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OfflineMadadaM
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Registered: 10/22/03
Posts: 75
Last seen: 11 years, 6 months
what inocculation method do you use?
    #2084551 - 11/08/03 06:28 PM (13 years, 28 days ago)

oven tek, glove box or something else?


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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: what inocculation method do you use? [Re: MadadaM]
    #2084585 - 11/08/03 06:38 PM (13 years, 28 days ago)

I use rubbing alcohol and syringes.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflineSuntzu
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Re: what inocculation method do you use? [Re: MadadaM]
    #2084701 - 11/08/03 07:30 PM (13 years, 28 days ago)

Cheap glovebox, hepa running ~15 minutes before work:



grain to grain, mycelium slurry, spore water, whatever.


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OfflineRandolph_Carter
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Registered: 06/14/00
Posts: 29,281
Loc: Shroomery B-list.
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Re: what inocculation method do you use? [Re: MadadaM]
    #2084990 - 11/08/03 09:37 PM (13 years, 28 days ago)

Bathroom, bleach, hepa, syringes with spores, syringes with mycellium, mycellium from cakes, half colonized jars, whatever i have on hand. There is nothing in my bathroom that can survive the cell-lysing hell i put it thru.
Stay Frosty.
R.C.


--------------------
"..all those molecules thrashing their kinky little tails, hot for destiny and the street."  Gibson


Nuke baby seals for Jesus!

(This has been a +1 production.)


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Invisiblevampirism
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Registered: 03/14/04
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Re: what inocculation method do you use? [Re: MadadaM]
    #2085623 - 11/09/03 01:51 AM (13 years, 28 days ago)

nice ol glovebox


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OfflineMadadaM
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Registered: 10/22/03
Posts: 75
Last seen: 11 years, 6 months
Re: what inocculation method do you use? [Re: ]
    #2087695 - 11/10/03 12:02 AM (13 years, 27 days ago)

SUNTZU: any horror stories using only cheap glove box?


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InvisibleZildjian
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Registered: 09/11/02
Posts: 208
Loc: new zealand
Re: what inocculation method do you use? [Re: MadadaM]
    #2088679 - 11/10/03 08:44 AM (13 years, 27 days ago)

kitchen bench, near compost bin full of many types of mould and bacteria, meths down bench, hands and any tools and hold breath during innoculations.

I had a glove box, but it wasn't any better than the kitchen bench.


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OfflineChromeCrow
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Re: what inocculation method do you use? [Re: Zildjian]
    #2089201 - 11/10/03 01:43 PM (13 years, 27 days ago)

for innoculatrion of grains NO protection other than holding breathe and flaming between each jar. for syringe making and agar and g to g i use a homemade still air glove box.. works fine and only a 8 dollar investment...... ok, im a cheap ass


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OfflineMadadaM
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Registered: 10/22/03
Posts: 75
Last seen: 11 years, 6 months
Re: what inocculation method do you use? [Re: ChromeCrow]
    #2089250 - 11/10/03 02:02 PM (13 years, 27 days ago)

so what your saying is that when you use a syringe as long as you hold your breath and flame the needle, you should be fine? what is your success rate using this philosophy? a lot of us might be giving ourselves headaches trying to make a perfectly sterile inviornment.



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OfflineChromeCrow
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Re: what inocculation method do you use? [Re: MadadaM]
    #2090356 - 11/10/03 08:45 PM (13 years, 26 days ago)

as an example i finished 3 cases + 3 jars( 39 qt. jars total at one time) , i was sitting here at my pc desk ,and got NO contams


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InvisibleMrPink
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Registered: 10/10/03
Posts: 124
Re: what inocculation method do you use? [Re: ChromeCrow]
    #2091619 - 11/11/03 08:36 AM (13 years, 26 days ago)

I simply run a HEPA filter in my closet for 20 minutes and get to work. It's worked very well... I rarely get contaminations.


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OfflineSuntzu
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Re: what inocculation method do you use? [Re: MrPink]
    #2091713 - 11/11/03 10:07 AM (13 years, 26 days ago)

I agree with this. If you address the general air quality of the area in which you'll be working, your odds of success go way up. Hepa units are a great investment, and make life a lot easier.


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InvisibleMrPink
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Registered: 10/10/03
Posts: 124
Re: what inocculation method do you use? [Re: Suntzu]
    #2091877 - 11/11/03 11:56 AM (13 years, 26 days ago)

I also want to add that this investment only costed me 60 bucks. Here's the particular HEPA unit that I use

http://www.amazon.com/exec/obidos/tg/det...4#more-pictures

After letting it run for 20 minutes I merely wipe the table down and my hands down with a few sprays of isopropyl alcohol... that's all there is to it.


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OfflineMadadaM
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Registered: 10/22/03
Posts: 75
Last seen: 11 years, 6 months
Re: what inocculation method do you use? [Re: MrPink]
    #2092398 - 11/11/03 02:33 PM (13 years, 26 days ago)

you have room in your closet for a table? Damn!


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Offlinekingkc
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Registered: 04/25/00
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Last seen: 2 days, 22 hours
Re: what inocculation method do you use? [Re: MadadaM]
    #2107258 - 11/14/03 07:38 PM (13 years, 22 days ago)

Open air scooping up of spores onto a knife blade then dumping into sterile media.


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Invisibledoktor_alternate
card-carryingAmateurMycologistsAnonymous member
Registered: 08/24/03
Posts: 119
Loc: vancouver
secret inocculation method tek. [Re: kingkc]
    #2110018 - 11/15/03 10:06 PM (13 years, 21 days ago)

here is what here is what i like to do. im only telling you because you seem like a nice guy and i wanna help you out. its a secret though, so dont tell anyone else, ok?

the secret is starting with fresh
mushrooms.
now these dont have to be specail mushrooms, just go to the grocery store and get some of the cheapest ones you can find. (or get some expensive ones with the five-finger discount! but for best results get them all old and gross-looking)

then go home and grind the mushrooms into a paste using a mortar and pestle, adding peroxide to get the consistiency right. if you dont have a mortar and pestle, you can use your mouth just brush your teeth before you do it!

it should feel kind of like fresh poop. i like to take a little bit of my poop out to feel it as i am working just to be sure its right. then take this poop-like paste (be sure you dont accidentally use your test poop. i have dont this before and ended up with some wack shit (pun intended) that really fucked me up!!!) anyway, the point is you wanna make your regular mushrooms into drug mushrooms, right? so then take some weed. use the best weed you can find! and mix it 50/50 with the paste. you gotta grind this too until its all uniform.

now take this paste and mix it with your substrate after you sterilise the substrate. use a spoonful of paste for every jar of substrate. poop works good as a substrate. i find best results with my own poop after ive been doing lots of drugs the previous night cuz it gets more drugs into the mushrooms. then incubate at 80F until theyre all colonized and birth them in your terrarium.

its that easy!


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Invisibledoktor_alternate
card-carryingAmateurMycologistsAnonymous member
Registered: 08/24/03
Posts: 119
Loc: vancouver
Re: secret inocculation method tek. [Re: doktor_alternate]
    #2110024 - 11/15/03 10:07 PM (13 years, 21 days ago)

look at all the neat things you can learn by asking questions in the advanced forum!


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Invisiblemicro
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: secret inocculation method tek. [Re: doktor_alternate]
    #2110029 - 11/15/03 10:08 PM (13 years, 21 days ago)

I don't think you can clone mushrooms unless they're < 48 hrs old....

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Invisiblesimplemachine
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Registered: 09/14/03
Posts: 1,981
Re: secret inocculation method tek. [Re: micro]
    #2110043 - 11/15/03 10:18 PM (13 years, 21 days ago)

no dude with the shit and weed tek u can clone ANYTHING


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Invisiblesimplemachine
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Re: secret inocculation method tek. [Re: simplemachine]
    #2110046 - 11/15/03 10:19 PM (13 years, 21 days ago)

Oh yea I use oven tek all the time with nearly no contams


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