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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20881340 - 11/23/14 08:58 PM (9 years, 3 months ago)

Did that mycelium eventually change phase once it hit the bulk sub or was it wispy like that the whole way through?  There seems to be a general thing with slower cultures producing bigger or more potent fruits, and faster ones producing smaller fruits, but I just wouldn't expect a flush like that unless the tomentose appearance is just something that happens on the agar.

All of my APE mycelium has been really thick, I have some AA+ that's looking to be pretty thick as well.  I've yet to see any growth like your second picture in my plates.  Also how did the speed compare?


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InvisibleImperfect Iam
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Re: Pasty Agar Tek [Re: Imperfect Iam]
    #20881345 - 11/23/14 08:59 PM (9 years, 3 months ago)

That's not to say the enoki(given as an example in above post) will only pin when cold shocked, but I do know it helps.


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20881354 - 11/23/14 09:01 PM (9 years, 3 months ago)

I've yet to see a strong looking rhizomorphic culture like that first plate, but I also cut them out long before they get to that size, so maybe that plays a part. I know rhizomorphic mycelium isn't the end all be all for this, but I can't help but wonder why mine are so fluffy and apparently tomentose?

Do you know what species that one was? And was it transferred a few times to get there?


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20881567 - 11/23/14 09:41 PM (9 years, 3 months ago)

It's a cube and it looks like a monoculture. So I'm guessing it was transferred more than once. If you really want to see rhizos use less nutes in your agar.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20881804 - 11/23/14 10:40 PM (9 years, 3 months ago)

Here are the jars I inoculated with that plate.



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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20881823 - 11/23/14 10:45 PM (9 years, 3 months ago)

Oh shit is that your rusty white?


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20881837 - 11/23/14 10:49 PM (9 years, 3 months ago)

It was. Need to start another of those soon.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20881861 - 11/23/14 10:59 PM (9 years, 3 months ago)

Quote:

MudaFuka said:
Oh shit is that your rusty white?



Quote:

Pastywhyte said:
It was. Need to start another of those soon.




Some backstory?


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: Inocuole]
    #20881905 - 11/23/14 11:12 PM (9 years, 3 months ago)

Check the grow logs link in my sig :thumbup:


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20881935 - 11/23/14 11:20 PM (9 years, 3 months ago)

Oh cool so did you get that by intersecting them on agar and taking the resulting third sector and fruiting it?


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: Inocuole]
    #20881946 - 11/23/14 11:24 PM (9 years, 3 months ago)

Nope I just took two prints that were both fresh and scraped up spores from both with the same loop. Then one transfer to max the amount of genetics and looked for stuff I wanted


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20881962 - 11/23/14 11:28 PM (9 years, 3 months ago)

Whoa.

I see.  I guess that's one way of doing it.  I have a lot of work to do I guess.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflinetaGyo
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20882690 - 11/24/14 09:05 AM (9 years, 2 months ago)

Quote:

MudaFuka said:
It's a cube and it looks like a monoculture. So I'm guessing it was transferred more than once. If you really want to see rhizos use less nutes in your agar.



Would I mess with the amount of instant potato flakes to drop nutrients?

Also,
Wouldn't this culture just turn tomentose once it hits my rye grains which should have plenty of nutes?


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OfflinePudgy
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Re: Pasty Agar Tek [Re: Imperfect Iam]
    #20882800 - 11/24/14 09:33 AM (9 years, 2 months ago)

Quote:

Imperfect Iam said:
@ Pudgy, you may have already heard this somewhere else, but it has been true IME that if you introduce lighting to the agar, (sometimes throwing it in the fridge will do it too, in cultures that require a cold shock before fruiting, example, enoki) sometimes it will pin for you, I have a plate right now pinning, a few days after being exposed to light, and the same culture in a different dish (in the dark) not doing shit.




I am going to electrocute a substrate soon... Once I have a little bit more money, I'm going to build a 50,000 kV taser and shock a sub.

I don't have a monoculture yet so that has to come first, but once I've got a mono -- I'm gonna do all kinds of tests on it. Music, electroshock, positivity vs negativity and weird shit like that. FOR FUN RIGHT?!


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Pudgy]
    #20882808 - 11/24/14 09:35 AM (9 years, 2 months ago)

Quote:

Pudgy said:
Quote:

Imperfect Iam said:
@ Pudgy, you may have already heard this somewhere else, but it has been true IME that if you introduce lighting to the agar, (sometimes throwing it in the fridge will do it too, in cultures that require a cold shock before fruiting, example, enoki) sometimes it will pin for you, I have a plate right now pinning, a few days after being exposed to light, and the same culture in a different dish (in the dark) not doing shit.




I am going to electrocute a substrate soon... Once I have a little bit more money, I'm going to build a 50,000 kV taser and shock a sub.

I don't have a monoculture yet so that has to come first, but once I've got a mono -- I'm gonna do all kinds of tests on it. Music, electroshock, positivity vs negativity and weird shit like that. FOR FUN RIGHT?!



Keep me posted!

:raisemyglass:


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: taGyo]
    #20882833 - 11/24/14 09:42 AM (9 years, 2 months ago)

Quote:

taGyo said:
Quote:

MudaFuka said:
It's a cube and it looks like a monoculture. So I'm guessing it was transferred more than once. If you really want to see rhizos use less nutes in your agar.



Would I mess with the amount of instant potato flakes to drop nutrients?

Also,
Wouldn't this culture just turn tomentose once it hits my rye grains which should have plenty of nutes?




Sometimes a drop in nutes can promote sectoring or rhizo states making sectoring easier to see. Though IME rhizo can also be from the type of nutrition not just the amount. Simple sugars vs complex can change the appearance based on the cultures preference. I often see more rhizomorphic growth once transferred to grains.


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20882842 - 11/24/14 09:45 AM (9 years, 2 months ago)

Quote:

Pastywhyte said:
Sometimes a drop in nutes can promote sectoring or rhizo states making sectoring easier to see. Though IME rhizo can also be from the type of nutrition not just the amount. Simple sugars vs complex can change the appearance based on the cultures preference. I often see more rhizomorphic growth once transferred to grains.



MORE you say?
I'll look into the sugars. Got some ziplock twist pp5 caps and I'm gonna play with one of my APE syringes tomorrow after it's warmed up from being in the fridge :].
APE spores clumps are WHITE too, that's AWESOME, just gave 'em a look now.


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Invisiblehamloaf
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Re: Pasty Agar Tek [Re: taGyo]
    #20884990 - 11/24/14 06:19 PM (9 years, 2 months ago)

Sterilizing some MYEA to pour 25 single section petri dishes tonight when I got to thinking.  I believe it was in this thread where we were discussing prices of agar. 

I and another poster from I believe the other poster was from Canada were both getting 2 ounces of food grade agar powder for roughly the same price (7-8$ per 2 ounces).  Started to do the math on that.  It's 40 dollars for a pound of agar to buy pre-made and mixed, lab grade agar from fungi perfecti. 

There are 28 grams in an ounce.  I get 2 ounces of powdered food grade agar for 7 dollars for 2 ounces.  To figure what I am currently paying for just food grade powdered agar by itself you multiply 7 (the cost of agar for 2 ounces) by 14.  That equals 96 dollars for a pound of food grade agar by itself.  Not to mention the gas and time it takes to gather the agar, so at half the price I have decided that the next time I order some petri dishes I am going to go ahead and order some of that lab grade, pre-mixed agar for half the price, and have it delivered right along with my petri dishes.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: hamloaf]
    #20885012 - 11/24/14 06:22 PM (9 years, 2 months ago)

Can you no-pour that shit though?


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: Inocuole]
    #20885031 - 11/24/14 06:25 PM (9 years, 2 months ago)

Not with petris but yes you can do no pour with premix.


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