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Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 12 years, 30 days
h202 in g2g
    #2085560 - 11/09/03 01:00 AM (13 years, 5 months ago)

thanks to whoever posted the idea of keeping a 1/2 pint jar filled with h202 in the glove box and dipping the spoon in that before each scoop. It was just the right amount and allowed for a 60% success rate which was a big leap from the 0% success had as of late.


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Offlinestara
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Registered: 05/14/03
Posts: 473
Last seen: 1 year, 4 months
Re: h202 in g2g [Re: thisone]
    #2086058 - 11/09/03 07:48 AM (13 years, 5 months ago)

I even don't use a glow box,only a high place from bottom:)


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InvisibleHanky
wiffle bat.
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Registered: 08/30/03
Posts: 56,993
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Re: h202 in g2g [Re: stara]
    #2088268 - 11/10/03 02:43 AM (13 years, 5 months ago)

^^^^^what does that mean in english?


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[quote]Coaster said:
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Offlinethisone
the one theydon't see

Registered: 11/06/02
Posts: 818
Last seen: 12 years, 30 days
Re: h202 in g2g [Re: Hanky]
    #2088271 - 11/10/03 02:44 AM (13 years, 5 months ago)

I was trying to figure the same thing out.


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Offlinestara
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Registered: 05/14/03
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Re: h202 in g2g [Re: thisone]
    #2089001 - 11/10/03 12:09 PM (13 years, 5 months ago)

I think if you want to do something sterile and you don't have a glowbox you better work in a high table or shelve to avoid flying contaminaters.(english to strict teachers volume :2)That's my method ,anyway,both dealing with sterile methods and english.


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Invisiblemicro
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Registered: 05/09/03
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Re: h202 in g2g [Re: stara]
    #2089052 - 11/10/03 12:31 PM (13 years, 5 months ago)

Damn flying contaminaters....  hehe

No, honestly -- if you don't want to fuck with gloveboxes, etc., why not use bags and make inoculum with a syringe inside of the bag?  A place isn't sterile just because it's high up -- especially not for something like g2g.

I didn't like the idea of g2g transfers in my apartment, so I worked around it :wink:

--
Micro


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Offlinestara
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Re: h202 in g2g [Re: micro]
    #2089409 - 11/10/03 02:56 PM (13 years, 5 months ago)

better than mine I select yours if with a syringe from now on.Thanx.


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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 13 years, 3 months
Re: h202 in g2g [Re: stara]
    #2089669 - 11/10/03 04:31 PM (13 years, 5 months ago)

If the spoon is your problem, why not just cut out the spoon all together??? Beat your fully colonized cake against a tire until its broken up into small kernels. You could then simply dump some spawn into your virgin jars. And since youd have a bunch of little kernels youd have a lot of innoculation points.


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Offlinestara
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Re: h202 in g2g [Re: stoner2002]
    #2089845 - 11/10/03 05:37 PM (13 years, 5 months ago)

Because you use a fully colonized grain jar for g2g it's less sensitive to contamination problems isn't it?I think the problem may occur because of the sterilized grain jar,am I right?Thanks for this thread ,anyway.


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Amazon Shop for: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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