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Offlinellamaboy
the weasel thatsnagged the bee

Registered: 11/08/03
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syringes and agar?
    #2084122 - 11/08/03 03:43 PM (13 years, 29 days ago)

i'm currently thinking about starting some azure agar cultures...however, all the strains i want of azure are only available in syringe form for some reason. i was wondering if it is possible to start an agar culture from a syringe?


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Offlineshirley knott
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Re: syringes and agar? [Re: llamaboy]
    #2084212 - 11/08/03 04:19 PM (13 years, 29 days ago)

of course. spores to agar. agar to grain. grain to woodchips. outside. badading badaboom


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buh


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OfflineRohypnol
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Registered: 08/04/03
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Re: syringes and agar? [Re: shirley knott]
    #2084270 - 11/08/03 04:35 PM (13 years, 29 days ago)

I have a quetsion pertaining to the agar to grain phase. Is there is way to do this in a more sterile way than just opening the jar (thereby compromising sterility) and dumping it in?


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Invisiblekezgin
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Re: syringes and agar? [Re: Rohypnol]
    #2084497 - 11/08/03 06:05 PM (13 years, 29 days ago)

use a flowhood, glove box or the oven tek


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Offlinellamaboy
the weasel thatsnagged the bee

Registered: 11/08/03
Posts: 563
Loc: Portland PNW
Last seen: 7 years, 1 month
Re: syringes and agar? [Re: kezgin]
    #2085609 - 11/09/03 01:40 AM (13 years, 29 days ago)

i guess i should clarify what my concern with using a syringe for agar is: in order to get enough spores onto the agar would the water used to deliver the spores do anything damaging to the agar? or would i not need to deliver say a whole cc as you would with a jar?


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Invisiblevampirism
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Re: syringes and agar? [Re: llamaboy]
    #2085613 - 11/09/03 01:42 AM (13 years, 29 days ago)

1cc is probably too much, any amount will work for agar, really.. just try to get it spread out as much as possible.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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