My SAB (Still Air Box) - Mushroom Cultivation

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SpitballJedi
Ancient Astronaut

Registered: 10/13/12
Posts: 8,598
Loc: Nibiru

Re: My SAB (Still Air Box)

[Re: SpitballJedi]
#20847334 - 11/16/14 06:41 PM (9 years, 4 months ago)

I updated the OP with the following:

Getting Ready For Doing Work

I lay the towel down on the table. I have large bath sized towels, so I fold it over. Then, I spray the towel with soap water. I usually use Dr Bronner's Almond Scented Magic Soap. It's what I shower with, but any soap will do. It doesn't take much, just a couple of drops per liter of water. The soap just keeps the water from evaporating too quickly and gives it a little stickiness. I don't see the point in sanitizing the table because it's going to have a towel over it, but do it if it makes you feel good.

Then, I clean the walls of the SAB with 70% IPA. After that, I give a spritz of soap water on the walls and turn the SAB upside down on the towel. Contaminants will stick to wet surfaces. I find soapy water works best. Bleach irritates my nose and lungs. Alcohol dries fast and is flammable.

You can either have the jars or whatever on the towel and cover with the box or feed everything through the arm-holes; it doesn't matter at all.

Once my stuff is in, I let everything just settle for a few minutes, maybe 2-5 minutes. Then, I wipe all my jars or whatever with 70% IPA.

Tips

As Already mentioned, the point of a SAB is to have a place of still air to do sterile work. We call it sterile work because you want to keep your sterilized stuff sterile. The inside of the SAB is not sterile, just still. I put the SAB in the least drafty room, close the door, and turn off anything that creates drafts or air circulation.

Let things settle before wiping down jars in your SAB.

I wear nitrile exam gloves and tyvek sleeves. I actually wear the top half of a tvek suit with the legs cut off. You can just wear a clean long-sleeve shirt though. When not in use, I keep the tyvek in a plastic bags just to keep the dust off. I actually keep a lot of stuff wrapped up in bags or totes to keep the dust off.

Do all flame sterilizations outside the SAB. Heat from the flame creates enough current inside the SAB to cause contams to float around and get in to your sterile work.

It's a fact, attaching gloves to your SAB turns it in to a Glove-box. Attaching the gloves causes more air to move around so therefore, by definition, it is no longer a Still Air Box. I'm sure there are all kinds of ways to prevent contaminated air from coming in to your GB, but you better make sure the inside is already sterile or it will be pointless. Good luck getting it sterile.

Do your work toward the back of the SAB to help prevent contams from getting in your sterile work via the arm holes.

Work diligently. Don't get in a hurry or work at a snails pace. Make all your movements deliberately. Getting in a hurry can stir up the air inside. Working too slow leaves you open for higher potential of getting contams.

Crack the lids on jars before you put them in the SAB. A stuck lid is a bitch to open inside it.

You don't have to shower the walls. You just want a little spritz. Too much, especially in front, will make it hard to see what you're doing. If you are doing much work or working with warm agar, you'll notice condensation building up on the walls anyway. There shouldn't be many contams floating around and it doesn't take much to trap them.

Keep movement over open sterile containers at a bare minimum. I don't like to sit anything dow. This takes a little practice, planning, and dexterity. Try dry runs without any media or anything to see how it feels. Just use some empty jars, bottles, and petris.

One last thing: Always flame sterilize outside the SAB

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