though because to find the gene that needs to be transplanted you would need to have a psilocybin producing mushroom's genome fully mapped to know what genes need to be transplanted. And mapping a genome isn't a small task.
-------------------- I dont do drugs I AM drugs -Salvador Dali I loved when Bush came out and said, 'We are losing the war against drugs.' You know what that implies? There's a war being fought, and the people on drugs are winning it. - Bill Hicks
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Alan Rockefeller
Mycologist
Registered: 03/10/07
Posts: 48,355
Last seen: 15 hours, 49 minutes
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: 44teddyb]
#20915909 - 12/02/14 07:49 AM (9 years, 3 months ago) |
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Quote:
44teddyb said: That isn't the hard part though because to find the gene that needs to be transplanted you would need to have a psilocybin producing mushroom's genome fully mapped to know what genes need to be transplanted. And mapping a genome isn't a small task.
Not too hard either, costs about $2000. The harder part would be to identify which gene codes for the tryptamine pathways.
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filamentous
Entheomycologist
Registered: 01/20/14
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Re: Transplanting genes from fungi into bacteria (psilocin related) [Re: Alan Rockefeller]
#20916803 - 12/02/14 12:19 PM (9 years, 3 months ago) |
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Let's get this $2k crowd funded then
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